Evaluación de la biodegradación del colorante azul brillante utilizando hongos de la podredumbre blanca y sus consorcios
Colored effluent from the textile industry cause adverse effects on aquatic ecosystems, leading to a deterioration of their aesthetic and a decrease in photosynthetic processes. In this study, the biodegradation of textile dye brilliant blue, BB, over flowers waste using three fungal strains Pleurot...
- Autores:
-
Rojas, Julián
Hormaza, Angelina
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2016
- Institución:
- Universidad de Ciencias Aplicadas y Ambientales U.D.C.A
- Repositorio:
- Repositorio Institucional UDCA
- Idioma:
- spa
- OAI Identifier:
- oai:repository.udca.edu.co:11158/1757
- Acceso en línea:
- https://revistas.udca.edu.co/index.php/ruadc/article/view/258
https://doi.org/10.31910/rudca.v19.n1.2016.258
- Palabra clave:
- Biorremediación
Colorantes trifenilmetánicos
Microorganismos fúngicos
Actividad enzimática
Biodegradabilidad
Trametes
Pleurotus ostreatus
- Rights
- openAccess
- License
- Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales, 2016
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Evaluación de la biodegradación del colorante azul brillante utilizando hongos de la podredumbre blanca y sus consorcios |
| dc.title.alternative.spa.fl_str_mv |
Evaluation of brilliant blue dye biodegradation by white rot fungi and their consortia |
| title |
Evaluación de la biodegradación del colorante azul brillante utilizando hongos de la podredumbre blanca y sus consorcios |
| spellingShingle |
Evaluación de la biodegradación del colorante azul brillante utilizando hongos de la podredumbre blanca y sus consorcios Biorremediación Colorantes trifenilmetánicos Microorganismos fúngicos Actividad enzimática Biodegradabilidad Trametes Pleurotus ostreatus |
| title_short |
Evaluación de la biodegradación del colorante azul brillante utilizando hongos de la podredumbre blanca y sus consorcios |
| title_full |
Evaluación de la biodegradación del colorante azul brillante utilizando hongos de la podredumbre blanca y sus consorcios |
| title_fullStr |
Evaluación de la biodegradación del colorante azul brillante utilizando hongos de la podredumbre blanca y sus consorcios |
| title_full_unstemmed |
Evaluación de la biodegradación del colorante azul brillante utilizando hongos de la podredumbre blanca y sus consorcios |
| title_sort |
Evaluación de la biodegradación del colorante azul brillante utilizando hongos de la podredumbre blanca y sus consorcios |
| dc.creator.fl_str_mv |
Rojas, Julián Hormaza, Angelina |
| dc.contributor.author.spa.fl_str_mv |
Rojas, Julián Hormaza, Angelina |
| dc.subject.proposal.spa.fl_str_mv |
Biorremediación Colorantes trifenilmetánicos Microorganismos fúngicos Actividad enzimática |
| topic |
Biorremediación Colorantes trifenilmetánicos Microorganismos fúngicos Actividad enzimática Biodegradabilidad Trametes Pleurotus ostreatus |
| dc.subject.agrovoc.spa.fl_str_mv |
Biodegradabilidad Trametes Pleurotus ostreatus |
| description |
Colored effluent from the textile industry cause adverse effects on aquatic ecosystems, leading to a deterioration of their aesthetic and a decrease in photosynthetic processes. In this study, the biodegradation of textile dye brilliant blue, BB, over flowers waste using three fungal strains Pleurotus ostreatus (Po), Pleurotus pulmonarius (Pp), Trametes versicolor (Tv) and some of their consortia was evaluated. The best conditions of degradation were determined by solid state fermentation with individual fungal species and two of its consortia C1: Po -T v y C2: Po-Pp, through a factorial design. Likewise, the kinetics of the process was evaluated and the enzyme activity was quantified. A biodegradation percentage from BB of 99.14% was reached by Pp as single strain, under a ratio of C:N of 20:1, with four biomass plugs and of 99.19% for C2, under a ratio of C:N of 40:1, with four biomass plugs in 1:1 ratio of each selected fungus. As to the kinetics, it was found that C2 required a time of 10 days to reach 92% of BB decolourisation; this same percentage was obtained by Pp at 12 days. Regarding to the involved enzymes, it was found that the highest activity was recorded for the laccase by both individual strains and fungal consortia, with values of 6.98 ULAC/gss and 17.83 ULAC/gss respectively. |
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2016 |
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2016-01 |
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2019-07-09T15:17:15Z |
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2019-07-09T15:17:15Z |
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https://doi.org/10.31910/rudca.v19.n1.2016.258 |
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304504 |
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https://revistas.udca.edu.co/index.php/ruadc/article/view/258 https://doi.org/10.31910/rudca.v19.n1.2016.258 |
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Revista UDCA : Actualidad & Divulgación Científica (Bogotá). -- Vol. 19, No. 1 (Ene.-Jun. 2016). -- páginas 139-148 |
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Agricultura |
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Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales, 2016 |
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Rojas, JuliánHormaza, Angelina2019-07-09T15:17:15Z2019-07-09T15:17:15Z2016-010123-4226https://revistas.udca.edu.co/index.php/ruadc/article/view/258https://doi.org/10.31910/rudca.v19.n1.2016.258304504Colored effluent from the textile industry cause adverse effects on aquatic ecosystems, leading to a deterioration of their aesthetic and a decrease in photosynthetic processes. In this study, the biodegradation of textile dye brilliant blue, BB, over flowers waste using three fungal strains Pleurotus ostreatus (Po), Pleurotus pulmonarius (Pp), Trametes versicolor (Tv) and some of their consortia was evaluated. The best conditions of degradation were determined by solid state fermentation with individual fungal species and two of its consortia C1: Po -T v y C2: Po-Pp, through a factorial design. Likewise, the kinetics of the process was evaluated and the enzyme activity was quantified. A biodegradation percentage from BB of 99.14% was reached by Pp as single strain, under a ratio of C:N of 20:1, with four biomass plugs and of 99.19% for C2, under a ratio of C:N of 40:1, with four biomass plugs in 1:1 ratio of each selected fungus. As to the kinetics, it was found that C2 required a time of 10 days to reach 92% of BB decolourisation; this same percentage was obtained by Pp at 12 days. Regarding to the involved enzymes, it was found that the highest activity was recorded for the laccase by both individual strains and fungal consortia, with values of 6.98 ULAC/gss and 17.83 ULAC/gss respectively.Los efluentes coloreados procedentes de la industria textil causan efectos adversos en los ecosistemas acuáticos, que conducen a un deterioro de su estética y a una disminución de los procesos fotosintéticos. En este trabajo, se evaluó la biodegradación del colorante textil azul brillante AB, sobre residuos de flores, empleando tres cepas fúngicas: Pleurotus ostreatus (Po), Pleurotus pulmonarius (Pp) y Trametes versicolor (Tv), así como algunos de sus consorcios. Las mejores condiciones, se determinaron mediante fermentación en estado sólido, con las especies fúngicas individuales y con dos de sus consorcios fúngicos: C1: Po-Tv y C2: Po-Pp, a través de un diseño factorial. Asimismo, se evaluó la cinética del proceso y se cuantificó la actividad enzimática. Se alcanzó un porcentaje de biodegradación del AB del 99,14%, con Pp, como cepa individual, bajo una relación de carbono:nitrógeno (C:N) de 20:1, con cuatro discos de biomasa y del 99,19%, para el C2, bajo una relación de C:N de 40:1, con cuatro discos de biomasa, en relación 1:1 de cada hongo seleccionado. En cuanto a la cinética, se encontró que el C2 requirió 10 días para alcanzar una degradación del 92% del AB; este mismo porcentaje fue obtenido con Pp a los 12 días. Con respecto a las enzimas involucradas, se encontró que la mayor actividad fue registrada para la lacasa, tanto en cepas individuales como en los consorcios fúngicos, con valores de 6,98 ULac/gss y 17,83 ULac/gss, respectivamente.Incluye referencias bibliográficasapplication/pdfspaBogotá : Universidad de Ciencias Aplicadas y Ambientales, 2016Revista UDCA : Actualidad & Divulgación Científica (Bogotá). -- Vol. 19, No. 1 (Ene.-Jun. 2016). -- páginas 139-148AgriculturaDerechos Reservados - Universidad de Ciencias Aplicadas y Ambientales, 2016https://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccessAtribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)http://purl.org/coar/access_right/c_abf2Evaluación de la biodegradación del colorante azul brillante utilizando hongos de la podredumbre blanca y sus consorciosEvaluation of brilliant blue dye biodegradation by white rot fungi and their consortiaArtículo de 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 nombre de la parte o las partes que el Autor Original y/o el Licenciante hubieren designado para la atribución (v.g., un instituto patrocinador, editorial, publicación) en la información de los derechos de autor del Licenciante, términos de servicios o de otras formas razonables; el título de la Obra si está provisto; en la medida de lo razonablemente factible y, si está provisto, el Identificador Uniforme de Recursos (Uniform Resource Identifier) que el Licenciante especifica para ser asociado con la Obra, salvo que tal URI no se refiera a la nota sobre los derechos de autor o a la información sobre el licenciamiento de la Obra; y en el caso de una Obra Derivada, atribuir el crédito identificando el uso de la Obra en la Obra Derivada (v.g., "Traducción Francesa de la Obra del Autor Original," o "Guión Cinematográfico basado en la Obra original del Autor Original"). Tal crédito puede ser implementado de cualquier forma razonable; en el caso, sin embargo, de Obras Derivadas u Obras Colectivas, tal crédito aparecerá, como mínimo, donde aparece el crédito de cualquier otro autor comparable y de una manera, al menos, tan destacada como el crédito de otro autor comparable.
d.	Para evitar toda confusión, el Licenciante aclara que, cuando la obra es una composición musical:
i.	Regalías por interpretación y ejecución bajo licencias generales. El Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública o la ejecución pública digital de la obra y de recolectar, sea individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, SAYCO), las regalías por la ejecución pública o por la ejecución pública digital de la obra (por ejemplo Webcast) licenciada bajo licencias generales, si la interpretación o ejecución de la obra está primordialmente orientada por o dirigida a la obtención de una ventaja comercial o una compensación monetaria privada.
ii.	Regalías por Fonogramas. El Licenciante se reserva el derecho exclusivo de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, SAYCO), una agencia de derechos musicales o algún agente designado, las regalías por cualquier fonograma que Usted cree a partir de la obra (“versión cover”) y distribuya, en los términos del régimen de derechos de autor, si la creación o distribución de esa versión cover está primordialmente destinada o dirigida a obtener una ventaja comercial o una compensación monetaria privada.
e.	Gestión de Derechos de Autor sobre Interpretaciones y Ejecuciones Digitales (WebCasting). Para evitar toda confusión, el Licenciante aclara que, cuando la obra sea un fonograma, el Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública digital de la obra (por ejemplo, webcast) y de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, Acinpro), las regalías por la ejecución pública digital de la obra (por ejemplo, webcast), sujeta a las disposiciones aplicables del régimen de Derecho de Autor, si esta ejecución pública digital está primordialmente dirigida a obtener una ventaja comercial o una compensación monetaria privada.
5. Representaciones, Garantías y Limitaciones de Responsabilidad.
A MENOS QUE LAS PARTES LO ACORDARAN DE OTRA FORMA POR ESCRITO, EL LICENCIANTE OFRECE LA OBRA (EN EL ESTADO EN EL QUE SE ENCUENTRA) “TAL CUAL”, SIN BRINDAR GARANTÍAS DE CLASE ALGUNA RESPECTO DE LA OBRA, YA SEA EXPRESA, IMPLÍCITA, LEGAL O CUALQUIERA OTRA, INCLUYENDO, SIN LIMITARSE A ELLAS, GARANTÍAS DE TITULARIDAD, COMERCIABILIDAD, ADAPTABILIDAD O ADECUACIÓN A PROPÓSITO DETERMINADO, AUSENCIA DE INFRACCIÓN, DE AUSENCIA DE DEFECTOS LATENTES O DE OTRO TIPO, O LA PRESENCIA O AUSENCIA DE ERRORES, SEAN O NO DESCUBRIBLES (PUEDAN O NO SER ESTOS DESCUBIERTOS). ALGUNAS JURISDICCIONES NO PERMITEN LA EXCLUSIÓN DE GARANTÍAS IMPLÍCITAS, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.
6. Limitación de responsabilidad.
A MENOS QUE LO EXIJA EXPRESAMENTE LA LEY APLICABLE, EL LICENCIANTE NO SERÁ RESPONSABLE ANTE USTED POR DAÑO ALGUNO, SEA POR RESPONSABILIDAD EXTRACONTRACTUAL, PRECONTRACTUAL O CONTRACTUAL, OBJETIVA O SUBJETIVA, SE TRATE DE DAÑOS MORALES O PATRIMONIALES, DIRECTOS O INDIRECTOS, PREVISTOS O IMPREVISTOS PRODUCIDOS POR EL USO DE ESTA LICENCIA O DE LA OBRA, AUN CUANDO EL LICENCIANTE HAYA SIDO ADVERTIDO DE LA POSIBILIDAD DE DICHOS DAÑOS. ALGUNAS LEYES NO PERMITEN LA EXCLUSIÓN DE CIERTA RESPONSABILIDAD, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.
7. Término.
a.	Esta Licencia y los derechos otorgados en virtud de ella terminarán automáticamente si Usted infringe alguna condición establecida en ella. Sin embargo, los individuos o entidades que han recibido Obras Derivadas o Colectivas de Usted de conformidad con esta Licencia, no verán terminadas sus licencias, siempre que estos individuos o entidades sigan cumpliendo íntegramente las condiciones de estas licencias. Las Secciones 1, 2, 5, 6, 7, y 8 subsistirán a cualquier terminación de esta Licencia.
b.	Sujeta a las condiciones y términos anteriores, la licencia otorgada aquí es perpetua (durante el período de vigencia de los derechos de autor de la obra). No obstante lo anterior, el Licenciante se reserva el derecho a publicar y/o estrenar la Obra bajo condiciones de licencia diferentes o a dejar de distribuirla en los términos de esta Licencia en cualquier momento; en el entendido, sin embargo, que esa elección no servirá para revocar esta licencia o que deba ser otorgada , bajo los términos de esta licencia), y esta licencia continuará en pleno vigor y efecto a menos que sea terminada como se expresa atrás. La Licencia revocada continuará siendo plenamente vigente y efectiva si no se le da término en las condiciones indicadas anteriormente.
8. Varios.
a.	Cada vez que Usted distribuya o ponga a disposición pública la Obra o una Obra Colectiva, el Licenciante ofrecerá al destinatario una licencia en los mismos términos y condiciones que la licencia otorgada a Usted bajo esta Licencia.
b.	Si alguna disposición de esta Licencia resulta invalidada o no exigible, según la legislación vigente, esto no afectará ni la validez ni la aplicabilidad del resto de condiciones de esta Licencia y, sin acción adicional por parte de los sujetos de este acuerdo, aquélla se entenderá reformada lo mínimo necesario para hacer que dicha disposición sea válida y exigible.
c.	Ningún término o disposición de esta Licencia se estimará renunciada y ninguna violación de ella será consentida a menos que esa renuncia o consentimiento sea otorgado por escrito y firmado por la parte que renuncie o consienta.
d.	Esta Licencia refleja el acuerdo pleno entre las partes respecto a la Obra aquí licenciada. No hay arreglos, acuerdos o declaraciones respecto a la Obra que no estén especificados en este documento. El Licenciante no se verá limitado por ninguna disposición adicional que pueda surgir en alguna comunicación emanada de Usted. Esta Licencia no puede ser modificada sin el consentimiento mutuo por escrito del Licenciante y Usted.

 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