Halophilic bacteria with potential for the recovery of salinated soils in Sáchica-Boyacá, Colombia
Halophilic bacteria with potential for the recovery of salinated soils in Sáchica-Boyacá, Colombia. It is possible to use halophilic bacteria isolated from saline environments as a possible alternative for the rehabilitation ofsalinated soils for agricultural and agricultural production. Six halophi...
- Autores:
-
Rodríguez Aristizábal, Mónica Alejandra
Higuera Mora, Nubia Carolina
Sanjuanelo Corredor, Danny Wilson
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2019
- Institución:
- Universidad de Ciencias Aplicadas y Ambientales U.D.C.A
- Repositorio:
- Repositorio Institucional UDCA
- Idioma:
- eng
- OAI Identifier:
- oai:repository.udca.edu.co:11158/2081
- Palabra clave:
- Bioassay
Electrical conductivity
Halophilic bacteria
Saline soils
Salinization
Conductividad eléctrica
Salinización del suelo
Bacteria
Invernaderos
Plantas
Salinización del suelo
- Rights
- openAccess
- License
- Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales
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dc.title.spa.fl_str_mv |
Halophilic bacteria with potential for the recovery of salinated soils in Sáchica-Boyacá, Colombia |
dc.title.alternative.spa.fl_str_mv |
Bacterias halófilas con potencial para la recuperación de suelos salinizados en Sáchica-Boyacá, Colombia |
title |
Halophilic bacteria with potential for the recovery of salinated soils in Sáchica-Boyacá, Colombia |
spellingShingle |
Halophilic bacteria with potential for the recovery of salinated soils in Sáchica-Boyacá, Colombia Bioassay Electrical conductivity Halophilic bacteria Saline soils Salinization Conductividad eléctrica Salinización del suelo Bacteria Invernaderos Plantas Salinización del suelo |
title_short |
Halophilic bacteria with potential for the recovery of salinated soils in Sáchica-Boyacá, Colombia |
title_full |
Halophilic bacteria with potential for the recovery of salinated soils in Sáchica-Boyacá, Colombia |
title_fullStr |
Halophilic bacteria with potential for the recovery of salinated soils in Sáchica-Boyacá, Colombia |
title_full_unstemmed |
Halophilic bacteria with potential for the recovery of salinated soils in Sáchica-Boyacá, Colombia |
title_sort |
Halophilic bacteria with potential for the recovery of salinated soils in Sáchica-Boyacá, Colombia |
dc.creator.fl_str_mv |
Rodríguez Aristizábal, Mónica Alejandra Higuera Mora, Nubia Carolina Sanjuanelo Corredor, Danny Wilson |
dc.contributor.author.spa.fl_str_mv |
Rodríguez Aristizábal, Mónica Alejandra Higuera Mora, Nubia Carolina Sanjuanelo Corredor, Danny Wilson |
dc.subject.proposal.spa.fl_str_mv |
Bioassay Electrical conductivity Halophilic bacteria Saline soils Salinization |
topic |
Bioassay Electrical conductivity Halophilic bacteria Saline soils Salinization Conductividad eléctrica Salinización del suelo Bacteria Invernaderos Plantas Salinización del suelo |
dc.subject.agrovoc.spa.fl_str_mv |
Conductividad eléctrica Salinización del suelo Bacteria Invernaderos Plantas Salinización del suelo |
description |
Halophilic bacteria with potential for the recovery of salinated soils in Sáchica-Boyacá, Colombia. It is possible to use halophilic bacteria isolated from saline environments as a possible alternative for the rehabilitation ofsalinated soils for agricultural and agricultural production. Six halophilic bacteria were evaluated to determine their potential in the reduction of the electrical conductivity (Ce) of moderately saline soil in the municipality of Sáchica-Boyacá (Colombia). The bacteria were isolated on nutritive agar with different concentrations of NaCl, then inoculated in the soil (3 kg placed in pots) to perform a bioassay in the greenhouse for 2 months. Two phases were carried out, the first until obtaining a value of 2 ds/m (Ce) in order to plant tomato plants in the second phase. Weekly measurements of the (Ce) were made throughout the bioassay and only in phase 2 were determined weekly qualitative variables of tomato growth (height, number of leaves, foliar area, wilting and chlorosis). The results showed a direct effect of the halophilic bacteria in the decrease of the (Ce) of the soil, going from 5.2 to 0.8 ds/m (T1), 0.7 ds/m (T2), 0.8 ds/m (T3), 0.6 ds/m (T4), 0.7 ds/m (T5), 0.7 ds/m (T6), 3.0 ds/m (Control), values that correspond to very light salinity (< 2 ds/m). Regarding the agronomic variables, improvements were observed in the characteristics evaluated in comparison with the absolute control, especially in height and number of leaves of the plants. For the absolute control 80 % of the experimental units were lost, while in the soil with the different treatments only in some cases 20 % was lost. Accordingly, it is evident that the halophilic bacteria used in this research have potential for the rehabilitation of salinated soils. |
publishDate |
2019 |
dc.date.accessioned.spa.fl_str_mv |
2019-09-02T20:01:07Z |
dc.date.available.spa.fl_str_mv |
2019-09-02T20:01:07Z |
dc.date.issued.spa.fl_str_mv |
2019 |
dc.type.spa.fl_str_mv |
Artículo de revista |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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http://purl.org/coar/resource_type/c_6501 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/redcol/resource_type/ART |
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http://purl.org/coar/resource_type/c_6501 |
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dc.identifier.citation.spa.fl_str_mv |
Rodríguez Aristizabal, M.A., Higuera Mora, N.C., Sanjuanelo Corredor, D.W. Halophilic bacteria with potential for the recovery of salinated soils in Sáchica-Boyacá, Colombia [Article@Bacterias halófilas con potencial para la recuperación de suelos salinizados en Sáchica-Boyacá, Colombia] (2019) Revista de Biologia Tropical, 67 (3), pp. 621-632. |
dc.identifier.uri.spa.fl_str_mv |
https://www.researchgate.net/publication/333613738_Bacterias_halofilas_con_potencial_para_la_recuperacion_de_suelos_salinizados_en_Sachica-Boyaca_Colombia |
identifier_str_mv |
Rodríguez Aristizabal, M.A., Higuera Mora, N.C., Sanjuanelo Corredor, D.W. Halophilic bacteria with potential for the recovery of salinated soils in Sáchica-Boyacá, Colombia [Article@Bacterias halófilas con potencial para la recuperación de suelos salinizados en Sáchica-Boyacá, Colombia] (2019) Revista de Biologia Tropical, 67 (3), pp. 621-632. |
url |
https://www.researchgate.net/publication/333613738_Bacterias_halofilas_con_potencial_para_la_recuperacion_de_suelos_salinizados_en_Sachica-Boyaca_Colombia |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.spa.fl_str_mv |
Revista de Biologia Tropical;Vol.67, No.3, Jun 2019 páginas 621-632 |
dc.relation.indexed.spa.fl_str_mv |
Agricultura |
dc.rights.spa.fl_str_mv |
Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales |
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http://purl.org/coar/access_right/c_abf2 |
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Atribución-CompartirIgual 4.0 Internacional (CC BY-SA 4.0) |
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Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales https://creativecommons.org/licenses/by-sa/4.0/ Atribución-CompartirIgual 4.0 Internacional (CC BY-SA 4.0) http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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application/pdf |
dc.coverage.spatial.spa.fl_str_mv |
Colombia |
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Rodríguez Aristizábal, Mónica AlejandraHiguera Mora, Nubia CarolinaSanjuanelo Corredor, Danny WilsonColombia2019-09-02T20:01:07Z2019-09-02T20:01:07Z2019Rodríguez Aristizabal, M.A., Higuera Mora, N.C., Sanjuanelo Corredor, D.W. Halophilic bacteria with potential for the recovery of salinated soils in Sáchica-Boyacá, Colombia [Article@Bacterias halófilas con potencial para la recuperación de suelos salinizados en Sáchica-Boyacá, Colombia] (2019) Revista de Biologia Tropical, 67 (3), pp. 621-632.https://www.researchgate.net/publication/333613738_Bacterias_halofilas_con_potencial_para_la_recuperacion_de_suelos_salinizados_en_Sachica-Boyaca_ColombiaHalophilic bacteria with potential for the recovery of salinated soils in Sáchica-Boyacá, Colombia. It is possible to use halophilic bacteria isolated from saline environments as a possible alternative for the rehabilitation ofsalinated soils for agricultural and agricultural production. Six halophilic bacteria were evaluated to determine their potential in the reduction of the electrical conductivity (Ce) of moderately saline soil in the municipality of Sáchica-Boyacá (Colombia). The bacteria were isolated on nutritive agar with different concentrations of NaCl, then inoculated in the soil (3 kg placed in pots) to perform a bioassay in the greenhouse for 2 months. Two phases were carried out, the first until obtaining a value of 2 ds/m (Ce) in order to plant tomato plants in the second phase. Weekly measurements of the (Ce) were made throughout the bioassay and only in phase 2 were determined weekly qualitative variables of tomato growth (height, number of leaves, foliar area, wilting and chlorosis). The results showed a direct effect of the halophilic bacteria in the decrease of the (Ce) of the soil, going from 5.2 to 0.8 ds/m (T1), 0.7 ds/m (T2), 0.8 ds/m (T3), 0.6 ds/m (T4), 0.7 ds/m (T5), 0.7 ds/m (T6), 3.0 ds/m (Control), values that correspond to very light salinity (< 2 ds/m). Regarding the agronomic variables, improvements were observed in the characteristics evaluated in comparison with the absolute control, especially in height and number of leaves of the plants. For the absolute control 80 % of the experimental units were lost, while in the soil with the different treatments only in some cases 20 % was lost. Accordingly, it is evident that the halophilic bacteria used in this research have potential for the rehabilitation of salinated soils.application/pdfengRevista de Biologia Tropical;Vol.67, No.3, Jun 2019 páginas 621-632AgriculturaDerechos Reservados - Universidad de Ciencias Aplicadas y Ambientaleshttps://creativecommons.org/licenses/by-sa/4.0/info:eu-repo/semantics/openAccessAtribución-CompartirIgual 4.0 Internacional (CC BY-SA 4.0)http://purl.org/coar/access_right/c_abf2https://www.scopus.com/search/form.uri?display=basichttps://www.scopus.com/search/form.uri?display=basicHalophilic bacteria with potential for the recovery of salinated soils in Sáchica-Boyacá, ColombiaBacterias halófilas con potencial para la recuperación de suelos salinizados en Sáchica-Boyacá, ColombiaArtículo de 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 nombre de la parte o las partes que el Autor Original y/o el Licenciante hubieren designado para la atribución (v.g., un instituto patrocinador, editorial, publicación) en la información de los derechos de autor del Licenciante, términos de servicios o de otras formas razonables; el título de la Obra si está provisto; en la medida de lo razonablemente factible y, si está provisto, el Identificador Uniforme de Recursos (Uniform Resource Identifier) que el Licenciante especifica para ser asociado con la Obra, salvo que tal URI no se refiera a la nota sobre los derechos de autor o a la información sobre el licenciamiento de la Obra; y en el caso de una Obra Derivada, atribuir el crédito identificando el uso de la Obra en la Obra Derivada (v.g., "Traducción Francesa de la Obra del Autor Original," o "Guión Cinematográfico basado en la Obra original del Autor Original"). Tal crédito puede ser implementado de cualquier forma razonable; en el caso, sin embargo, de Obras Derivadas u Obras Colectivas, tal crédito aparecerá, como mínimo, donde aparece el crédito de cualquier otro autor comparable y de una manera, al menos, tan destacada como el crédito de otro autor comparable.
d.	Para evitar toda confusión, el Licenciante aclara que, cuando la obra es una composición musical:
i.	Regalías por interpretación y ejecución bajo licencias generales. El Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública o la ejecución pública digital de la obra y de recolectar, sea individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, SAYCO), las regalías por la ejecución pública o por la ejecución pública digital de la obra (por ejemplo Webcast) licenciada bajo licencias generales, si la interpretación o ejecución de la obra está primordialmente orientada por o dirigida a la obtención de una ventaja comercial o una compensación monetaria privada.
ii.	Regalías por Fonogramas. El Licenciante se reserva el derecho exclusivo de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, SAYCO), una agencia de derechos musicales o algún agente designado, las regalías por cualquier fonograma que Usted cree a partir de la obra (“versión cover”) y distribuya, en los términos del régimen de derechos de autor, si la creación o distribución de esa versión cover está primordialmente destinada o dirigida a obtener una ventaja comercial o una compensación monetaria privada.
e.	Gestión de Derechos de Autor sobre Interpretaciones y Ejecuciones Digitales (WebCasting). Para evitar toda confusión, el Licenciante aclara que, cuando la obra sea un fonograma, el Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública digital de la obra (por ejemplo, webcast) y de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, Acinpro), las regalías por la ejecución pública digital de la obra (por ejemplo, webcast), sujeta a las disposiciones aplicables del régimen de Derecho de Autor, si esta ejecución pública digital está primordialmente dirigida a obtener una ventaja comercial o una compensación monetaria privada.
5. Representaciones, Garantías y Limitaciones de Responsabilidad.
A MENOS QUE LAS PARTES LO ACORDARAN DE OTRA FORMA POR ESCRITO, EL LICENCIANTE OFRECE LA OBRA (EN EL ESTADO EN EL QUE SE ENCUENTRA) “TAL CUAL”, SIN BRINDAR GARANTÍAS DE CLASE ALGUNA RESPECTO DE LA OBRA, YA SEA EXPRESA, IMPLÍCITA, LEGAL O CUALQUIERA OTRA, INCLUYENDO, SIN LIMITARSE A ELLAS, GARANTÍAS DE TITULARIDAD, COMERCIABILIDAD, ADAPTABILIDAD O ADECUACIÓN A PROPÓSITO DETERMINADO, AUSENCIA DE INFRACCIÓN, DE AUSENCIA DE DEFECTOS LATENTES O DE OTRO TIPO, O LA PRESENCIA O AUSENCIA DE ERRORES, SEAN O NO DESCUBRIBLES (PUEDAN O NO SER ESTOS DESCUBIERTOS). ALGUNAS JURISDICCIONES NO PERMITEN LA EXCLUSIÓN DE GARANTÍAS IMPLÍCITAS, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.
6. Limitación de responsabilidad.
A MENOS QUE LO EXIJA EXPRESAMENTE LA LEY APLICABLE, EL LICENCIANTE NO SERÁ RESPONSABLE ANTE USTED POR DAÑO ALGUNO, SEA POR RESPONSABILIDAD EXTRACONTRACTUAL, PRECONTRACTUAL O CONTRACTUAL, OBJETIVA O SUBJETIVA, SE TRATE DE DAÑOS MORALES O PATRIMONIALES, DIRECTOS O INDIRECTOS, PREVISTOS O IMPREVISTOS PRODUCIDOS POR EL USO DE ESTA LICENCIA O DE LA OBRA, AUN CUANDO EL LICENCIANTE HAYA SIDO ADVERTIDO DE LA POSIBILIDAD DE DICHOS DAÑOS. ALGUNAS LEYES NO PERMITEN LA EXCLUSIÓN DE CIERTA RESPONSABILIDAD, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.
7. Término.
a.	Esta Licencia y los derechos otorgados en virtud de ella terminarán automáticamente si Usted infringe alguna condición establecida en ella. Sin embargo, los individuos o entidades que han recibido Obras Derivadas o Colectivas de Usted de conformidad con esta Licencia, no verán terminadas sus licencias, siempre que estos individuos o entidades sigan cumpliendo íntegramente las condiciones de estas licencias. Las Secciones 1, 2, 5, 6, 7, y 8 subsistirán a cualquier terminación de esta Licencia.
b.	Sujeta a las condiciones y términos anteriores, la licencia otorgada aquí es perpetua (durante el período de vigencia de los derechos de autor de la obra). No obstante lo anterior, el Licenciante se reserva el derecho a publicar y/o estrenar la Obra bajo condiciones de licencia diferentes o a dejar de distribuirla en los términos de esta Licencia en cualquier momento; en el entendido, sin embargo, que esa elección no servirá para revocar esta licencia o que deba ser otorgada , bajo los términos de esta licencia), y esta licencia continuará en pleno vigor y efecto a menos que sea terminada como se expresa atrás. La Licencia revocada continuará siendo plenamente vigente y efectiva si no se le da término en las condiciones indicadas anteriormente.
8. Varios.
a.	Cada vez que Usted distribuya o ponga a disposición pública la Obra o una Obra Colectiva, el Licenciante ofrecerá al destinatario una licencia en los mismos términos y condiciones que la licencia otorgada a Usted bajo esta Licencia.
b.	Si alguna disposición de esta Licencia resulta invalidada o no exigible, según la legislación vigente, esto no afectará ni la validez ni la aplicabilidad del resto de condiciones de esta Licencia y, sin acción adicional por parte de los sujetos de este acuerdo, aquélla se entenderá reformada lo mínimo necesario para hacer que dicha disposición sea válida y exigible.
c.	Ningún término o disposición de esta Licencia se estimará renunciada y ninguna violación de ella será consentida a menos que esa renuncia o consentimiento sea otorgado por escrito y firmado por la parte que renuncie o consienta.
d.	Esta Licencia refleja el acuerdo pleno entre las partes respecto a la Obra aquí licenciada. No hay arreglos, acuerdos o declaraciones respecto a la Obra que no estén especificados en este documento. El Licenciante no se verá limitado por ninguna disposición adicional que pueda surgir en alguna comunicación emanada de Usted. Esta Licencia no puede ser modificada sin el consentimiento mutuo por escrito del Licenciante y Usted.

 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