EVALUACIÓN IN VITRO DE LA ACTIVIDAD ANTIMICROBIANA DEL HIDRÓXIDO DE CALCIO EN DOS PREPARACIONES EN PRESENCIA DE ENTEROCOCO FAECALIS

Objective: To assess the antimicrobial effect of two different mixes of calcium hydroxide: with propylenglycol and with chlorhexidine gluconate on Enterococcus faecalis (ATCC29212).Material and methods: Through an experimental in vitro study two agents were tested: pure calcium hydroxide, calcium hydroxide with 13% chlorhexidine gluconate, and calcium hydroxide with propylenglycol. E. faecalis dilutions at 1.5 x 10-7 and 1.5 x 10-8 in soy trypticase were used, to which 100 mg. of each agent were added. They were cultured in Agar Mueller Hinton medium at these intervals: 1, 20, 60, 80, and 120 minutes in direct contact with E. Faecalis strain. They were then incubated at 37° Centigrades, to observe Colony Forming Units (CFU) at 24, 48, and 72 hours. Culture readings were conducted under supervision of one sole operator.Results: Some CFU's were found after one minute of direct contact between microorganism and pure calcium hydroxide, in contrast with other study groups whose CFU readings were zero after one-minute contact. Chlorhexidine gluconate used at 13% showed a better performance; however, results showed no statistically significant differences.

• Autores: Martínez S., Leonor; Acosta L., Hilda Patricia; Duarte M., Martha Lucely
• Fecha de publicación: 2018
• Institución: Universidad Santo Tomás
• Repositorio: Universidad Santo Tomás
• Idioma: spa
• OAI Identifier: oai:repository.usta.edu.co:11634/37171
• Acceso en línea: http://revistas.ustabuca.edu.co/index.php/USTASALUD_ODONTOLOGIA/article/view/1876; http://hdl.handle.net/11634/37171
• License: Derechos de autor 2018 UstaSalud