Structural and functional characterization of toxic peptides purified from the venom of the Colombian scorpion Tityus macrochirus

The soluble venom of the scorpion Tityus macrochirus was separated by chromatographic procedures and three homogeneous peptides were obtained and their primary structures were determined. They were called: Tma1-Tma3, from the abbreviated name of the scorpion. Tma1 is a peptide containing 65 amino ac...

Full description

Autores:
Rincón Cortés, Clara Andrea
Olamendi Portugal, Timoteo
Carcamo Noriega, Edson Norberto
González Santillán, Edmundo
Zamudio Zuñiga, Frnando
Reyes Montaño, Edgar Antonio
Vega Castro, Nohora Angélica
Possani, Lourival Domingos
Tipo de recurso:
Article of journal
Fecha de publicación:
2019
Institución:
Universidad de Ciencias Aplicadas y Ambientales U.D.C.A
Repositorio:
Repositorio Institucional UDCA
Idioma:
eng
OAI Identifier:
oai:repository.udca.edu.co:11158/2077
Acceso en línea:
https://www.scopus.com/search/form.uri?display=basic
Palabra clave:
Secuencia de Aminoácidos
Canales de Sodio
Péptidos
Venenos de Escorpión
Amino acid sequence
Phylogenetic tree
Scorpion toxin
Sodium-channels
Tityus macrochirus
Rights
openAccess
License
Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales
Description
Summary:The soluble venom of the scorpion Tityus macrochirus was separated by chromatographic procedures and three homogeneous peptides were obtained and their primary structures were determined. They were called: Tma1-Tma3, from the abbreviated name of the scorpion. Tma1 is a peptide containing 65 amino acids with four disulfide linkages and a molecular weight of 7386.2 Da. It is a mammalian toxin, shown to affect human sodium-channels sub-types hNav1.6 and hNav1.4. Tma2 and Tma3 are peptides containing 69 amino acids linked by four disulfide bonds, molecular weights 7819.7 and 7830.0 Da, respectively. They do not affect human sodium-channels but are lethal to insects (crickets). A phylogenic analysis of the three peptides and those of other toxic peptides isolated from the genus Tityus and Centruroides were grouped together and analyzed, permitting to obtain a topology with two main clades, one includes most sodium-channel anti-insect scorpion toxins and others includes mostly sodium-channel scorpion toxins anti-mammalian. Tma1 segregates among a group of well-studied β-class toxins of other Tityus species such as T. discrepans, T. obscurus and T. pachyurus. Tma2 and Tma3 are associated with anti-insect toxins, particularly with one of T. obscurus. This phylogenetic analysis confirms and enforces our experimental results obtained with these three new sodium-channel scorpion toxins.