Inducción In vitro de la reacción acrosómica con heparina en semen congelado de toros brahman y gyr

The aim of this study was to value the in vitro induction by heparin of acrosome reaction in frozen semen from Brahman and Gyr bulls, as a method to estimate frozen semen fertility. Seven sexually mature Zebu bulls (4 Brahman and 3 Gyr bulls) were used in this study. In a post-thawing seminal assessment the progressive rapid individual motility (MPIR), primary abnormalities (AS), secondary abnormalities (AP), total abnormalities (AT) and percentage of acrosome reaction induction (RA) were studied. Acrosome reactions were determined on a glass microscopic slide whith eosine-nigrosine stain and examining under 100X magnification, using optical microscopy. There was no difference between the seminal doses for both races in the MOTP, spermatic morphology and RA (p<0.05), however AP made an exception, indicating that the Brahman type presented a lower percentage of defects than the Gyr type (p>0.05). No correlation was observed between semen quality assessment (MPIR, AS, AP, AT and RA) and the non-return rate of the bulls (p<0.05). The results indicate that RA technique can be used as complementary test in the seminal routine assessment of frozen bovine semen; however, alone it is no effective in predicting the fertilizing potential of cryopreserved semen.

• Autores: Montes, José; Torres, María; Rugeles, Clara; Almanza, Roberto; Guimarães, José
• Tipo de recurso: Article of journal
• Fecha de publicación: 2012
• Institución: Universidad de Ciencias Aplicadas y Ambientales U.D.C.A
• Repositorio: Repositorio Institucional UDCA
• Idioma: spa
• OAI Identifier: oai:repository.udca.edu.co:11158/1839
• Acceso en línea: https://revistas.udca.edu.co/index.php/ruadc/article/view/844
• Palabra clave: Acrosoma; Inductor; Capacitación; Fertilidad; Toro; Acrosoma; Heparina
• Rights: openAccess
• License: Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales