Estudio histológico y morfológico del desarrollo embrionario del pez capitán de la sabana (Eremophilus mutisii)
Eremophilus mutisii, due to its vulnerable condition and its potential for fish farming, generates research challenges; one of them is to know the embryonic changes of the E. mutisiibased on morphological and histological features from fer-tilization to hatching. The embryos were obtained from adult...
- Autores:
-
Moncaleano Gómez, Erika
Sánchez, Carlos
Prieto Mojica, Camilo
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2018
- Institución:
- Universidad de Ciencias Aplicadas y Ambientales U.D.C.A
- Repositorio:
- Repositorio Institucional UDCA
- Idioma:
- spa
- OAI Identifier:
- oai:repository.udca.edu.co:11158/1524
- Acceso en línea:
- https://revistas.udca.edu.co/index.php/ruadc/article/view/1073
https://doi.org/10.31910/rudca.v21.n2.2018.1073
- Palabra clave:
- Capitán de la sabana
Desarrollo embrionario
Capitán de la sabana
Embriogénesis
Siluriformes
Trichomycteridae
Desarrollo embrionario
Siluriformes
Trichomycteridae
- Rights
- openAccess
- License
- Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales, 2018
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Estudio histológico y morfológico del desarrollo embrionario del pez capitán de la sabana (Eremophilus mutisii) |
| dc.title.alternative.eng.fl_str_mv |
Histological and morphological study of the embrionary development of the capitan de la sabana (Eremophilus mutisii) |
| title |
Estudio histológico y morfológico del desarrollo embrionario del pez capitán de la sabana (Eremophilus mutisii) |
| spellingShingle |
Estudio histológico y morfológico del desarrollo embrionario del pez capitán de la sabana (Eremophilus mutisii) Capitán de la sabana Desarrollo embrionario Capitán de la sabana Embriogénesis Siluriformes Trichomycteridae Desarrollo embrionario Siluriformes Trichomycteridae |
| title_short |
Estudio histológico y morfológico del desarrollo embrionario del pez capitán de la sabana (Eremophilus mutisii) |
| title_full |
Estudio histológico y morfológico del desarrollo embrionario del pez capitán de la sabana (Eremophilus mutisii) |
| title_fullStr |
Estudio histológico y morfológico del desarrollo embrionario del pez capitán de la sabana (Eremophilus mutisii) |
| title_full_unstemmed |
Estudio histológico y morfológico del desarrollo embrionario del pez capitán de la sabana (Eremophilus mutisii) |
| title_sort |
Estudio histológico y morfológico del desarrollo embrionario del pez capitán de la sabana (Eremophilus mutisii) |
| dc.creator.fl_str_mv |
Moncaleano Gómez, Erika Sánchez, Carlos Prieto Mojica, Camilo |
| dc.contributor.author.spa.fl_str_mv |
Moncaleano Gómez, Erika Sánchez, Carlos Prieto Mojica, Camilo |
| dc.subject.lemb.spa.fl_str_mv |
Capitán de la sabana |
| topic |
Capitán de la sabana Desarrollo embrionario Capitán de la sabana Embriogénesis Siluriformes Trichomycteridae Desarrollo embrionario Siluriformes Trichomycteridae |
| dc.subject.proposal.spa.fl_str_mv |
Desarrollo embrionario Capitán de la sabana Embriogénesis Siluriformes Trichomycteridae |
| dc.subject.agrovoc.spa.fl_str_mv |
Desarrollo embrionario Siluriformes Trichomycteridae |
| description |
Eremophilus mutisii, due to its vulnerable condition and its potential for fish farming, generates research challenges; one of them is to know the embryonic changes of the E. mutisiibased on morphological and histological features from fer-tilization to hatching. The embryos were obtained from adult and sexually mature individuals of the Capitán de la Sabana (Eremophilus mutisii), sexually induced with carp pituitary extract. Oocytes and semen were obtained by abdominal pressure 24 hours after the last dose at 14±1°C. Sampling was performed every 60 minutes for the first twelve hours ́ post-fertilization (HPF); from here on at hatching, the sam-ples were fixed every 6 hours. The embryos were fixed in 4% formalin for observing the morphological features and for the histological studies. Results revealed that the fertilized eggs had spherical shape, light green color and average diameter of 1270μm. The first cleavage at 2 HPF was observed, the blastulation began at 9 HPF. Epibolic movements and gastru-lation were observed from 12 HPF to 30 HPF with blastopore closure. At 60 HPF the yolk sac tail detachment was evident and at 66 HPF the first autonomous movements began. Finally, at 72 HPF, the hatching began, showing absence of pigmentation and evidencing a rudimentary digestive tract without oral and anal opening. It was observed the presence of abundant yolk sac with continuous vertical movements of the larva. |
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2018 |
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2018-07 |
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2019-06-10T16:39:55Z |
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2019-06-10T16:39:55Z |
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Artículo de revista |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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http://purl.org/coar/resource_type/c_6501 |
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info:eu-repo/semantics/article |
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Text |
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http://purl.org/redcol/resource_type/ART |
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0123-4226 |
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https://revistas.udca.edu.co/index.php/ruadc/article/view/1073 |
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https://doi.org/10.31910/rudca.v21.n2.2018.1073 |
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307380 |
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https://revistas.udca.edu.co/index.php/ruadc/article/view/1073 https://doi.org/10.31910/rudca.v21.n2.2018.1073 |
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spa |
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spa |
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Revista UDCA : Actualidad & Divulgación Científica (Bogotá). -- Vol. 21, No. 2 (Jul.-Dic. 2018). -- páginas 479-490 |
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Agricultura |
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Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales, 2018 |
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Bogotá : Universidad de Ciencias Aplicadas y Ambientales, 2018 |
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Moncaleano Gómez, ErikaSánchez, CarlosPrieto Mojica, Camilo2019-06-10T16:39:55Z2019-06-10T16:39:55Z2018-070123-4226https://revistas.udca.edu.co/index.php/ruadc/article/view/1073https://doi.org/10.31910/rudca.v21.n2.2018.1073307380Eremophilus mutisii, due to its vulnerable condition and its potential for fish farming, generates research challenges; one of them is to know the embryonic changes of the E. mutisiibased on morphological and histological features from fer-tilization to hatching. The embryos were obtained from adult and sexually mature individuals of the Capitán de la Sabana (Eremophilus mutisii), sexually induced with carp pituitary extract. Oocytes and semen were obtained by abdominal pressure 24 hours after the last dose at 14±1°C. Sampling was performed every 60 minutes for the first twelve hours ́ post-fertilization (HPF); from here on at hatching, the sam-ples were fixed every 6 hours. The embryos were fixed in 4% formalin for observing the morphological features and for the histological studies. Results revealed that the fertilized eggs had spherical shape, light green color and average diameter of 1270μm. The first cleavage at 2 HPF was observed, the blastulation began at 9 HPF. Epibolic movements and gastru-lation were observed from 12 HPF to 30 HPF with blastopore closure. At 60 HPF the yolk sac tail detachment was evident and at 66 HPF the first autonomous movements began. Finally, at 72 HPF, the hatching began, showing absence of pigmentation and evidencing a rudimentary digestive tract without oral and anal opening. It was observed the presence of abundant yolk sac with continuous vertical movements of the larva.Eremophilus mutisii, pez catalogado en estado como vulnerable, que por su importancia sociocultural y ecológica genera retos investigativos, uno de ellos, conocer sus características histológicas del desarrollo embrionario, desde la fecundación hasta la eclosión. Para ello, se obtuvieron embriones de individuos adultos y maduros sexualmente, capturados en la represa del Sisga, ubicada en el municipio de Chocontá, Cundinamarca, inducidos sexualmente con extracto pituitario de carpa. Los ovocitos y el semen fueron obtenidos por presión abdominal 24 horas después de la última dosis, a 14±1°C. La toma de muestras, se realizó cada 60 minutos, durante las primeras doce horas post-fertilización (HPF); a partir de aquí y hasta la eclosión, las muestras fueron fijadas en formol Bufferado al 4%, cada 6 horas y sometidas a rutina de histología. Los resultados mostraron que los óvulos fertilizados presentaron forma esférica, color verde claro y diámetro promedio de 1270µm. El primer clivaje, se observó a las 2HPF; la blastulación inició a las 9HPF; los movimientos epibólicos y la gastrulación, se observaron desde la 12HPF hasta la 30HPF, con el cierre del blastoporo. A las 60HPF, se evidenció el desprendimiento de la cola del saco vitelino y a las 66HPF, se dio inicio a los primeros movimientos autónomos. Finalmente, a las 72HPF inició la eclosión, mostrando las larvas ausencia de pigmentación y evidenciándose un tracto digestivo rudimentario, sin abertura bucal y anal. Se observó la presencia de abundante saco vitelino, con movimientos verticales continuos de la larva.Incluye referencias bibliográficasapplication/pdfspaBogotá : Universidad de Ciencias Aplicadas y Ambientales, 2018Revista UDCA : Actualidad & Divulgación Científica (Bogotá). -- Vol. 21, No. 2 (Jul.-Dic. 2018). -- páginas 479-490AgriculturaDerechos Reservados - Universidad de Ciencias Aplicadas y Ambientales, 2018https://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccessAtribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)http://purl.org/coar/access_right/c_abf2Estudio histológico y morfológico del desarrollo embrionario del pez capitán de la sabana (Eremophilus mutisii)Histological and morphological study of the embrionary development of the capitan de la sabana (Eremophilus mutisii)Artículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionTexthttp://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85Capitán de la sabanaDesarrollo embrionarioCapitán de la sabanaEmbriogénesisSiluriformesTrichomycteridaeDesarrollo embrionarioSiluriformesTrichomycteridaePublicationORIGINAL1073-Texto del artículo-5405-1-10-20181215.pdf1073-Texto del artículo-5405-1-10-20181215.pdfapplication/pdf4542256https://repository.udca.edu.co/bitstreams/0ebb4c6e-b014-4b61-a5d6-0112e191237e/download5eb5a1a5b4a9ac011cef5a91d0cf978cMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-814775https://repository.udca.edu.co/bitstreams/433fbed7-0ee7-474c-ab73-7d75610ac3a3/downloadf661acf14bedbf9f5d13897a0387e751MD52TEXT1073-Texto del artículo-5405-1-10-20181215.pdf.txt1073-Texto del artículo-5405-1-10-20181215.pdf.txtExtracted 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en las Obras Colectivas;
b.	Distribuir copias o fonogramas de las Obras, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública, incluyéndolas como incorporadas en Obras Colectivas, según corresponda;
c.	Distribuir copias de las Obras Derivadas que se generen, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública.
Los derechos mencionados anteriormente pueden ser ejercidos en todos los medios y formatos, actualmente conocidos o que se inventen en el futuro. Los derechos antes mencionados incluyen el derecho a realizar dichas modificaciones en la medida que sean técnicamente necesarias para ejercer los derechos en otro medio o formatos, pero de otra manera usted no está autorizado para realizar obras derivadas.Todos los derechos no otorgados expresamente por el Licenciante quedan por este medio reservados, incluyendo pero sin limitarse a aquellos que se mencionan en las secciones 4(d) y 4(e).
4. Restricciones.
La licencia otorgada en la anterior Sección 3 está expresamente sujeta y limitada por las siguientes restricciones:
a.	Usted puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra sólo bajo las condiciones de esta Licencia, y Usted debe incluir una copia de esta licencia o del Identificador Universal de Recursos de la misma con cada copia de la Obra que distribuya, exhiba públicamente, ejecute públicamente o ponga a disposición pública. No es posible ofrecer o imponer ninguna condición sobre la Obra que altere o limite las condiciones de esta Licencia o el ejercicio de los derechos de los destinatarios otorgados en este documento. No es posible sublicenciar la Obra. Usted debe mantener intactos todos los avisos que hagan referencia a esta Licencia y a la cláusula de limitación de garantías. Usted no puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra con alguna medida tecnológica que controle el acceso o la utilización de ella de una forma que sea inconsistente con las condiciones de esta Licencia. Lo anterior se aplica a la Obra incorporada a una Obra Colectiva, pero esto no exige que la Obra Colectiva aparte de la obra misma quede sujeta a las condiciones de esta Licencia. Si Usted crea una Obra Colectiva, previo aviso de cualquier Licenciante debe, en la medida de lo posible, eliminar de la Obra Colectiva cualquier referencia a dicho Licenciante o al Autor Original, según lo solicitado por el Licenciante y conforme lo exige la cláusula 4(c).
b.	Usted no puede ejercer ninguno de los derechos que le han sido otorgados en la Sección 3 precedente de modo que estén principalmente destinados o directamente dirigidos a conseguir un provecho comercial o una compensación monetaria privada. El intercambio de la Obra por otras obras protegidas por derechos de autor, ya sea a través de un sistema para compartir archivos digitales (digital file-sharing) o de cualquier otra manera no será considerado como estar destinado principalmente o dirigido directamente a conseguir un provecho comercial o una compensación monetaria privada, siempre que no se realice un pago mediante una compensación monetaria en relación con el intercambio de obras protegidas por el derecho de autor.
c.	Si usted distribuye, exhibe públicamente, ejecuta públicamente o ejecuta públicamente en forma digital la Obra o cualquier Obra Derivada u Obra Colectiva, Usted debe mantener intacta toda la información de derecho de autor de la Obra y proporcionar, de forma razonable según el medio o manera que Usted esté utilizando: (i) el nombre del Autor Original si está provisto (o seudónimo, si fuere aplicable), y/o (ii) el nombre de la parte o las partes que el Autor Original y/o el Licenciante hubieren designado para la atribución (v.g., un instituto patrocinador, editorial, publicación) en la información de los derechos de autor del Licenciante, términos de servicios o de otras formas razonables; el título de la Obra si está provisto; en la medida de lo razonablemente factible y, si está provisto, el Identificador Uniforme de Recursos (Uniform Resource Identifier) que el Licenciante especifica para ser asociado con la Obra, salvo que tal URI no se refiera a la nota sobre los derechos de autor o a la información sobre el licenciamiento de la Obra; y en el caso de una Obra Derivada, atribuir el crédito identificando el uso de la Obra en la Obra Derivada (v.g., "Traducción Francesa de la Obra del Autor Original," o "Guión Cinematográfico basado en la Obra original del Autor Original"). Tal crédito puede ser implementado de cualquier forma razonable; en el caso, sin embargo, de Obras Derivadas u Obras Colectivas, tal crédito aparecerá, como mínimo, donde aparece el crédito de cualquier otro autor comparable y de una manera, al menos, tan destacada como el crédito de otro autor comparable.
d.	Para evitar toda confusión, el Licenciante aclara que, cuando la obra es una composición musical:
i.	Regalías por interpretación y ejecución bajo licencias generales. El Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública o la ejecución pública digital de la obra y de recolectar, sea individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, SAYCO), las regalías por la ejecución pública o por la ejecución pública digital de la obra (por ejemplo Webcast) licenciada bajo licencias generales, si la interpretación o ejecución de la obra está primordialmente orientada por o dirigida a la obtención de una ventaja comercial o una compensación monetaria privada.
ii.	Regalías por Fonogramas. El Licenciante se reserva el derecho exclusivo de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, SAYCO), una agencia de derechos musicales o algún agente designado, las regalías por cualquier fonograma que Usted cree a partir de la obra (“versión cover”) y distribuya, en los términos del régimen de derechos de autor, si la creación o distribución de esa versión cover está primordialmente destinada o dirigida a obtener una ventaja comercial o una compensación monetaria privada.
e.	Gestión de Derechos de Autor sobre Interpretaciones y Ejecuciones Digitales (WebCasting). Para evitar toda confusión, el Licenciante aclara que, cuando la obra sea un fonograma, el Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública digital de la obra (por ejemplo, webcast) y de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, Acinpro), las regalías por la ejecución pública digital de la obra (por ejemplo, webcast), sujeta a las disposiciones aplicables del régimen de Derecho de Autor, si esta ejecución pública digital está primordialmente dirigida a obtener una ventaja comercial o una compensación monetaria privada.
5. Representaciones, Garantías y Limitaciones de Responsabilidad.
A MENOS QUE LAS PARTES LO ACORDARAN DE OTRA FORMA POR ESCRITO, EL LICENCIANTE OFRECE LA OBRA (EN EL ESTADO EN EL QUE SE ENCUENTRA) “TAL CUAL”, SIN BRINDAR GARANTÍAS DE CLASE ALGUNA RESPECTO DE LA OBRA, YA SEA EXPRESA, IMPLÍCITA, LEGAL O CUALQUIERA OTRA, INCLUYENDO, SIN LIMITARSE A ELLAS, GARANTÍAS DE TITULARIDAD, COMERCIABILIDAD, ADAPTABILIDAD O ADECUACIÓN A PROPÓSITO DETERMINADO, AUSENCIA DE INFRACCIÓN, DE AUSENCIA DE DEFECTOS LATENTES O DE OTRO TIPO, O LA PRESENCIA O AUSENCIA DE ERRORES, SEAN O NO DESCUBRIBLES (PUEDAN O NO SER ESTOS DESCUBIERTOS). ALGUNAS JURISDICCIONES NO PERMITEN LA EXCLUSIÓN DE GARANTÍAS IMPLÍCITAS, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.
6. Limitación de responsabilidad.
A MENOS QUE LO EXIJA EXPRESAMENTE LA LEY APLICABLE, EL LICENCIANTE NO SERÁ RESPONSABLE ANTE USTED POR DAÑO ALGUNO, SEA POR RESPONSABILIDAD EXTRACONTRACTUAL, PRECONTRACTUAL O CONTRACTUAL, OBJETIVA O SUBJETIVA, SE TRATE DE DAÑOS MORALES O PATRIMONIALES, DIRECTOS O INDIRECTOS, PREVISTOS O IMPREVISTOS PRODUCIDOS POR EL USO DE ESTA LICENCIA O DE LA OBRA, AUN CUANDO EL LICENCIANTE HAYA SIDO ADVERTIDO DE LA POSIBILIDAD DE DICHOS DAÑOS. ALGUNAS LEYES NO PERMITEN LA EXCLUSIÓN DE CIERTA RESPONSABILIDAD, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.
7. Término.
a.	Esta Licencia y los derechos otorgados en virtud de ella terminarán automáticamente si Usted infringe alguna condición establecida en ella. Sin embargo, los individuos o entidades que han recibido Obras Derivadas o Colectivas de Usted de conformidad con esta Licencia, no verán terminadas sus licencias, siempre que estos individuos o entidades sigan cumpliendo íntegramente las condiciones de estas licencias. Las Secciones 1, 2, 5, 6, 7, y 8 subsistirán a cualquier terminación de esta Licencia.
b.	Sujeta a las condiciones y términos anteriores, la licencia otorgada aquí es perpetua (durante el período de vigencia de los derechos de autor de la obra). No obstante lo anterior, el Licenciante se reserva el derecho a publicar y/o estrenar la Obra bajo condiciones de licencia diferentes o a dejar de distribuirla en los términos de esta Licencia en cualquier momento; en el entendido, sin embargo, que esa elección no servirá para revocar esta licencia o que deba ser otorgada , bajo los términos de esta licencia), y esta licencia continuará en pleno vigor y efecto a menos que sea terminada como se expresa atrás. La Licencia revocada continuará siendo plenamente vigente y efectiva si no se le da término en las condiciones indicadas anteriormente.
8. Varios.
a.	Cada vez que Usted distribuya o ponga a disposición pública la Obra o una Obra Colectiva, el Licenciante ofrecerá al destinatario una licencia en los mismos términos y condiciones que la licencia otorgada a Usted bajo esta Licencia.
b.	Si alguna disposición de esta Licencia resulta invalidada o no exigible, según la legislación vigente, esto no afectará ni la validez ni la aplicabilidad del resto de condiciones de esta Licencia y, sin acción adicional por parte de los sujetos de este acuerdo, aquélla se entenderá reformada lo mínimo necesario para hacer que dicha disposición sea válida y exigible.
c.	Ningún término o disposición de esta Licencia se estimará renunciada y ninguna violación de ella será consentida a menos que esa renuncia o consentimiento sea otorgado por escrito y firmado por la parte que renuncie o consienta.
d.	Esta Licencia refleja el acuerdo pleno entre las partes respecto a la Obra aquí licenciada. No hay arreglos, acuerdos o declaraciones respecto a la Obra que no estén especificados en este documento. El Licenciante no se verá limitado por ninguna disposición adicional que pueda surgir en alguna comunicación emanada de Usted. Esta Licencia no puede ser modificada sin el consentimiento mutuo por escrito del Licenciante y Usted.

 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