Comparación de métodos de inoculación de Fusarium solani f. sp. passiflorae en plántulas de maracuyá (Passiflora edulis f. flavicarpa)

Passionfruit is a species susceptible pathogens such as Fusarium solani f. sp. passiflorae, causal agent of Passiflora collar rot, which can cause yield losses between 90 and 100% in commercial crops in Colombia. Due to lack of knowledge of an efficient inoculation method to evaluate germplasm of pa...

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Autores:
Ángel García, Carolina
Robledo Buriticá, Jacobo
Castaño Zapata, Jairo
Tipo de recurso:
Article of journal
Fecha de publicación:
2018
Institución:
Universidad de Ciencias Aplicadas y Ambientales U.D.C.A
Repositorio:
Repositorio Institucional UDCA
Idioma:
spa
OAI Identifier:
oai:repository.udca.edu.co:11158/1398
Acceso en línea:
https://revistas.udca.edu.co/index.php/ruadc/article/view/659
https://doi.org/10.31910/rudca.v21.n1.2018.659
Palabra clave:
Patogenicidad
Maracuyá - Enfermedades y plagas
Nectria haematococca
Colonización
Incubación de enfermedades
Patogenicidad
Maracuyá (Passiflora edulis)
Rights
openAccess
License
Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales, 2018
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dc.title.spa.fl_str_mv Comparación de métodos de inoculación de Fusarium solani f. sp. passiflorae en plántulas de maracuyá (Passiflora edulis f. flavicarpa)
dc.title.alternative.eng.fl_str_mv Comparison of methods of inoculation of Fusarium solani f. sp. passiflorae in seedlings of passion fruit (Passiflora edulis f. flavicarpa)
title Comparación de métodos de inoculación de Fusarium solani f. sp. passiflorae en plántulas de maracuyá (Passiflora edulis f. flavicarpa)
spellingShingle Comparación de métodos de inoculación de Fusarium solani f. sp. passiflorae en plántulas de maracuyá (Passiflora edulis f. flavicarpa)
Patogenicidad
Maracuyá - Enfermedades y plagas
Nectria haematococca
Colonización
Incubación de enfermedades
Patogenicidad
Maracuyá (Passiflora edulis)
title_short Comparación de métodos de inoculación de Fusarium solani f. sp. passiflorae en plántulas de maracuyá (Passiflora edulis f. flavicarpa)
title_full Comparación de métodos de inoculación de Fusarium solani f. sp. passiflorae en plántulas de maracuyá (Passiflora edulis f. flavicarpa)
title_fullStr Comparación de métodos de inoculación de Fusarium solani f. sp. passiflorae en plántulas de maracuyá (Passiflora edulis f. flavicarpa)
title_full_unstemmed Comparación de métodos de inoculación de Fusarium solani f. sp. passiflorae en plántulas de maracuyá (Passiflora edulis f. flavicarpa)
title_sort Comparación de métodos de inoculación de Fusarium solani f. sp. passiflorae en plántulas de maracuyá (Passiflora edulis f. flavicarpa)
dc.creator.fl_str_mv Ángel García, Carolina
Robledo Buriticá, Jacobo
Castaño Zapata, Jairo
dc.contributor.author.spa.fl_str_mv Ángel García, Carolina
Robledo Buriticá, Jacobo
Castaño Zapata, Jairo
dc.subject.lemb.spa.fl_str_mv Patogenicidad
Maracuyá - Enfermedades y plagas
topic Patogenicidad
Maracuyá - Enfermedades y plagas
Nectria haematococca
Colonización
Incubación de enfermedades
Patogenicidad
Maracuyá (Passiflora edulis)
dc.subject.proposal.spa.fl_str_mv Nectria haematococca
Colonización
Incubación de enfermedades
Patogenicidad
dc.subject.agrovoc.spa.fl_str_mv Maracuyá (Passiflora edulis)
description Passionfruit is a species susceptible pathogens such as Fusarium solani f. sp. passiflorae, causal agent of Passiflora collar rot, which can cause yield losses between 90 and 100% in commercial crops in Colombia. Due to lack of knowledge of an efficient inoculation method to evaluate germplasm of passionfruit against this fungus, it was designed this study, whose objective was to compare four methods of inoculation of F. solani f. sp. passiflorae MViRi01 in passionfruit seedlings (Passiflora edulis f. flavicarpa) cultivated at 29ºC and 12-hours of photoperiod. The treatments were: stem incision (T1), root immersion (T2), stem injection (T3), and a modified test-tube screening methodology (T4). Disease incidence and incubation period of the fungus was evaluated. The assessed methods showed highly significant differences (P< 0.001). F. solani f. sp. passiflorae infected roots, stem, and leaves causing stem ascending and descending strangulation. Both T1 (incision) and T4 (screening) treatments showed 100% of disease incidence. The incubation period of the fungus in T4 was 2.4 days and in T2 (immersion) 19 days. The death of the seedlings occurred 12 days after inoculation in T1 and T3 (injection) treatments. The adventitious root sprouting in the seedlings was induced by injecting (T3) the pathogen. The most suitable methods to inoculate F. solani f. sp. passiflorae on passionfruit seedlings were the modified testtube screening methodology and the stem incision due to its homogeneity and replicability results.
publishDate 2018
dc.date.issued.spa.fl_str_mv 2018-01
dc.date.accessioned.spa.fl_str_mv 2019-05-10T20:15:05Z
dc.date.available.spa.fl_str_mv 2019-05-10T20:15:05Z
dc.type.spa.fl_str_mv Artículo de revista
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url https://revistas.udca.edu.co/index.php/ruadc/article/view/659
https://doi.org/10.31910/rudca.v21.n1.2018.659
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dc.relation.ispartofseries.spa.fl_str_mv Revista UDCA : Actualidad & Divulgación Científica (Bogotá). -- Vol. 21, No. 1 (Ene.-Jun. 2018). -- páginas 23-31
dc.relation.indexed.spa.fl_str_mv Agricultura
dc.rights.spa.fl_str_mv Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales, 2018
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spelling Ángel García, CarolinaRobledo Buriticá, JacoboCastaño Zapata, Jairo2019-05-10T20:15:05Z2019-05-10T20:15:05Z2018-010123-4226https://revistas.udca.edu.co/index.php/ruadc/article/view/659https://doi.org/10.31910/rudca.v21.n1.2018.659307048Passionfruit is a species susceptible pathogens such as Fusarium solani f. sp. passiflorae, causal agent of Passiflora collar rot, which can cause yield losses between 90 and 100% in commercial crops in Colombia. Due to lack of knowledge of an efficient inoculation method to evaluate germplasm of passionfruit against this fungus, it was designed this study, whose objective was to compare four methods of inoculation of F. solani f. sp. passiflorae MViRi01 in passionfruit seedlings (Passiflora edulis f. flavicarpa) cultivated at 29ºC and 12-hours of photoperiod. The treatments were: stem incision (T1), root immersion (T2), stem injection (T3), and a modified test-tube screening methodology (T4). Disease incidence and incubation period of the fungus was evaluated. The assessed methods showed highly significant differences (P< 0.001). F. solani f. sp. passiflorae infected roots, stem, and leaves causing stem ascending and descending strangulation. Both T1 (incision) and T4 (screening) treatments showed 100% of disease incidence. The incubation period of the fungus in T4 was 2.4 days and in T2 (immersion) 19 days. The death of the seedlings occurred 12 days after inoculation in T1 and T3 (injection) treatments. The adventitious root sprouting in the seedlings was induced by injecting (T3) the pathogen. The most suitable methods to inoculate F. solani f. sp. passiflorae on passionfruit seedlings were the modified testtube screening methodology and the stem incision due to its homogeneity and replicability results.El maracuyá es una especie susceptible a patógenos, como Fusarium solani f . sp . passiflorae, causante de la secadera, llegando a causar pérdidas entre 90 y 100%, en cultivos comerciales en Colombia . Debido al desconocimiento de un método de inoculación eficiente para evaluar germoplasma de maracuyá contra este patógeno, se planteó este estudio, cuyo objetivo fue comparar cuatro métodos de inoculación de F. solani f . sp . passiflorae MViRi01 en plántulas de maracuyá (Passiflora edulis f . flavicarpa), cultivadas a 29°C, con un fotoperiodo de 12h . Los tratamientos fueron: incisión en el tallo (T1), inmersión de raíces (T2), inyección en el tallo (T3) y cribado en tubos de ensayo modificado (T4) . Se estimó la incidencia de la enfermedad y el periodo de incubación del hongo . Los métodos evaluados mostraron diferencias altamente significativas (P<0,001) . F. solani f . sp . passiflorae infectó raíces, tallo y hojas, causando estrangulamiento ascendente y descendente del tallo . Se obtuvo 100% de incidencia de la enfermedad con los tratamientos T1 (incisión) y T4 (cribado) . En T4, el periodo de incubación del hongo fue 2,4 días y en T2 (inmersión), de 19 días . La muerte de las plántulas ocurrió 12 días después de inocular los tratamientos T1 y T3 (inyección) . La emisión de raíces adventicias en las plántulas se indujo inyectando el patógeno (T3) . Los métodos apropiados para inocular Fusarium sp . en plántulas de maracuyá fueron el cribado en tubos de ensayo modificado y la incisión en el tallo, debido a la homogeneidad en los resultados y la replicabilidad.Incluye referencias bibliográficasapplication/pdfspaRevista UDCA : Actualidad & Divulgación Científica (Bogotá). -- Vol. 21, No. 1 (Ene.-Jun. 2018). -- páginas 23-31AgriculturaDerechos Reservados - Universidad de Ciencias Aplicadas y Ambientales, 2018https://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccessAtribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)http://purl.org/coar/access_right/c_abf2Comparación de métodos de inoculación de Fusarium solani f. sp. passiflorae en plántulas de maracuyá (Passiflora edulis f. flavicarpa)Comparison of methods of inoculation of Fusarium solani f. sp. passiflorae in seedlings of passion fruit (Passiflora edulis f. flavicarpa)Artículo de 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en las Obras Colectivas;
b.	Distribuir copias o fonogramas de las Obras, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública, incluyéndolas como incorporadas en Obras Colectivas, según corresponda;
c.	Distribuir copias de las Obras Derivadas que se generen, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública.
Los derechos mencionados anteriormente pueden ser ejercidos en todos los medios y formatos, actualmente conocidos o que se inventen en el futuro. Los derechos antes mencionados incluyen el derecho a realizar dichas modificaciones en la medida que sean técnicamente necesarias para ejercer los derechos en otro medio o formatos, pero de otra manera usted no está autorizado para realizar obras derivadas.Todos los derechos no otorgados expresamente por el Licenciante quedan por este medio reservados, incluyendo pero sin limitarse a aquellos que se mencionan en las secciones 4(d) y 4(e).
4. Restricciones.
La licencia otorgada en la anterior Sección 3 está expresamente sujeta y limitada por las siguientes restricciones:
a.	Usted puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra sólo bajo las condiciones de esta Licencia, y Usted debe incluir una copia de esta licencia o del Identificador Universal de Recursos de la misma con cada copia de la Obra que distribuya, exhiba públicamente, ejecute públicamente o ponga a disposición pública. No es posible ofrecer o imponer ninguna condición sobre la Obra que altere o limite las condiciones de esta Licencia o el ejercicio de los derechos de los destinatarios otorgados en este documento. No es posible sublicenciar la Obra. Usted debe mantener intactos todos los avisos que hagan referencia a esta Licencia y a la cláusula de limitación de garantías. Usted no puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra con alguna medida tecnológica que controle el acceso o la utilización de ella de una forma que sea inconsistente con las condiciones de esta Licencia. Lo anterior se aplica a la Obra incorporada a una Obra Colectiva, pero esto no exige que la Obra Colectiva aparte de la obra misma quede sujeta a las condiciones de esta Licencia. Si Usted crea una Obra Colectiva, previo aviso de cualquier Licenciante debe, en la medida de lo posible, eliminar de la Obra Colectiva cualquier referencia a dicho Licenciante o al Autor Original, según lo solicitado por el Licenciante y conforme lo exige la cláusula 4(c).
b.	Usted no puede ejercer ninguno de los derechos que le han sido otorgados en la Sección 3 precedente de modo que estén principalmente destinados o directamente dirigidos a conseguir un provecho comercial o una compensación monetaria privada. El intercambio de la Obra por otras obras protegidas por derechos de autor, ya sea a través de un sistema para compartir archivos digitales (digital file-sharing) o de cualquier otra manera no será considerado como estar destinado principalmente o dirigido directamente a conseguir un provecho comercial o una compensación monetaria privada, siempre que no se realice un pago mediante una compensación monetaria en relación con el intercambio de obras protegidas por el derecho de autor.
c.	Si usted distribuye, exhibe públicamente, ejecuta públicamente o ejecuta públicamente en forma digital la Obra o cualquier Obra Derivada u Obra Colectiva, Usted debe mantener intacta toda la información de derecho de autor de la Obra y proporcionar, de forma razonable según el medio o manera que Usted esté utilizando: (i) el nombre del Autor Original si está provisto (o seudónimo, si fuere aplicable), y/o (ii) el nombre de la parte o las partes que el Autor Original y/o el Licenciante hubieren designado para la atribución (v.g., un instituto patrocinador, editorial, publicación) en la información de los derechos de autor del Licenciante, términos de servicios o de otras formas razonables; el título de la Obra si está provisto; en la medida de lo razonablemente factible y, si está provisto, el Identificador Uniforme de Recursos (Uniform Resource Identifier) que el Licenciante especifica para ser asociado con la Obra, salvo que tal URI no se refiera a la nota sobre los derechos de autor o a la información sobre el licenciamiento de la Obra; y en el caso de una Obra Derivada, atribuir el crédito identificando el uso de la Obra en la Obra Derivada (v.g., "Traducción Francesa de la Obra del Autor Original," o "Guión Cinematográfico basado en la Obra original del Autor Original"). Tal crédito puede ser implementado de cualquier forma razonable; en el caso, sin embargo, de Obras Derivadas u Obras Colectivas, tal crédito aparecerá, como mínimo, donde aparece el crédito de cualquier otro autor comparable y de una manera, al menos, tan destacada como el crédito de otro autor comparable.
d.	Para evitar toda confusión, el Licenciante aclara que, cuando la obra es una composición musical:
i.	Regalías por interpretación y ejecución bajo licencias generales. El Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública o la ejecución pública digital de la obra y de recolectar, sea individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, SAYCO), las regalías por la ejecución pública o por la ejecución pública digital de la obra (por ejemplo Webcast) licenciada bajo licencias generales, si la interpretación o ejecución de la obra está primordialmente orientada por o dirigida a la obtención de una ventaja comercial o una compensación monetaria privada.
ii.	Regalías por Fonogramas. El Licenciante se reserva el derecho exclusivo de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, SAYCO), una agencia de derechos musicales o algún agente designado, las regalías por cualquier fonograma que Usted cree a partir de la obra (“versión cover”) y distribuya, en los términos del régimen de derechos de autor, si la creación o distribución de esa versión cover está primordialmente destinada o dirigida a obtener una ventaja comercial o una compensación monetaria privada.
e.	Gestión de Derechos de Autor sobre Interpretaciones y Ejecuciones Digitales (WebCasting). Para evitar toda confusión, el Licenciante aclara que, cuando la obra sea un fonograma, el Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública digital de la obra (por ejemplo, webcast) y de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, Acinpro), las regalías por la ejecución pública digital de la obra (por ejemplo, webcast), sujeta a las disposiciones aplicables del régimen de Derecho de Autor, si esta ejecución pública digital está primordialmente dirigida a obtener una ventaja comercial o una compensación monetaria privada.
5. Representaciones, Garantías y Limitaciones de Responsabilidad.
A MENOS QUE LAS PARTES LO ACORDARAN DE OTRA FORMA POR ESCRITO, EL LICENCIANTE OFRECE LA OBRA (EN EL ESTADO EN EL QUE SE ENCUENTRA) “TAL CUAL”, SIN BRINDAR GARANTÍAS DE CLASE ALGUNA RESPECTO DE LA OBRA, YA SEA EXPRESA, IMPLÍCITA, LEGAL O CUALQUIERA OTRA, INCLUYENDO, SIN LIMITARSE A ELLAS, GARANTÍAS DE TITULARIDAD, COMERCIABILIDAD, ADAPTABILIDAD O ADECUACIÓN A PROPÓSITO DETERMINADO, AUSENCIA DE INFRACCIÓN, DE AUSENCIA DE DEFECTOS LATENTES O DE OTRO TIPO, O LA PRESENCIA O AUSENCIA DE ERRORES, SEAN O NO DESCUBRIBLES (PUEDAN O NO SER ESTOS DESCUBIERTOS). ALGUNAS JURISDICCIONES NO PERMITEN LA EXCLUSIÓN DE GARANTÍAS IMPLÍCITAS, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.
6. Limitación de responsabilidad.
A MENOS QUE LO EXIJA EXPRESAMENTE LA LEY APLICABLE, EL LICENCIANTE NO SERÁ RESPONSABLE ANTE USTED POR DAÑO ALGUNO, SEA POR RESPONSABILIDAD EXTRACONTRACTUAL, PRECONTRACTUAL O CONTRACTUAL, OBJETIVA O SUBJETIVA, SE TRATE DE DAÑOS MORALES O PATRIMONIALES, DIRECTOS O INDIRECTOS, PREVISTOS O IMPREVISTOS PRODUCIDOS POR EL USO DE ESTA LICENCIA O DE LA OBRA, AUN CUANDO EL LICENCIANTE HAYA SIDO ADVERTIDO DE LA POSIBILIDAD DE DICHOS DAÑOS. ALGUNAS LEYES NO PERMITEN LA EXCLUSIÓN DE CIERTA RESPONSABILIDAD, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.
7. Término.
a.	Esta Licencia y los derechos otorgados en virtud de ella terminarán automáticamente si Usted infringe alguna condición establecida en ella. Sin embargo, los individuos o entidades que han recibido Obras Derivadas o Colectivas de Usted de conformidad con esta Licencia, no verán terminadas sus licencias, siempre que estos individuos o entidades sigan cumpliendo íntegramente las condiciones de estas licencias. Las Secciones 1, 2, 5, 6, 7, y 8 subsistirán a cualquier terminación de esta Licencia.
b.	Sujeta a las condiciones y términos anteriores, la licencia otorgada aquí es perpetua (durante el período de vigencia de los derechos de autor de la obra). No obstante lo anterior, el Licenciante se reserva el derecho a publicar y/o estrenar la Obra bajo condiciones de licencia diferentes o a dejar de distribuirla en los términos de esta Licencia en cualquier momento; en el entendido, sin embargo, que esa elección no servirá para revocar esta licencia o que deba ser otorgada , bajo los términos de esta licencia), y esta licencia continuará en pleno vigor y efecto a menos que sea terminada como se expresa atrás. La Licencia revocada continuará siendo plenamente vigente y efectiva si no se le da término en las condiciones indicadas anteriormente.
8. Varios.
a.	Cada vez que Usted distribuya o ponga a disposición pública la Obra o una Obra Colectiva, el Licenciante ofrecerá al destinatario una licencia en los mismos términos y condiciones que la licencia otorgada a Usted bajo esta Licencia.
b.	Si alguna disposición de esta Licencia resulta invalidada o no exigible, según la legislación vigente, esto no afectará ni la validez ni la aplicabilidad del resto de condiciones de esta Licencia y, sin acción adicional por parte de los sujetos de este acuerdo, aquélla se entenderá reformada lo mínimo necesario para hacer que dicha disposición sea válida y exigible.
c.	Ningún término o disposición de esta Licencia se estimará renunciada y ninguna violación de ella será consentida a menos que esa renuncia o consentimiento sea otorgado por escrito y firmado por la parte que renuncie o consienta.
d.	Esta Licencia refleja el acuerdo pleno entre las partes respecto a la Obra aquí licenciada. No hay arreglos, acuerdos o declaraciones respecto a la Obra que no estén especificados en este documento. El Licenciante no se verá limitado por ninguna disposición adicional que pueda surgir en alguna comunicación emanada de Usted. Esta Licencia no puede ser modificada sin el consentimiento mutuo por escrito del Licenciante y Usted.

