Síntesis de copolímeros anfifílicos bioconjugados con colesterol y su aplicación en la encapsulación y liberación de anfotericina B
En el presente trabajo se estudió la síntesis de copolímeros en bloque anfifílicos basados en acrilato de colesterilo, acrilato de butilo y polietilenglicol mediante la técnica ARGET-ATRP, la polimerización fue confirmada empleando FT-IR, RMN1H, GPC y DSC. Adicionalmente, se estableció el efecto de...
- Autores:
-
Rodríguez Molina, Yeimy Johana
- Tipo de recurso:
- Trabajo de grado de pregrado
- Fecha de publicación:
- 2016
- Institución:
- Universidad de Ciencias Aplicadas y Ambientales U.D.C.A
- Repositorio:
- Repositorio Institucional UDCA
- Idioma:
- spa
- OAI Identifier:
- oai:repository.udca.edu.co:11158/565
- Acceso en línea:
- https://repository.udca.edu.co/handle/11158/565
- Palabra clave:
- Copolímeros Anfífilicos
Colesterol
Anfotericina B
Copolimeros de bloque
Anfotericina B
Química
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- closedAccess
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- Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales
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Síntesis de copolímeros anfifílicos bioconjugados con colesterol y su aplicación en la encapsulación y liberación de anfotericina B |
| title |
Síntesis de copolímeros anfifílicos bioconjugados con colesterol y su aplicación en la encapsulación y liberación de anfotericina B |
| spellingShingle |
Síntesis de copolímeros anfifílicos bioconjugados con colesterol y su aplicación en la encapsulación y liberación de anfotericina B Copolímeros Anfífilicos Colesterol Anfotericina B Copolimeros de bloque Anfotericina B Química |
| title_short |
Síntesis de copolímeros anfifílicos bioconjugados con colesterol y su aplicación en la encapsulación y liberación de anfotericina B |
| title_full |
Síntesis de copolímeros anfifílicos bioconjugados con colesterol y su aplicación en la encapsulación y liberación de anfotericina B |
| title_fullStr |
Síntesis de copolímeros anfifílicos bioconjugados con colesterol y su aplicación en la encapsulación y liberación de anfotericina B |
| title_full_unstemmed |
Síntesis de copolímeros anfifílicos bioconjugados con colesterol y su aplicación en la encapsulación y liberación de anfotericina B |
| title_sort |
Síntesis de copolímeros anfifílicos bioconjugados con colesterol y su aplicación en la encapsulación y liberación de anfotericina B |
| dc.creator.fl_str_mv |
Rodríguez Molina, Yeimy Johana |
| dc.contributor.advisor.spa.fl_str_mv |
Pérez Pérez, León Darío, dir. |
| dc.contributor.author.spa.fl_str_mv |
Rodríguez Molina, Yeimy Johana |
| dc.contributor.corporatename.spa.fl_str_mv |
Universidad de Ciencias Aplicadas y Ambientales, UDCA |
| dc.subject.spa.fl_str_mv |
Copolímeros Anfífilicos Colesterol Anfotericina B |
| topic |
Copolímeros Anfífilicos Colesterol Anfotericina B Copolimeros de bloque Anfotericina B Química |
| dc.subject.lemb.spa.fl_str_mv |
Copolimeros de bloque Anfotericina B Química |
| description |
En el presente trabajo se estudió la síntesis de copolímeros en bloque anfifílicos basados en acrilato de colesterilo, acrilato de butilo y polietilenglicol mediante la técnica ARGET-ATRP, la polimerización fue confirmada empleando FT-IR, RMN1H, GPC y DSC. Adicionalmente, se estableció el efecto de la estructura de los copolímeros en el autoensamblaje en medio acuoso para la formación de micelas y sus propiedades coloidales mediante análisis de DLS, TEM y potencial Z. Finalmente, se estudió la capacidad de las micelas poliméricas bioconjugadas con colesterol, para encapsular y liberar anfotericina B (anfB) empleando espectroscopia UV/VIS. De acuerdo a los resultados obtenidos, propiedades como el diámetro hidrodinámico y morfología de las micelas no se ven afectados por la composición de los copolímeros. Así mismo, al estudiar la encapsulación y liberación de anfB, se encontró que estas propiedades dependen en gran medida del carácter hidrofóbico del copolímero. Alcanzando mayor encapsulación del fármaco a medida que aumenta el contenido de colesterol, mientras que en los estudios de liberación se evidencio que a menor contenido de colesterol la liberación del fármaco es mayor. |
| publishDate |
2016 |
| dc.date.accessioned.spa.fl_str_mv |
2016-09-09T15:52:40Z |
| dc.date.available.spa.fl_str_mv |
2016-09-09T15:52:40Z |
| dc.date.issued.spa.fl_str_mv |
2016 |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Pregrado |
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Strong solubilization of small molecules by triblockcopolymer micelles in selective solvents. Macromolecules, 30(6), 1711–1717. Yan, Q., Yuan, J., Zhang, F., Sui, X., Xie, X., Yin, Y., … Wei, Y. (2009). Cellulosebased dual graft molecular brushes as potential drug nanocarriers: Stimulusresponsive micelles, self-assembled phase transition behavior, and tunable crystalline morphologies. Biomacromolecules, 10(8), 2033–2042. Zhang, X., Zhu, X., Ke, F., Ye, L., Chen, E. qiang, Zhang, A. ying, & Feng, Z. guo. (2009). Preparation and self-assembly of amphiphilic triblock copolymers with polyrotaxane as a middle block and their application as carrier for the controlled release of Amphotericin B. Polymer, 50(18), 4343–4351. |
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Pérez Pérez, León Darío, dir.Rodríguez Molina, Yeimy JohanaUniversidad de Ciencias Aplicadas y Ambientales, UDCA2016-09-09T15:52:40Z2016-09-09T15:52:40Z2016https://repository.udca.edu.co/handle/11158/565En el presente trabajo se estudió la síntesis de copolímeros en bloque anfifílicos basados en acrilato de colesterilo, acrilato de butilo y polietilenglicol mediante la técnica ARGET-ATRP, la polimerización fue confirmada empleando FT-IR, RMN1H, GPC y DSC. Adicionalmente, se estableció el efecto de la estructura de los copolímeros en el autoensamblaje en medio acuoso para la formación de micelas y sus propiedades coloidales mediante análisis de DLS, TEM y potencial Z. Finalmente, se estudió la capacidad de las micelas poliméricas bioconjugadas con colesterol, para encapsular y liberar anfotericina B (anfB) empleando espectroscopia UV/VIS. De acuerdo a los resultados obtenidos, propiedades como el diámetro hidrodinámico y morfología de las micelas no se ven afectados por la composición de los copolímeros. Así mismo, al estudiar la encapsulación y liberación de anfB, se encontró que estas propiedades dependen en gran medida del carácter hidrofóbico del copolímero. Alcanzando mayor encapsulación del fármaco a medida que aumenta el contenido de colesterol, mientras que en los estudios de liberación se evidencio que a menor contenido de colesterol la liberación del fármaco es mayor.PregradoQuímico(a)pdfspaDerechos Reservados - Universidad de Ciencias Aplicadas y Ambientaleshttps://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/closedAccessAtribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)http://purl.org/coar/access_right/c_14cbCopolímeros AnfífilicosColesterolAnfotericina BCopolimeros de bloqueAnfotericina BQuímicaSíntesis de copolímeros anfifílicos bioconjugados con colesterol y su aplicación en la encapsulación y liberación de anfotericina BTrabajo de grado - Pregradohttp://purl.org/coar/resource_type/c_7a1finfo:eu-repo/semantics/bachelorThesisinfo:eu-repo/semantics/publishedVersionhttp://purl.org/redcol/resource_type/TPhttp://purl.org/coar/version/c_970fb48d4fbd8a85Adams, M., & Kwon, G. S. (2004). 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Polymer, 50(18), 4343–4351.Facultad de CienciasQuímicaPublicationTEXTTRABAJO DE GRADO FINAL_Yeimy Rodriguez.pdf.txtTRABAJO DE GRADO FINAL_Yeimy Rodriguez.pdf.txtExtracted texttext/plain97320https://repository.udca.edu.co/bitstreams/c399f36d-ddc1-4b99-b71e-cfc7934bba8b/downloade2c69c7656b97c1aa7df31af26718c01MD53THUMBNAILTRABAJO DE GRADO FINAL_Yeimy Rodriguez.pdf.jpgTRABAJO DE GRADO FINAL_Yeimy Rodriguez.pdf.jpgGenerated Thumbnailimage/jpeg3800https://repository.udca.edu.co/bitstreams/d6351d38-e978-4b52-97ea-b56f27e6998f/download2f74f9efb1f35a4eba0119b6d20770efMD54ORIGINALTRABAJO DE GRADO FINAL_Yeimy Rodriguez.pdfTRABAJO DE GRADO FINAL_Yeimy Rodriguez.pdfapplication/pdf2802964https://repository.udca.edu.co/bitstreams/335749bf-b4d1-401b-9b60-f1794a7d6163/download132b7d38a849ee0df2f55d169b9bdd3eMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repository.udca.edu.co/bitstreams/9202d661-2fc1-4b9f-ac11-a0cd035f98b3/download8a4605be74aa9ea9d79846c1fba20a33MD5211158/565oai:repository.udca.edu.co:11158/5652024-05-09 14:31:22.123https://creativecommons.org/licenses/by-nc-sa/4.0/Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientalesopen.accesshttps://repository.udca.edu.coRepositorio - Universidad de Ciencias Aplicadas y Ambientales UDCA.bdigital@metabiblioteca.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 |