Evaluación de la actividad crioprotectora de dos almidones de yuca sobre células mononucleadas de sangre de cordón umbilical
This research determined the possibility to change the hydroxyethyl starch (HES), conventional crioprotector, for two sorts of mandioca starches made by national industry. The study verified the influence of the mandioca starches on the hidrogenions amount in blood and confirmed the estability of st...
- Autores:
-
Ortiz Trujillo, Bibiana
Ospino, Benjamín
Trujillo, Miosotis
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2007
- Institución:
- Universidad de Ciencias Aplicadas y Ambientales U.D.C.A
- Repositorio:
- Repositorio Institucional UDCA
- Idioma:
- spa
- OAI Identifier:
- oai:repository.udca.edu.co:11158/2460
- Acceso en línea:
- https://revistas.udca.edu.co/index.php/ruadc/article/view/591
- Palabra clave:
- Crioprotección
Hidroxietil almidón
Almidón de yuca
Reológicas
Mandioca
Almidón
Células
Criopreservación
- Rights
- openAccess
- License
- Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales
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Evaluación de la actividad crioprotectora de dos almidones de yuca sobre células mononucleadas de sangre de cordón umbilical |
| dc.title.alternative.spa.fl_str_mv |
Evaluation of two mandioca starches as crioprotectors of monucleus umbilical blood cord cells |
| title |
Evaluación de la actividad crioprotectora de dos almidones de yuca sobre células mononucleadas de sangre de cordón umbilical |
| spellingShingle |
Evaluación de la actividad crioprotectora de dos almidones de yuca sobre células mononucleadas de sangre de cordón umbilical Crioprotección Hidroxietil almidón Almidón de yuca Reológicas Mandioca Almidón Células Criopreservación |
| title_short |
Evaluación de la actividad crioprotectora de dos almidones de yuca sobre células mononucleadas de sangre de cordón umbilical |
| title_full |
Evaluación de la actividad crioprotectora de dos almidones de yuca sobre células mononucleadas de sangre de cordón umbilical |
| title_fullStr |
Evaluación de la actividad crioprotectora de dos almidones de yuca sobre células mononucleadas de sangre de cordón umbilical |
| title_full_unstemmed |
Evaluación de la actividad crioprotectora de dos almidones de yuca sobre células mononucleadas de sangre de cordón umbilical |
| title_sort |
Evaluación de la actividad crioprotectora de dos almidones de yuca sobre células mononucleadas de sangre de cordón umbilical |
| dc.creator.fl_str_mv |
Ortiz Trujillo, Bibiana Ospino, Benjamín Trujillo, Miosotis |
| dc.contributor.author.spa.fl_str_mv |
Ortiz Trujillo, Bibiana Ospino, Benjamín Trujillo, Miosotis |
| dc.subject.proposal.spa.fl_str_mv |
Crioprotección Hidroxietil almidón Almidón de yuca Reológicas |
| topic |
Crioprotección Hidroxietil almidón Almidón de yuca Reológicas Mandioca Almidón Células Criopreservación |
| dc.subject.agrovoc.spa.fl_str_mv |
Mandioca Almidón Células Criopreservación |
| description |
This research determined the possibility to change the hydroxyethyl starch (HES), conventional crioprotector, for two sorts of mandioca starches made by national industry. The study verified the influence of the mandioca starches on the hidrogenions amount in blood and confirmed the estability of starches in umbilical cord blood envelop and established its molecular stability inside a medium with amylases enzymes and mononuclear cells such as the umbilical cordon blood. The mononuclear cells viability six months after being criopreservated with each starch at –80ºC, using HES as a reference or standard, was also assessed. Results showed that the umbilical cord blood, outside the organism for 30 minutes is more affected by the mandioca starches than by HES. In spite of the similar structure of the mandioca starch and HES, the latter one is more resistant in the blood environment to the enzymatic attack of amylases because of its modified structure, therefore it is more resistance to withstand the blood conditions. It was corroborated that HES was able to keep the mononuclear cell viability over 70%, but mandioca starches did not, because of their influence on the pH which became lethal to cells. It was concluded, that the amilopectine of mandioca starches with some modifications in its molecular structure such as hydroxyethylation could work as a crioprotector for mononuclear blood cells, provided that the pH is controlled. |
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2007 |
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2007-07 |
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2019-11-08T16:51:01Z |
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2019-11-08T16:51:01Z |
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Artículo de revista |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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http://purl.org/coar/resource_type/c_6501 |
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info:eu-repo/semantics/article |
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Text |
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0123-4226 |
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https://revistas.udca.edu.co/index.php/ruadc/article/view/591 |
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305282 |
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0123-4226 305282 |
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https://revistas.udca.edu.co/index.php/ruadc/article/view/591 |
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spa |
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spa |
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Revista UDCA : Actualidad & divulgación Científica (Bogotá). -- Vol. 10 No. 2 (Jul.-Dic., 2007). -- páginas 125-133 |
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Agricultura |
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Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales |
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Atribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0) |
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Bogotá : Universidad de Ciencias Aplicadas y Ambientales, 2007 |
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Ortiz Trujillo, BibianaOspino, BenjamínTrujillo, Miosotis2019-11-08T16:51:01Z2019-11-08T16:51:01Z2007-070123-4226https://revistas.udca.edu.co/index.php/ruadc/article/view/591305282This research determined the possibility to change the hydroxyethyl starch (HES), conventional crioprotector, for two sorts of mandioca starches made by national industry. The study verified the influence of the mandioca starches on the hidrogenions amount in blood and confirmed the estability of starches in umbilical cord blood envelop and established its molecular stability inside a medium with amylases enzymes and mononuclear cells such as the umbilical cordon blood. The mononuclear cells viability six months after being criopreservated with each starch at –80ºC, using HES as a reference or standard, was also assessed. Results showed that the umbilical cord blood, outside the organism for 30 minutes is more affected by the mandioca starches than by HES. In spite of the similar structure of the mandioca starch and HES, the latter one is more resistant in the blood environment to the enzymatic attack of amylases because of its modified structure, therefore it is more resistance to withstand the blood conditions. It was corroborated that HES was able to keep the mononuclear cell viability over 70%, but mandioca starches did not, because of their influence on the pH which became lethal to cells. It was concluded, that the amilopectine of mandioca starches with some modifications in its molecular structure such as hydroxyethylation could work as a crioprotector for mononuclear blood cells, provided that the pH is controlled.La presente investigación tuvo con fin evaluar laposibilidad de cambiar el hidroxietil almidón HES,crioprotector convencional, por dos clases de almidonesde yuca producidos por una industria nacional. El estudioverificó la influencia de los almidones en la concentracióndehidrogenionesdelmediosanguíneoysuestabilidaden un ámbito que contiene enzimas amilasasy unalto contenido de células mononucleadas, como loes la sangre de cordón umbilical. Por último, evaluólaviabilidaddelascélulasmononucleadasseismesesdespuésdeunacriopreservaciónconcadaalmidóna–80ºC,teniendocomopatróndecomparaciónelHES.Enlosresultados,seobservóquelasangredecordónumbilical,fueradelorganismopor30minutos,puedemantenerel pH,aunqueelvalorseafectamásconlosalmidonesdeyucaqueconelHES.ApesardequeelalmidónesmuysimilaralHES,encuantoaestructuramolecular,ésteesmásresistentealataqueenzimáticode las amilasas. En la última etapa, se corroboróqueelHESmantieneunaviabilidadcelulardel70%,pero los almidones de yuca, por la influencia sobre la concentración de hidrogeniones, causan muerte celular y evitan la formación de cristales que lisan las células, pero disminuyen al pH a un valor letal para las células. Se concluyó que la amilopectina de los almidones de yuca, con algunas modificaciones en su estructura, como las del tipo hidroxietilación, puede llegar a funcionar como crioprotector para las células mononucleadas en medio sanguíneo, siempre y cuando se controle su baja modificación del pH.Incluye referencias bibliográficasapplication/pdfspaBogotá : Universidad de Ciencias Aplicadas y Ambientales, 2007Revista UDCA : Actualidad & divulgación Científica (Bogotá). -- Vol. 10 No. 2 (Jul.-Dic., 2007). -- páginas 125-133AgriculturaDerechos Reservados - Universidad de Ciencias Aplicadas y Ambientaleshttps://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccessAtribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)http://purl.org/coar/access_right/c_abf2Evaluación de la actividad crioprotectora de dos almidones de yuca sobre células mononucleadas de sangre de cordón umbilicalEvaluation of two mandioca starches as crioprotectors of monucleus umbilical blood cord cellsArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionTexthttp://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85CrioprotecciónHidroxietil almidónAlmidón de yucaReológicasMandiocaAlmidónCélulasCriopreservaciónPublicationORIGINALArtículo.pdfArtículo.pdfapplication/pdf348042https://repository.udca.edu.co/bitstreams/9d13267f-e4c3-4a6f-97a1-7cc2ccd1cea7/downloadd7817c76472e9f3a378c68be8e47d619MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-814775https://repository.udca.edu.co/bitstreams/75b9f8cb-97ad-43d6-b5dc-1475b56fdabc/downloadf661acf14bedbf9f5d13897a0387e751MD52TEXTArtículo.pdf.txtArtículo.pdf.txtExtracted texttext/plain34944https://repository.udca.edu.co/bitstreams/9825ecec-a0ab-47eb-8dab-b27188f52e38/download8f31f9c59a8ed8dd5323c35fa93493adMD53THUMBNAILArtículo.pdf.jpgArtículo.pdf.jpgGenerated 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 nombre de la parte o las partes que el Autor Original y/o el Licenciante hubieren designado para la atribución (v.g., un instituto patrocinador, editorial, publicación) en la información de los derechos de autor del Licenciante, términos de servicios o de otras formas razonables; el título de la Obra si está provisto; en la medida de lo razonablemente factible y, si está provisto, el Identificador Uniforme de Recursos (Uniform Resource Identifier) que el Licenciante especifica para ser asociado con la Obra, salvo que tal URI no se refiera a la nota sobre los derechos de autor o a la información sobre el licenciamiento de la Obra; y en el caso de una Obra Derivada, atribuir el crédito identificando el uso de la Obra en la Obra Derivada (v.g., "Traducción Francesa de la Obra del Autor Original," o "Guión Cinematográfico basado en la Obra original del Autor Original"). Tal crédito puede ser implementado de cualquier forma razonable; en el caso, sin embargo, de Obras Derivadas u Obras Colectivas, tal crédito aparecerá, como mínimo, donde aparece el crédito de cualquier otro autor comparable y de una manera, al menos, tan destacada como el crédito de otro autor comparable.
d.	Para evitar toda confusión, el Licenciante aclara que, cuando la obra es una composición musical:
i.	Regalías por interpretación y ejecución bajo licencias generales. El Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública o la ejecución pública digital de la obra y de recolectar, sea individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, SAYCO), las regalías por la ejecución pública o por la ejecución pública digital de la obra (por ejemplo Webcast) licenciada bajo licencias generales, si la interpretación o ejecución de la obra está primordialmente orientada por o dirigida a la obtención de una ventaja comercial o una compensación monetaria privada.
ii.	Regalías por Fonogramas. El Licenciante se reserva el derecho exclusivo de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, SAYCO), una agencia de derechos musicales o algún agente designado, las regalías por cualquier fonograma que Usted cree a partir de la obra (“versión cover”) y distribuya, en los términos del régimen de derechos de autor, si la creación o distribución de esa versión cover está primordialmente destinada o dirigida a obtener una ventaja comercial o una compensación monetaria privada.
e.	Gestión de Derechos de Autor sobre Interpretaciones y Ejecuciones Digitales (WebCasting). Para evitar toda confusión, el Licenciante aclara que, cuando la obra sea un fonograma, el Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública digital de la obra (por ejemplo, webcast) y de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, Acinpro), las regalías por la ejecución pública digital de la obra (por ejemplo, webcast), sujeta a las disposiciones aplicables del régimen de Derecho de Autor, si esta ejecución pública digital está primordialmente dirigida a obtener una ventaja comercial o una compensación monetaria privada.
5. Representaciones, Garantías y Limitaciones de Responsabilidad.
A MENOS QUE LAS PARTES LO ACORDARAN DE OTRA FORMA POR ESCRITO, EL LICENCIANTE OFRECE LA OBRA (EN EL ESTADO EN EL QUE SE ENCUENTRA) “TAL CUAL”, SIN BRINDAR GARANTÍAS DE CLASE ALGUNA RESPECTO DE LA OBRA, YA SEA EXPRESA, IMPLÍCITA, LEGAL O CUALQUIERA OTRA, INCLUYENDO, SIN LIMITARSE A ELLAS, GARANTÍAS DE TITULARIDAD, COMERCIABILIDAD, ADAPTABILIDAD O ADECUACIÓN A PROPÓSITO DETERMINADO, AUSENCIA DE INFRACCIÓN, DE AUSENCIA DE DEFECTOS LATENTES O DE OTRO TIPO, O LA PRESENCIA O AUSENCIA DE ERRORES, SEAN O NO DESCUBRIBLES (PUEDAN O NO SER ESTOS DESCUBIERTOS). ALGUNAS JURISDICCIONES NO PERMITEN LA EXCLUSIÓN DE GARANTÍAS IMPLÍCITAS, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.
6. Limitación de responsabilidad.
A MENOS QUE LO EXIJA EXPRESAMENTE LA LEY APLICABLE, EL LICENCIANTE NO SERÁ RESPONSABLE ANTE USTED POR DAÑO ALGUNO, SEA POR RESPONSABILIDAD EXTRACONTRACTUAL, PRECONTRACTUAL O CONTRACTUAL, OBJETIVA O SUBJETIVA, SE TRATE DE DAÑOS MORALES O PATRIMONIALES, DIRECTOS O INDIRECTOS, PREVISTOS O IMPREVISTOS PRODUCIDOS POR EL USO DE ESTA LICENCIA O DE LA OBRA, AUN CUANDO EL LICENCIANTE HAYA SIDO ADVERTIDO DE LA POSIBILIDAD DE DICHOS DAÑOS. ALGUNAS LEYES NO PERMITEN LA EXCLUSIÓN DE CIERTA RESPONSABILIDAD, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.
7. Término.
a.	Esta Licencia y los derechos otorgados en virtud de ella terminarán automáticamente si Usted infringe alguna condición establecida en ella. Sin embargo, los individuos o entidades que han recibido Obras Derivadas o Colectivas de Usted de conformidad con esta Licencia, no verán terminadas sus licencias, siempre que estos individuos o entidades sigan cumpliendo íntegramente las condiciones de estas licencias. Las Secciones 1, 2, 5, 6, 7, y 8 subsistirán a cualquier terminación de esta Licencia.
b.	Sujeta a las condiciones y términos anteriores, la licencia otorgada aquí es perpetua (durante el período de vigencia de los derechos de autor de la obra). No obstante lo anterior, el Licenciante se reserva el derecho a publicar y/o estrenar la Obra bajo condiciones de licencia diferentes o a dejar de distribuirla en los términos de esta Licencia en cualquier momento; en el entendido, sin embargo, que esa elección no servirá para revocar esta licencia o que deba ser otorgada , bajo los términos de esta licencia), y esta licencia continuará en pleno vigor y efecto a menos que sea terminada como se expresa atrás. La Licencia revocada continuará siendo plenamente vigente y efectiva si no se le da término en las condiciones indicadas anteriormente.
8. Varios.
a.	Cada vez que Usted distribuya o ponga a disposición pública la Obra o una Obra Colectiva, el Licenciante ofrecerá al destinatario una licencia en los mismos términos y condiciones que la licencia otorgada a Usted bajo esta Licencia.
b.	Si alguna disposición de esta Licencia resulta invalidada o no exigible, según la legislación vigente, esto no afectará ni la validez ni la aplicabilidad del resto de condiciones de esta Licencia y, sin acción adicional por parte de los sujetos de este acuerdo, aquélla se entenderá reformada lo mínimo necesario para hacer que dicha disposición sea válida y exigible.
c.	Ningún término o disposición de esta Licencia se estimará renunciada y ninguna violación de ella será consentida a menos que esa renuncia o consentimiento sea otorgado por escrito y firmado por la parte que renuncie o consienta.
d.	Esta Licencia refleja el acuerdo pleno entre las partes respecto a la Obra aquí licenciada. No hay arreglos, acuerdos o declaraciones respecto a la Obra que no estén especificados en este documento. El Licenciante no se verá limitado por ninguna disposición adicional que pueda surgir en alguna comunicación emanada de Usted. Esta Licencia no puede ser modificada sin el consentimiento mutuo por escrito del Licenciante y Usted.

 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