Structural features of the two-component system LisR/LisK suggests multiple responses for the adaptation and survival of Listeria monocytogenes

Here, we characterized the structure of the two-component regulatory system, LisR/LisK, in Listeria monocytogenes. To predict the structure of both proteins and the relationship between them, we employed several bioinformatic tools and databases. Based on our results, LisK protein is embedded in the cell membrane and its modular composition (HAMP, histidine kinase and ATPase domains) is associated with its autophosphorylation (His-266). A stimulus-response likely determines the sequential signal propagation from the bacterial cell surface to its cytoplasmic components. According to our results, LisR is a cytoplasmic protein with a receptor domain (homologous to CheY) that comprises a phosphoacceptor residue (Asp-52) and a DNA-binding domain, which may allow the transmission of a specific transcriptional response. LisR/LisK has been experimentally characterized both biochemically and functionally in other Bacilli pathophysiology; our structure-function approach may facilitate the design of suitable inhibitors.

• Autores: Arenas, Nelson E.; Gutiérrez, Andrés; Sánchez Gómez, Myriam; Salazar, Luz Mary; Reyes Montaño1, Edgar
• Tipo de recurso: Article of journal
• Fecha de publicación: 2013
• Institución: Universidad de Ciencias Aplicadas y Ambientales U.D.C.A
• Repositorio: Repositorio Institucional UDCA
• Idioma: eng
• OAI Identifier: oai:repository.udca.edu.co:11158/3022
• Acceso en línea: https://revistas.javeriana.edu.co/index.php/scientarium/article/view/4757
• Palabra clave: Listeria monocytogenes; LisR/LisK; Two-component regulatory systems; Protein histidine kinase; Listeria monocytogenes; Estructura celular; Proteínas; Membrana
• Rights: openAccess
• License: Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales