Evaluation of the effectiveness of platelet-rich plasma in surgical epidermal wounds in rabbits
Platelet-rich plasma (PRP) is the portion of the blood plasma which contains a platelet concentration above basal levels that stimulate tissue repair by means to growth factors. The aimed of this research was to evaluate the impact of PRP on skin repair upon epidermal surgical lesions. Using a scalp...
- Autores:
-
Bonilla Gutiérrez, Andrés Felipe
Rondón Barragán, Iang Schroniltgen
Barbosa Sánchez, Irma Ximena
Aristizábal Páez, Omar Leonardo
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2018
- Institución:
- Universidad de Ciencias Aplicadas y Ambientales U.D.C.A
- Repositorio:
- Repositorio Institucional UDCA
- Idioma:
- eng
- OAI Identifier:
- oai:repository.udca.edu.co:11158/1450
- Acceso en línea:
- https://revistas.udca.edu.co/index.php/ruadc/article/view/673
- Palabra clave:
- Medicina Regenerativa
Platelet-rich plasma
Animal models
Regenerative medicine
Plasma sanguíneo
Conejo (oryctolagus)
- Rights
- openAccess
- License
- Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales, 2018
| Summary: | Platelet-rich plasma (PRP) is the portion of the blood plasma which contains a platelet concentration above basal levels that stimulate tissue repair by means to growth factors. The aimed of this research was to evaluate the impact of PRP on skin repair upon epidermal surgical lesions. Using a scalpel, a clean epidermal incision of 5cm length was made in the interscapular region of New Zealand rabbits (n=8), and then divided in two groups. Rabbits from the group 1 (n=4) were untreated (control group), while group 2 received PRP on the surgical wound. Autologous PRP was obtained from whole blood drawn by jugular vein puncture, collected with sodium citrate anticoagulant solution, and centrifuged at 120g x 5min. One milliliter was obtained of the phase superior to the buffy coat. Biopsies from the injured tissue were collected on days 3, 7, 14 and 21 post-surgeries, and submitted to Hematoxylin-Eosin and Masson’s Trichrome staining. In conclusion, the platelets separation method allows to obtaining plasma with higher platelets concentration and reduced number of leukocytes. Additionally, the results show that therapeutic PRP administration on surgically-induced skin injury has effect on the histological features of tissue healing compared with the control group. |
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