ACTIVIDAD ANTIMICROBIANA IN VITRO DE UN PROPÓLEO DE SANTANDER SOBRE ENTEROCOCCUS FAECALIS

Objective: to evaluate the antimicrobial activity of an ethanolic propolis extract from Santander province against Enterococcus faecalis.Methods: an experimental In vitro study was made. Starting from pristine propolis collected in the city of Lebrija (Santander), an ethanolic extract was obtained u...

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Autores:
Herrera Sandoval, Laura Viviana
Pisciotti Ortega, Marcos Humberto
Ramos Monsalve, Otoniel
Neira Fuentes, Laura Fernanda
Pinzón Joya, Julio Roberto
Herrera Becerra, Adriana Victoria
Soto Salcedo, José Antonio
Tipo de recurso:
Fecha de publicación:
2012
Institución:
Universidad Santo Tomás
Repositorio:
Repositorio Institucional USTA
Idioma:
spa
OAI Identifier:
oai:repository.usta.edu.co:11634/36996
Acceso en línea:
http://revistas.ustabuca.edu.co/index.php/USTASALUD_ODONTOLOGIA/article/view/1119
http://hdl.handle.net/11634/36996
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Derechos de autor 2018 UstaSalud
id SANTTOMAS2_aaffd6c58a10ecd92de516a733642cf0
oai_identifier_str oai:repository.usta.edu.co:11634/36996
network_acronym_str SANTTOMAS2
network_name_str Repositorio Institucional USTA
repository_id_str
dc.title.spa.fl_str_mv ACTIVIDAD ANTIMICROBIANA IN VITRO DE UN PROPÓLEO DE SANTANDER SOBRE ENTEROCOCCUS FAECALIS
dc.title.alternative.eng.fl_str_mv IN VITRO ANTIMICROBIAL ACTIVITY OF ETANOLIC PROPOLIS EXTRACTS FROM SANTANDER AGAINST ENTEROCOCCUS FAECALIS
title ACTIVIDAD ANTIMICROBIANA IN VITRO DE UN PROPÓLEO DE SANTANDER SOBRE ENTEROCOCCUS FAECALIS
spellingShingle ACTIVIDAD ANTIMICROBIANA IN VITRO DE UN PROPÓLEO DE SANTANDER SOBRE ENTEROCOCCUS FAECALIS
title_short ACTIVIDAD ANTIMICROBIANA IN VITRO DE UN PROPÓLEO DE SANTANDER SOBRE ENTEROCOCCUS FAECALIS
title_full ACTIVIDAD ANTIMICROBIANA IN VITRO DE UN PROPÓLEO DE SANTANDER SOBRE ENTEROCOCCUS FAECALIS
title_fullStr ACTIVIDAD ANTIMICROBIANA IN VITRO DE UN PROPÓLEO DE SANTANDER SOBRE ENTEROCOCCUS FAECALIS
title_full_unstemmed ACTIVIDAD ANTIMICROBIANA IN VITRO DE UN PROPÓLEO DE SANTANDER SOBRE ENTEROCOCCUS FAECALIS
title_sort ACTIVIDAD ANTIMICROBIANA IN VITRO DE UN PROPÓLEO DE SANTANDER SOBRE ENTEROCOCCUS FAECALIS
dc.creator.fl_str_mv Herrera Sandoval, Laura Viviana
Pisciotti Ortega, Marcos Humberto
Ramos Monsalve, Otoniel
Neira Fuentes, Laura Fernanda
Pinzón Joya, Julio Roberto
Herrera Becerra, Adriana Victoria
Soto Salcedo, José Antonio
dc.contributor.author.none.fl_str_mv Herrera Sandoval, Laura Viviana
Pisciotti Ortega, Marcos Humberto
Ramos Monsalve, Otoniel
Neira Fuentes, Laura Fernanda
Pinzón Joya, Julio Roberto
Herrera Becerra, Adriana Victoria
Soto Salcedo, José Antonio
description Objective: to evaluate the antimicrobial activity of an ethanolic propolis extract from Santander province against Enterococcus faecalis.Methods: an experimental In vitro study was made. Starting from pristine propolis collected in the city of Lebrija (Santander), an ethanolic extract was obtained using a Soxhlet apparatus. The antimicrobial activity was measured using the macro dilution tube technique by counting the Colony Forming Units (CFU) per mL after 48 hours. Propolis solutions with concentrations in the range from 100 to 0.19 mg/mL were evaluated. Tubes containing dilutions of the antibiotic norfloxacin and solvent (96% ethanol) were used as reference and control.Results: the propolis solutions within the range from 100 mg/mL to 50 mg/mL inhibited 100% growing of E. faecalis. Propolis extract showed an inhibitory concentration 50 (IC50) of 1.19 mg/mL and an inhibitory concentration 90 (IC90) of 7.92 mg/mL respectively. Norfloxacin on the other hand was more effective with IC50 and IC90 of 0.06 and 2.39 µg/mL respectively. Ethanol showed non-antimicrobial activity at the concentration present within the extracts.Conclusion: with further studies support it would be possible to suggest the use of propolis from Santander as an alternative of irrigation and medication in endodontics because of its powerful effect against E. faecalis considering it is the most frequent pathogen found in persistent infections.
publishDate 2012
dc.date.issued.none.fl_str_mv 2012-07-01
dc.date.accessioned.none.fl_str_mv 2021-09-24T16:23:12Z
dc.date.available.none.fl_str_mv 2021-09-24T16:23:12Z
dc.type.spa.fl_str_mv Artículo revisado por pares
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.drive.none.fl_str_mv info:eu-repo/semantics/article
dc.identifier.none.fl_str_mv http://revistas.ustabuca.edu.co/index.php/USTASALUD_ODONTOLOGIA/article/view/1119
10.15332/us.v11i2.1119
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/11634/36996
url http://revistas.ustabuca.edu.co/index.php/USTASALUD_ODONTOLOGIA/article/view/1119
http://hdl.handle.net/11634/36996
identifier_str_mv 10.15332/us.v11i2.1119
dc.language.iso.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv http://revistas.ustabuca.edu.co/index.php/USTASALUD_ODONTOLOGIA/article/view/1119/918
dc.relation.citationissue.spa.fl_str_mv Ustasalud; Vol. 11 Núm. 2 (2012); 73-78
dc.relation.citationissue.eng.fl_str_mv Ustasalud; Vol. 11 Núm. 2 (2012); 73-78
dc.relation.citationissue.none.fl_str_mv 2590-7875
1692-5106
10.15332/us.v11i2
dc.rights.spa.fl_str_mv Derechos de autor 2018 UstaSalud
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
rights_invalid_str_mv Derechos de autor 2018 UstaSalud
http://purl.org/coar/access_right/c_abf2
dc.format.mimetype.none.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad Santo Tomás Seccional Bucaramanga
institution Universidad Santo Tomás
repository.name.fl_str_mv Repositorio Universidad Santo Tomás
repository.mail.fl_str_mv noreply@usta.edu.co
_version_ 1782026168650694656
spelling Herrera Sandoval, Laura VivianaPisciotti Ortega, Marcos HumbertoRamos Monsalve, OtonielNeira Fuentes, Laura FernandaPinzón Joya, Julio RobertoHerrera Becerra, Adriana VictoriaSoto Salcedo, José Antonio2021-09-24T16:23:12Z2021-09-24T16:23:12Z2012-07-01http://revistas.ustabuca.edu.co/index.php/USTASALUD_ODONTOLOGIA/article/view/111910.15332/us.v11i2.1119http://hdl.handle.net/11634/36996Objective: to evaluate the antimicrobial activity of an ethanolic propolis extract from Santander province against Enterococcus faecalis.Methods: an experimental In vitro study was made. Starting from pristine propolis collected in the city of Lebrija (Santander), an ethanolic extract was obtained using a Soxhlet apparatus. The antimicrobial activity was measured using the macro dilution tube technique by counting the Colony Forming Units (CFU) per mL after 48 hours. Propolis solutions with concentrations in the range from 100 to 0.19 mg/mL were evaluated. Tubes containing dilutions of the antibiotic norfloxacin and solvent (96% ethanol) were used as reference and control.Results: the propolis solutions within the range from 100 mg/mL to 50 mg/mL inhibited 100% growing of E. faecalis. Propolis extract showed an inhibitory concentration 50 (IC50) of 1.19 mg/mL and an inhibitory concentration 90 (IC90) of 7.92 mg/mL respectively. Norfloxacin on the other hand was more effective with IC50 and IC90 of 0.06 and 2.39 µg/mL respectively. Ethanol showed non-antimicrobial activity at the concentration present within the extracts.Conclusion: with further studies support it would be possible to suggest the use of propolis from Santander as an alternative of irrigation and medication in endodontics because of its powerful effect against E. faecalis considering it is the most frequent pathogen found in persistent infections.Objetivo: evaluar la actividad antimicrobiana de un extracto etanólico de propóleo de Santander sobre Enterococcus faecalis.Materiales y métodos: se realizó un estudio experimental In vitro. A partir de un propóleo crudo del municipio de Lebrija (Santander), se obtuvo un extracto etanólico a través del método Soxhlet. La actividad antimicrobiana se determinó con una prueba de macrodilución en tubo y recuento de Unidades Formadoras de Colonias (UFC) por mL luego de 48 horas. Se evaluaron concentraciones de propóleo desde 100 hasta 0,19 mg/mL, como control se incluyeron diluciones del disolvente (etanol 96%) y se usó como medicamento de referencia la norfloxacina.Resultados: se determinó que concentraciones de propóleo de 100 mg/mL y 50 mg/mL inhibieron 100% de crecimiento de E. faecalis. El extracto de propóleo mostró Concentración Inhibitoria 50 (CI50) de 1,19 mg/mL y Concentración Inhibitoria 90 (CI90) de 7,92 mg/mL. Mientras que la norfloxacina fue más efectiva con CI50 y CI90 de 0,06 y 2,39 µg/mL. El etanol no mostró efecto antimicrobiano a las concentraciones presentes en el extracto.Conclusión: con el apoyo de estudios complementarios, es posible proponer al propóleo santandereano como una alternativa de irrigación y medicación en endodoncia, dado el potente efecto frente a E. faecalis siendo este el patógeno endodóntico más frecuente en infecciones persistentes.[Herrera LV, Piscioti MH, Ramos O, Neira LF, Pinzón JR, Herrera AV, Soto JA. Actividad antimicrobiana In Vitro de un propóleo de Santander sobre Enterococcus faecalis. Ustasalud 2012; 11: 73 - 78]application/pdfspaUniversidad Santo Tomás Seccional Bucaramangahttp://revistas.ustabuca.edu.co/index.php/USTASALUD_ODONTOLOGIA/article/view/1119/918Ustasalud; Vol. 11 Núm. 2 (2012); 73-78Ustasalud; Vol. 11 Núm. 2 (2012); 73-782590-78751692-510610.15332/us.v11i2Derechos de autor 2018 UstaSaludhttp://purl.org/coar/access_right/c_abf2ACTIVIDAD ANTIMICROBIANA IN VITRO DE UN PROPÓLEO DE SANTANDER SOBRE ENTEROCOCCUS FAECALISIN VITRO ANTIMICROBIAL ACTIVITY OF ETANOLIC PROPOLIS EXTRACTS FROM SANTANDER AGAINST ENTEROCOCCUS FAECALISArtículo revisado por paresinfo:eu-repo/semantics/articlehttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_2df8fbb111634/36996oai:repository.usta.edu.co:11634/369962023-07-14 16:23:07.549metadata only accessRepositorio Universidad Santo Tomásnoreply@usta.edu.co

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