Generalidades de la producción de embriones bovinos in vitro
In vitro embryo production (IEP) is a reproducti- ve biotechnology that impacts livestock systems favoring breeding and which have also been uti- lized as research models for embryo development in other species. For the development of this method, oocytes in vivo are collected through laparoscopy an...
- Autores:
-
Gonella Diaza, Ángela María
Atuesta Bustos, Jorge Eduardo
Bernal Ulloa, Sandra Milena
Chacón Jaramillo, Liliana
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2013
- Institución:
- Universidad de Ciencias Aplicadas y Ambientales U.D.C.A
- Repositorio:
- Repositorio Institucional UDCA
- Idioma:
- spa
- OAI Identifier:
- oai:repository.udca.edu.co:11158/3484
- Acceso en línea:
- https://dialnet.unirioja.es/servlet/articulo?codigo=6285691
- Palabra clave:
- Biotecnología
Espermatozoides
Oocitos
Desarrollo Embrionario
Biotechnology
Embryonic develop- ment
Spermatozoids
Fertilization
Oocytes
- Rights
- openAccess
- License
- Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales
| Summary: | In vitro embryo production (IEP) is a reproducti- ve biotechnology that impacts livestock systems favoring breeding and which have also been uti- lized as research models for embryo development in other species. For the development of this method, oocytes in vivo are collected through laparoscopy and ultrasound guided follicular aspiration, or through ovaries collected from slaughterhouses. The oocytes are subjected to maturation processes in vitro using various cul- ture media, addition of gonadotropins and growth factors. Spermatozoids undergo in vitro sperm ca- pacitacion process, where fertilization capacity is adquired and live spermatozoids are separa- ted from the seminal components and Cryopro- tectants. The oocyte–sperm co-culture for the in vitro fertilization is carried out in a media that promotes maximum sperm activity and zona pe- llucida penetration. For in vitro fertilization is ca- rried out in a medium favorable to sperm activity and pellucid zone penetration. Subsequently, the collected embryos are subjected to culture media that contain energy sources, amino acids and growth factors in order to maintain their sur- vival and development. Currently, IEP in cattle has become large-scale and is working on stan- dardization process for other species. Through PIV, biotechnologies such as cloning and trans- genesis have been developed. The aim of this review is to describe the PIV phases and their relation with the physiological events of fertiliza- tion and early embryo development. |
|---|