Satellite DNA methylation status and expression of selected genes in Bos indicus blastocysts produced in vivo and in vitro
Bovine embryos produced in vivo and in vitro differ with respect to molecular profiles, including epigenetic marks and gene expression profiles. This study investigated the CpG methylation status in bovine testis satellite I (BTS) and Bos taurus alpha satellite I (BTαS) DNA sequences, and concomitan...
- Autores:
-
Urrego, R.
Bernal Uloa, S. M.
Chavarría, N. A.
Herrera-Puerta, E.
Lucas-Hahn, A.
Herrmann, D.
Winkler, S.
Pache, D.
Niemann, H.
Rodriguez-Osorio, N.
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2017
- Institución:
- Universidad de Ciencias Aplicadas y Ambientales U.D.C.A
- Repositorio:
- Repositorio Institucional UDCA
- Idioma:
- eng
- OAI Identifier:
- oai:repository.udca.edu.co:11158/2351
- Acceso en línea:
- https://www.scopus.com/search/form.uri?display=basic
- Palabra clave:
- Células
Bovinos
Blastocito
ADN (Citosina-5-) Metiltransferasa
Metilación de ADN
ADN Satélite
Bovinae
Embryonic Structures
Cells
Blastocyst
DNA (Cytosine-5-)-Methyltransferase
DNA Methylation
DNA, Satellite
Estructuras embrionarias
- Rights
- openAccess
- License
- Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales
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| dc.title.spa.fl_str_mv |
Satellite DNA methylation status and expression of selected genes in Bos indicus blastocysts produced in vivo and in vitro |
| title |
Satellite DNA methylation status and expression of selected genes in Bos indicus blastocysts produced in vivo and in vitro |
| spellingShingle |
Satellite DNA methylation status and expression of selected genes in Bos indicus blastocysts produced in vivo and in vitro Células Bovinos Blastocito ADN (Citosina-5-) Metiltransferasa Metilación de ADN ADN Satélite Bovinae Embryonic Structures Cells Blastocyst DNA (Cytosine-5-)-Methyltransferase DNA Methylation DNA, Satellite Estructuras embrionarias |
| title_short |
Satellite DNA methylation status and expression of selected genes in Bos indicus blastocysts produced in vivo and in vitro |
| title_full |
Satellite DNA methylation status and expression of selected genes in Bos indicus blastocysts produced in vivo and in vitro |
| title_fullStr |
Satellite DNA methylation status and expression of selected genes in Bos indicus blastocysts produced in vivo and in vitro |
| title_full_unstemmed |
Satellite DNA methylation status and expression of selected genes in Bos indicus blastocysts produced in vivo and in vitro |
| title_sort |
Satellite DNA methylation status and expression of selected genes in Bos indicus blastocysts produced in vivo and in vitro |
| dc.creator.fl_str_mv |
Urrego, R. Bernal Uloa, S. M. Chavarría, N. A. Herrera-Puerta, E. Lucas-Hahn, A. Herrmann, D. Winkler, S. Pache, D. Niemann, H. Rodriguez-Osorio, N. |
| dc.contributor.author.spa.fl_str_mv |
Urrego, R. Bernal Uloa, S. M. Chavarría, N. A. Herrera-Puerta, E. Lucas-Hahn, A. Herrmann, D. Winkler, S. Pache, D. Niemann, H. Rodriguez-Osorio, N. |
| dc.subject.mesh.spa.fl_str_mv |
Células Bovinos Blastocito ADN (Citosina-5-) Metiltransferasa Metilación de ADN ADN Satélite |
| topic |
Células Bovinos Blastocito ADN (Citosina-5-) Metiltransferasa Metilación de ADN ADN Satélite Bovinae Embryonic Structures Cells Blastocyst DNA (Cytosine-5-)-Methyltransferase DNA Methylation DNA, Satellite Estructuras embrionarias |
| dc.subject.proposal.spa.fl_str_mv |
Bovinae Embryonic Structures Cells Blastocyst DNA (Cytosine-5-)-Methyltransferase DNA Methylation DNA, Satellite |
| dc.subject.agrovoc.spa.fl_str_mv |
Estructuras embrionarias |
| description |
Bovine embryos produced in vivo and in vitro differ with respect to molecular profiles, including epigenetic marks and gene expression profiles. This study investigated the CpG methylation status in bovine testis satellite I (BTS) and Bos taurus alpha satellite I (BTαS) DNA sequences, and concomitantly the relative abundance of transcripts, critically involved in DNA methylation (DNMT1 and DNMT3A), growth and development (IGF2R) and pluripotency (POU5F1) in Bos indicus embryos produced in vitro or in vivo. Results revealed that methylation of BTS were higher (P < 0.05) in embryos produced in vitro compared with their in vivo produced counterparts, while the methylation status of BTαS was similar in both groups. There were no significant differences in transcript abundance for DNMT3A, IGF2R and POU5F1 between blastocysts produced in vivo and in vitro. However, a significantly lower amount of DNMT1 transcripts was found in the in vitro cultured embryos (P < 0.05) compared with their in vivo derived counterparts. In conclusion, this study reported only minor changes in the expression of developmentally important genes and satellite DNA methylation related to the in vitro embryo production system. |
| publishDate |
2017 |
| dc.date.issued.spa.fl_str_mv |
2017 |
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2019-10-24T22:02:37Z |
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2019-10-24T22:02:37Z |
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Artículo de revista |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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http://purl.org/coar/resource_type/c_6501 |
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Urrego, R., Bernal-Ulloa, S., Chavarría, ,N.A., Herrera-Puerta, E., Lucas-Hahn, A., Herrmann, D., . . . Rodriguez-Osorio, N. (2017). Satellite DNA methylation status and expression of selected genes in bos indicus blastocysts produced in vivo and in vitro. Zygote, 25(2), 131-140. doi:http://dx.doi.org/10.1017/S096719941600040X |
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https://www.scopus.com/search/form.uri?display=basic |
| dc.identifier.doi.spa.fl_str_mv |
10.1017/S096719941600040X |
| identifier_str_mv |
Urrego, R., Bernal-Ulloa, S., Chavarría, ,N.A., Herrera-Puerta, E., Lucas-Hahn, A., Herrmann, D., . . . Rodriguez-Osorio, N. (2017). Satellite DNA methylation status and expression of selected genes in bos indicus blastocysts produced in vivo and in vitro. Zygote, 25(2), 131-140. doi:http://dx.doi.org/10.1017/S096719941600040X 10.1017/S096719941600040X |
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https://www.scopus.com/search/form.uri?display=basic |
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eng |
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eng |
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Zygote;Vol. 25, No. 2 Abr. 1 2017 páginas 131-140 |
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Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales |
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http://purl.org/coar/access_right/c_abf2 |
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Atribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0) |
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Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales https://creativecommons.org/licenses/by-nc-sa/4.0/ Atribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0) http://purl.org/coar/access_right/c_abf2 |
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Urrego, R.Bernal Uloa, S. M.Chavarría, N. A.Herrera-Puerta, E.Lucas-Hahn, A.Herrmann, D.Winkler, S.Pache, D.Niemann, H.Rodriguez-Osorio, N.2019-10-24T22:02:37Z2019-10-24T22:02:37Z2017Urrego, R., Bernal-Ulloa, S., Chavarría, ,N.A., Herrera-Puerta, E., Lucas-Hahn, A., Herrmann, D., . . . Rodriguez-Osorio, N. (2017). Satellite DNA methylation status and expression of selected genes in bos indicus blastocysts produced in vivo and in vitro. Zygote, 25(2), 131-140. doi:http://dx.doi.org/10.1017/S096719941600040Xhttps://www.scopus.com/search/form.uri?display=basic10.1017/S096719941600040XBovine embryos produced in vivo and in vitro differ with respect to molecular profiles, including epigenetic marks and gene expression profiles. This study investigated the CpG methylation status in bovine testis satellite I (BTS) and Bos taurus alpha satellite I (BTαS) DNA sequences, and concomitantly the relative abundance of transcripts, critically involved in DNA methylation (DNMT1 and DNMT3A), growth and development (IGF2R) and pluripotency (POU5F1) in Bos indicus embryos produced in vitro or in vivo. Results revealed that methylation of BTS were higher (P < 0.05) in embryos produced in vitro compared with their in vivo produced counterparts, while the methylation status of BTαS was similar in both groups. There were no significant differences in transcript abundance for DNMT3A, IGF2R and POU5F1 between blastocysts produced in vivo and in vitro. However, a significantly lower amount of DNMT1 transcripts was found in the in vitro cultured embryos (P < 0.05) compared with their in vivo derived counterparts. In conclusion, this study reported only minor changes in the expression of developmentally important genes and satellite DNA methylation related to the in vitro embryo production system.application/pdfengZygote;Vol. 25, No. 2 Abr. 1 2017 páginas 131-140Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientaleshttps://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccessAtribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)http://purl.org/coar/access_right/c_abf2https://udca.elogim.com:2092/record/display.uri?eid=2-s2.0-85011311208&origin=resultslist&sort=plf-f&src=s&nlo=1&nlr=20&nls=afprfnm-t&affilName=Universidad+de+Ciencias+Aplicadas+y+Ambientales&sid=ebaab1bcd4dbde71ac2479a686752d77&sot=afnl&sdt=cl&cluster=scopubyr%2c%222017%22%2ct&sl=67&s=%28AF-ID%28%22Universidad+de+Ciencias+Aplicadas+y+Ambientales%22+60106980%29%29&relpos=34&citeCnt=4&searchTerm=CélulasBovinosBlastocitoADN (Citosina-5-) MetiltransferasaMetilación de ADNADN SatéliteBovinaeEmbryonic StructuresCellsBlastocystDNA (Cytosine-5-)-MethyltransferaseDNA MethylationDNA, SatelliteEstructuras embrionariasSatellite DNA methylation status and expression of selected genes in Bos indicus blastocysts produced in vivo and in vitroArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionTexthttp://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85PublicationLICENSElicense.txtlicense.txttext/plain; charset=utf-814775https://repository.udca.edu.co/bitstreams/926f8ee0-eece-471e-be31-123c8eb66e73/downloadf661acf14bedbf9f5d13897a0387e751MD52ORIGINALout.pdfout.pdfapplication/pdf272317https://repository.udca.edu.co/bitstreams/c0d004da-f036-4e62-b6ec-3f353a832774/download8cbdece80ca0290108dfe09b590bf821MD51TEXTout.pdf.txtout.pdf.txtExtracted texttext/plain46901https://repository.udca.edu.co/bitstreams/21c2b65d-442a-4b8d-8249-be0cec16e1e1/downloadbe4f471acb3bd80278c80b4272a84a18MD53THUMBNAILout.pdf.jpgout.pdf.jpgGenerated Thumbnailimage/jpeg7009https://repository.udca.edu.co/bitstreams/7f5ee954-2dbe-4444-a856-3e668850b63f/downloadf5b279466345e13785b340b2726f2773MD5411158/2351oai:repository.udca.edu.co:11158/23512024-05-09 14:21:56.324https://creativecommons.org/licenses/by-nc-sa/4.0/Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientalesopen.accesshttps://repository.udca.edu.coRepositorio - Universidad de Ciencias Aplicadas y Ambientales UDCA.bdigital@metabiblioteca.com |