Genetic diversity in a population of creole maize (Zea mays L.) evaluated by microsatellite markers in Puerto Libertador, Córdoba

Maize a plant of Mesoamerican origin, has evolved in different microenvironments, generating the great diversity of maize that exists in the world. In order to determine the genetic di-versity of a population of Creole maize, twelve microsatellite markers were evaluated in 30 accessions, in Puerto L...

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Autores:
Pardo Perez, Enrique
Cavadía Martínez, Teodora
Herrera Vanegas, Yurany
Tipo de recurso:
Article of journal
Fecha de publicación:
2018
Institución:
Universidad de Ciencias Aplicadas y Ambientales U.D.C.A
Repositorio:
Repositorio Institucional UDCA
Idioma:
eng
OAI Identifier:
oai:repository.udca.edu.co:11158/1500
Acceso en línea:
https://revistas.udca.edu.co/index.php/ruadc/article/view/981
https://doi.org/10.31910/rudca.v21.n2.2018.981
Palabra clave:
Diversidad genética (como recurso)
Alelos
Diversidad genética
Heterocigosidad
Equilibrio Hardy-Weinberg
Alelos
Genotipos
Heterocigotos
Rights
openAccess
License
Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales, 2018
Description
Summary:Maize a plant of Mesoamerican origin, has evolved in different microenvironments, generating the great diversity of maize that exists in the world. In order to determine the genetic di-versity of a population of Creole maize, twelve microsatellite markers were evaluated in 30 accessions, in Puerto Liber-tador, Córdoba. The DNA of each accession was extracted using the PROMEGA kit, the markers were amplified by the PCR technique and the amplicons were run on polyacryl-amide gels, the gels were digitalized and the molecular sizes were determined by an exponential model. Results showed a total of 66 alleles and an average of alleles of 5.5, the expect-ed heterozygosity was 0.655, the values of the polymorphic information content (PIC) ranged from 0.352 to 0.838, with an average of 0.592 and the Hardy-Weinberg equilibrium showed imbalance (p <0.05). This work revealed that the studied accessions of Creole maize showed a high degree of polymorphism, high genetic variability and microsatellite markers were the appropriate for the evaluation of genetic diversity. This information shows to be useful for the conser-vation and protection of the genetic diversity of the studied Creole Maize.

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