Protein Supplementation during Mid-Gestation Alters the Amino Acid Patterns, Hepatic Metabolism, and Maternal Skeletal Muscle Turnover of Pregnant Zebu Beef Cows

From 100 to 200 days of gestation, 52 cows carrying male (n = 30) or female (n = 22) fetuses were assigned to CON (basal diet—5.5% of CP, n = 26) or SUP (basal diet + protein supplement [40% CP, 3.5 g/kg BW]—12% of CP, n = 26) treatments. Glucose concentrations decreased at 200 (p ≤ 0.01; CON = 46.9 and SUP = 54.7 mg/dL) and 270 days (p ≤ 0.05; CON = 48.4 and SUP = 53.3 mg/dL) for CON compared to SUP. The same pattern occurred for insulin (p ≤ 0.01). At parturition, the NEFA concentration was greater (p = 0.01, 0.10 vs. 0.08 mmol/L) for CON than for SUP. Total AA increased in SUP (p ≤ 0.03) at mid- and late-gestation compared to CON. At 200 days, CON dams carrying females had less essential AA (p = 0.01) than cows carrying males. The SUP dams had greater expressions of protein synthesis markers, namely eIf4E and GSK3β (p ≤ 0.04), at day 200 and of MuFR1 (protein degradation marker, p ≤ 0.04) at parturition. Supplemented cows had higher hepatic pyruvate carboxylase expressions (p = 0.02). Therefore, PS alleviates the restriction overload on maternal metabolism.

• Autores: Moreno Meneses, Javier Andrés; Batista Nascimento, Karolina; Castilho Galvão, Matheus; Ramírez Zamudio, Germán Darío; Santos Gionbelli, Tathyane Ramalho; Ladeira, Marcio; De Souza Duarte, Marcio; Casagrande, Daniel Rume; Pies Gionbelli, Mateus
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2022
• Institución: Universidad de Ciencias Aplicadas y Ambientales U.D.C.A
• Repositorio: Repositorio Institucional UDCA
• Idioma: eng
• OAI Identifier: oai:repository.udca.edu.co:11158/5011
• Acceso en línea: https://repository.udca.edu.co/handle/11158/5011; https://doi.org/10.3390/ani12243567
• Palabra clave: Sistema Musculoesquelético; Aminoácidos; Glucóneogenesis; Nutrición animal; Cebú
• Rights: closedAccess
• License: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode.es