Células madre y su aplicación biotecnológica
La capacidad autorregenerativa de las células madre es una de sus principales propiedades, la cual ha sido utilizada en procesos de regeneración celular, ya sea para reemplazo o para recuperación celular de tejidos y órganos. Estas células también tienen la habilidad de producir diferentes compuesto...
- Autores:
-
Sánchez Mora, Ruth Mélida
Arévalo Pinzón, Gabriela
Ostos Ortiz, Olga Lucía
- Tipo de recurso:
- Fecha de publicación:
- 2022
- Institución:
- Universidad Santo Tomás
- Repositorio:
- Repositorio Institucional USTA
- Idioma:
- spa
- OAI Identifier:
- oai:repository.usta.edu.co:11634/45876
- Palabra clave:
- Mother cells
hematopoietic stem cells
Regenerative medicine
Células madre
Células madre hematopoyéticas
Medicina regenerativa
- Rights
- License
- Atribución-NoComercial-SinDerivadas 2.5 Colombia
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dc.title.spa.fl_str_mv |
Células madre y su aplicación biotecnológica |
title |
Células madre y su aplicación biotecnológica |
spellingShingle |
Células madre y su aplicación biotecnológica Mother cells hematopoietic stem cells Regenerative medicine Células madre Células madre hematopoyéticas Medicina regenerativa |
title_short |
Células madre y su aplicación biotecnológica |
title_full |
Células madre y su aplicación biotecnológica |
title_fullStr |
Células madre y su aplicación biotecnológica |
title_full_unstemmed |
Células madre y su aplicación biotecnológica |
title_sort |
Células madre y su aplicación biotecnológica |
dc.creator.fl_str_mv |
Sánchez Mora, Ruth Mélida Arévalo Pinzón, Gabriela Ostos Ortiz, Olga Lucía |
dc.contributor.author.none.fl_str_mv |
Sánchez Mora, Ruth Mélida Arévalo Pinzón, Gabriela Ostos Ortiz, Olga Lucía |
dc.contributor.orcid.spa.fl_str_mv |
https://orcid.org/0000-0002-0572-8418 https://orcid.org/0000-0002-5331-5693 https://orcid.org/0000-0002-6477-9872 |
dc.contributor.googlescholar.spa.fl_str_mv |
https://scholar.google.com/citations?hl=es&user=VGWRvDEAAAAJ https://scholar.google.com/citations?hl=es&user=yCBpLUsAAAAJ |
dc.contributor.cvlac.spa.fl_str_mv |
https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000464252 https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000744719 https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000260010 |
dc.contributor.corporatename.spa.fl_str_mv |
Universidad Santo Tomás |
dc.subject.keyword.spa.fl_str_mv |
Mother cells hematopoietic stem cells Regenerative medicine |
topic |
Mother cells hematopoietic stem cells Regenerative medicine Células madre Células madre hematopoyéticas Medicina regenerativa |
dc.subject.lemb.spa.fl_str_mv |
Células madre Células madre hematopoyéticas Medicina regenerativa |
description |
La capacidad autorregenerativa de las células madre es una de sus principales propiedades, la cual ha sido utilizada en procesos de regeneración celular, ya sea para reemplazo o para recuperación celular de tejidos y órganos. Estas células también tienen la habilidad de producir diferentes compuestos farmacológicos y toxicológicos. Así mismo, gracias a sus propiedades antiinflamatorias, antifibróticas regenerativas y antimicrobianas, se sitúan como un recurso biológico interesante. El objetivo de este libro es profundizar en los procesos moleculares y celulares de las células madre y mostrar sus diferentes usos biotecnológicos. |
publishDate |
2022 |
dc.date.accessioned.none.fl_str_mv |
2022-07-16T16:30:32Z |
dc.date.available.none.fl_str_mv |
2022-07-16T16:30:32Z |
dc.date.issued.none.fl_str_mv |
2022 |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2f33 |
dc.type.local.spa.fl_str_mv |
Libro |
dc.type.category.spa.fl_str_mv |
Generación de Nuevo Conocimiento: Libro resultado de investigación |
dc.type.drive.none.fl_str_mv |
info:eu-repo/semantics/book |
dc.identifier.citation.spa.fl_str_mv |
Sánchez, R., Arévalo, G., & Ostos, O. (2022). Células madre y su aplicación biotecnológica. Ediciones USTA. |
dc.identifier.isbn.spa.fl_str_mv |
9789587824995 |
dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/11634/45876 |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.15332/li.lib.2022.00280 |
dc.identifier.reponame.spa.fl_str_mv |
reponame:Repositorio Institucional Universidad Santo Tomás |
dc.identifier.instname.spa.fl_str_mv |
instname:Universidad Santo Tomás |
identifier_str_mv |
Sánchez, R., Arévalo, G., & Ostos, O. (2022). Células madre y su aplicación biotecnológica. Ediciones USTA. 9789587824995 reponame:Repositorio Institucional Universidad Santo Tomás instname:Universidad Santo Tomás |
url |
http://hdl.handle.net/11634/45876 https://doi.org/10.15332/li.lib.2022.00280 |
dc.language.iso.spa.fl_str_mv |
spa |
language |
spa |
dc.relation.uri.spa.fl_str_mv |
https://ediciones.usta.edu.co/index.php/publicaciones/ciencias-sociales/c%C3%A9lulas-madre-y-su-aplicaci%C3%B3n-biotecnol%C3%B3gica-detail |
dc.relation.references.spa.fl_str_mv |
Friedenstein, A. J., Petrakova, K. V., Kurolesova, A. I. y Frolova, G. P. (1968). Heterotopic of bone marrow. Analysis of precursor cells for osteogenic and hematopoietic tissues. Transplantatio, 6(2), 230-247. https://doi.org/10.1097/00007890-196803000-00009 Hao, J., Ma, A., Wang, L., Cao, J., Chen, S., Wang, L., Fu, B., Zhou, J., Pei, X., Zhang, Y., Xiang, P., Hu, S., Li, Q., Zhang, Y., Xia, Y., Zhu, H., Stacey, G., Zhou, Q. y Zhao, T. (2020). General requirements for stem cells. Cell Prolif., 53(12), e12926. https://doi.org/10.1111/cpr.12926 Khan, F. A., Almohazey, D., Alomari, M. y Almofty, S. A. (2018). Isolation, culture, and functional characterization of human embryonic stem cells: current trends and challenges. Stem Cells International. https://doi.org/10.1155/2018/1429351 Mata-Miranda, M., Vázquez-Zapién, G. J. y Sánchez-Monroy, V. (2013). Generalidades y aplicaciones de las células madre. Perinatol. Reprod. Hum., 27(3), 194-199. Sart, S. y Agathos, S. N. (2018). Towards Three-Dimensional Dynamic Regulation and In Situ Characterization of Single Stem Cell Phenotype Using Microfluidics. Molecular Biotechnology, 60(11), 843-861. https://doi.org/10.1007/s12033-018-0113-4 Schmidt, S., Lilienkampf, A. y Bradley, M. (2018). New substrates for stem cell control. Philosophical Transactions of the Royal Society of London Series B, Biological Sciences, 373(1750). https://doi.org/10.1098/rstb.2017.0223 Arbósa, A., Nicolau, F., Quetglas, M., Ramis, J. M., Monjo, M., Muncunill, J., Calvo, J. y Gayà, A. (2013). Obtención de células madre mesenquimales a partir de cordones umbilicales procedentes de un programa altruista de donación de sangre de cordón. Inmunología, 32(1), 3-11. https://doi. org/10.1016/j.inmuno.2012.11.002 Bao, M., Xie, J. y Huck, W. T. S. (2018). Recent advances in engineering the stem cell microniche in 3D. Advanced Science, 5(8), 1800448. https:// doi.org/10.1002/advs.201800448 Bartfeld, S. y Clevers, H. (2017). Stem cell-derived organoids and their application for medical research and patient treatment. Journal of Molecular Medicine, 95(7), 729-738. https://doi.org/10.1007/s00109-017-1531-7 Brizuela, C., Galleguillos, S., Carrión, F., Cabrera, C., Luz, P. e Inostroza, C. (2013). Aislamiento y caracterización de células madre mesenquimales de pulpa y folículo dental humano. Int. J. Morphol., 31(2), 739-746. https:// doi.org/10.4067/S0717-95022013000200063 Centeno, E. G. Z., Cimarosti, H. y Bithell, A. (2018). 2D versus 3D human induced pluripotent stem cell-derived cultures for neurodegenerative disease modelling. Molecular Neurodegeneration, 13(1), 27. https://doi. org/10.1186/s13024-018-0258-4 Donnelly, H., Salmeron-Sanchez, M. y Dalby, M. J. (2018). Designing stem cell niches for differentiation and self-renewal. Journal of the Royal Society Interface, 15(145). https://doi.org/10.1098/rsif.2018.0388 Eto, S., Goto, M., Soga, M., Kaneko, Y., Uehara, Y., Mizuta, H. y Era, T. (2018). Mesenchymal stem cells derived from human iPS cells via mesoderm and neuroepithelium have different features and therapeutic potentials. Plos One, 13(7), e0200790. https://doi.org/10.1371/journal.pone.0200790 Ho, B. X., Pek, N. M. Q. y Soh, B. S. (2018). Disease modeling using 3D organoids derived from human induced pluripotent stem cells. International Journal of Molecular Sciences, 19(4), 936. https://doi.org/10.3390/ijms19040936 Jun, D. Y., Kim, S. Y., Na, J. C., Lee, H. H., Kim, J., Yoon, Y. E, Hong, S. J. y Han, W. K. (2018). Tubular organotypic culture model of human kidney. Plos One, 13(10), e0206447. https://doi.org/10.1371/journal.pone.0206447 Liu, Z., Tang, M., Zhao, J., Chai, R. y Kang, J. (2018). Looking into the Future: Toward Advanced 3D Biomaterials for Stem-Cell-Based Regenerative Medicine. Advanced Materials, 30(17), e1705388. https://doi.org/10.1002/adma.201705388 Mizukami, A. y Swiech, K. (2018). Mesenchymal Stromal Cells: From Discovery to Manufacturing and Commercialization. Stem Cells International. https://doi.org/10.1155/2018/4083921 Osiecki, M. J., Michl, T. D., Kul Babur, B., Kabiri, M., Atkinson, K., Lott, W. B., Griesser H, J. y Doran, M. R. (2015). Packed bed bioreactor for the isolation and expansion of placental-derived mesenchymal stromal cells. Plos One, 10(12), e0144941. https://doi.org/10.1371/journal.pone.0144941 Paim, A., Tessaro, I. C., Cardozo, N. S. M. y Pranke, P. (2018). Mesenchymal stem cell cultivation in electrospun scaffolds: mechanistic modeling for tissue engineering. Journal of Biological Physics, 44(3), 245-271. https://doi.org/10.1007/s10867-018-9482-y Perez-Estenaga, I., Prosper, F. y Pelacho, B. (2018). Allogeneic mesenchymal stem cells and biomaterials: the perfect match for cardiac repair? International Journal of Molecular Sciences, 19(10). https://doi.org/10.3390/ijms19103236 Petry, F., Smith, J. R., Leber, J., Salzig, D., Czermak, P. y Weiss, M. L. (2016). Manufacturing of human umbilical cord mesenchymal stromal cells on microcarriers in a dynamic system for clinical use. Stem Cells Int. https://doi.org/10.1155/2016/4834616 Petry, F., Weidner, T., Czermak, P. y Salzig, D. (2018). Three-dimensional bioreactor technologies for the cocultivation of human mesenchymal stem/ stromal cells and beta cells. Stem Cells Int. https://doi.org/10.1155/2018/2547098 Sane, M. S., Misra, N., Mousa, O. M., Czop, S., Tang, H., Khoo, L. T., Jones, C. D. y Mustafi, S. B. (2018). Cytokines in umbilical cord blood-derived cellular product: a mechanistic insight into bone repair. Regenerative Medicine, 13(8), 881-898. https://doi.org/10.2217/rme-2018-0102 Sart, S. y Agathos, S. N. (2018). Towards three-dimensional dynamic regulation and in situ characterization of single stem cell phenotype using microfluidics. Molecular Biotechnology, 60(11), 843-861. https://doi.org/10.1007/s12033-018-0113-4 Cable, J., Fuchs, E., Weissman, I., Jasper, H., Glass, D., Rando, T. A., Blau, H., Debnath, S., Oliva, A., Park, S., Passegué, E., Kim, C. y Krasnow, M. A. (2020). Adult stem cells and regenerative medicine: a symposium report. Ann N Y Acad Sci., 1462(1), 27-36. https://doi.org/10.1111/nyas.14243 Hawsawi, Y. M., Al-Zahrani, F., Mavromatis, C. H., Baghdadi, M. A., Saggu, S. y Oyouni, A. A. A. (2018). Stem cell applications for treatment of cancer and autoimmune diseases: its promises, obstacles, and future perspectives. Technology in Cancer Research & Treatment, 17. https://doi.org/10.1177/1533033818806910 Labusca, L., Herea, D. D. y Mashayekhi, K. (2018). Stem Cells as Delivery Vehicles for Regenerative Medicine: Challenges and Perspectives. World Journal of Stem Cells, 10(5), 43-56. https://doi.org/10.4252/wjsc.v10.i5.43 Liu, G., David, B. T., Trawczynski, M. y Fessler, R. G. (2020). Advances in Pluripotent Stem Cells: History, Mechanisms, Technologies, and Applications. Stem Cell Rev Rep., 16(1), 3-32. https://doi.org/10.1007/s12015-019-09935-x Mata-Miranda, M., Vázquez-Zapién, G. J. y Sánchez-Monroy, V. (2013). Generalidades y aplicaciones de las células madre. Perinatol. Reprod. Hum., 27(3), 194-199. Ntege, E. H., Sunami, H. y Shimizu, Y. (2020). Advances in regenerative therapy: A review of the literature and future directions. Regen Ther, 14, 136-153. https://doi.org/10.1016/j.reth.2020.01.004 Perez-Estenaga, I., Prosper, F. y Pelacho, B. (2018). Allogeneic Mesenchymal Stem Cells and Biomaterials: The Perfect Match for Cardiac Repair? International Journal of Molecular Sciences, 19(10). https://doi.org/10.3390/ ijms19103236 Serna-Cuéllar, E. y Santamaría-Solís, L. (2013). Protocol of extraction and processing of adult stem cells from abdominal adipose tissue: coordenates of the plastic surgeon in translational researching. Cir. Plást. Iberolatinoam., 39(Supl. 1), s44-s50. https://doi.org/10.4321/S0376-78922013000500012 Serra, M., Brito, C., Correia, C. y Alves, P. M. (2012). Process engineering of human pluripotent stem cells for clinical application. Trends in Biotechnology, 30(6), 350-359. https://doi.org/10.1016/j.tibtech.2012.03.003 Slack, J. M. W. (2018). What is a stem cell? Wiley interdisciplinary Reviews De- velopmental biology, 7(5), e323. https://doi.org/10.1002/wdev.323 Ude, C. C., Miskon, A., Idrus, R. B. H. y Abu Bakar, M. B. (2018). Application of stem cells in tissue engineering for defense medicine. Military Medical Research, 5(1), 7. https://doi.org/10.1186/s40779-018-0154-9 Ullah, I., Subbarao, R. B. y Rho, G. J. (2015). Human mesenchymal stem cells: current trends and future prospective. Bioscience Reports, 35(2). https:// doi.org/10.1042/BSR20150025 Wang, B. X., Kit-Anan, W. y Terracciano, CM. N. (2018). Many cells make life work. Multicellularity in stem cell-based cardiac disease modelling. 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A modified method for isolation of human cardiomyocytes to model cardiac diseases. Journal of Translational Medicine, 16, 288. https://doi. org/10.1186/s12967-018-1649-6 Hassan, S., Simaria, A. S., Varadaraju, H., Gupta, S., Warren, K. y Farid, S. S. (2015). Allogeneic cell therapy bioprocess economics and optimization: downstream processing decisions. Regenerative Medicine, 10(5), 591- 609. https://doi.org/10.2217/rme.15.29 Jossen, V., van den Bos, C., Eibl, R. y Eibl, D. (2018). Manufacturing human mesenchymal stem cells at clinical scale: process and regulatory challenges. Applied Microbiology and Biotechnology, 102(9), 3981-3994. https:// doi.org/10.1007/s00253-018-8912-x Kim, S., Lee, S. K., Kim, H. y Kim, T. M. (2018). Exosomes secreted from induced pluripotent stem cell-derived mesenchymal stem cells accelerate skin cell proliferation. International Journal of Molecular Sciences, 19(10). https://doi.org/10.3390/ijms19103119 Klar, A. S., Zimoch, J. y Biedermann, T. (2017). Skin tissue engineering: application of adipose-derived stem cells. BioMed Research International. https:// doi.org/10.1155/2017/9747010 Lee, J., Cho, Y. S., Jung, H. y Choi, I. (2018). Pharmacological regulation of oxidative stress in stem cells. Oxidative Medicine and Cellular Longevity. https://doi.org/10.1155/2018/4081890 Lin, H., Du, Q., Li, Q., Wang, O., Wang, Z., Sahu, N., Elowsky, C., Liu, K., Zhang, C., Chung, S., Duan, B. y Lei, Y. (2018). A scalable and efficient bioprocess for manufacturing human pluripotent stem cell-de- rived endothelial cells. Stem Cell Reports, 11(2), 454-469. https://doi.org/10.1016/j.stemcr.2018.07.001 Placzek, M. R., Chung, I. M., Macedo, H. M., Ismail, S., Mortera Blanco, T., Lim, M., Jae, M. C., Iliana, F., Yunyi, K., Yeo, D. C. L., Ma Chi, Y. C., Polak, J. M., Panoskaltsis, N. y Mantalaris, A. (2009). Stem cell bioprocessing: fundamentals and principles. Journal of the Royal Society Interface, 6(32), 209-232. https://doi.org/10.1098/rsif.2008.0442 Shyh-Chang, N., Daley, G. Q. y Cantley, L. C. (2013). Stem cell metabolism in tissue development and aging. Development, 140(12), 2535-2547. https://doi.org/10.1242/dev.091777 Silva, M. M., Rodrigues, A. F., Correia, C., Sousa, M. F., Brito, C., Coroadinha, A. S., Serra, M. y Alves, P. M. (2015). Robust expansion of human pluripotent stem cells: integration of bioprocess design with transcriptomic and metabolomic characterization. Stem Cells Translational Medicine, 4(7), 731-742. https://doi.org/10.5966/sctm.2014-0270 Sui, L., Danzl, N., Campbell, S. R., Viola, R., Williams, D., Xing, Y., Wang, Y., Phillips, N., Poffenberger, G., Johannesson, B., Oberholzer, J., Powers, A. C., Leibel, R. L., Chen, X., Sykes, M. y Egli, D. (2018). β-Cell replacement in mice using human type 1 diabetes nuclear transfer embryonic stem cells. Diabetes, 67(1), 26-35. https://doi.org/10.2337/db17-0120 |
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Sánchez Mora, Ruth MélidaArévalo Pinzón, GabrielaOstos Ortiz, Olga Lucíahttps://orcid.org/0000-0002-0572-8418https://orcid.org/0000-0002-5331-5693https://orcid.org/0000-0002-6477-9872https://scholar.google.com/citations?hl=es&user=VGWRvDEAAAAJhttps://scholar.google.com/citations?hl=es&user=yCBpLUsAAAAJhttps://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000464252https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000744719https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000260010Universidad Santo Tomás2022-07-16T16:30:32Z2022-07-16T16:30:32Z2022Sánchez, R., Arévalo, G., & Ostos, O. (2022). Células madre y su aplicación biotecnológica. Ediciones USTA.9789587824995http://hdl.handle.net/11634/45876https://doi.org/10.15332/li.lib.2022.00280reponame:Repositorio Institucional Universidad Santo Tomásinstname:Universidad Santo TomásLa capacidad autorregenerativa de las células madre es una de sus principales propiedades, la cual ha sido utilizada en procesos de regeneración celular, ya sea para reemplazo o para recuperación celular de tejidos y órganos. Estas células también tienen la habilidad de producir diferentes compuestos farmacológicos y toxicológicos. Así mismo, gracias a sus propiedades antiinflamatorias, antifibróticas regenerativas y antimicrobianas, se sitúan como un recurso biológico interesante. El objetivo de este libro es profundizar en los procesos moleculares y celulares de las células madre y mostrar sus diferentes usos biotecnológicos.1-127spaUniversidad Santo TomásProducción Editorialhttps://ediciones.usta.edu.co/index.php/publicaciones/ciencias-sociales/c%C3%A9lulas-madre-y-su-aplicaci%C3%B3n-biotecnol%C3%B3gica-detailFriedenstein, A. J., Petrakova, K. V., Kurolesova, A. I. y Frolova, G. P. (1968). Heterotopic of bone marrow. Analysis of precursor cells for osteogenic and hematopoietic tissues. Transplantatio, 6(2), 230-247. https://doi.org/10.1097/00007890-196803000-00009Hao, J., Ma, A., Wang, L., Cao, J., Chen, S., Wang, L., Fu, B., Zhou, J., Pei, X., Zhang, Y., Xiang, P., Hu, S., Li, Q., Zhang, Y., Xia, Y., Zhu, H., Stacey, G., Zhou, Q. y Zhao, T. (2020). General requirements for stem cells. Cell Prolif., 53(12), e12926. https://doi.org/10.1111/cpr.12926Khan, F. A., Almohazey, D., Alomari, M. y Almofty, S. A. (2018). 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Diabetes, 67(1), 26-35. https://doi.org/10.2337/db17-0120Atribución-NoComercial-SinDerivadas 2.5 Colombiahttp://creativecommons.org/licenses/by-nc-nd/2.5/co/Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2Células madre y su aplicación biotecnológicaMother cellshematopoietic stem cellsRegenerative medicineCélulas madreCélulas madre hematopoyéticasMedicina regenerativaLibroGeneración de Nuevo Conocimiento: Libro resultado de investigacióninfo:eu-repo/semantics/bookhttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_2f33CRAI-USTA BogotáORIGINALObracompleta.Coleccioncienciasdelasalud.2022Sanchezruth.pdfObracompleta.Coleccioncienciasdelasalud.2022Sanchezruth.pdfapplication/pdf7965710https://repository.usta.edu.co/bitstream/11634/45876/1/Obracompleta.Coleccioncienciasdelasalud.2022Sanchezruth.pdfee59f10768ef56a396eed7b33410a72aMD51open accessCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811https://repository.usta.edu.co/bitstream/11634/45876/2/license_rdf217700a34da79ed616c2feb68d4c5e06MD52open accessLICENSElicense.txtlicense.txttext/plain; charset=utf-8807https://repository.usta.edu.co/bitstream/11634/45876/3/license.txtaedeaf396fcd827b537c73d23464fc27MD53open accessTHUMBNAILObracompleta.Coleccioncienciasdelasalud.2022Sanchezruth.pdf.jpgObracompleta.Coleccioncienciasdelasalud.2022Sanchezruth.pdf.jpgIM Thumbnailimage/jpeg3451https://repository.usta.edu.co/bitstream/11634/45876/4/Obracompleta.Coleccioncienciasdelasalud.2022Sanchezruth.pdf.jpg872cc7dc1a7b374bd4bfcf70ac23c2d9MD54open access11634/45876oai:repository.usta.edu.co:11634/458762022-12-05 09:00:50.636open accessRepositorio Universidad Santo Tomásrepositorio@usantotomas.edu.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 |