Effects of BPA on expression of apoptotic genes and migration of ovine trophectoderm (oTr1) cells during the peri-implantation period of pregnancy

Bisphenol A (BPA) is an endocrine-disrupting chemical used in the manufacture of many products used daily. In the present study, the effects of BPA (1 × 10 −4 to 1 × 10 -9 M) on migration and on the expression of some apoptotic genes were examined in vitro using ovine trophectoderm (oTr1) primary cell line. The results revealed that BPA at 1 × 10 −9 , 1 × 10 −8 and 1 × 10 −7 M increased migration of oTr1 cells, while 1 × 10 −6 , 1 × 10 −5 and 1 × 10 −4 M BPA decreased cell migration. Regarding apoptosis, expression of the anti-apoptotic gene Bcl-2 mRNA was greater at 1 × 10 −8 and 1 × 10 −9 M BPA and was down-regulated at 1 × 10 −4 to 1 × 10 −7 M BPA; however, expression of pro-apoptotic genes (Bax, cathepsin B, caspase-3 and c-myc) was reduced at the higher concentrations of BPA. Results of this study suggest that BPA may impair implantation by decreasing migration of oTr1 cells and inhibiting apoptosis.

• Autores: Elmetwally, Mohammed A.; Halawa, Amal A.; Lenis, Yaser Y.; Tang, Wanjin; Wu, Guoyao; Bazer, Fuller W.
• Tipo de recurso: Article of journal
• Fecha de publicación: 2019
• Institución: Universidad de Ciencias Aplicadas y Ambientales U.D.C.A
• Repositorio: Repositorio Institucional UDCA
• Idioma: eng
• OAI Identifier: oai:repository.udca.edu.co:11158/2058
• Acceso en línea: https://www.scopus.com/search/form.uri?display=basic
• Palabra clave: Apoptosis; Toxicidad; Fenoles; Disruptores Endocrinos; Movimiento Celular; Apoptosis; BPA; Migration; Pregnancy; Toxicity; Ovinos
• Rights: openAccess
• License: Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales