Quantification of two isomeric flavones in rat colon tissue using reverse phase high performance liquid chromatography

Background: Antineoplastic activity has been previously shown for two isomeric flavones, 5,7-dihydroxy-3,6,8-trimethoxy flavone (flavone A) and 3,5-dihydroxy-6,7,8-trimethoxy flavone (flavone B), against colon cancer cell lines (Thomas et al. in PLoS ONE 7:e39806, 5). Here, we present modified methods for the extraction and quantification of flavones A and B in rat colon tissue after intravenous dosing via high performance liquid chromatography, from the originally described procedure for extraction and quantification in rat plasma (Whitted et al. in J Chromatogr B Analyt Technol Biomed Life Sci 1001:150-155, 7). Results: Modifications included tissue homogenization (1 g tissue: 2 mL water), filtration of the supernatant with a PVDF membrane, and the use of only one calibration curve to determine the concentration of each flavone in colon tissue. Good separation was achieved and representative equations were linear with r 2 ≥ 0.99 for both flavones. Precision and accuracy for flavone A ranged from 0.88-24.03 and 109-116%. Precision and accuracy for flavone B ranged from 1.62-33.56 and 98-113%. Concentrations of 1639 ± 601 ng/g flavone A and 5975 ± 2480 ng/g of flavone B were detected in rat colon tissue 6 h post dosing. Conclusions: Modifications to the extraction methods for flavone A and flavone B from rat colon tissue had good separation, precision, and accuracy

• Autores: Whitted, Crystal L.; Palau, Victoria E.; Torrenegra, Rubén D.; Rodrigez, Oscar E.; Harirforoosh, Sam
• Tipo de recurso: Article of journal
• Fecha de publicación: 2017
• Institución: Universidad de Ciencias Aplicadas y Ambientales U.D.C.A
• Repositorio: Repositorio Institucional UDCA
• Idioma: eng
• OAI Identifier: oai:repository.udca.edu.co:11158/2412
• Acceso en línea: https://bmcresnotes.biomedcentral.com/track/pdf/10.1186/s13104-016-2358-y
• Palabra clave: Helichrysum; Aceites Volátiles; Cromatografía Líquida de Alta Presión; Calibración; Línea Celular Tumoral; Cromatografía de Fase Inversa; Flavonas; Helichrysum; Olis volatile; Chromatography, High Pressure Liquid; Calibration; Cell Line, Tumor; Chromatography, Reverse-Phase; Flavones
• Rights: openAccess
• License: Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales