Identifying major histocompatibility complex class II-DR molecules in bovine and swine peripheral blood monocyte-derived macrophages using mAb-L243

Major histocompatibility complex class II (MHC-II) molecules are involved in immune responses against pathogens and vaccine candidates’ immunogenicity. Immunopeptidomics for identifying cancer and infection-related antigens and epitopes have benefited from advances in immunopurification methods and mass spectrometry analysis. The mouse anti-MHC-II-DR monoclonal antibody L243 (mAb-L243) has been effective in recognising MHC-II-DR in both human and non-human primates. It has also been shown to cross-react with other animal species, although it has not been tested in livestock. This study used mAb-L243 to identify Staphylococcus aureus and Salmonella enterica serovar Typhimurium peptides binding to cattle and swine macrophage MHC-II-DR molecules using flow cytometry, mass spectrometry and two immunopurification techniques. Antibody crossreactivity led to identifying expressed MHC-II-DR molecules, together with 10 Staphylococcus aureus peptides in cattle and 13 S. enterica serovar Typhimurium peptides in swine. Such data demonstrates that MHC-II-DR expression and immunocapture approaches using L243 mAb represents a viable strategy for flow cytometry and immunopeptidomics analysis of bovine and swine antigen-presenting cells.

• Autores: Celis Giraldo, Carmen Teresa; Ordoñez, Diego; Diaz-Arevalo, Diana; Bohórquez Abreo, Michel David; Ibarrola, Nieves; Suárez Martínez, Carlos Fernando; Rodríguez, Kewin; Yepes-Pérez, Yoelis; Rodriguez, Alexander; Avendaño, Catalina; López-Abán, Julio; Manzano ´Román, Raúl; Patarroyo, Manuel-Alfonso
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2024
• Institución: Universidad de Ciencias Aplicadas y Ambientales U.D.C.A
• Repositorio: Repositorio Institucional UDCA
• Idioma: eng
• OAI Identifier: oai:repository.udca.edu.co:11158/5767
• Acceso en línea: https://repository.udca.edu.co/handle/11158/5767; https://doi.org/10.1016/j.vaccine.2024.04.042
• Palabra clave: Péptidos; Cerdos; Complejo Mayor de Histocompatibilidad; Immunopeptidomics; Bovinos; Macrofagos
• Rights: openAccess
• License: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode.es