Influence of montane altitudinal ranges on species distribution models; evidence in Andean blow flies

Background. Blow flies are a family of dipterans of medical, veterinary and sanitary importance. We aim to predict the current geographical distribution of six neotropical blowfly species with different altitudinal ranges of distribution (high, medium, and lowlands) and degree of synanthropy (eusyna...

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Autores:
Altamiranda Saavedra, Mariano Augusto
Amat García, Eduardo Carlo
Gómez Piñerez, Luz Miryam
Tipo de recurso:
Article of investigation
Fecha de publicación:
2020
Institución:
Tecnológico de Antioquia
Repositorio:
Repositorio Tdea
Idioma:
eng
spa
OAI Identifier:
oai:dspace.tdea.edu.co:tdea/1142
Acceso en línea:
https://dspace.tdea.edu.co/handle/tdea/1142
Palabra clave:
Regionalization
Human influence index
Species distribution models
Synanthropy
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openAccess
License
Atribución 4.0 Internacional (CC BY 4.0)
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dc.title.spa.fl_str_mv Influence of montane altitudinal ranges on species distribution models; evidence in Andean blow flies
dc.title.alternative.none.fl_str_mv Influencia de los rangos altitudinales montanos en los modelos de distribución de especies; evidencia en moscas del azote andino
title Influence of montane altitudinal ranges on species distribution models; evidence in Andean blow flies
spellingShingle Influence of montane altitudinal ranges on species distribution models; evidence in Andean blow flies
Regionalization
Human influence index
Species distribution models
Synanthropy
title_short Influence of montane altitudinal ranges on species distribution models; evidence in Andean blow flies
title_full Influence of montane altitudinal ranges on species distribution models; evidence in Andean blow flies
title_fullStr Influence of montane altitudinal ranges on species distribution models; evidence in Andean blow flies
title_full_unstemmed Influence of montane altitudinal ranges on species distribution models; evidence in Andean blow flies
title_sort Influence of montane altitudinal ranges on species distribution models; evidence in Andean blow flies
dc.creator.fl_str_mv Altamiranda Saavedra, Mariano Augusto
Amat García, Eduardo Carlo
Gómez Piñerez, Luz Miryam
dc.contributor.author.none.fl_str_mv Altamiranda Saavedra, Mariano Augusto
Amat García, Eduardo Carlo
Gómez Piñerez, Luz Miryam
dc.subject.proposal.spa.fl_str_mv Regionalization
Human influence index
Species distribution models
Synanthropy
topic Regionalization
Human influence index
Species distribution models
Synanthropy
description Background. Blow flies are a family of dipterans of medical, veterinary and sanitary importance. We aim to predict the current geographical distribution of six neotropical blowfly species with different altitudinal ranges of distribution (high, medium, and lowlands) and degree of synanthropy (eusynanthropic, hemisynanthropic and asynanthropic) based on their existing fundamental niche (EA) in Northwestern South America. Methods. Geographical records were compiled based on data from museum specimens and literature. The accessible area hypothesis (M) was calculated based on three criteria: (1) Altitudinal range, (2) Synanthropy values deducted based on the Human Influence Index (HII) raster dataset, and (3). The mean dispersal capability of flies. The modeling was performed using the Maxent entropy modeling software. The selection of parameters was made with the R Program ENMeval package. Results. The models were assessed using the area under the operator-partial receiver curve (ROCp). The high statistical performance was evidenced in every modeling prediction. The modeling allowed identifying possible taxonomic inaccuracies and the lack of exhaustive collection in the field, especially for lowlands species. Geographical distribution predicted by the modeling and empirical data was remarkably coherent in montane species. Discussion. The data obtained evidence that montane elevational ranges affect the performance of the distribution models. These models will allow a more precise predicting of medium and high elevation blow flies than lowlands species. Montane species modeling will accurately predict the fly occurrence to use such biological information for medical, legal, veterinary, and conservation purposes.
publishDate 2020
dc.date.issued.none.fl_str_mv 2020
dc.date.accessioned.none.fl_str_mv 2021-04-26T15:35:41Z
dc.date.available.none.fl_str_mv 2021-04-26T15:35:41Z
dc.type.spa.fl_str_mv Artículo de revista
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dc.relation.citationendpage.spa.fl_str_mv 16
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dc.relation.citationvolume.spa.fl_str_mv 8
dc.relation.ispartofjournal.spa.fl_str_mv PeerJ
dc.relation.citationedition.spa.fl_str_mv Peerj, vol. 8 (2020)
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dc.format.extent.spa.fl_str_mv 16 p.
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dc.coverage.spatial.none.fl_str_mv Noroeste de América del Sur
dc.publisher.spa.fl_str_mv Corte Madera, CA: PeerJ Inc
dc.publisher.place.spa.fl_str_mv Estados Unidos
dc.source.spa.fl_str_mv 10.7717/peerj.10370
institution Tecnológico de Antioquia
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spelling Altamiranda Saavedra, Mariano Augusto5d8b0d76-b2d0-4b05-899c-878403769a5bAmat García, Eduardo Carlo5812d059-3140-4b5a-a528-c1f545c2e71eGómez Piñerez, Luz Miryam61b0a2ae-56c9-4598-83ad-69018c9163d4Noroeste de América del Sur2021-04-26T15:35:41Z2021-04-26T15:35:41Z20202167-8359https://dspace.tdea.edu.co/handle/tdea/1142Background. Blow flies are a family of dipterans of medical, veterinary and sanitary importance. We aim to predict the current geographical distribution of six neotropical blowfly species with different altitudinal ranges of distribution (high, medium, and lowlands) and degree of synanthropy (eusynanthropic, hemisynanthropic and asynanthropic) based on their existing fundamental niche (EA) in Northwestern South America. Methods. Geographical records were compiled based on data from museum specimens and literature. The accessible area hypothesis (M) was calculated based on three criteria: (1) Altitudinal range, (2) Synanthropy values deducted based on the Human Influence Index (HII) raster dataset, and (3). The mean dispersal capability of flies. The modeling was performed using the Maxent entropy modeling software. The selection of parameters was made with the R Program ENMeval package. Results. The models were assessed using the area under the operator-partial receiver curve (ROCp). The high statistical performance was evidenced in every modeling prediction. The modeling allowed identifying possible taxonomic inaccuracies and the lack of exhaustive collection in the field, especially for lowlands species. Geographical distribution predicted by the modeling and empirical data was remarkably coherent in montane species. Discussion. The data obtained evidence that montane elevational ranges affect the performance of the distribution models. These models will allow a more precise predicting of medium and high elevation blow flies than lowlands species. Montane species modeling will accurately predict the fly occurrence to use such biological information for medical, legal, veterinary, and conservation purposes.16 p.application/pdfengspaCorte Madera, CA: PeerJ IncEstados Unidos10.7717/peerj.10370Influence of montane altitudinal ranges on species distribution models; evidence in Andean blow fliesInfluencia de los rangos altitudinales montanos en los modelos de distribución de especies; evidencia en moscas del azote andinoArtículo de revistahttp://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a851618PeerJPeerj, vol. 8 (2020)info:eu-repo/semantics/openAccessAtribución 4.0 Internacional (CC BY 4.0)http://purl.org/coar/access_right/c_abf2RegionalizationHuman influence indexSpecies distribution modelsSynanthropyAmat E. 2009. Contribución al conocimiento de las Chrysomyinae y Toxotarsinae (Diptera: Calliphoridae) de Colombia. Revista Mexicana de Biodiversidad 80:693–708.Amat E. 2017. Calliphoridae (Diptera) do noroeste da América do Sul: Diversidade, distribuicão e código de barras genético. D. Phil. Thesis, Instituto Nacional de ¸ Pesquisas da Amazônia. https://bdtd.inpa.gov.br/handle/tede/2608 (accessed on 21 November 2020).Amat E, Medina CA. 2020. A rapid ecological assessment for necrophagous flies (Diptera, 2020.07.24.22049 Calyptratae) in a mosaic landscape of the Colombian. In Press.Amat E, Vélez MC, Wolff M. 2008. Illustrated key for identification to genera and species of blowflies (Diptera: Calliphoridae) of Colombia. Caldasia 30:231–244 DOI 10.1111/j.1365-3113.2010.00536.x.Amat E, Wolff M. 2007. New records of Blepharicnema splendens (Calliphoridae: Calliphorinae, Luciliini) from Colombia. Revista de la Sociedad Entomológica Argentina 66:187–190.Anderson GS, Cervanka VJ. 2002. Insects associated with the body: their use and analysis. In: Haglund WD, Sorg MH, eds. Adv. Forensic Taphon. Method, Theory, Archeol. Perspect. Boca Raton: CRC Press, 173–200Anderson RP, Lew D, Peterson AT. 2003. Evaluating predictive models of species’ distributions: criteria for selecting optimal models. Ecological Modelling 162:211–232 DOI 10.1016/S0304-3800(02)00349-6.Arias-Robledo G, Stevens JR, Wall R. 2019. Spatial and temporal habitat partitioning by calliphorid blowflies. Medical and Veterinary Entomology.Austin MP. 2002. Spatial prediction of species distribution: an interface between ecological theory and statistical modelling. Ecological Modelling 33(2):228–237 DOI 10.1111/mve.12354.Barve N, Barve V, Jiménez-Valverde A, Lira-Noriega A, Maher SP, Peterson AT, Soberón J, Villalobos F. 2011. The crucial role of the accessible area in ecological niche modeling and species distribution modeling. Ecological Modelling 222:1810–1819 DOI 10.1016/j.ecolmodel.2011.02.011.Basanta MD, Rebollar EA, Parra-Olea G. 2019. Potential risk of Batrachochytrium salamandrivorans in Mexico. PLOS ONE 14:1–13 DOI 10.1371/journal.pone.0211960.Baumgartner D. 1988. Spread of introduced Chrysomya blow flies (Diptera: Calliphoridae) in Neotropics with records new to Venezuela. Biotropica 20:167–168 DOI 10.2307/2388191.Baumgartner DL, Greenberg B. 1985. Distribution and medical ecology of the blow flies (Diptera: Calliphoridae) of Peru1. Annals of the Entomological Society of America 78:565–587 DOI 10.1093/aesa/78.5.565.Bishopp FC, Laake EW. 1921. Dispersal of flies by flight. Journal of Agricultural Research 21:726–766.Dear JP. 1979. Revision of the Toxotarsinae (Diptera: Calliphoridae). Papeis Avulsos de Zoologia 32(13):145–182.Dear JP. 1985. A revision of the New World Chrysomyini (Diptera: Calliphoridae). Revista Brasileira de Zoologia 3:109–169 DOI 10.1590/S0101-81751985000300001.Elith J, Leathwick JR. 2009. Species distribution models: ecological explanation and prediction across space and time. Annual Review of Ecology, Evolution, and Systematics 40:677–697 DOI 10.1146/annurev.ecolsys.110308.120159.Fick SE, Hijmans RJ. 2017. Worldclim 2: new 1-km spatial resolution climate surfaces for global land areas. International Journal of Climatology 9:4302–4315.Francesconi F, Lupi O. 2012. Myiasis. Clinical Microbiology Reviews 25(1):79 LP–105 DOI 10.1128/CMR.00010-11.Galante E, Marcos-Garcia MA. 1997. Detritívoros, Coprófagos y Necrófagos. Boletín de la Sociedad Entomológica Aragonesa 20:57–64.Greco S, Brandmayr P, Bonacci T. 2014. Synanthropy and temporal variability of calliphoridae living in Cosenza (Calabria, Southern Italy). Journal of Insect Science 14(1):216 DOI 10.1093/jisesa/ieu078.Greenberg B. 1973. Flies and disease II: biology and disease transmission. Princeton: Princeton University Press.Greenberg B, Kunich JC. 2002. Entomology and the law: flies as forensic indicators. Cambridge: Cambridge University Press.Gregor F, Povolný D. 1958. Versuch einer Klassifikation der synanthropen fliegen (Diptera). Journal Hygiene Epidemiology Microbiology & Inmunology 2:205–216.216.Hall DJ. 1948. The blowflies of North America. Lafayette: Thomas Say Foundation.Hodkinson ID. 2005. Terrestrial insects along elevation gradients: species and community responses to altitude. Biological Reviews of the Cambridge Philosophical Society 80:489–513 DOI 10.1017/S1464793105006767.Hwang C, Turner BD. 2005. Spatial and temporal variability of necrophagous Diptera from urban to rural areas. Medical and Veterinary Entomology 19:379–391 DOI 10.1111/j.1365-2915.2005.00583.x.James MT. 1970. Family Calliphoridae. In: Papavero N, ed. A catalogue of the diptera of Americas south of the United States. São Paulo: Museu de Zoologia, Universidade de São Paulo, 28.Kavazos CRJ, Wallman JF. 2012. Community composition of carrion-breeding blowflies (Diptera: Calliphoridae) along an urban gradient in south-eastern Australia. Landscape and Urban Planning 106:183–190 DOI 10.1016/j.landurbplan.2012.03.002.Kosmann C, Mello RP de, Harterreiten-Souza ÉS, Pujol-Luz JR. 2013. A list of current valid blow fly names (Diptera: Calliphoridae) in the Americas South ofMexico with key to the Brazilian species. EntomoBrasilis 6:74–85 DOI 10.12741/ebrasilis.v6i1.266.Lecheta MC, Corrêa RC, Moura MO. 2017. Climate shapes the geographic distribution of the blowfly Sarconesia chlorogaster (Diptera: Calliphoridae): an environmental niche modeling approach. Environmental Entomology 46:1051–1059 DOI 10.1093/ee/nvx124.Lindquist AW, Yates WW, Hoffman RA, Butts JS. 1951. Studies of the flight habits of three species of flies tagged with radioactive phosphorus1. Journal of Economic Entomology 44:397–400 DOI 10.1093/jee/44.3.397.Mani MS. 1968. Ecological specializations of high altitude insects. In: Ecology and biogeography of high altitude insects. Dordrecht: Springer Netherlands, 51–74 DOI 10.1007/978-94-017-1339-9_3.Martínez-Sánchez A, Rojo S, Marcos-García MA. 2000. Annual and spatial activity of dung flies and carrion in a Mediterranean holm-oak pasture ecosystem. Medical and Veterinary Entomology 14:56–63 DOI 10.1046/j.1365-2915.2000.00205.x.Montoya GA, Sanchez JD, Wolff M. 2009. Sinantropía de Calliphoridae (Diptera) del Municipio La Pintada, Antioquia—Colombia. Revista Colombiana de Entomologia 35:73–82.Mulieri PR, Patitucci LD. 2019. Using ecological niche models to describe the geographical distribution of the myiasis-causing Cochliomyia hominivorax (Diptera: Calliphoridae) in southern South America. Parasitology Research 118:1077–1086 DOI 10.1007/s00436-019-06267-0.Muscarella R, Galante PJ, Soley-Guardia M, Boria RA, Kass JM, Uriarte M, Anderson RP. 2014. ENMeval: an R package for conducting spatially independent evaluations and estimating optimal model complexity for Maxent ecological niche models. Methods in Ecology and Evolution 5:1198–1205 DOI 10.1111/2041-210X.12261.Norris KR. 1965. The bionomics of blow flies. Annual Review of Entomology 10:47–68 DOI 10.1146/annurev.en.10.010165.000403.Nuorteva P. 1963. Synanthropy of blowflies (Dipt., Calliphoridae) in Finland. Annales Entomologicae Fennicae 29:1–49.Osorio-Olvera L, Lira-Noriega A, Soberón J, Peterson AT, Falconi M, Contreras-Díaz RG, Barve V, Barve N. 2020. ntbox: an R package with graphical user interface for modeling and evaluating multidimensional ecological niches. Methods in Ecology and Evolution 2041–210X.13452 DOI 10.1111/2041-210X.13452.Pape T, Blagoderov V, Mostovski MB. 2011. Order Diptera Linnaeus, 1758. In: Zhang ZQ, ed. Animal biodiversity: an outline of higher-level classification and survey of taxonomic richness. Zootaxa. vol. 3148. 222–229.Pearson RG, Raxworthy CJ, Nakamura M, Townsend Peterson A. 2006. Predicting species distributions from small numbers of occurrence records: a test case using cryptic geckos in Madagascar. Journal of Biogeography 34:102–117 DOI 10.1111/j.1365-2699.2006.01594.x.Peterson AT, Papes M, Sober J. 2008. Rethinking receiver operating characteristic analysis applications in ecological niche modeling. Ecological Modelling 3:63–72 DOI 10.1016/j.ecolmodel.2007.11.008.Peterson AT, Sánchez-cordero V, Ben BC, Ramsey JM. 2002. Ecologic niche modeling and potential reservoirs for chagas disease, Mexico. Emerging Infectious Diseases 8(7):662–667 DOI 10.3201/eid0807.010454.Peterson AT, Soberon J, Pearson RG, Anderson RP, Martinez-Meyer E, Nakamura M, Araujo MB. 2011. Ecological niches and geographic distributions. Princeton University Press DOI 10.23943/princeton/9780691136868.001.0001.Peterson AT, Soberón J, Ramsey J, Osorio-Olvera L. 2020. Co-occurrence networks do not support identification of biotic interactions. Biodiversity Informatics 15:1–10 DOI 10.17161/bi.v15i1.9798.Phillips SJ, Anderson RP, Schapire RE. 2006. Maximum entropy modeling of species geographic distributions. Ecological Modelling 190:231–259 DOI 10.1016/j.ecolmodel.2005.03.026.Pinilla-Beltran YT, Segura NA, Bello FJ. 2012. Synanthropy of Calliphoridae and Sarcophagidae (Diptera) in Bogotá, Colombia. Neotropical Entomology 41(3):237–242 DOI 10.1007/s13744-012-0036-x.Povolný D. 1971. Synanthropy. In: Greenberg B, ed. Flies and disease, ecology, classification, and biotic associations, 1. Princeton: Princeton University Press 17–54.Qiao H, Peterson AT, Campbell LP, Soberón J, Ji L, Escobar LE. 2016b. NicheA: creating virtual species and ecological niches in multivariate environmental scenarios. Ecography 39:805–813 DOI 10.1111/ecog.01961.Qiao H, Saupe EE, Soberón J, Peterson AT, Myers CE. 2016a. Impacts of Niche Breadth and Dispersal Ability on Macroevolutionary Patterns. The American Naturalist 188(2):149–162 DOI 10.1086/687201.Quarterman KD, Mathis W, Kilpatrick JW. 1954. Urban fly dispersal in the area of Savannah, Georgia. Journal of Economic Entomology 47:405–412 DOI 10.1093/jee/47.3.405.Rabus B, Eineder M, Roth A, Bamler R. 2003. The shuttle radar topography mission—a new class of digital elevation models acquired by spaceborne radar. Journal of Photogrammetry and Remote Sensing 57:241–262 DOI 10.1016/S0924-2716(02)00124-7.Raghavan RK, Barker SC, Cobos ME, Barker D, Teo EJM, Foley DH, Nakao R, Lawrence K, Heath ACG, Peterson AT. 2019. Potential spatial distribution of the newly introduced long-horned tick, Haemaphysalis longicornis in North America. Scientific Reports 9:498 DOI 10.1038/s41598-018-37205-2.Richards CS, Williams KA, Villet MH. 2009. Predicting geographic distribution of seven forensically significant blowfly species (Diptera: Calliphoridae) in South Africa. African Entomology 17:170–182 DOI 10.4001/003.017.0207.Rognes K. 1997. The Calliphoridae (Blowflies) (Diptera: Oestroidea) are not a monophyletic group. Cladistics 13:27–66 DOI 10.1006/clad.1997.0031.Rognes T, Whitworth T. 2012. Identification of Neotropical blow flies of the genus Calliphora Robineau- Desvoidy (Diptera: Calliphoridae) with the description of a new species. Zootaxa 3209:1–27 DOI 10.11646/zootaxa.3209.1.1.Shannon RC. 1926. Synopsis of the American Calliphoridae (Diptera). Proceedings of the Entomological Society of Washington 28:115–139.Shura-Bura EV, Shaikow AD, Ivanova EV, Gazunova AL, Mitruikova MS, Fedorova KG. 1958. The character of dispersion from the point of release in certain species of flies of medical importance. Entomologie Obozrenie 37:336–346.Smith KE, Wall R. 1997. The use of carrion as breeding sites by the blowfly Lucilia sericata and other Calliphoridae. Medical and Veterinary Entomology 11:38–44 DOI 10.1111/j.1365-2915.1997.tb00287.x.Velásquez Y, Martínez-Sánchez AI, Thomas A, Rojo S. 2017. Checklist and distribution maps of the blow flies of Venezuela (Diptera, Calliphoridae, Mesembrinellidae). ZooKeys 645:103–132 DOI 10.3897/zookeys.645.6972.Whitworth T. 2014. A revision of the Neotropical species of Lucilia Robineau-Desvoidy (Diptera: Calliphoridae). Zootaxa 3810:76 DOI 10.11646/zootaxa.3810.1.1.Wildlife Conservation Society WCS, University C for IESIN-C-C. 2005. Last of the Wild Project, Version 2, 2005 (LWP-2): Global Human Influence Index (HII) Dataset (Geographic). 38108(1):001–076.Williams KA, Richards CS, Villet MH. 2014. Predicting the geographic distribution of Lucilia sericata and Lucilia cuprina (Diptera: Calliphoridae) in South Africa. African Invertebrates 55:157–170 DOI 10.5733/afin.055.0109.Wolff M, Kosmann C. 2016. Families Calliphoridae and Mesembrinellidae. Zootaxa 4122:856–875 DOI 10.11646/zootaxa.4122.1.7.Yan G, Liu S, Schlink AC, Flematti GR, Brodie BS, Bohman B, Greeff JC, Vercoe PE, Hu J, Martin GB. 2018. Behavior and electrophysiological response of gravid and nongravid Lucilia cuprina (Diptera: Calliphoridae) to carrion-associated compounds. Journal of Economic Entomology 111(4):1958–1965 DOI 10.1093/jee/toy115.Yusseff-Vanegas SZ, Agnarsson I. 2017. DNA-barcoding of forensically important blow flies (Diptera: Calliphoridae) in the Caribbean region. PeerJ 5:e3516 DOI 10.7717/peerj.3516.Zabala J, Díaz B, Saloña Bordas MI. 2014. Seasonal blowfly distribution and abundance in fragmented landscapes. Is it useful in forensic inference about where a corpse has been decaying? PLOS ONE 9(6):e99668 DOI 10.1371/journal.pone.0099668.ORIGINALpeerj-10370.pdfpeerj-10370.pdfapplication/pdf3632260https://dspace.tdea.edu.co/bitstream/tdea/1142/1/peerj-10370.pdf335eda5351c7c3097d4f06be9b6cddccMD51open accessLICENSElicense.txtlicense.txttext/plain; charset=utf-814828https://dspace.tdea.edu.co/bitstream/tdea/1142/2/license.txt2f9959eaf5b71fae44bbf9ec84150c7aMD52open accessTEXTpeerj-10370.pdf.txtpeerj-10370.pdf.txtExtracted texttext/plain48603https://dspace.tdea.edu.co/bitstream/tdea/1142/3/peerj-10370.pdf.txtc84f6d347337603cb989ff2e9c613be4MD53open accessTHUMBNAILpeerj-10370.pdf.jpgpeerj-10370.pdf.jpgGenerated Thumbnailimage/jpeg16044https://dspace.tdea.edu.co/bitstream/tdea/1142/4/peerj-10370.pdf.jpgfcec1e3bc239a49a1eff29dd21f92859MD54open accesstdea/1142oai:dspace.tdea.edu.co:tdea/11422021-05-16 15:56:28.927open accessRepositorio Institucional Tecnologico de 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 incorporada en las Obras Colectivas.

b.	Distribuir copias o fonogramas de las Obras, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública, incluyéndolas como incorporadas en Obras Colectivas, según corresponda.

c.	Distribuir copias de las Obras Derivadas que se generen, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública.
Los derechos mencionados anteriormente pueden ser ejercidos en todos los medios y formatos, actualmente conocidos o que se inventen en el futuro. Los derechos antes mencionados incluyen el derecho a realizar dichas modificaciones en la medida que sean técnicamente necesarias para ejercer los derechos en otro medio o formatos, pero de otra manera usted no está autorizado para realizar obras derivadas. Todos los derechos no otorgados expresamente por el Licenciante quedan por este medio reservados, incluyendo pero sin limitarse a aquellos que se mencionan en las secciones 4(d) y 4(e).

4. Restricciones.
La licencia otorgada en la anterior Sección 3 está expresamente sujeta y limitada por las siguientes restricciones:

a.	Usted puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra sólo bajo las condiciones de esta Licencia, y Usted debe incluir una copia de esta licencia o del Identificador Universal de Recursos de la misma con cada copia de la Obra que distribuya, exhiba públicamente, ejecute públicamente o ponga a disposición pública. No es posible ofrecer o imponer ninguna condición sobre la Obra que altere o limite las condiciones de esta Licencia o el ejercicio de los derechos de los destinatarios otorgados en este documento. No es posible sublicenciar la Obra. Usted debe mantener intactos todos los avisos que hagan referencia a esta Licencia y a la cláusula de limitación de garantías. Usted no puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra con alguna medida tecnológica que controle el acceso o la utilización de ella de una forma que sea inconsistente con las condiciones de esta Licencia. Lo anterior se aplica a la Obra incorporada a una Obra Colectiva, pero esto no exige que la Obra Colectiva aparte de la obra misma quede sujeta a las condiciones de esta Licencia. Si Usted crea una Obra Colectiva, previo aviso de cualquier Licenciante debe, en la medida de lo posible, eliminar de la Obra Colectiva cualquier referencia a dicho Licenciante o al Autor Original, según lo solicitado por el Licenciante y conforme lo exige la cláusula 4(c).

b.	Usted no puede ejercer ninguno de los derechos que le han sido otorgados en la Sección 3 precedente de modo que estén principalmente destinados o directamente dirigidos a conseguir un provecho comercial o una compensación monetaria privada. El intercambio de la Obra por otras obras protegidas por derechos de autor, ya sea a través de un sistema para compartir archivos digitales (digital file-sharing) o de cualquier otra manera no será considerado como estar destinado principalmente o dirigido directamente a conseguir un provecho comercial o una compensación monetaria privada, siempre que no se realice un pago mediante una compensación monetaria en relación con el intercambio de obras protegidas por el derecho de autor.

c.	Si usted distribuye, exhibe públicamente, ejecuta públicamente o ejecuta públicamente en forma digital la Obra o cualquier Obra Derivada u Obra Colectiva, Usted debe mantener intacta toda la información de derecho de autor de la Obra y proporcionar, de forma razonable según el medio o manera que Usted esté utilizando: (i) el nombre del Autor Original si está provisto (o seudónimo, si fuere aplicable), y/o (ii) el nombre de la parte o las partes que el Autor Original y/o el Licenciante hubieren designado para la atribución (v.g., un instituto patrocinador, editorial, publicación) en la información de los derechos de autor del Licenciante, términos de servicios o de otras formas razonables; el título de la Obra si está provisto; en la medida de lo razonablemente factible y, si está provisto, el Identificador Uniforme de Recursos (Uniform Resource Identifier) que el Licenciante especifica para ser asociado con la Obra, salvo que tal URI no se refiera a la nota sobre los derechos de autor o a la información sobre el licenciamiento de la Obra; y en el caso de una Obra Derivada, atribuir el crédito identificando el uso de la Obra en la Obra Derivada (v.g., "Traducción Francesa de la Obra del Autor Original," o "Guión Cinematográfico basado en la Obra original del Autor Original"). Tal crédito puede ser implementado de cualquier forma razonable; en el caso, sin embargo, de Obras Derivadas u Obras Colectivas, tal crédito aparecerá, como mínimo, donde aparece el crédito de cualquier otro autor comparable y de una manera, al menos, tan destacada como el crédito de otro autor comparable.

d.	Para evitar toda confusión, el Licenciante aclara que, cuando la obra es una composición musical:

i.	Regalías por interpretación y ejecución bajo licencias generales. El Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública o la ejecución pública digital de la obra y de recolectar, sea individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, SAYCO), las regalías por la ejecución pública o por la ejecución pública digital de la obra (por ejemplo Webcast) licenciada bajo licencias generales, si la interpretación o ejecución de la obra está primordialmente orientada por o dirigida a la obtención de una ventaja comercial o una compensación monetaria privada.

ii.	Regalías por Fonogramas. El Licenciante se reserva el derecho exclusivo de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, los consagrados por la SAYCO), una agencia de derechos musicales o algún agente designado, las regalías por cualquier fonograma que Usted cree a partir de la obra (“versión cover”) y distribuya, en los términos del régimen de derechos de autor, si la creación o distribución de esa versión cover está primordialmente destinada o dirigida a obtener una ventaja comercial o una compensación monetaria privada.

e.	Gestión de Derechos de Autor sobre Interpretaciones y Ejecuciones Digitales (WebCasting). Para evitar toda confusión, el Licenciante aclara que, cuando la obra sea un fonograma, el Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública digital de la obra (por ejemplo, webcast) y de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, ACINPRO), las regalías por la ejecución pública digital de la obra (por ejemplo, webcast), sujeta a las disposiciones aplicables del régimen de Derecho de Autor, si esta ejecución pública digital está primordialmente dirigida a obtener una ventaja comercial o una compensación monetaria privada.

5. Representaciones, Garantías y Limitaciones de Responsabilidad.
A MENOS QUE LAS PARTES LO ACORDARAN DE OTRA FORMA POR ESCRITO, EL LICENCIANTE OFRECE LA OBRA (EN EL ESTADO EN EL QUE SE ENCUENTRA) “TAL CUAL”, SIN BRINDAR GARANTÍAS DE CLASE ALGUNA RESPECTO DE LA OBRA, YA SEA EXPRESA, IMPLÍCITA, LEGAL O CUALQUIERA OTRA, INCLUYENDO, SIN LIMITARSE A ELLAS, GARANTÍAS DE TITULARIDAD, COMERCIABILIDAD, ADAPTABILIDAD O ADECUACIÓN A PROPÓSITO DETERMINADO, AUSENCIA DE INFRACCIÓN, DE AUSENCIA DE DEFECTOS LATENTES O DE OTRO TIPO, O LA PRESENCIA O AUSENCIA DE ERRORES, SEAN O NO DESCUBRIBLES (PUEDAN O NO SER ESTOS DESCUBIERTOS). ALGUNAS JURISDICCIONES NO PERMITEN LA EXCLUSIÓN DE GARANTÍAS IMPLÍCITAS, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.

6. Limitación de responsabilidad.
A MENOS QUE LO EXIJA EXPRESAMENTE LA LEY APLICABLE, EL LICENCIANTE NO SERÁ RESPONSABLE ANTE USTED POR DAÑO ALGUNO, SEA POR RESPONSABILIDAD EXTRACONTRACTUAL, PRECONTRACTUAL O CONTRACTUAL, OBJETIVA O SUBJETIVA, SE TRATE DE DAÑOS MORALES O PATRIMONIALES, DIRECTOS O INDIRECTOS, PREVISTOS O IMPREVISTOS PRODUCIDOS POR EL USO DE ESTA LICENCIA O DE LA OBRA, AUN CUANDO EL LICENCIANTE HAYA SIDO ADVERTIDO DE LA POSIBILIDAD DE DICHOS DAÑOS. ALGUNAS LEYES NO PERMITEN LA EXCLUSIÓN DE CIERTA RESPONSABILIDAD, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.

7. Término.

a.	Esta Licencia y los derechos otorgados en virtud de ella terminarán automáticamente si Usted infringe alguna condición establecida en ella. Sin embargo, los individuos o entidades que han recibido Obras Derivadas o Colectivas de Usted de conformidad con esta Licencia, no verán terminadas sus licencias, siempre que estos individuos o entidades sigan cumpliendo íntegramente las condiciones de estas licencias. Las Secciones 1, 2, 5, 6, 7, y 8 subsistirán a cualquier terminación de esta Licencia.

b.	Sujeta a las condiciones y términos anteriores, la licencia otorgada aquí es perpetua (durante el período de vigencia de los derechos de autor de la obra). No obstante lo anterior, el Licenciante se reserva el derecho a publicar y/o estrenar la Obra bajo condiciones de licencia diferentes o a dejar de distribuirla en los términos de esta Licencia en cualquier momento; en el entendido, sin embargo, que esa elección no servirá para revocar esta licencia o que deba ser otorgada , bajo los términos de esta licencia), y esta licencia continuará en pleno vigor y efecto a menos que sea terminada como se expresa atrás. La Licencia revocada continuará siendo plenamente vigente y efectiva si no se le da término en las condiciones indicadas anteriormente.

8. Varios.

a.	Cada vez que Usted distribuya o ponga a disposición pública la Obra o una Obra Colectiva, el Licenciante ofrecerá al destinatario una licencia en los mismos términos y condiciones que la licencia otorgada a Usted bajo esta Licencia.

b.	Si alguna disposición de esta Licencia resulta invalidada o no exigible, según la legislación vigente, esto no afectará ni la validez ni la aplicabilidad del resto de condiciones de esta Licencia y, sin acción adicional por parte de los sujetos de este acuerdo, aquélla se entenderá reformada lo mínimo necesario para hacer que dicha disposición sea válida y exigible.

c.	Ningún término o disposición de esta Licencia se estimará renunciada y ninguna violación de ella será consentida a menos que esa renuncia o consentimiento sea otorgado por escrito y firmado por la parte que renuncie o consienta.

d.	Esta Licencia refleja el acuerdo pleno entre las partes respecto a la Obra aquí licenciada. No hay arreglos, acuerdos o declaraciones respecto a la Obra que no estén especificados en este documento. El Licenciante no se verá limitado por ninguna disposición adicional que pueda surgir en alguna comunicación emanada de Usted. Esta Licencia no puede ser modificada sin el consentimiento mutuo por escrito del Licenciante y Usted.
