Cardiac glycosides, phenolic compounds and antioxidant activity from plant cell suspension cultures of Thevetia peruviana
Cell suspension cultures of Thevetia peruviana were established under dark for 19 days to investigate kinetic behavior related to biomass, substrate, cardiac glycoside, polyphenols, reactive oxygen species and anti-oxidant activity. The results showed high biomass production (18.80gDW/L) as well as...
- Autores:
-
Arias, Juan Pablo
Zapata, Karol
Rojano, Benjamín
Peñuela, Mariana
Arias, Mario
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2017
- Institución:
- Universidad de Ciencias Aplicadas y Ambientales U.D.C.A
- Repositorio:
- Repositorio Institucional UDCA
- Idioma:
- eng
- OAI Identifier:
- oai:repository.udca.edu.co:11158/1644
- Acceso en línea:
- https://revistas.udca.edu.co/index.php/ruadc/article/view/393
https://doi.org/10.31910/rudca.v20.n2.2017.393
- Palabra clave:
- Cell cultures
Glycosides
Plant metabolites
Anti - oxidant activity
Reactive oxygen species
Phenols
Plant cell suspension culture
Thevetia peruviana
Plant metabolites
- Rights
- closedAccess
- License
- Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales, 2017
| Summary: | Cell suspension cultures of Thevetia peruviana were established under dark for 19 days to investigate kinetic behavior related to biomass, substrate, cardiac glycoside, polyphenols, reactive oxygen species and anti-oxidant activity. The results showed high biomass production (18.80gDW/L) as well as sucrose consumption in 7 days. Preferential glucose over fructose consumption was observed. Intracellular production of cardiac glycosides reached 2.58mg DE/gDW at day 19. Highest extracellular production was reached between day 2 and 7 (6.19mg DE/L). Highest extracellular phenolic content was 80.61 ± 5.16mg GAE/L at day 7. Intracellular phenolic content increased to 2.76 ± 0.14mg GAE/gFW at day 7 and remained constant until day 19. ROS production at day 7 could be related to sucrose and glucose total consumption. Pearson Product-Moment Correlation Coefficient (ρ) showed that the phenolic compounds in cell suspension cultures of T. peruviana were responsible for the observed anti-oxidant activity. All together, these results give the first steps in metabolic behavior in cell suspension cultures of T. peruviana.Key words: Plant metabolites, Anti-oxidant activity, Reactive Oxygen Species, Phenols, Plant cell suspension culture, Thevetia peruviana. |
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