Muerte celular inducida por parasporinas y péptidos sintéticos de la parasporina-6 de Bacillus thuringiensis en células de cáncer de cuello uterino y su relación con la vía de adenilato ciclasa

Cervical cancer is one of the main diseases in women worldwide, multiple non and invasive therapies have been used, such as chemotherapy and hysterectomy, wspecific hich derive cells of noncancerous lineage. For this reason, specific alternative therapies have been studied, such as treatment with pa...

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Autores:
Guevara Medina, Cesar Julian
Tipo de recurso:
Trabajo de grado de pregrado
Fecha de publicación:
2022
Institución:
Universidad Antonio Nariño
Repositorio:
Repositorio UAN
Idioma:
spa
OAI Identifier:
oai:repositorio.uan.edu.co:123456789/7108
Acceso en línea:
http://repositorio.uan.edu.co/handle/123456789/7108
Palabra clave:
Cáncer
cérvix
parasporinas
HeLa
Bacillus thuringiensis
Cancer
cervix
parasporin
HeLa
Bacillus thuringiensis
Rights
openAccess
License
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
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dc.title.es_ES.fl_str_mv Muerte celular inducida por parasporinas y péptidos sintéticos de la parasporina-6 de Bacillus thuringiensis en células de cáncer de cuello uterino y su relación con la vía de adenilato ciclasa
title Muerte celular inducida por parasporinas y péptidos sintéticos de la parasporina-6 de Bacillus thuringiensis en células de cáncer de cuello uterino y su relación con la vía de adenilato ciclasa
spellingShingle Muerte celular inducida por parasporinas y péptidos sintéticos de la parasporina-6 de Bacillus thuringiensis en células de cáncer de cuello uterino y su relación con la vía de adenilato ciclasa
Cáncer
cérvix
parasporinas
HeLa
Bacillus thuringiensis
Cancer
cervix
parasporin
HeLa
Bacillus thuringiensis
title_short Muerte celular inducida por parasporinas y péptidos sintéticos de la parasporina-6 de Bacillus thuringiensis en células de cáncer de cuello uterino y su relación con la vía de adenilato ciclasa
title_full Muerte celular inducida por parasporinas y péptidos sintéticos de la parasporina-6 de Bacillus thuringiensis en células de cáncer de cuello uterino y su relación con la vía de adenilato ciclasa
title_fullStr Muerte celular inducida por parasporinas y péptidos sintéticos de la parasporina-6 de Bacillus thuringiensis en células de cáncer de cuello uterino y su relación con la vía de adenilato ciclasa
title_full_unstemmed Muerte celular inducida por parasporinas y péptidos sintéticos de la parasporina-6 de Bacillus thuringiensis en células de cáncer de cuello uterino y su relación con la vía de adenilato ciclasa
title_sort Muerte celular inducida por parasporinas y péptidos sintéticos de la parasporina-6 de Bacillus thuringiensis en células de cáncer de cuello uterino y su relación con la vía de adenilato ciclasa
dc.creator.fl_str_mv Guevara Medina, Cesar Julian
dc.contributor.advisor.spa.fl_str_mv Losada Barragán, Mónica
dc.contributor.author.spa.fl_str_mv Guevara Medina, Cesar Julian
dc.subject.es_ES.fl_str_mv Cáncer
cérvix
parasporinas
HeLa
Bacillus thuringiensis
topic Cáncer
cérvix
parasporinas
HeLa
Bacillus thuringiensis
Cancer
cervix
parasporin
HeLa
Bacillus thuringiensis
dc.subject.keyword.es_ES.fl_str_mv Cancer
cervix
parasporin
HeLa
Bacillus thuringiensis
description Cervical cancer is one of the main diseases in women worldwide, multiple non and invasive therapies have been used, such as chemotherapy and hysterectomy, wspecific hich derive cells of noncancerous lineage. For this reason, specific alternative therapies have been studied, such as treatment with parasporins (PS) extracted from Bacillus thuringiensis (Bt) and different anticancer peptides (ACPs). In this work, the cy totoxic effect, and morphological changes present in HeLa cells by pure protein extracts of different Bt strains and synthetic peptides derived from PS6 called PS6DONE and PS6DTWO were evaluated. Additionally, cAMP levels in HeLa cells treated with the two peptides were quantified to determine their relationship with the adenylate cyclase (AC) signaling pathway by means of a direct competitive ELISA assay. The results show a high cytotoxic effect presented by protein extracts from parasporal inclusions agai nst the HeLa cell line associated with necrosis. On the other hand, a cytotoxic effect of the PS6DONE peptide and an increase in cAMP levels were observed, which were accompanied by morphological changes that suggested an autophagy process.
publishDate 2022
dc.date.accessioned.none.fl_str_mv 2022-10-12T16:58:02Z
dc.date.available.none.fl_str_mv 2022-10-12T16:58:02Z
dc.date.issued.spa.fl_str_mv 2022-07-08
dc.type.spa.fl_str_mv Trabajo de grado (Pregrado y/o Especialización)
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dc.identifier.uri.none.fl_str_mv http://repositorio.uan.edu.co/handle/123456789/7108
dc.identifier.bibliographicCitation.spa.fl_str_mv Appleby P, Beral V, Berrington de Gonzalez A, et al. Carcinoma of the cervix and tobacco smoking: collaborative reanalysis of individual data on 13,541 women with carcinoma of the cervix and 23,017 women without carcinoma of the cervix from 23 epidemiological studies. Int J Cancer. 2006;118(6):1481–1495
Bhatla, N., Aoki, D., Sharma, D. N., & Sankaranarayanan, R. (2018). Cancer of the cervix uteri. International Journal of Gynecology & Obstetrics, 143, 22–36. https://doi.org/10.1002/ijgo.12611
Bhatla, N., Berek, J. S., Cuello Fredes, M., Denny, L. A., Grenman, S., Karunaratne, K., Kehoe, S. T., Konishi, I., Olawaiye, A. B., Prat, J., & Sankaranarayanan, R. (2019). Revised FIGO staging for carcinoma of the cervix uteri. International Journal of Gynecology & Obstetrics, 145(1), 129–135. https://doi.org/10.1002/ijgo.12749
Brueggemann, L., Cribbs, L., Schwartz, J., Wang, M., Kouta, A., & Byron, K. (2018). Mechanisms of PKA-Dependent Potentiation of Kv7.5 Channel Activity in Human Airway Smooth Muscle Cells. International Journal of Molecular Sciences, 19(8), 2223. https://doi.org/10.3390/ijms19082223
Castella, C., Pauron, D., Hilliou, F., Trang, V. T., Zucchini-Pascal, N., Gallet, A., & Barbero, P. (2019). Transcriptomic analysis of Spodoptera frugiperda Sf9 cells resistant to Bacillus thuringiensis Cry1Ca toxin, reveals that extracellular Ca2+, Mg2+ and production of cAMP are involved in toxicity. Biology Open. https://doi.org/10.1242/bio.037085
Crickmore, N.; Zeigler, D.R.; Schnepf, E.; Rie, J.; Lereclus, D.; Baum, J.; Bravo, A.; Dean, D.H. Bacillus thuringiensis Toxin Nomenclature. Available online: http://www.lifesci.sussex.ac.uk/home/Neil_Crickmore/Bt/ (Acceso en marzo de 2021).
Deng, H., Hillpot, E., Mondal, S., Khurana, K. K., & Woodworth, C. D. (2018). HPV16Immortalized Cells from Human Transformation Zone and Endocervix are More Dysplastic than Ectocervical Cells in Organotypic Culture. Scientific Reports, 8(1). https://doi.org/10.1038/s41598-018-33865-2
Deng, H., Mondal, S., Sur, S., & Woodworth, C. D. (2019). Establishment and optimization of epithelial cell cultures from human ectocervix, transformation zone, and endocervix optimization of epithelial cell cultures. Journal of Cellular Physiology, 234(6), 76837694. https://doi.org/10.1002/jcp.28049
Elmore, S. (2007). Apoptosis: A Review of Programmed Cell Death. Toxicologic Pathology, 35(4), 495–516. https://doi.org/10.1080/01926230701320337
Epand, R. M., & Vogel, H. J. (1999). Diversity of antimicrobial peptides and their mechanisms of action. Biochimica et Biophysica Acta (BBA) - Biomembranes, 1462(1–2), 1128. https://doi.org/10.1016/s0005-2736(99)00198-4
Felício, M. R., Silva, O. N., Gonçalves, S., Santos, N. C., & Franco, O. L. (2017). Peptides with Dual Antimicrobial and Anticancer Activities. Frontiers in Chemistry, 5. https://doi.org/10.3389/fchem.2017.00005
dc.identifier.instname.spa.fl_str_mv instname:Universidad Antonio Nariño
dc.identifier.reponame.spa.fl_str_mv reponame:Repositorio Institucional UAN
dc.identifier.repourl.spa.fl_str_mv repourl:https://repositorio.uan.edu.co/
url http://repositorio.uan.edu.co/handle/123456789/7108
identifier_str_mv Appleby P, Beral V, Berrington de Gonzalez A, et al. Carcinoma of the cervix and tobacco smoking: collaborative reanalysis of individual data on 13,541 women with carcinoma of the cervix and 23,017 women without carcinoma of the cervix from 23 epidemiological studies. Int J Cancer. 2006;118(6):1481–1495
Bhatla, N., Aoki, D., Sharma, D. N., & Sankaranarayanan, R. (2018). Cancer of the cervix uteri. International Journal of Gynecology & Obstetrics, 143, 22–36. https://doi.org/10.1002/ijgo.12611
Bhatla, N., Berek, J. S., Cuello Fredes, M., Denny, L. A., Grenman, S., Karunaratne, K., Kehoe, S. T., Konishi, I., Olawaiye, A. B., Prat, J., & Sankaranarayanan, R. (2019). Revised FIGO staging for carcinoma of the cervix uteri. International Journal of Gynecology & Obstetrics, 145(1), 129–135. https://doi.org/10.1002/ijgo.12749
Brueggemann, L., Cribbs, L., Schwartz, J., Wang, M., Kouta, A., & Byron, K. (2018). Mechanisms of PKA-Dependent Potentiation of Kv7.5 Channel Activity in Human Airway Smooth Muscle Cells. International Journal of Molecular Sciences, 19(8), 2223. https://doi.org/10.3390/ijms19082223
Castella, C., Pauron, D., Hilliou, F., Trang, V. T., Zucchini-Pascal, N., Gallet, A., & Barbero, P. (2019). Transcriptomic analysis of Spodoptera frugiperda Sf9 cells resistant to Bacillus thuringiensis Cry1Ca toxin, reveals that extracellular Ca2+, Mg2+ and production of cAMP are involved in toxicity. Biology Open. https://doi.org/10.1242/bio.037085
Crickmore, N.; Zeigler, D.R.; Schnepf, E.; Rie, J.; Lereclus, D.; Baum, J.; Bravo, A.; Dean, D.H. Bacillus thuringiensis Toxin Nomenclature. Available online: http://www.lifesci.sussex.ac.uk/home/Neil_Crickmore/Bt/ (Acceso en marzo de 2021).
Deng, H., Hillpot, E., Mondal, S., Khurana, K. K., & Woodworth, C. D. (2018). HPV16Immortalized Cells from Human Transformation Zone and Endocervix are More Dysplastic than Ectocervical Cells in Organotypic Culture. Scientific Reports, 8(1). https://doi.org/10.1038/s41598-018-33865-2
Deng, H., Mondal, S., Sur, S., & Woodworth, C. D. (2019). Establishment and optimization of epithelial cell cultures from human ectocervix, transformation zone, and endocervix optimization of epithelial cell cultures. Journal of Cellular Physiology, 234(6), 76837694. https://doi.org/10.1002/jcp.28049
Elmore, S. (2007). Apoptosis: A Review of Programmed Cell Death. Toxicologic Pathology, 35(4), 495–516. https://doi.org/10.1080/01926230701320337
Epand, R. M., & Vogel, H. J. (1999). Diversity of antimicrobial peptides and their mechanisms of action. Biochimica et Biophysica Acta (BBA) - Biomembranes, 1462(1–2), 1128. https://doi.org/10.1016/s0005-2736(99)00198-4
Felício, M. R., Silva, O. N., Gonçalves, S., Santos, N. C., & Franco, O. L. (2017). Peptides with Dual Antimicrobial and Anticancer Activities. Frontiers in Chemistry, 5. https://doi.org/10.3389/fchem.2017.00005
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dc.publisher.faculty.spa.fl_str_mv Facultad de Ciencias
dc.publisher.campus.spa.fl_str_mv Bogotá - Circunvalar
institution Universidad Antonio Nariño
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spelling Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)Acceso abiertohttps://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Losada Barragán, MónicaGuevara Medina, Cesar Julian118218198382022-10-12T16:58:02Z2022-10-12T16:58:02Z2022-07-08http://repositorio.uan.edu.co/handle/123456789/7108Appleby P, Beral V, Berrington de Gonzalez A, et al. Carcinoma of the cervix and tobacco smoking: collaborative reanalysis of individual data on 13,541 women with carcinoma of the cervix and 23,017 women without carcinoma of the cervix from 23 epidemiological studies. Int J Cancer. 2006;118(6):1481–1495Bhatla, N., Aoki, D., Sharma, D. N., & Sankaranarayanan, R. (2018). Cancer of the cervix uteri. International Journal of Gynecology & Obstetrics, 143, 22–36. https://doi.org/10.1002/ijgo.12611Bhatla, N., Berek, J. S., Cuello Fredes, M., Denny, L. A., Grenman, S., Karunaratne, K., Kehoe, S. T., Konishi, I., Olawaiye, A. B., Prat, J., & Sankaranarayanan, R. (2019). Revised FIGO staging for carcinoma of the cervix uteri. International Journal of Gynecology & Obstetrics, 145(1), 129–135. https://doi.org/10.1002/ijgo.12749Brueggemann, L., Cribbs, L., Schwartz, J., Wang, M., Kouta, A., & Byron, K. (2018). Mechanisms of PKA-Dependent Potentiation of Kv7.5 Channel Activity in Human Airway Smooth Muscle Cells. International Journal of Molecular Sciences, 19(8), 2223. https://doi.org/10.3390/ijms19082223Castella, C., Pauron, D., Hilliou, F., Trang, V. T., Zucchini-Pascal, N., Gallet, A., & Barbero, P. (2019). Transcriptomic analysis of Spodoptera frugiperda Sf9 cells resistant to Bacillus thuringiensis Cry1Ca toxin, reveals that extracellular Ca2+, Mg2+ and production of cAMP are involved in toxicity. Biology Open. https://doi.org/10.1242/bio.037085Crickmore, N.; Zeigler, D.R.; Schnepf, E.; Rie, J.; Lereclus, D.; Baum, J.; Bravo, A.; Dean, D.H. Bacillus thuringiensis Toxin Nomenclature. Available online: http://www.lifesci.sussex.ac.uk/home/Neil_Crickmore/Bt/ (Acceso en marzo de 2021).Deng, H., Hillpot, E., Mondal, S., Khurana, K. K., & Woodworth, C. D. (2018). HPV16Immortalized Cells from Human Transformation Zone and Endocervix are More Dysplastic than Ectocervical Cells in Organotypic Culture. Scientific Reports, 8(1). https://doi.org/10.1038/s41598-018-33865-2Deng, H., Mondal, S., Sur, S., & Woodworth, C. D. (2019). Establishment and optimization of epithelial cell cultures from human ectocervix, transformation zone, and endocervix optimization of epithelial cell cultures. Journal of Cellular Physiology, 234(6), 76837694. https://doi.org/10.1002/jcp.28049Elmore, S. (2007). Apoptosis: A Review of Programmed Cell Death. Toxicologic Pathology, 35(4), 495–516. https://doi.org/10.1080/01926230701320337Epand, R. M., & Vogel, H. J. (1999). Diversity of antimicrobial peptides and their mechanisms of action. Biochimica et Biophysica Acta (BBA) - Biomembranes, 1462(1–2), 1128. https://doi.org/10.1016/s0005-2736(99)00198-4Felício, M. R., Silva, O. N., Gonçalves, S., Santos, N. C., & Franco, O. L. (2017). Peptides with Dual Antimicrobial and Anticancer Activities. Frontiers in Chemistry, 5. https://doi.org/10.3389/fchem.2017.00005instname:Universidad Antonio Nariñoreponame:Repositorio Institucional UANrepourl:https://repositorio.uan.edu.co/Cervical cancer is one of the main diseases in women worldwide, multiple non and invasive therapies have been used, such as chemotherapy and hysterectomy, wspecific hich derive cells of noncancerous lineage. For this reason, specific alternative therapies have been studied, such as treatment with parasporins (PS) extracted from Bacillus thuringiensis (Bt) and different anticancer peptides (ACPs). In this work, the cy totoxic effect, and morphological changes present in HeLa cells by pure protein extracts of different Bt strains and synthetic peptides derived from PS6 called PS6DONE and PS6DTWO were evaluated. Additionally, cAMP levels in HeLa cells treated with the two peptides were quantified to determine their relationship with the adenylate cyclase (AC) signaling pathway by means of a direct competitive ELISA assay. The results show a high cytotoxic effect presented by protein extracts from parasporal inclusions agai nst the HeLa cell line associated with necrosis. On the other hand, a cytotoxic effect of the PS6DONE peptide and an increase in cAMP levels were observed, which were accompanied by morphological changes that suggested an autophagy process.El cáncer de cuello uterino es una de las principales enfermedades en mujeres a nivel mundial, se han utilizado múltiples terapias no específicas e invasivas como la quimioterapia e histerectomía, que afectan células de linaje no cancerígeno. Por ello se han estudiado terapias alternativas específicas como el tratamiento con parasporinas (PS) extraídas de thuringiensis (Bt) y diferentes péptidos anticancerígenos (ACPs). En este trabajo, se Bacillus evalúa el efecto citotóxico y los cambios morfológicos presentes en células HeLa por extractos de inclusiones parasporales de diferentes cepas de Bt y péptidos sintéticos derivados de PS6 denominados PS6DONE y PS6DTWO. Adicionalmente, se cuantifican los ni veles de AMPc en células tratadas con el PS6DONE para determinar su relación con la vía de señalización del adenilato ciclasa (AC) por medio de un ELISA. Los resultados muestran un alto efecto citotóxico 3 presentado por los extractos de proteínas de inclusi asociados a necrosis ones parasporales frente a la línea celular HeLa . Por otra parte, se evidenció un efecto citotóxico del péptido PS6DONE y aumento de los niveles de AMPc, que fueron acompañados por cambios morfológicos que sugieren un proceso de autofa gia.Bioquímico(a)PregradoPresencialInvestigaciónspaUniversidad Antonio NariñoBioquímicaFacultad de CienciasBogotá - CircunvalarCáncercérvixparasporinasHeLaBacillus thuringiensisCancercervixparasporinHeLaBacillus thuringiensisMuerte celular inducida por parasporinas y péptidos sintéticos de la parasporina-6 de Bacillus thuringiensis en células de cáncer de cuello uterino y su relación con la vía de adenilato ciclasaTrabajo de grado (Pregrado y/o Especialización)http://purl.org/coar/resource_type/c_7a1fhttp://purl.org/coar/version/c_970fb48d4fbd8a85EspecializadaCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811https://repositorio.uan.edu.co/bitstreams/aecf2c53-aafa-4ee4-9dd3-f99f3c7d2778/download9868ccc48a14c8d591352b6eaf7f6239MD54ORIGINALArticulo Trabajo de Grado - Julian Medina (Version final) .pdfArticulo Trabajo de Grado - Julian Medina (Version final) .pdfTrabajo de gradoapplication/pdf875265https://repositorio.uan.edu.co/bitstreams/be608463-6bed-4141-b165-1ff9263f58dd/download3316ace90afb61cd9eea063c85706b21MD51Formato Autorización de Publicación de Tesis o Trabajo de Grado Julian Guevara.pdfFormato Autorización de Publicación de Tesis o Trabajo de Grado Julian Guevara.pdfAutorización autorapplication/pdf357933https://repositorio.uan.edu.co/bitstreams/973d026d-41fd-47b3-8304-bef7e952809e/download752526714422f0e640c7cf8b2f0a0eafMD52ACTA SUSTENTACION CESAR JULIAN GUEVARA MEDINA PREGRADO BIOQ (1).pdfACTA SUSTENTACION CESAR JULIAN GUEVARA MEDINA PREGRADO BIOQ (1).pdfActaapplication/pdf6193945https://repositorio.uan.edu.co/bitstreams/86310705-5d16-4540-8a78-d32ecbab9910/download1cde6e92208331520e4b9beee01f1092MD53123456789/7108oai:repositorio.uan.edu.co:123456789/71082024-10-09 22:52:43.92https://creativecommons.org/licenses/by-nc-nd/4.0/Acceso abiertoopen.accesshttps://repositorio.uan.edu.coRepositorio Institucional UANalertas.repositorio@uan.edu.co

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