Bacterias endófitas asociadas a los géneros Cyperus y Paspalum en suelos contaminados con mercurio
The objective of this study was to isolate and identify endophytic bacteria mercury resistant, associated to the genus Cyperus and Paspalum in the locality of Mine Santa Cruz, Bolivar, Colombia. Soil and herbaceous plant samples were collected to evaluate total mercury concentration in soil and plan...
- Autores:
-
Pérez, Alexander
Martínez, Dalila
Barraza, Zafiro
Marrugo, José
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2016
- Institución:
- Universidad de Ciencias Aplicadas y Ambientales U.D.C.A
- Repositorio:
- Repositorio Institucional UDCA
- Idioma:
- spa
- OAI Identifier:
- oai:repository.udca.edu.co:11158/1694
- Acceso en línea:
- https://revistas.udca.edu.co/index.php/ruadc/article/view/111
https://doi.org/10.31910/rudca.v19.n1.2016.111
- Palabra clave:
- Suelos
Minería
Metales pesados
Resistencia
Herbáceas
Cyperus
Paspalum
Mercurio
- Rights
- openAccess
- License
- Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales, 2016
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Bacterias endófitas asociadas a los géneros Cyperus y Paspalum en suelos contaminados con mercurio |
| dc.title.alternative.spa.fl_str_mv |
Endophytic bacteria associated to genus Cyperus and Paspalum in soils with mercury contamination |
| title |
Bacterias endófitas asociadas a los géneros Cyperus y Paspalum en suelos contaminados con mercurio |
| spellingShingle |
Bacterias endófitas asociadas a los géneros Cyperus y Paspalum en suelos contaminados con mercurio Suelos Minería Metales pesados Resistencia Herbáceas Cyperus Paspalum Mercurio |
| title_short |
Bacterias endófitas asociadas a los géneros Cyperus y Paspalum en suelos contaminados con mercurio |
| title_full |
Bacterias endófitas asociadas a los géneros Cyperus y Paspalum en suelos contaminados con mercurio |
| title_fullStr |
Bacterias endófitas asociadas a los géneros Cyperus y Paspalum en suelos contaminados con mercurio |
| title_full_unstemmed |
Bacterias endófitas asociadas a los géneros Cyperus y Paspalum en suelos contaminados con mercurio |
| title_sort |
Bacterias endófitas asociadas a los géneros Cyperus y Paspalum en suelos contaminados con mercurio |
| dc.creator.fl_str_mv |
Pérez, Alexander Martínez, Dalila Barraza, Zafiro Marrugo, José |
| dc.contributor.author.spa.fl_str_mv |
Pérez, Alexander Martínez, Dalila Barraza, Zafiro Marrugo, José |
| dc.subject.armarc.spa.fl_str_mv |
Suelos |
| topic |
Suelos Minería Metales pesados Resistencia Herbáceas Cyperus Paspalum Mercurio |
| dc.subject.proposal.spa.fl_str_mv |
Minería Metales pesados Resistencia Herbáceas |
| dc.subject.agrovoc.spa.fl_str_mv |
Cyperus Paspalum Mercurio |
| description |
The objective of this study was to isolate and identify endophytic bacteria mercury resistant, associated to the genus Cyperus and Paspalum in the locality of Mine Santa Cruz, Bolivar, Colombia. Soil and herbaceous plant samples were collected to evaluate total mercury concentration in soil and plant tissues, using cold-vapour atomic absorption spectrophotometry. The plant tissue samples were used for isolation and population determination of endophytic bacteria. The mercury resistance of isolates was evaluated in nutrient agar supplemented with HgCl2, the resistant isolates were identified molecularly. The total concentration of mercury in the soil of Mine Santa Cruz was 4.7mg·kg-1. Plant species collected were Paspalum arundinaceum, Cyperus luzulae and Cyperus laxus. The highest concentration of mercury in plant tissues was found in root with a range of 1.5 to 3,8mg·kg-1. The species P. arundinaceum and C. laxuspresented the greater population densities of endophytic bacteria in their tissues about 1 x 108 UFC. Seven endophytic bacteria, resistant to concentrations of 350 and 400ppm of mercury were isolated. From P. arundinaceum tissues four bacteria corresponding to Burkholderia cepacia, Burkholderia sp. and two isolates similar to Bacillussp., from C. luzulae two endophytic bacteria resistant to mercury, one identified as Ralstonia solanacearum and the other one as Burkholderia sp.; from C. laxus tissues one bacteria belonging to family Xanthomonadaceae, resistant to mercury, was isolated. |
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2016 |
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2016-01 |
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2019-06-28T20:03:13Z |
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2019-06-28T20:03:13Z |
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Artículo de revista |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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http://purl.org/coar/resource_type/c_6501 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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Text |
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0123-4226 |
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https://revistas.udca.edu.co/index.php/ruadc/article/view/111 |
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https://doi.org/10.31910/rudca.v19.n1.2016.111 |
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304504 |
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https://revistas.udca.edu.co/index.php/ruadc/article/view/111 https://doi.org/10.31910/rudca.v19.n1.2016.111 |
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spa |
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spa |
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Revista UDCA : Actualidad & Divulgación Científica (Bogotá). -- Vol. 19, No. 1 (Ene.-Jun. 2016). -- páginas 67-76 |
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Agricultura |
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Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales, 2016 |
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info:eu-repo/semantics/openAccess |
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Atribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0) |
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Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales, 2016 https://creativecommons.org/licenses/by-nc-sa/4.0/ Atribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0) http://purl.org/coar/access_right/c_abf2 |
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Bogotá : Universidad de Ciencias Aplicadas y Ambientales, 2016 |
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Pérez, AlexanderMartínez, DalilaBarraza, ZafiroMarrugo, José2019-06-28T20:03:13Z2019-06-28T20:03:13Z2016-010123-4226https://revistas.udca.edu.co/index.php/ruadc/article/view/111https://doi.org/10.31910/rudca.v19.n1.2016.111304504The objective of this study was to isolate and identify endophytic bacteria mercury resistant, associated to the genus Cyperus and Paspalum in the locality of Mine Santa Cruz, Bolivar, Colombia. Soil and herbaceous plant samples were collected to evaluate total mercury concentration in soil and plant tissues, using cold-vapour atomic absorption spectrophotometry. The plant tissue samples were used for isolation and population determination of endophytic bacteria. The mercury resistance of isolates was evaluated in nutrient agar supplemented with HgCl2, the resistant isolates were identified molecularly. The total concentration of mercury in the soil of Mine Santa Cruz was 4.7mg·kg-1. Plant species collected were Paspalum arundinaceum, Cyperus luzulae and Cyperus laxus. The highest concentration of mercury in plant tissues was found in root with a range of 1.5 to 3,8mg·kg-1. The species P. arundinaceum and C. laxuspresented the greater population densities of endophytic bacteria in their tissues about 1 x 108 UFC. Seven endophytic bacteria, resistant to concentrations of 350 and 400ppm of mercury were isolated. From P. arundinaceum tissues four bacteria corresponding to Burkholderia cepacia, Burkholderia sp. and two isolates similar to Bacillussp., from C. luzulae two endophytic bacteria resistant to mercury, one identified as Ralstonia solanacearum and the other one as Burkholderia sp.; from C. laxus tissues one bacteria belonging to family Xanthomonadaceae, resistant to mercury, was isolated.El objetivo de este estudio fue aislar e identificar bacterias endófitas resistentes a mercurio, asociadas a los géneros Cyperus y Paspalum, en suelos de Mina Santa Cruz, Bolívar, Colombia. Para ello, se colectaron muestras de suelo y de plantas herbáceas, con el fin de evaluar, mediante espectrofotometría de absorción atómica por vapor frío, la concentración de mercurio total. Se utilizaron muestras de tejidos vegetales para el aislamiento y la determinación poblacional de bacterias endófitas. La resistencia a mercurio in vitro de los aislados, se evaluó en agar nutritivo suplementado con HgCl2; los aislados resistentes fueron identificados, a nivel molecular. La concentración de mercurio total en el suelo de Mina Santa Cruz fue de 4,7mg·kg-1. Las especies vegetales colectadas fueron Paspalum arundinaceum, Cyperus luzulae y Cyperus laxus. La mayor concentración de mercurio en los tejidos vegetales fue encontrada en raíz, con un rango de 1,5 a 3,8mg·kg-1. Las especies P. arundinaceum y C. laxus presentaron las mayores densidades poblacionales de endófitas en sus tejidos 1 x 108 UFC, aproximadamente. Se aislaron siete bacterias endófitas resistentes, a concentraciones de 350 y 400 ppm de mercurio. De los tejidos de P. arundinaceum, se aislaron cuatro bacterias, que corresponden a Burkholderia cepacia, Burkholderia sp. y dos aislados tuvieron gran similitud con Bacillus sp.; para C. luzulae, se aislaron dos endófitas resistentes a mercurio, una identificada como Ralstonia solanacearum y, la otra, como Burkholderia sp.; para C. laxus, un aislado que hace parte de la familia Xanthomonadaceae, también tuvo resistencia a mercurio.Incluye referencias bibliográficasapplication/pdfspaBogotá : Universidad de Ciencias Aplicadas y Ambientales, 2016Revista UDCA : Actualidad & Divulgación Científica (Bogotá). -- Vol. 19, No. 1 (Ene.-Jun. 2016). -- páginas 67-76AgriculturaDerechos Reservados - Universidad de Ciencias Aplicadas y Ambientales, 2016https://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccessAtribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)http://purl.org/coar/access_right/c_abf2Bacterias endófitas asociadas a los géneros Cyperus y Paspalum en suelos contaminados con mercurioEndophytic bacteria associated to genus Cyperus and Paspalum in soils with mercury contaminationArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionTexthttp://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85SuelosMineríaMetales pesadosResistenciaHerbáceasCyperusPaspalumMercurioPublicationORIGINAL111-Texto del artículo-4309-1-10-20180920.pdf111-Texto del artículo-4309-1-10-20180920.pdfapplication/pdf368052https://repository.udca.edu.co/bitstreams/a5a86eb8-72fc-4c20-b7b8-ee3f192f6fa5/download844f21a9f0808dadf8fdd4c7fde47334MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-814775https://repository.udca.edu.co/bitstreams/d0168bdc-bd90-413e-8e81-e7f43a0520e0/downloadf661acf14bedbf9f5d13897a0387e751MD52TEXT111-Texto del artículo-4309-1-10-20180920.pdf.txt111-Texto del 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en las Obras Colectivas;
b.	Distribuir copias o fonogramas de las Obras, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública, incluyéndolas como incorporadas en Obras Colectivas, según corresponda;
c.	Distribuir copias de las Obras Derivadas que se generen, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública.
Los derechos mencionados anteriormente pueden ser ejercidos en todos los medios y formatos, actualmente conocidos o que se inventen en el futuro. Los derechos antes mencionados incluyen el derecho a realizar dichas modificaciones en la medida que sean técnicamente necesarias para ejercer los derechos en otro medio o formatos, pero de otra manera usted no está autorizado para realizar obras derivadas.Todos los derechos no otorgados expresamente por el Licenciante quedan por este medio reservados, incluyendo pero sin limitarse a aquellos que se mencionan en las secciones 4(d) y 4(e).
4. Restricciones.
La licencia otorgada en la anterior Sección 3 está expresamente sujeta y limitada por las siguientes restricciones:
a.	Usted puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra sólo bajo las condiciones de esta Licencia, y Usted debe incluir una copia de esta licencia o del Identificador Universal de Recursos de la misma con cada copia de la Obra que distribuya, exhiba públicamente, ejecute públicamente o ponga a disposición pública. No es posible ofrecer o imponer ninguna condición sobre la Obra que altere o limite las condiciones de esta Licencia o el ejercicio de los derechos de los destinatarios otorgados en este documento. No es posible sublicenciar la Obra. Usted debe mantener intactos todos los avisos que hagan referencia a esta Licencia y a la cláusula de limitación de garantías. Usted no puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra con alguna medida tecnológica que controle el acceso o la utilización de ella de una forma que sea inconsistente con las condiciones de esta Licencia. Lo anterior se aplica a la Obra incorporada a una Obra Colectiva, pero esto no exige que la Obra Colectiva aparte de la obra misma quede sujeta a las condiciones de esta Licencia. Si Usted crea una Obra Colectiva, previo aviso de cualquier Licenciante debe, en la medida de lo posible, eliminar de la Obra Colectiva cualquier referencia a dicho Licenciante o al Autor Original, según lo solicitado por el Licenciante y conforme lo exige la cláusula 4(c).
b.	Usted no puede ejercer ninguno de los derechos que le han sido otorgados en la Sección 3 precedente de modo que estén principalmente destinados o directamente dirigidos a conseguir un provecho comercial o una compensación monetaria privada. El intercambio de la Obra por otras obras protegidas por derechos de autor, ya sea a través de un sistema para compartir archivos digitales (digital file-sharing) o de cualquier otra manera no será considerado como estar destinado principalmente o dirigido directamente a conseguir un provecho comercial o una compensación monetaria privada, siempre que no se realice un pago mediante una compensación monetaria en relación con el intercambio de obras protegidas por el derecho de autor.
c.	Si usted distribuye, exhibe públicamente, ejecuta públicamente o ejecuta públicamente en forma digital la Obra o cualquier Obra Derivada u Obra Colectiva, Usted debe mantener intacta toda la información de derecho de autor de la Obra y proporcionar, de forma razonable según el medio o manera que Usted esté utilizando: (i) el nombre del Autor Original si está provisto (o seudónimo, si fuere aplicable), y/o (ii) el nombre de la parte o las partes que el Autor Original y/o el Licenciante hubieren designado para la atribución (v.g., un instituto patrocinador, editorial, publicación) en la información de los derechos de autor del Licenciante, términos de servicios o de otras formas razonables; el título de la Obra si está provisto; en la medida de lo razonablemente factible y, si está provisto, el Identificador Uniforme de Recursos (Uniform Resource Identifier) que el Licenciante especifica para ser asociado con la Obra, salvo que tal URI no se refiera a la nota sobre los derechos de autor o a la información sobre el licenciamiento de la Obra; y en el caso de una Obra Derivada, atribuir el crédito identificando el uso de la Obra en la Obra Derivada (v.g., "Traducción Francesa de la Obra del Autor Original," o "Guión Cinematográfico basado en la Obra original del Autor Original"). Tal crédito puede ser implementado de cualquier forma razonable; en el caso, sin embargo, de Obras Derivadas u Obras Colectivas, tal crédito aparecerá, como mínimo, donde aparece el crédito de cualquier otro autor comparable y de una manera, al menos, tan destacada como el crédito de otro autor comparable.
d.	Para evitar toda confusión, el Licenciante aclara que, cuando la obra es una composición musical:
i.	Regalías por interpretación y ejecución bajo licencias generales. El Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública o la ejecución pública digital de la obra y de recolectar, sea individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, SAYCO), las regalías por la ejecución pública o por la ejecución pública digital de la obra (por ejemplo Webcast) licenciada bajo licencias generales, si la interpretación o ejecución de la obra está primordialmente orientada por o dirigida a la obtención de una ventaja comercial o una compensación monetaria privada.
ii.	Regalías por Fonogramas. El Licenciante se reserva el derecho exclusivo de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, SAYCO), una agencia de derechos musicales o algún agente designado, las regalías por cualquier fonograma que Usted cree a partir de la obra (“versión cover”) y distribuya, en los términos del régimen de derechos de autor, si la creación o distribución de esa versión cover está primordialmente destinada o dirigida a obtener una ventaja comercial o una compensación monetaria privada.
e.	Gestión de Derechos de Autor sobre Interpretaciones y Ejecuciones Digitales (WebCasting). Para evitar toda confusión, el Licenciante aclara que, cuando la obra sea un fonograma, el Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública digital de la obra (por ejemplo, webcast) y de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, Acinpro), las regalías por la ejecución pública digital de la obra (por ejemplo, webcast), sujeta a las disposiciones aplicables del régimen de Derecho de Autor, si esta ejecución pública digital está primordialmente dirigida a obtener una ventaja comercial o una compensación monetaria privada.
5. Representaciones, Garantías y Limitaciones de Responsabilidad.
A MENOS QUE LAS PARTES LO ACORDARAN DE OTRA FORMA POR ESCRITO, EL LICENCIANTE OFRECE LA OBRA (EN EL ESTADO EN EL QUE SE ENCUENTRA) “TAL CUAL”, SIN BRINDAR GARANTÍAS DE CLASE ALGUNA RESPECTO DE LA OBRA, YA SEA EXPRESA, IMPLÍCITA, LEGAL O CUALQUIERA OTRA, INCLUYENDO, SIN LIMITARSE A ELLAS, GARANTÍAS DE TITULARIDAD, COMERCIABILIDAD, ADAPTABILIDAD O ADECUACIÓN A PROPÓSITO DETERMINADO, AUSENCIA DE INFRACCIÓN, DE AUSENCIA DE DEFECTOS LATENTES O DE OTRO TIPO, O LA PRESENCIA O AUSENCIA DE ERRORES, SEAN O NO DESCUBRIBLES (PUEDAN O NO SER ESTOS DESCUBIERTOS). ALGUNAS JURISDICCIONES NO PERMITEN LA EXCLUSIÓN DE GARANTÍAS IMPLÍCITAS, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.
6. Limitación de responsabilidad.
A MENOS QUE LO EXIJA EXPRESAMENTE LA LEY APLICABLE, EL LICENCIANTE NO SERÁ RESPONSABLE ANTE USTED POR DAÑO ALGUNO, SEA POR RESPONSABILIDAD EXTRACONTRACTUAL, PRECONTRACTUAL O CONTRACTUAL, OBJETIVA O SUBJETIVA, SE TRATE DE DAÑOS MORALES O PATRIMONIALES, DIRECTOS O INDIRECTOS, PREVISTOS O IMPREVISTOS PRODUCIDOS POR EL USO DE ESTA LICENCIA O DE LA OBRA, AUN CUANDO EL LICENCIANTE HAYA SIDO ADVERTIDO DE LA POSIBILIDAD DE DICHOS DAÑOS. ALGUNAS LEYES NO PERMITEN LA EXCLUSIÓN DE CIERTA RESPONSABILIDAD, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.
7. Término.
a.	Esta Licencia y los derechos otorgados en virtud de ella terminarán automáticamente si Usted infringe alguna condición establecida en ella. Sin embargo, los individuos o entidades que han recibido Obras Derivadas o Colectivas de Usted de conformidad con esta Licencia, no verán terminadas sus licencias, siempre que estos individuos o entidades sigan cumpliendo íntegramente las condiciones de estas licencias. Las Secciones 1, 2, 5, 6, 7, y 8 subsistirán a cualquier terminación de esta Licencia.
b.	Sujeta a las condiciones y términos anteriores, la licencia otorgada aquí es perpetua (durante el período de vigencia de los derechos de autor de la obra). No obstante lo anterior, el Licenciante se reserva el derecho a publicar y/o estrenar la Obra bajo condiciones de licencia diferentes o a dejar de distribuirla en los términos de esta Licencia en cualquier momento; en el entendido, sin embargo, que esa elección no servirá para revocar esta licencia o que deba ser otorgada , bajo los términos de esta licencia), y esta licencia continuará en pleno vigor y efecto a menos que sea terminada como se expresa atrás. La Licencia revocada continuará siendo plenamente vigente y efectiva si no se le da término en las condiciones indicadas anteriormente.
8. Varios.
a.	Cada vez que Usted distribuya o ponga a disposición pública la Obra o una Obra Colectiva, el Licenciante ofrecerá al destinatario una licencia en los mismos términos y condiciones que la licencia otorgada a Usted bajo esta Licencia.
b.	Si alguna disposición de esta Licencia resulta invalidada o no exigible, según la legislación vigente, esto no afectará ni la validez ni la aplicabilidad del resto de condiciones de esta Licencia y, sin acción adicional por parte de los sujetos de este acuerdo, aquélla se entenderá reformada lo mínimo necesario para hacer que dicha disposición sea válida y exigible.
c.	Ningún término o disposición de esta Licencia se estimará renunciada y ninguna violación de ella será consentida a menos que esa renuncia o consentimiento sea otorgado por escrito y firmado por la parte que renuncie o consienta.
d.	Esta Licencia refleja el acuerdo pleno entre las partes respecto a la Obra aquí licenciada. No hay arreglos, acuerdos o declaraciones respecto a la Obra que no estén especificados en este documento. El Licenciante no se verá limitado por ninguna disposición adicional que pueda surgir en alguna comunicación emanada de Usted. Esta Licencia no puede ser modificada sin el consentimiento mutuo por escrito del Licenciante y Usted.

 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