ACTIVIDAD ANTIMICROBIANA IN VITRO DE UN PROPÓLEO DE SANTANDER SOBRE ENTEROCOCCUS FAECALIS
Objective: to evaluate the antimicrobial activity of an ethanolic propolis extract from Santander province against Enterococcus faecalis.Methods: an experimental In vitro study was made. Starting from pristine propolis collected in the city of Lebrija (Santander), an ethanolic extract was obtained u...
- Autores:
-
Herrera Sandoval, Laura Viviana
Pisciotti Ortega, Marcos Humberto
Ramos Monsalve, Otoniel
Neira Fuentes, Laura Fernanda
Pinzón Joya, Julio Roberto
Herrera Becerra, Adriana Victoria
Soto Salcedo, José Antonio
- Tipo de recurso:
- Fecha de publicación:
- 2012
- Institución:
- Universidad Santo Tomás
- Repositorio:
- Repositorio Institucional USTA
- Idioma:
- spa
- OAI Identifier:
- oai:repository.usta.edu.co:11634/36996
- Acceso en línea:
- http://revistas.ustabuca.edu.co/index.php/USTASALUD_ODONTOLOGIA/article/view/1119
http://hdl.handle.net/11634/36996
- Palabra clave:
- Rights
- License
- Derechos de autor 2018 UstaSalud
| Summary: | Objective: to evaluate the antimicrobial activity of an ethanolic propolis extract from Santander province against Enterococcus faecalis.Methods: an experimental In vitro study was made. Starting from pristine propolis collected in the city of Lebrija (Santander), an ethanolic extract was obtained using a Soxhlet apparatus. The antimicrobial activity was measured using the macro dilution tube technique by counting the Colony Forming Units (CFU) per mL after 48 hours. Propolis solutions with concentrations in the range from 100 to 0.19 mg/mL were evaluated. Tubes containing dilutions of the antibiotic norfloxacin and solvent (96% ethanol) were used as reference and control.Results: the propolis solutions within the range from 100 mg/mL to 50 mg/mL inhibited 100% growing of E. faecalis. Propolis extract showed an inhibitory concentration 50 (IC50) of 1.19 mg/mL and an inhibitory concentration 90 (IC90) of 7.92 mg/mL respectively. Norfloxacin on the other hand was more effective with IC50 and IC90 of 0.06 and 2.39 µg/mL respectively. Ethanol showed non-antimicrobial activity at the concentration present within the extracts.Conclusion: with further studies support it would be possible to suggest the use of propolis from Santander as an alternative of irrigation and medication in endodontics because of its powerful effect against E. faecalis considering it is the most frequent pathogen found in persistent infections. |
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