Evaluación de la microfiltracion microbiana de Enterococcus faecalis y Cándida albicans en dientes tratados endodonticamente obturados con Bioroot y Ahplus
Objetivo: Evaluar la microfiltración microbiana del cemento Bioroot en comparación con el AHPlus. Materiales y Método: Se utilizaron 30 dientes uniradiculares los cuales fueron instrumentados con limas Wave One Gold, irrigando con hipoclorito de sodio al 5,25% entre cada instrumento. Se realizó irri...
- Autores:
-
Díaz Marín, Angélica María
Plazas Ortegón, Lina María
Murcia Moreno, Andrea Yineth
- Tipo de recurso:
- Trabajo de grado de pregrado
- Fecha de publicación:
- 2020
- Institución:
- Universidad Santo Tomás
- Repositorio:
- Repositorio Institucional USTA
- Idioma:
- spa
- OAI Identifier:
- oai:repository.usta.edu.co:11634/28017
- Acceso en línea:
- http://hdl.handle.net/11634/28017
- Palabra clave:
- Microfiltration
Endodontics
Endodontic sealer
BioRoot
AH plus
Endodoncia
Adhesivos dentales
Canal radicular - Tratamiento
Cultivos bacteriológicos
Microfiltración
Endodoncia
Cemento obturador
Bioroot
AH plus
- Rights
- openAccess
- License
- CC0 1.0 Universal
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| dc.title.spa.fl_str_mv |
Evaluación de la microfiltracion microbiana de Enterococcus faecalis y Cándida albicans en dientes tratados endodonticamente obturados con Bioroot y Ahplus |
| title |
Evaluación de la microfiltracion microbiana de Enterococcus faecalis y Cándida albicans en dientes tratados endodonticamente obturados con Bioroot y Ahplus |
| spellingShingle |
Evaluación de la microfiltracion microbiana de Enterococcus faecalis y Cándida albicans en dientes tratados endodonticamente obturados con Bioroot y Ahplus Microfiltration Endodontics Endodontic sealer BioRoot AH plus Endodoncia Adhesivos dentales Canal radicular - Tratamiento Cultivos bacteriológicos Microfiltración Endodoncia Cemento obturador Bioroot AH plus |
| title_short |
Evaluación de la microfiltracion microbiana de Enterococcus faecalis y Cándida albicans en dientes tratados endodonticamente obturados con Bioroot y Ahplus |
| title_full |
Evaluación de la microfiltracion microbiana de Enterococcus faecalis y Cándida albicans en dientes tratados endodonticamente obturados con Bioroot y Ahplus |
| title_fullStr |
Evaluación de la microfiltracion microbiana de Enterococcus faecalis y Cándida albicans en dientes tratados endodonticamente obturados con Bioroot y Ahplus |
| title_full_unstemmed |
Evaluación de la microfiltracion microbiana de Enterococcus faecalis y Cándida albicans en dientes tratados endodonticamente obturados con Bioroot y Ahplus |
| title_sort |
Evaluación de la microfiltracion microbiana de Enterococcus faecalis y Cándida albicans en dientes tratados endodonticamente obturados con Bioroot y Ahplus |
| dc.creator.fl_str_mv |
Díaz Marín, Angélica María Plazas Ortegón, Lina María Murcia Moreno, Andrea Yineth |
| dc.contributor.advisor.spa.fl_str_mv |
Lancheros Bonilla, Sonia |
| dc.contributor.advisor.none.fl_str_mv |
Jiménez, Mauricio |
| dc.contributor.author.spa.fl_str_mv |
Díaz Marín, Angélica María Plazas Ortegón, Lina María Murcia Moreno, Andrea Yineth |
| dc.subject.keyword.spa.fl_str_mv |
Microfiltration Endodontics Endodontic sealer BioRoot AH plus |
| topic |
Microfiltration Endodontics Endodontic sealer BioRoot AH plus Endodoncia Adhesivos dentales Canal radicular - Tratamiento Cultivos bacteriológicos Microfiltración Endodoncia Cemento obturador Bioroot AH plus |
| dc.subject.lemb.spa.fl_str_mv |
Endodoncia Adhesivos dentales Canal radicular - Tratamiento Cultivos bacteriológicos |
| dc.subject.proposal.spa.fl_str_mv |
Microfiltración Endodoncia Cemento obturador Bioroot AH plus |
| description |
Objetivo: Evaluar la microfiltración microbiana del cemento Bioroot en comparación con el AHPlus. Materiales y Método: Se utilizaron 30 dientes uniradiculares los cuales fueron instrumentados con limas Wave One Gold, irrigando con hipoclorito de sodio al 5,25% entre cada instrumento. Se realizó irrigación final con hipoclorito de sodio y EDTA al 17%. Los dientes fueron divididos aleatoriamente en dos grupos experimentales Grupo1 Bioroot condensación lateral y Grupo 2 AH Plus condensación lateral. Los dientes fueron esterilizados a vapor, se realizó montaje de doble cámara y se inoculó con suspensión microbiana de 1x10 5 con de E Faecalis y 1x10 5 C albicans, cada 24 horas durante 60 días. Resultados: Los resultados de este estudio muestran que los valores extremos son aproximadamente cero, lo que confirma que no existen diferencias significativas entre los dos sellantes, dando como resultado un nivel de confianza del 99% con un valor p- valor de 0.7053. Conclusiones: no hay diferencia estadísticamente significativa en la microfiltración de los dos cementos estudiados. |
| publishDate |
2020 |
| dc.date.accessioned.spa.fl_str_mv |
2020-07-13T21:46:52Z |
| dc.date.available.spa.fl_str_mv |
2020-07-13T21:46:52Z |
| dc.date.issued.spa.fl_str_mv |
2020-06-06 |
| dc.type.local.spa.fl_str_mv |
Trabajo de grado |
| dc.type.version.none.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
| dc.type.category.spa.fl_str_mv |
Formación de Recurso Humano para la Ctel: Trabajo de grado de Especialización |
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http://purl.org/coar/resource_type/c_7a1f |
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info:eu-repo/semantics/bachelorThesis |
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http://purl.org/coar/resource_type/c_7a1f |
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acceptedVersion |
| dc.identifier.citation.spa.fl_str_mv |
Díaz Marín, A. M., Plazas Ortegón, L. M. y Murcia Moreno, A Y. (2020). Evaluación de la microfiltracion microbiana de Enterococcus faecalis y Cándida albicans en dientes tratados endodonticamente obturados con Bioroot y Ahplus [Tesis de posgrado]. Universidad Santo Tomás, Bucaramanga, Colombia |
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http://hdl.handle.net/11634/28017 |
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reponame:Repositorio Institucional Universidad Santo Tomás |
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instname:Universidad Santo Tomás |
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repourl:https://repository.usta.edu.co |
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Díaz Marín, A. M., Plazas Ortegón, L. M. y Murcia Moreno, A Y. (2020). Evaluación de la microfiltracion microbiana de Enterococcus faecalis y Cándida albicans en dientes tratados endodonticamente obturados con Bioroot y Ahplus [Tesis de posgrado]. Universidad Santo Tomás, Bucaramanga, Colombia reponame:Repositorio Institucional Universidad Santo Tomás instname:Universidad Santo Tomás repourl:https://repository.usta.edu.co |
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http://hdl.handle.net/11634/28017 |
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spa |
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spa |
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INGLE JI. Root canal obturation. J Am Dent Assoc. 1956;53(1):47–55. Sridhar LP, Moses J, Rangeeth BN, Sivakumar S. Comparative evaluation of the marginal sealing ability of two commercially available pit and fissure sealants. J Clin Diagnostic Res. 2016;10(9):ZC01–4. Rangel Cobos Octavio Manuel, Luna Lara Carlos Alberto, Tellez Garza Alejandro LFMT. Obturación del sistema de conductos radiculares. Una revisión de la literatura. Revista ADM. 2018;269–72. Silva-Herzog Daniel, García Lazcano Claudia Cecilia, Rodríguez Arreguín Mario Hugo GAAM. Invasión por Candida albicans y Enterococcus faecalis en dentina humana. Revista Nacional de Odontológica Año 3- Vol VIII. 2011; Aktemur Türker S, Uzunoğlu E, Purali N. Evaluation of dentinal tubule penetration depth and push-out bond strength of AH 26, BioRoot RCS, and MTA Plus root canal sealers in presence or absence of smear layer. J Dent Res Dent Clin Dent Prospects. 2018;12(4):294–8. Mamootil K, Messer HH. Penetration of dentinal tubules by endodontic sealer cements in extracted teeth and in vivo. Int Endod J. 2007;40(11):873–81. Guerrero F, Berástegui E, Aspiazu K. Porosity analysis of mineral trioxide aggregate Fillapex and BioRoot cements for use in endodontics using microcomputed tomography. J Conserv Dent. 2018;21(5):491–4. Alsubait S, Albader S, Alajlan N, Alkhunaini N, Niazy A, Almahdy A. Comparison of the antibacterial activity of calcium silicate- and epoxy resin-based endodontic sealers against Enterococcus faecalis biofilms: a confocal laser-scanning microscopy analysis. Odontology [Internet]. 2019;107(4):513–20. Available from: http://dx.doi.org/10.1007/s10266-019-00425-7 Torabinejad Mahmoud WR. Endodoncia. Principios y práctica 4th Edition. In: Elsevier 2009. p. 496. de Chevigny C, Dao TT, Basrani BR, Marquis V, Farzaneh M, Abitbol S, et al. Treatment Outcome in Endodontics: The Toronto Study-Phases 3 and 4: Orthograde Retreatment. J Endod. 2008;34(2):131–7. Nair PNR. Pathogenesis of apical periodontitis and the causes of endodontic failures. Vol. 15, Critical Reviews in Oral Biology and Medicine. 2004. 348–381 p. Bernal-Treviño A, González-Amaro AM, Méndez González V, Pozos-Guillen A. Frecuencia de Candida en conductos radiculares de dientes con infección endodóntica primaria y persistente. Rev Iberoam Micol. 2018;35(2):78–82. Collado-González M, García-Bernal D, Oñate-Sánchez RE, Ortolani-Seltenerich PS, Lozano A, Forner L, et al. Biocompatibility of three new calcium silicate-based endodontic sealers on human periodontal ligament stem cells. Int Endod J. 2017;50(9):875–84. Camps J, Jeanneau C, El Ayachi I, Laurent P, About I. Bioactivity of a Calcium Silicate-based Endodontic Cement (BioRoot RCS): Interactions with Human Periodontal Ligament Cells In Vitro. J Endod [Internet]. 2015;41(9):1469–73. Available from: http://dx.doi.org/10.1016/j.joen.2015.04.011 Kakoura F, Pantelidou O. Retreatability of root canals filled with Gutta percha and a novel bioceramic sealer: A scanning electron microscopy study. J Conserv Dent. 2018;21(6):632–6. Xuereb M, Vella P, Damidot D, Sammut C V, Camilleri J. In situ assessment of the setting of tricalcium silicate-based sealers using a dentin pressure model. J Endod. 2015 Jan;41(1):111–24. Langlais RP, Rodriguez IE, Maselle I. Principles of radiographic selection and interpretation. Dent Clin North Am. 1994 Jan;38(1):1–12. Caicedo R, von Fraunhofer JA. The properties of endodontic sealer cements. J Endod. 1988 Nov;14(11):527–34. Figueiredo J, Braga C, Kappel E, Charão E, Mildner M CR. Comparative analysis of the quality of root fillings using taper 0.04 and 0.06 master cones. Brazilian Endod J. 2001;5(1–2):23–31. Kazemi RB, Safavi KE, Spångberg LS. Dimensional changes of endodontic sealers. Oral Surg Oral Med Oral Pathol. 1993 Dec;76(6):766–71. Branstetter J, von Fraunhofer JA. The physical properties and sealing action of endodontic sealer cements: a review of the literature. J Endod. 1982 Jul;8(7):312–6. Jainaen A, Palamara JEA, Messer HH. The effect of a resin-based sealer cement on micropunch shear strength of dentin. J Endod. 2008 Oct;34(10):1215–8. Miletić I, Jukić S, Anić I, Željezić D, Garaj-Vrhovac V, Osmak M. Examination of cytotoxicity and mutagenicity of AH26 and AH Plus sealers. Int Endod J. 2003;36(5):330–5. Leyhausen G, Heil J, Reifferscheid G, Waldmann P, Geurtsen W. Genotoxicity and cytotoxicity of the epoxy resin-based root canal sealer AH plus. J Endod. 1999 Feb;25(2):109–13. Schweikl H, Schmalz G, Stimmelmayr H, Bey B. Mutagenicity of AH26 in an in vitro mammalian cell mutation assay. J Endod. 1995 Aug;21(8):407–10 García Ávila G, García Aranda RL, Perea Mejía LM. Comparación in vitro de la actividad antimicrobiana de AhPlus, RSA y Ledermix contra Enterococcus faecalis . Vol. 17, Revista odontológica mexicana . scielomx ; 2013. p. 156–60 Arikatla SK, Chalasani U, Mandava J, Yelisela RK. Interfacial adaptation and penetration depth of bioceramic endodontic sealers. J Conserv Dent. 2018;21(4):373–7. Guneser MB, Akman M, Kolcu İB, Eldeniz AU. Fracture resistance of roots obturated with a novel calcium silicate-based endodontic sealer (BioRoot RCS). J Adhes Sci Technol [Internet]. 2016 Nov 16;30(22):2420–8. Available from: https://doi.org/10.1080/01694243.2016.1184403 Viapiana R, Moinzadeh AT, Camilleri L, Wesselink PR, Tanomaru Filho M, Camilleri J. Porosity and sealing ability of root fillings with gutta-percha and BioRoot RCS or AH Plus sealers. Evaluation by three ex vivo methods. Int Endod J. 2016;49(8):774–82. Urban K, Neuhaus J, Donnermeyer D, Schäfer E, Dammaschke T. Solubility and pH Value of 3 Different Root Canal Sealers: A Long-term Investigation. J Endod. 2018 Nov;44(11):1736–40. Vouzara T, Dimosiari G, Koulaouzidou EA, Economides N. Cytotoxicity of a New Calcium Silicate Endodontic Sealer. J Endod. 2018 May;44(5):849–52. Kebudi Benezra M, Schembri Wismayer P, Camilleri J. Interfacial Characteristics and Cytocompatibility of Hydraulic Sealer Cements. J Endod. 2018 Jun;44(6):1007–17. Khalil I, Naaman A, Camilleri J. Properties of Tricalcium Silicate Sealers. J Endod. 2016 Oct;42(10):1529–35. Prüllage R-K, Urban K, Schäfer E, Dammaschke T. Material Properties of a Tricalcium Silicate-containing, a Mineral Trioxide Aggregate-containing, and an Epoxy Resin-based Root Canal Sealer. J Endod. 2016 Dec;42(12):1784–8. Mente J, Ferk S, Dreyhaupt J, Deckert A, Legner M, Staehle HJ. Assessment of different dyes used in leakage studies. Clin Oral Investig. 2010 Jun;14(3):331–8 DOW PR, INGLE JI. Isotope determination of root canal failure. Oral Surg Oral Med Oral Pathol. 1955 Oct;8(10):1100–4. Wu M, Wesselink paul. Endodontic leakage studies reconsidered. Part I. Methodology, application and relevance. Int Endod J. 1993 Feb 1;26:37–43. Aminsobhani M, Ghorbanzadeh A, Bolhari B, Shokouhinejad N, Ghabraei S, Assadian H, et al. Coronal microleakage in root canals obturated with lateral compaction, warm vertical compaction and guttaflow system. Iran Endod J. 2010;5(2):83–7. Timpawat S, Amornchat C, Trisuwan WR. Bacterial coronal leakage after obturation with three root canal sealers. J Endod. 2001;27(1):36–9. Torabinejad M, Ung B, Kettering JD. In vitro bacterial penetration of coronally unsealed endodontically treated teeth. J Endod. 1990;16(12):566–9. Monardes Cortés H, Abarca Reveco J, Castro Hurtado P. Microfiltración Apical de Dos Cementos Selladores: Un Estudio in vitro. Int J Odontostomatol. 2014;8(3):393–8. Stuart CH, Schwartz SA, Beeson TJ, Owatz CB. Enterococcus faecalis: Its role in root canal treatment failure and current concepts in retreatment. J Endod. 2006;32(2):93–8 Paradella TC, Koga-Ito CY, Jorge AOC. Enterococcus faecalis: considerações clínicas e microbiológicas. Rev odontol UNESP. 2007;36(2):163–8. Al-Haddad A, Aziz ZACA. Bioceramic-Based Root Canal Sealers: A Review. Int J Biomater. 2016;2016. Sundqvist G. Associations between microbial species in dental root canal infections. Oral Microbiol Immunol. 1992;7(5):257–62. Pourhajibagher M, Ghorbanzadeh R, Parker S, Chiniforush N, Bahador A. The evaluation of cultivable microbiota profile in patients with secondary endodontic infection before and after photo-activated disinfection. Photodiagnosis Photodyn Ther [Internet]. 2017;18:198–203. Available from: http://dx.doi.org/10.1016/j.pdpdt.2017.02.013 Leonard JE, Gutmann JL, Guo IY. Apical and coronal seal of roots obturated with a dentine bonding agent and resin. Int Endod J. 1996;29(2):76–83. Veríssimo DM, do Vale MS. Methodologies for assessment of apical and coronal leakage of endodontic filling materials: a critical review. J Oral Sci. 2006;48(3):93–8. Yanpiset K, Banomyong D, Chotvorrarak K, Srisatjaluk RL. Bacterial leakage and micro-computed tomography evaluation in round-shaped canals obturated with bioceramic cone and sealer using matched single cone technique. Restor Dent Endod. 2018;43(3):1–12 Bouillaguet S, Shaw L, Barthelemy J, Krejci I, Wataha JC. Long-term sealing ability of Pulp Canal Sealer, AH-Plus, GuttaFlow and Epiphany. Int Endod J. 2008;41(3):219–26. Mobarak A, Moussa S, Zaazou A, Abdelfattah H. Comparison of Bacterial Coronal Leakage Between Different Obturation Materials (an in Vitro Study). Alexandria Dent J. 2015;40(1):1–7. Surya Raghavendra S, Jadhav GR, Gathani KM, Kotadia P. Bioceramics in Endodontics – a Review. J Istanbul Univ Fac Dent. 2017;51(0):128–37. Reszka P, Nowicka A, Lipski M, Dura W, Droździk A, Woźniak K. A Comparative Chemical Study of Calcium Silicate-Containing and Epoxy Resin-Based Root Canal Sealers. Biomed Res Int. 2016;2016. Atmeh AR, Chong EZ, Richard G, Festy F, Watson TF. Dentin-cement interfacial interaction: Calcium silicates and polyalkenoates. J Dent Res. 2012;91(5):454–9. Du T, Wang Z, Shen Y, Ma J, Cao Y, Haapasalo M. Effect of long-term exposure to endodontic disinfecting solutions on young and old Enterococcus faecalis biofilms in dentin canals. J Endod [Internet]. 2014;40(4):509–14. Available from: http://dx.doi.org/10.1016/j.joen.2013.11.026 Chivatxaranukul P, Dashper SG, Messer HH. Dentinal tubule invasion and adherence by Enterococcus faecalis. Int Endod J. 2008;41(10):873–82. |
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Lancheros Bonilla, SoniaJiménez, MauricioDíaz Marín, Angélica MaríaPlazas Ortegón, Lina MaríaMurcia Moreno, Andrea Yineth2020-07-13T21:46:52Z2020-07-13T21:46:52Z2020-06-06Díaz Marín, A. M., Plazas Ortegón, L. M. y Murcia Moreno, A Y. (2020). Evaluación de la microfiltracion microbiana de Enterococcus faecalis y Cándida albicans en dientes tratados endodonticamente obturados con Bioroot y Ahplus [Tesis de posgrado]. Universidad Santo Tomás, Bucaramanga, Colombiahttp://hdl.handle.net/11634/28017reponame:Repositorio Institucional Universidad Santo Tomásinstname:Universidad Santo Tomásrepourl:https://repository.usta.edu.coObjetivo: Evaluar la microfiltración microbiana del cemento Bioroot en comparación con el AHPlus. Materiales y Método: Se utilizaron 30 dientes uniradiculares los cuales fueron instrumentados con limas Wave One Gold, irrigando con hipoclorito de sodio al 5,25% entre cada instrumento. Se realizó irrigación final con hipoclorito de sodio y EDTA al 17%. Los dientes fueron divididos aleatoriamente en dos grupos experimentales Grupo1 Bioroot condensación lateral y Grupo 2 AH Plus condensación lateral. Los dientes fueron esterilizados a vapor, se realizó montaje de doble cámara y se inoculó con suspensión microbiana de 1x10 5 con de E Faecalis y 1x10 5 C albicans, cada 24 horas durante 60 días. Resultados: Los resultados de este estudio muestran que los valores extremos son aproximadamente cero, lo que confirma que no existen diferencias significativas entre los dos sellantes, dando como resultado un nivel de confianza del 99% con un valor p- valor de 0.7053. Conclusiones: no hay diferencia estadísticamente significativa en la microfiltración de los dos cementos estudiados.Objective: To evaluate the microbial microfiltration of Bioroot cement compared to AHPlus. Materials and Method: 34 uniradicular teeth were used, each one were instrumented with Wave One Gold files, irrigating with 5.25% sodium hypochlorite between each instrument. Final irrigation was performed with sodium hypochlorite and 17% EDTA. The teeth were randomly divided into two experimental groups Group1 Bioroot lateral condensation and Group 2 AH Plus lateral condensation. The teeth were steam sterilized, a double chamber assembly was made and inoculated with 1x105 microbial suspension with E Faecalis and 1x105 C albicans, every 24 hours for 60 days. Results: The results of this study shows that the extreme values are approximately zero, confirming that there are no significant differences between the two sealants, resulting in a 99% confidence level with a p-value of 0.7053. Conclusions: there is no statistically significant difference in the microfiltration of the two cements studied.Especialista en Endodonciahttp://www.ustabuca.edu.co/ustabmanga/presentacionEspecializaciónapplication/pdfspaUniversidad Santo TomásEspecialización EndodonciaFacultad de OdontologíaCC0 1.0 Universalhttp://creativecommons.org/publicdomain/zero/1.0/Abierto (Texto Completo)info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Evaluación de la microfiltracion microbiana de Enterococcus faecalis y Cándida albicans en dientes tratados endodonticamente obturados con Bioroot y AhplusMicrofiltrationEndodonticsEndodontic sealerBioRootAH plusEndodonciaAdhesivos dentalesCanal radicular - TratamientoCultivos bacteriológicosMicrofiltraciónEndodonciaCemento obturadorBiorootAH plusTrabajo de gradoinfo:eu-repo/semantics/acceptedVersionFormación de Recurso Humano para la Ctel: Trabajo de grado de Especializaciónhttp://purl.org/coar/resource_type/c_7a1finfo:eu-repo/semantics/bachelorThesisCRAI-USTA BucaramangaINGLE JI. Root canal obturation. J Am Dent Assoc. 1956;53(1):47–55.Sridhar LP, Moses J, Rangeeth BN, Sivakumar S. Comparative evaluation of the marginal sealing ability of two commercially available pit and fissure sealants. J Clin Diagnostic Res. 2016;10(9):ZC01–4.Rangel Cobos Octavio Manuel, Luna Lara Carlos Alberto, Tellez Garza Alejandro LFMT. Obturación del sistema de conductos radiculares. Una revisión de la literatura. Revista ADM. 2018;269–72.Silva-Herzog Daniel, García Lazcano Claudia Cecilia, Rodríguez Arreguín Mario Hugo GAAM. Invasión por Candida albicans y Enterococcus faecalis en dentina humana. Revista Nacional de Odontológica Año 3- Vol VIII. 2011;Aktemur Türker S, Uzunoğlu E, Purali N. Evaluation of dentinal tubule penetration depth and push-out bond strength of AH 26, BioRoot RCS, and MTA Plus root canal sealers in presence or absence of smear layer. J Dent Res Dent Clin Dent Prospects. 2018;12(4):294–8.Mamootil K, Messer HH. 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