Análisis bioinformático comparativo de la endolisina LysGH15 con otras endolisinas relacionadas
38 páginas : gráficas
- Autores:
-
Corredor Chavarro, Laura
Puerto Pérez, Cristian Leonardo
- Tipo de recurso:
- Trabajo de grado de pregrado
- Fecha de publicación:
- 2018
- Institución:
- Universidad de Ciencias Aplicadas y Ambientales U.D.C.A
- Repositorio:
- Repositorio Institucional UDCA
- Idioma:
- spa
- OAI Identifier:
- oai:repository.udca.edu.co:11158/3562
- Acceso en línea:
- https://repository.udca.edu.co/handle/11158/3562
- Palabra clave:
- Proteínas
Farmacorresistencia Microbiana
Endolisinas
LysGH15
Bioinformática
- Rights
- closedAccess
- License
- Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales
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Análisis bioinformático comparativo de la endolisina LysGH15 con otras endolisinas relacionadas |
| title |
Análisis bioinformático comparativo de la endolisina LysGH15 con otras endolisinas relacionadas |
| spellingShingle |
Análisis bioinformático comparativo de la endolisina LysGH15 con otras endolisinas relacionadas Proteínas Farmacorresistencia Microbiana Endolisinas LysGH15 Bioinformática |
| title_short |
Análisis bioinformático comparativo de la endolisina LysGH15 con otras endolisinas relacionadas |
| title_full |
Análisis bioinformático comparativo de la endolisina LysGH15 con otras endolisinas relacionadas |
| title_fullStr |
Análisis bioinformático comparativo de la endolisina LysGH15 con otras endolisinas relacionadas |
| title_full_unstemmed |
Análisis bioinformático comparativo de la endolisina LysGH15 con otras endolisinas relacionadas |
| title_sort |
Análisis bioinformático comparativo de la endolisina LysGH15 con otras endolisinas relacionadas |
| dc.creator.fl_str_mv |
Corredor Chavarro, Laura Puerto Pérez, Cristian Leonardo |
| dc.contributor.advisor.spa.fl_str_mv |
Fandiño Grisales, Julieta, dir. |
| dc.contributor.author.spa.fl_str_mv |
Corredor Chavarro, Laura Puerto Pérez, Cristian Leonardo |
| dc.subject.mesh.spa.fl_str_mv |
Proteínas Farmacorresistencia Microbiana |
| topic |
Proteínas Farmacorresistencia Microbiana Endolisinas LysGH15 Bioinformática |
| dc.subject.other.spa.fl_str_mv |
Endolisinas LysGH15 |
| dc.subject.lemb.spa.fl_str_mv |
Bioinformática |
| description |
38 páginas : gráficas |
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2018 |
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2018-08 |
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2020-08-31T15:28:39Z |
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2020-08-31T15:28:39Z |
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Trabajo de grado - Pregrado |
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Facultad de Ciencias |
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Tecnología de Regencia en Farmacia |
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Universidad de Ciencias Aplicadas y Ambientales U.D.C.A |
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Fandiño Grisales, Julieta, dir.Corredor Chavarro, LauraPuerto Pérez, Cristian Leonardo2020-08-31T15:28:39Z2020-08-31T15:28:39Z2018-08https://repository.udca.edu.co/handle/11158/356238 páginas : gráficasIn the present project a comparative bioinformatic study of the endolysin LysGH15 encoded by bacteriophage GH15 and related endolysins used as an alternative in the treatment of bacterial infections resistant to antibiotics was carried out. The study was performed using bioinformatics tools such as GenBank used to obtain the amino acid sequence, to establish the biochemical parameters of the LysGH15 endolysin, the tool ProtParam of the ExPASy was used and, in regard to the structures of the endolysins, they were downloaded from Protein Data Bank .For the superposition of the structures the bioinformatic tool Raptor X was used. While, the multiple sequence alignment was performed with MAFFT. From the in silico comparison it was obtained that LysGH15 shows similarities in its sequence and catalytic residues (CYS54, HIS117 and ASP56) with LysK. While with the other endolysins only share some regions in their sequence and present variations in their structureEn el presente proyecto se llevó a cabo un estudio bioinformático comparativo de la endolisina LysGH15 codificada por el bacteriófago GH15 y endolisinas relacionadas utilizadas como alternativa en el tratamiento de infecciones bacterianas resistentes a los antibióticos. El estudio se realizó usando herramientas bioinformáticas como GenBank empleado para obtener la secuencia aminoacídica, para establecer los parámetros bioquímicos de la endolisina LysGH15 se usó la herramienta ProtParam del ExPASy y en cuanto a la obtención de las estructuras de las endolisinas se descargaron de Protein Data Bank. Para superponer las estructuras se usó la herramienta Raptor X. Mientras, que la alineación de secuencias múltiples se realizó con MAFFT. De la comparación in silico se obtuvo que LysGH15 muestra similitudes en su secuencia y residuos catalíticos (CYS54, HIS117 y ASP56) con LysK. Mientras que con las otras endolisinas solo comparten algunas regiones en su secuencia y presenta variaciones en su estructuraIncluye bibliografíaPregradoTecnólogo(a) en Regencia de Farmaciaapplication/pdfspaBogotá : Corporación Tecnológica de Bogotá, 2018Facultad de CienciasTecnología de Regencia en FarmaciaDerechos Reservados - Universidad de Ciencias Aplicadas y Ambientaleshttps://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/closedAccessAtribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)http://purl.org/coar/access_right/c_14cbProteínasFarmacorresistencia MicrobianaEndolisinasLysGH15BioinformáticaAnálisis bioinformático comparativo de la endolisina LysGH15 con otras endolisinas relacionadasTrabajo de grado - Pregradohttp://purl.org/coar/resource_type/c_7a1finfo:eu-repo/semantics/bachelorThesisinfo:eu-repo/semantics/publishedVersionTexthttp://purl.org/redcol/resource_type/TPhttp://purl.org/coar/version/c_970fb48d4fbd8a85PublicationORIGINALTESIS FINAL.pdfTESIS FINAL.pdfapplication/pdf1216351https://repository.udca.edu.co/bitstreams/32b8613f-5c26-4f07-9699-99ad6bb9e187/download7b03b32710f5c8a6dc756cf0e826676bMD512. 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Articulo Cientifico .docx.txtExtracted texttext/plain24618https://repository.udca.edu.co/bitstreams/2054a149-7c0b-48a0-b3aa-11746c63df94/download95873a099db5c674743d60c3c39ea5d3MD56THUMBNAILTESIS FINAL.pdf.jpgTESIS FINAL.pdf.jpgGenerated Thumbnailimage/jpeg2752https://repository.udca.edu.co/bitstreams/fa68a1ac-038c-46b2-81d0-9d33a108e29e/downloadd570e4a4a516a4af83c318becda7896aMD5511158/3562oai:repository.udca.edu.co:11158/35622024-05-10 03:01:47.532https://creativecommons.org/licenses/by-nc-sa/4.0/Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientalesrestrictedhttps://repository.udca.edu.coRepositorio - Universidad de Ciencias Aplicadas y Ambientales 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en las Obras Colectivas;
b.	Distribuir copias o fonogramas de las Obras, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública, incluyéndolas como incorporadas en Obras Colectivas, según corresponda;
c.	Distribuir copias de las Obras Derivadas que se generen, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública.
Los derechos mencionados anteriormente pueden ser ejercidos en todos los medios y formatos, actualmente conocidos o que se inventen en el futuro. Los derechos antes mencionados incluyen el derecho a realizar dichas modificaciones en la medida que sean técnicamente necesarias para ejercer los derechos en otro medio o formatos, pero de otra manera usted no está autorizado para realizar obras derivadas.Todos los derechos no otorgados expresamente por el Licenciante quedan por este medio reservados, incluyendo pero sin limitarse a aquellos que se mencionan en las secciones 4(d) y 4(e).
4. Restricciones.
La licencia otorgada en la anterior Sección 3 está expresamente sujeta y limitada por las siguientes restricciones:
a.	Usted puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra sólo bajo las condiciones de esta Licencia, y Usted debe incluir una copia de esta licencia o del Identificador Universal de Recursos de la misma con cada copia de la Obra que distribuya, exhiba públicamente, ejecute públicamente o ponga a disposición pública. No es posible ofrecer o imponer ninguna condición sobre la Obra que altere o limite las condiciones de esta Licencia o el ejercicio de los derechos de los destinatarios otorgados en este documento. No es posible sublicenciar la Obra. Usted debe mantener intactos todos los avisos que hagan referencia a esta Licencia y a la cláusula de limitación de garantías. Usted no puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra con alguna medida tecnológica que controle el acceso o la utilización de ella de una forma que sea inconsistente con las condiciones de esta Licencia. Lo anterior se aplica a la Obra incorporada a una Obra Colectiva, pero esto no exige que la Obra Colectiva aparte de la obra misma quede sujeta a las condiciones de esta Licencia. Si Usted crea una Obra Colectiva, previo aviso de cualquier Licenciante debe, en la medida de lo posible, eliminar de la Obra Colectiva cualquier referencia a dicho Licenciante o al Autor Original, según lo solicitado por el Licenciante y conforme lo exige la cláusula 4(c).
b.	Usted no puede ejercer ninguno de los derechos que le han sido otorgados en la Sección 3 precedente de modo que estén principalmente destinados o directamente dirigidos a conseguir un provecho comercial o una compensación monetaria privada. El intercambio de la Obra por otras obras protegidas por derechos de autor, ya sea a través de un sistema para compartir archivos digitales (digital file-sharing) o de cualquier otra manera no será considerado como estar destinado principalmente o dirigido directamente a conseguir un provecho comercial o una compensación monetaria privada, siempre que no se realice un pago mediante una compensación monetaria en relación con el intercambio de obras protegidas por el derecho de autor.
c.	Si usted distribuye, exhibe públicamente, ejecuta públicamente o ejecuta públicamente en forma digital la Obra o cualquier Obra Derivada u Obra Colectiva, Usted debe mantener intacta toda la información de derecho de autor de la Obra y proporcionar, de forma razonable según el medio o manera que Usted esté utilizando: (i) el nombre del Autor Original si está provisto (o seudónimo, si fuere aplicable), y/o (ii) el nombre de la parte o las partes que el Autor Original y/o el Licenciante hubieren designado para la atribución (v.g., un instituto patrocinador, editorial, publicación) en la información de los derechos de autor del Licenciante, términos de servicios o de otras formas razonables; el título de la Obra si está provisto; en la medida de lo razonablemente factible y, si está provisto, el Identificador Uniforme de Recursos (Uniform Resource Identifier) que el Licenciante especifica para ser asociado con la Obra, salvo que tal URI no se refiera a la nota sobre los derechos de autor o a la información sobre el licenciamiento de la Obra; y en el caso de una Obra Derivada, atribuir el crédito identificando el uso de la Obra en la Obra Derivada (v.g., "Traducción Francesa de la Obra del Autor Original," o "Guión Cinematográfico basado en la Obra original del Autor Original"). Tal crédito puede ser implementado de cualquier forma razonable; en el caso, sin embargo, de Obras Derivadas u Obras Colectivas, tal crédito aparecerá, como mínimo, donde aparece el crédito de cualquier otro autor comparable y de una manera, al menos, tan destacada como el crédito de otro autor comparable.
d.	Para evitar toda confusión, el Licenciante aclara que, cuando la obra es una composición musical:
i.	Regalías por interpretación y ejecución bajo licencias generales. El Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública o la ejecución pública digital de la obra y de recolectar, sea individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, SAYCO), las regalías por la ejecución pública o por la ejecución pública digital de la obra (por ejemplo Webcast) licenciada bajo licencias generales, si la interpretación o ejecución de la obra está primordialmente orientada por o dirigida a la obtención de una ventaja comercial o una compensación monetaria privada.
ii.	Regalías por Fonogramas. El Licenciante se reserva el derecho exclusivo de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, SAYCO), una agencia de derechos musicales o algún agente designado, las regalías por cualquier fonograma que Usted cree a partir de la obra (“versión cover”) y distribuya, en los términos del régimen de derechos de autor, si la creación o distribución de esa versión cover está primordialmente destinada o dirigida a obtener una ventaja comercial o una compensación monetaria privada.
e.	Gestión de Derechos de Autor sobre Interpretaciones y Ejecuciones Digitales (WebCasting). Para evitar toda confusión, el Licenciante aclara que, cuando la obra sea un fonograma, el Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública digital de la obra (por ejemplo, webcast) y de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, Acinpro), las regalías por la ejecución pública digital de la obra (por ejemplo, webcast), sujeta a las disposiciones aplicables del régimen de Derecho de Autor, si esta ejecución pública digital está primordialmente dirigida a obtener una ventaja comercial o una compensación monetaria privada.
5. Representaciones, Garantías y Limitaciones de Responsabilidad.
A MENOS QUE LAS PARTES LO ACORDARAN DE OTRA FORMA POR ESCRITO, EL LICENCIANTE OFRECE LA OBRA (EN EL ESTADO EN EL QUE SE ENCUENTRA) “TAL CUAL”, SIN BRINDAR GARANTÍAS DE CLASE ALGUNA RESPECTO DE LA OBRA, YA SEA EXPRESA, IMPLÍCITA, LEGAL O CUALQUIERA OTRA, INCLUYENDO, SIN LIMITARSE A ELLAS, GARANTÍAS DE TITULARIDAD, COMERCIABILIDAD, ADAPTABILIDAD O ADECUACIÓN A PROPÓSITO DETERMINADO, AUSENCIA DE INFRACCIÓN, DE AUSENCIA DE DEFECTOS LATENTES O DE OTRO TIPO, O LA PRESENCIA O AUSENCIA DE ERRORES, SEAN O NO DESCUBRIBLES (PUEDAN O NO SER ESTOS DESCUBIERTOS). ALGUNAS JURISDICCIONES NO PERMITEN LA EXCLUSIÓN DE GARANTÍAS IMPLÍCITAS, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.
6. Limitación de responsabilidad.
A MENOS QUE LO EXIJA EXPRESAMENTE LA LEY APLICABLE, EL LICENCIANTE NO SERÁ RESPONSABLE ANTE USTED POR DAÑO ALGUNO, SEA POR RESPONSABILIDAD EXTRACONTRACTUAL, PRECONTRACTUAL O CONTRACTUAL, OBJETIVA O SUBJETIVA, SE TRATE DE DAÑOS MORALES O PATRIMONIALES, DIRECTOS O INDIRECTOS, PREVISTOS O IMPREVISTOS PRODUCIDOS POR EL USO DE ESTA LICENCIA O DE LA OBRA, AUN CUANDO EL LICENCIANTE HAYA SIDO ADVERTIDO DE LA POSIBILIDAD DE DICHOS DAÑOS. ALGUNAS LEYES NO PERMITEN LA EXCLUSIÓN DE CIERTA RESPONSABILIDAD, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.
7. Término.
a.	Esta Licencia y los derechos otorgados en virtud de ella terminarán automáticamente si Usted infringe alguna condición establecida en ella. Sin embargo, los individuos o entidades que han recibido Obras Derivadas o Colectivas de Usted de conformidad con esta Licencia, no verán terminadas sus licencias, siempre que estos individuos o entidades sigan cumpliendo íntegramente las condiciones de estas licencias. Las Secciones 1, 2, 5, 6, 7, y 8 subsistirán a cualquier terminación de esta Licencia.
b.	Sujeta a las condiciones y términos anteriores, la licencia otorgada aquí es perpetua (durante el período de vigencia de los derechos de autor de la obra). No obstante lo anterior, el Licenciante se reserva el derecho a publicar y/o estrenar la Obra bajo condiciones de licencia diferentes o a dejar de distribuirla en los términos de esta Licencia en cualquier momento; en el entendido, sin embargo, que esa elección no servirá para revocar esta licencia o que deba ser otorgada , bajo los términos de esta licencia), y esta licencia continuará en pleno vigor y efecto a menos que sea terminada como se expresa atrás. La Licencia revocada continuará siendo plenamente vigente y efectiva si no se le da término en las condiciones indicadas anteriormente.
8. Varios.
a.	Cada vez que Usted distribuya o ponga a disposición pública la Obra o una Obra Colectiva, el Licenciante ofrecerá al destinatario una licencia en los mismos términos y condiciones que la licencia otorgada a Usted bajo esta Licencia.
b.	Si alguna disposición de esta Licencia resulta invalidada o no exigible, según la legislación vigente, esto no afectará ni la validez ni la aplicabilidad del resto de condiciones de esta Licencia y, sin acción adicional por parte de los sujetos de este acuerdo, aquélla se entenderá reformada lo mínimo necesario para hacer que dicha disposición sea válida y exigible.
c.	Ningún término o disposición de esta Licencia se estimará renunciada y ninguna violación de ella será consentida a menos que esa renuncia o consentimiento sea otorgado por escrito y firmado por la parte que renuncie o consienta.
d.	Esta Licencia refleja el acuerdo pleno entre las partes respecto a la Obra aquí licenciada. No hay arreglos, acuerdos o declaraciones respecto a la Obra que no estén especificados en este documento. El Licenciante no se verá limitado por ninguna disposición adicional que pueda surgir en alguna comunicación emanada de Usted. Esta Licencia no puede ser modificada sin el consentimiento mutuo por escrito del Licenciante y Usted.

 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