Susceptibilidad de Heliothis virescens (Lepidoptera: noctuidae) a la proteína Cry1Ac incorporada a dietas merídicas
As an alternative for pest management, plants were developed to express lethal proteins against target insects, which may develop resistance due to their constant exposure to these proteins. To monitor changes in susceptibility to Cry1Ac pro-tein and determine the effect exerted by the diet composit...
- Autores:
-
Romero, Laura
Duarte, William
Mejía, Rodolfo
Arévalo Maldonado, Helber Adrian
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2012
- Institución:
- Universidad de Ciencias Aplicadas y Ambientales U.D.C.A
- Repositorio:
- Repositorio Institucional UDCA
- Idioma:
- spa
- OAI Identifier:
- oai:repository.udca.edu.co:11158/1834
- Acceso en línea:
- https://revistas.udca.edu.co/index.php/ruadc/article/view/838
- Palabra clave:
- Bellotero del algodonero
Bacillus thuringiensis
Tolerancia
Bioensayos
Heliothis virescens
Lepidoptera
Noctuidae
- Rights
- openAccess
- License
- Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales
| Summary: | As an alternative for pest management, plants were developed to express lethal proteins against target insects, which may develop resistance due to their constant exposure to these proteins. To monitor changes in susceptibility to Cry1Ac pro-tein and determine the effect exerted by the diet composition on the susceptibility of Heliothis virescens, bioassays were carried out in the U.D.C.A ́s laboratory of Agricultural Bio-technology with populations collected on Desmodium sp., at Espinal, Tolima. The diets employed were ICRISAT, Greene and Shorey & Hale, incorporating serial concentrations of Cry1Ac, ranging from 0.01 to 100 ppm. Bioassays consisted of six treatments and six replicates; each experimental unit was represented by a plastic cup with diet, in which one or five neonate larvae were placed. With the mortality assessed at day seven the lethal concentration (LC50) was calculated. The diet composition influenced the susceptibility response. The LC50 obtained for Heliothis in 2011 (0.956 ppm) indi-cates that this insect lost susceptibility to Cry1Ac expressed by Bollgard®; however, this plant variety still has control over the species. Results also showed that the mortality obtained with five larvae per experimental unit was influenced by can-nibalism and not by the consumption of Cry1Ac, indicating that only one larva per container should be used to eliminate the effect of cannibalism on mortality. |
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