Mycorrhizae: a key interaction for conservation of two endangered Magnolias from Andean forests
Background and aims – Magnolia species are highly endangered in neotropical forests where they are highly endemic and often very rare. However, little is known about their nutritional and soil conditions in natural forests. In this study, we focused on two endangered Magnolia species that cohabit in...
- Autores:
-
Serna González, Llinet Marcela
Urrego Giraldo, Ligia Estela
Osorio Vega, Nelson Walter
Valencia Ríos, Diego
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2019
- Institución:
- Tecnológico de Antioquia
- Repositorio:
- Repositorio Tdea
- Idioma:
- eng
- OAI Identifier:
- oai:dspace.tdea.edu.co:tdea/2825
- Acceso en línea:
- https://dspace.tdea.edu.co/handle/tdea/2825
- Palabra clave:
- Conservation
Conservação
Conservación
Rhizosphere
Rizosfera
Rizosfera
Soil fertility
Fertilidade do solo
Fertilidad del suelo
Mycorrhizae
Andean cloud forests
Magnolia jardinensis
Magnolia yarumalensis
Tree nutrition
- Rights
- openAccess
- License
- https://creativecommons.org/licenses/by/4.0/
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Mycorrhizae: a key interaction for conservation of two endangered Magnolias from Andean forests |
| title |
Mycorrhizae: a key interaction for conservation of two endangered Magnolias from Andean forests |
| spellingShingle |
Mycorrhizae: a key interaction for conservation of two endangered Magnolias from Andean forests Conservation Conservação Conservación Rhizosphere Rizosfera Rizosfera Soil fertility Fertilidade do solo Fertilidad del suelo Mycorrhizae Andean cloud forests Magnolia jardinensis Magnolia yarumalensis Tree nutrition |
| title_short |
Mycorrhizae: a key interaction for conservation of two endangered Magnolias from Andean forests |
| title_full |
Mycorrhizae: a key interaction for conservation of two endangered Magnolias from Andean forests |
| title_fullStr |
Mycorrhizae: a key interaction for conservation of two endangered Magnolias from Andean forests |
| title_full_unstemmed |
Mycorrhizae: a key interaction for conservation of two endangered Magnolias from Andean forests |
| title_sort |
Mycorrhizae: a key interaction for conservation of two endangered Magnolias from Andean forests |
| dc.creator.fl_str_mv |
Serna González, Llinet Marcela Urrego Giraldo, Ligia Estela Osorio Vega, Nelson Walter Valencia Ríos, Diego |
| dc.contributor.author.none.fl_str_mv |
Serna González, Llinet Marcela Urrego Giraldo, Ligia Estela Osorio Vega, Nelson Walter Valencia Ríos, Diego |
| dc.subject.other.none.fl_str_mv |
Conservation Conservação Conservación |
| topic |
Conservation Conservação Conservación Rhizosphere Rizosfera Rizosfera Soil fertility Fertilidade do solo Fertilidad del suelo Mycorrhizae Andean cloud forests Magnolia jardinensis Magnolia yarumalensis Tree nutrition |
| dc.subject.agrovoc.none.fl_str_mv |
Rhizosphere Rizosfera Rizosfera Soil fertility Fertilidade do solo Fertilidad del suelo Mycorrhizae |
| dc.subject.proposal.none.fl_str_mv |
Andean cloud forests Magnolia jardinensis Magnolia yarumalensis Tree nutrition |
| description |
Background and aims – Magnolia species are highly endangered in neotropical forests where they are highly endemic and often very rare. However, little is known about their nutritional and soil conditions in natural forests. In this study, we focused on two endangered Magnolia species that cohabit in the Colombian Andean cloud forests in order to identify their conservation and nutritional status. We hypothesize that these species might exhibit mycorrhizal colonization that enhance nutrients uptake in poor and disturbed soils. Methods – Individuals of Magnolia jardinensis and M. yarumalensis were assessed in 11 000 m2 of Andean forests remnants from Jardín municipality (Antioquia, Colombia). Foliar and soil samples were analysed in the lab. Through a Principal Component Analysis (PCA) we identified the relationship between soil conditions and foliar nutrition. Root fragments and rhizosphere samples from seedlings and juveniles up to 3 m tall were collected to verify mycorrhizal colonization and presence of other microorganisms. Adults were excluded of the sampling due to the difficulties to differentiate their roots among the rest of the species in the forest fragments. Key results – The surveys show that the M. yarumalensis population has an inverted J-shaped diametric distribution suggesting a potential recovering population while the smaller overall distribution of M. jardinensis in all diametric categories suggests that this species is likely to become extinct. Both species grow in acidic, infertile soils, although foliar nutrient concentrations did not correlate with soil-nutrient availability. Such a discrepancy and the high colonization levels of mycorrhizae (60–70%) and dark septate endophytes (40–45%), suggest that plant-microorganisms may facilitate nutrition and enhance survival of Magnolia species in stressed environments. Other fungi and bacteria were also found in their rhizosphere, but their role with respect to Magnolia species remains unclear. Conclusions - Mycorrhizal colonization of endangered Magnolia species seems to play a key role to their performance in natural disturbed Andean forests. Aspects related to soil and rhizosphere ecology should be included in conservation projects for endangered and endemic plants. Key words – Rhizosphere, Andean cloud forests, Magnolia jardinensis, Magnolia yarumalensis, soil fertility, tree nutrition, conservation, mycorrhizae. |
| publishDate |
2019 |
| dc.date.issued.none.fl_str_mv |
2019 |
| dc.date.accessioned.none.fl_str_mv |
2023-04-26T00:04:47Z |
| dc.date.available.none.fl_str_mv |
2023-04-26T00:04:47Z |
| dc.type.spa.fl_str_mv |
Artículo de revista |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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Text |
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info:eu-repo/semantics/article |
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http://purl.org/redcol/resource_type/ART |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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publishedVersion |
| dc.identifier.issn.spa.fl_str_mv |
2032-3913 |
| dc.identifier.uri.none.fl_str_mv |
https://dspace.tdea.edu.co/handle/tdea/2825 |
| dc.identifier.eissn.spa.fl_str_mv |
2032-3921 |
| identifier_str_mv |
2032-3913 2032-3921 |
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https://dspace.tdea.edu.co/handle/tdea/2825 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.citationendpage.spa.fl_str_mv |
40 |
| dc.relation.citationissue.spa.fl_str_mv |
1 |
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30 |
| dc.relation.citationvolume.spa.fl_str_mv |
152 |
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Plant ecology and evolution |
| dc.relation.references.spa.fl_str_mv |
Alarcón A.L. (2001) El boro como nutriente esencial. Revista Horticultura 155: 1–11. Alemañy-Merly S.E. (1999) Magnolia portoricensis Bello. New Orleans, Department of Agriculture, Forest Service, Southern Forest Experiment Station Andersen K.M., Turner B.L., Dalling J.W. (2010) Soil-based habitat partitioning in understorey palms in lower montane tropical forests. Journal of Biogeography 37: 278–292. https://doi. org/10.1111/j.1365-2699.2009.02192.x Armenteras D., Rodríguez N., Retana J., Morales M. (2011) Understanding deforestation in montane and lowland forests of the Colombian Andes. Regional Environmental Change 11: 693– 705. https://doi.org/10.1007/s10113-010-0200-y Barrow J.R., Osuna P. (2002) Phosphorus solubilization and uptake by dark septate fungi in fourwing saltbush, Atriplex canescens (Pursh) Nutt. Journal of Arid Environments 51: 449–459. https:// doi.org/10.1006/jare.2001.0925 Becker P., Castillo A. (1990) Root architecture of shrubs and saplings in the understory of a tropical moist forest in lowland Panama. Biotropica 22: 242–249. https://doi.org/10.2307/2388534 Bleish L., Xie J. (1998) Selected nutrient analysis of plants in the diet of the Guizhou snub-nosed monkey (Rhinopithecus brelkichi). In: Jablonski N (ed.) The natural history of the doucs and snub-nosed monkeys: 252. London, World Scientific Blinn C., Bucker E. (1989) Normal foliar nutrient levels in North American forest trees Station Bulletin 590-1989 (Item No. ADSB- 3762). St Paul, University of Minnesota. Bruijnzeel L.A. (1991) Nutrient input-output budgets of tropical forest ecosystems: a review. Journal of Tropical Ecology 7: 1–24. https://doi.org/10.1017/S0266467400005010 Cámara-Leret R., Tuomisto H., Ruokolainen K., Balslev H., Munch Kristiansen S. (2017) Modelling responses of western Amazonian palms to soil nutrients. Journal of Ecology 105: 367–381. https://doi.org/10.1111/1365-2745.12708 Cano M.A. (2011) Interacción de microorganismos benéficos en plantas: Micorrizas, Trichoderma spp. y Pseudomonas spp. Una revisión. Revista U.D.C.A. Actualidad & Divulgación Científica 14: 15–31. Castro F. (1996) Base técnica para el conocimiento y manejo del suelo del Valle del Alto Magdalena. Espinal, CORPOICA. Chen H., Gurmesa G.A., Liu L., Zhang T., Fu S., Liu Z., Zhanfeng L., Shaofeng D., Chuan M., Jianming M. (2014) Effects of litter manipulation on litter decomposition in a successional gradients of tropical forests in southern China. PLOS ONE 9: e99018. https://doi.org/10.1371/journal.pone.0099018 Clark D. (2002) Los factores edáficos y la distribución de las plantas. In: Guariguatta M., Kattan G. (eds) Ecología y conservación de bosques neotropicales: 193–222. Cartago, Ediciones LUR. CORANTIOQUIA (2011) Avances en la estrategia para la conservación de la familia Magnoliaceae en jurisdicción de CORANTIOQUIA. Boletín Técnico Biodiversidad 6: 100–101. Dalpé Y., Séguin S.M. (2013) Microwave-assisted technology for the clearing and staining of arbuscular mycorrhizal fungi in roots. Mycorrhiza 23: 333–340. https://doi.org/10.1007/s00572- 012-0472-9 de Oliveira Freitas R., Buscardo E., Nagy L., dos Santos Maciel A.B., Carrenho R. Luizão R.C.C. (2014) Arbuscular mycorrhizal fungal communities along a pedo-hydrological gradient in a Central Amazonian terra firme forest. Mycorrhiza 24: 21–32. https://doi.org/10.1007/s00572-013-0507-x de Souza R., Ambrosini A., Passaglia L.M.P. (2015) Plant growthpromoting bacteria as inoculants in agricultural soils. Genetics and Molecular Biology 38: 401–419. https://doi.org/10.1590/ S1415-475738420150053 Espinoza L., Slaton N., Mozaffari M. (2000) Como interpretar los resultados de los análisis de suelos. Agricultura y Recursos Naturales FSA2118SP. Little Rock, University of Arkansas. Etter A., McAlpine C., Wilson K., Phinn S., Possingham H. (2006) Regional patterns of agricultural land use and deforestation in Colombia. Agriculture, Ecosystems & Environment 114: 369– 386. https://doi.org/10.1016/j.agee.2005.11.013 Fitter A.H., Helgason T., Hodge A. (2011) Nutritional exchanges in the arbuscular mycorrhizal symbiosis: implications for sustainable agriculture. Fungal Biology Reviews 25: 68–72. https:// doi.org/10.1016/j.fbr.2011.01.002 Garcés de Granada E., Martha O.D.A., Bautista G.R., Valencia H. (2001) Fusarium oxysporum el hongo que nos falta conocer. Acta Biológica Colombiana 6: 7–26. Giovannetti M., Mosse B. (1980) An evaluation of techniques for measuring vesicular arbuscular mycorrhizal infection in roots. New Phytologist 84: 489–500. https://doi. org/10.1111/j.1469-8137.1980.tb04556.x Girmé G., Grau E., Calvo M.A., Leonardo E. (2014) Clave dicotómica para la identificación de hongos aislados sistemáticamente en ambientes mediterráneos. Revista de La Sociedad Española de Microbiología 57: 69–71. Grilli G., Urcelay C., Galetto L. (2013) Linking mycorrhizal fungi and soil nutrients to vegetative and reproductive ruderal plant development in a fragmented forest at central Argentina. Forest Ecology and Management 310: 442–449. https://doi. org/10.1016/j.foreco.2013.08.052 Gutierrez L., Vovides A.P. (1997) An in situ study of Magnolia dealbata Zucc. in Veracruz State: an endangered endemic tree of Mexico. Biodiversity & Conservation 6: 89–97. http://doi. org/10.1023/A%3A1018327700030 Heineman K.D., Caballero P., Morris A., Velasquez C., Serrano K., Ramos N., Gonzalez J., Mayorga L., Corre M.D., Dalling J.W. (2015) Variation in canopy litterfall along a precipitation and soil fertility gradient in a Panamanian lower montane forest. Biotropica 47: 300–309. https://doi.org/10.1111/btp.12214 Holdridge L.R. (1978) Ecología basada en zonas de vida. San José, IICA. Hoshino D., Nishimura N., Yamamoto S. (2001) Age, size structure and spatial pattern of major tree species in an old-growth Chamaecyparis obtusa forest, Central Japan. Forest Ecology and Management 152: 31–43. https://doi.org/10.1016/S0378- 1127(00)00614-9 IUCN (2017) The IUCN Red List of Threatened Species. Version 2017-1. Available from http://www.iucnredlist.org [accessed 12 Dec. 2016]. Jaramillo D. (2002) Introducción a la ciencia del suelo. Medellín, Leo Digital Jordan C.F. (1985) Nutrient cycling in tropical forest ecosystems. Chichester, Wiley Krings M., Taylor T.N., Dotzler N. (2013) Fossil evidence of the zygomycetous fungi. Persoonia 30: 1–10. https://doi. org/10.3767/003158513X664819 Kwon J.-H., Kang S.-W., Kim J.-S., Park C.-S. (2001) Rhizopus soft rot on cherry tomato caused by Rhizopus stolonifer in Korea. Mycobiology 29: 176–178. https://doi.org/10.1080/122980 93.2001.12015783 López D.M., Bock B.C., Bedoya G. (2008) Genetic structure in remnant populations of an endangered Andean Magnolia. Biotropica 40: 375–379. Mandyam K., Jumpponen A. (2005) Seeking the elusive function of the root-colonising dark septate endophytic fungi. Studies in Mycology 53: 173–189. https://doi.org/10.3114/sim.53.1.173 Newsham K.K., Fitter A.H., Watkinson A.R. (1995) Multi-functionality and biodiversity in arbuscular mycorrhizas. Trends in Ecology & Evolution 10: 407–411. https://doi.org/10.1016/ S0169-5347(00)89157-0 Oehl F., Sieverding E., Palenzuela J., Ineichen K., Alves da Silva G. (2011) Advances in Glomeromycota taxonomy and classification. IMA Fungus 2: 191–199. https://doi.org/10.5598/imafungus. 2011.02.02.10 Osorio N.W. (2014) Manejo de nutrientes en suelos del trópico. Medellín, Vieco SAS Osorio W., Ruiz O. (2013) Guía para la toma de muestras. Laboratorio de Suelos. Medellín, Universidad Nacional de Colombia sede Medellín. Otero J.T., Flanagan N.S., Allen Herre E., Ackerman J.D., Bayman P. (2007) Mycorrhizal function in the neotropical, epiphytic orchid Ionopsis utricularioides (Orchidaceae). American Journal of Botany 94: 1944–1950. https://doi.org/10.3732/ ajb.94.12.1944 Pellissier L., Pinto-Figueroa E., Niculita-Hirzel H., Moora M., Villard L., Goudet J., Guex N., Pagni M., Xenarios I., Sanders I.,Guisan A. (2013) Plant species distributions along environmental gradients: do belowground interactions with fungi matter? Frontiers in Plant Science 4: 1–9. https://doi.org/10.3389/ fpls.2013.00500 Perry E., Hickman G. (1999) A survey to determine the baseline nitrogen leaf concentration of twenty-five landscape tree species. Slosson Project Report 1999–2001. Available from http://slosson. ucdavis.edu/newsletters/Perry_200029041.pdf [accessed 14 Dec. 2018]. Peterson R.L., Wagg C., Pautler M. (2008) Associations between microfungal endophytes and roots: do structural features indicate function? Botany 86: 445–456. https://doi.org/10.1139/ B08-016 Pitman N.C.A., Jørgensen P.M. (2002) Estimating the size of the world’s threatened flora. Science 298: 989. https://doi. org/10.1126/science.298.5595.989 Primack R., Rozzi R., Feinsinger P., Dirzo R., Massardo F. (2001) Fundamentos de conservación biológica: perspectivas latinoamericanas. México D.F., Fondo de Cultura Económica Reinoso Y., Vaillant D., Casadesús L., García E., Pazos V. (2007) Cepas de Brevibacillus laterosporus y Brevibacillus brevis antagonistas de bacterias y hongos fitopatógenos del Cultivo de la Papa (Solanum Tuberosum L.). Fitosanidad 11: 79–80. Rivers M., Beech E., Murphy L., Oldfield S. (2016) The Red List of Magnoliaceae revised and extended. Richmond, Botanic Gardens Conservation International. Available from https://www. bgci.org/files/Global_Trees_Campaign/Magnolia/Magnoliaceae_ RedList2016_LowRes.pdf [accessed 14 Dec. 2018]. Rodriguez R.J., Henson J., Van Volkenburgh E., Hoy M., Wright L., Beckwith F., Kim Y.-O., Redman R.S. (2008) Stress tolerance in plants via habitat-adapted symbiosis. The ISME Journal 2: 404–416. https://doi.org/10.1038/ismej.2007.106 Rodriguez R., Redman R. (2008) More than 400 million years of evolution and some plants still can’t make it on their own: plant stress tolerance via fungal symbiosis. Journal of experimental botany 59: 1109–1114. https://doi.org/10.1093/jxb/erm342 Rodriguez R.J., Freeman D.C., Durant Mcarthur E., Kim Y.O., Redman R.S. (2009) Symbiotic regulation of plant growth, development and reproduction. Communicative & Integrative Biology 2: 141–143. R Studio Team (2015) RStudio: integrated development for R. Boston, RStudio, Inc. Available from http://www.rstudio.com/ [accessed 10 Dec. 2015]. Ruiu L. (2013) Brevibacillus laterosporus, a pathogen of invertebrates and a broad-spectrum antimicrobial species. Insects 4: 476–492. https://doi.org/10.3390/insects4030476 Sánchez de Prager M., Posada R., Velásquez D. Narváez M. (2010) Metodologías básicas para el trabajo con micorriza arbuscular y hongos formadores de micorriza arbuscular. Palmira, Universidad Nacional de Colombia sede Palmira. Sánchez-Velásquez L.R., Pineda-López M.D.R. (2009) Comparative demographic analysis in contrasting environments of Magnolia dealbata: an endangered species from Mexico. Population Ecology 52: 203–210. https://doi.org/10.1007/s10144- 009-0161-5 Serna M., Velásquez C. (2003) Implementación de una estrategia de conservación de las especies de Magnoliaceae en la jurisdicción de CORANTIOQUIA Fase II. Medellín, CORANTIOQUIA– Jardín Botánico de Medellín. Serna M., Velásquez C., Cogollo A. (2009) Novedades taxonómicas y un nuevo registro de Magnoliaceae para Colombia. Brittonia 61: 35–40. https://doi.org/10.1007/s12228-008-9055-7 Siqueira J.O., Carneiro M.A.C., Curi N., Rosado S.C.S., Davide A.C. (1998) Mycorrhizal colonization and mycotrophic growth of native woody species as related to successional groups in Southeastern Brazil. Forest Ecology and Management 107: 241–252. https://doi.org/10.1016/S0378-1127(97)00336-8 Smith S.E., Read D. (2008) Mycorrhizal symbiosis. 3rd Ed. London, Academic Press. https://doi.org/10.1016/B978-0-12- 370526-6.X5001-6 Soil Science Division Staff (2017) Examination and description of soil profiles In: Ditzler C., Scheffe K., Monger H.C. (eds) Soil survey manual. USDA Handbook 18. Washington, Government Printing Office. Available from https://www.nrcs.usda.gov/wps/ portal/nrcs/detail/soils/ref/?cid=nrcs142p2_054262 [accessed 23 May 2017]. Swarts N.D., Dixon K.W. (2009) Terrestrial orchid conservation in the age of extinction. Annals of Botany 104: 543–556. https:// doi.org/10.1093/aob/mcp025 ter Braak C.J.F., Šmilauer P. (2009) CANOCO for Windows. Version 4.56. Wageningen, Biometrics-Plant Research International. Vierheilig H., Piché Y. (1998) A modified procedure for staining arbuscular mycorrhizal fungi in roots. Zeitschrift für Pflanzenernährung und Bodenkunde 161: 601–602. https://doi. org/10.1002/jpln.1998.3581610515 Vinogradov A.E. (2003) Selfish DNA is maladaptive: evidence from the plant Red List. Trends in Genetics 19: 609–614. https:// doi.org/10.1016/j.tig.2003.09.010 Whipps J., Lumsden R. (1989) Biotechnology of fungi for improving plant growth: symposium of the British Mycological Society. Cambridge, NY, Cambridge University Press Wu D., Chen Z. (2000) Effects of human disturbance on the population dynamics of Manglietia fordiana Oliv. In: Liu Y.H., Fan H.M., Chen Z.Y., Wu Q.G., Zen Q.W. (eds) Proceedings of the International Symposium on the family Magnoliaceae, May 18–22, 1998, Guangzhou, China: 65–74. Beijing, Science Press. Yang A., Lu L., Wu C., Xia M. (2011) Arbuscular mycorrhizal fungi associated with Huangshan Magnolia (Magnolia cylindrica). Journal of Medicinal Plants Research 5: 4542–4548. Yepes M. (2007) Evaluación de las poblaciones del guanábano de monte (Magnolia silvioi) en el área de reserva del distrito de manejo integrado del cañón del río Alicante. Medellín, CORANTIOQUIA. Yepes-Quintero A., Duque-Montoya A.J., Navarrete-Encinales D., Phillips-Bernal J., Cabrera-Montenegro E., Corrales-Osorio A., Álvarez-Dávila E., Galindo-García G., García-Dávila M.C., Idárraga A., Vargas-Galvis D. (2011) Estimación de las reservas y pérdidas de carbono por deforestación en los bosques del departamento de Antioquia, Colombia. Actualidades Biológicas 33(95): 193–208. Zangaro W., Nisizaki S., Domingos J., Nakano E. (2003) Mycorrhizal response and successional status in 80 woody species from south Brazil. Journal of Tropical Ecology 19: 315–324. https:// doi.org/10.1017/S0266467403003341 |
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Serna González, Llinet Marcelaffef650f-7c6e-4e37-bff0-f7f58126054dUrrego Giraldo, Ligia Estela76a9f8b1-989d-481d-acbb-9ac816389914Osorio Vega, Nelson Walterc19eded3-8b61-42ee-9ecd-91f1552a9affValencia Ríos, Diegocee87506-e907-4f9b-809b-26c2602d5b032023-04-26T00:04:47Z2023-04-26T00:04:47Z20192032-3913https://dspace.tdea.edu.co/handle/tdea/28252032-3921Background and aims – Magnolia species are highly endangered in neotropical forests where they are highly endemic and often very rare. However, little is known about their nutritional and soil conditions in natural forests. In this study, we focused on two endangered Magnolia species that cohabit in the Colombian Andean cloud forests in order to identify their conservation and nutritional status. We hypothesize that these species might exhibit mycorrhizal colonization that enhance nutrients uptake in poor and disturbed soils. Methods – Individuals of Magnolia jardinensis and M. yarumalensis were assessed in 11 000 m2 of Andean forests remnants from Jardín municipality (Antioquia, Colombia). Foliar and soil samples were analysed in the lab. Through a Principal Component Analysis (PCA) we identified the relationship between soil conditions and foliar nutrition. Root fragments and rhizosphere samples from seedlings and juveniles up to 3 m tall were collected to verify mycorrhizal colonization and presence of other microorganisms. Adults were excluded of the sampling due to the difficulties to differentiate their roots among the rest of the species in the forest fragments. Key results – The surveys show that the M. yarumalensis population has an inverted J-shaped diametric distribution suggesting a potential recovering population while the smaller overall distribution of M. jardinensis in all diametric categories suggests that this species is likely to become extinct. Both species grow in acidic, infertile soils, although foliar nutrient concentrations did not correlate with soil-nutrient availability. Such a discrepancy and the high colonization levels of mycorrhizae (60–70%) and dark septate endophytes (40–45%), suggest that plant-microorganisms may facilitate nutrition and enhance survival of Magnolia species in stressed environments. Other fungi and bacteria were also found in their rhizosphere, but their role with respect to Magnolia species remains unclear. Conclusions - Mycorrhizal colonization of endangered Magnolia species seems to play a key role to their performance in natural disturbed Andean forests. Aspects related to soil and rhizosphere ecology should be included in conservation projects for endangered and endemic plants. Key words – Rhizosphere, Andean cloud forests, Magnolia jardinensis, Magnolia yarumalensis, soil fertility, tree nutrition, conservation, mycorrhizae.11 páginasapplication/pdfengNational Botanic Garden of Belgium : Royal Botanical Society of BelgiumBélgicahttps://creativecommons.org/licenses/by/4.0/Atribución 4.0 Internacional (CC BY 4.0)info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2DOI:10.1088/2053-1591/ab4316ConservationConservaçãoConservaciónRhizosphereRizosferaRizosferaSoil fertilityFertilidade do soloFertilidad del sueloMycorrhizaeAndean cloud forestsMagnolia jardinensisMagnolia yarumalensisTree nutritionMycorrhizae: a key interaction for conservation of two endangered Magnolias from Andean forestsArtículo de revistahttp://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85Jardín, Antioquia, Colombia40130152Plant ecology and evolutionAlarcón A.L. (2001) El boro como nutriente esencial. Revista Horticultura 155: 1–11.Alemañy-Merly S.E. (1999) Magnolia portoricensis Bello. New Orleans, Department of Agriculture, Forest Service, Southern Forest Experiment StationAndersen K.M., Turner B.L., Dalling J.W. (2010) Soil-based habitat partitioning in understorey palms in lower montane tropical forests. Journal of Biogeography 37: 278–292. https://doi. org/10.1111/j.1365-2699.2009.02192.xArmenteras D., Rodríguez N., Retana J., Morales M. (2011) Understanding deforestation in montane and lowland forests of the Colombian Andes. Regional Environmental Change 11: 693– 705. https://doi.org/10.1007/s10113-010-0200-yBarrow J.R., Osuna P. (2002) Phosphorus solubilization and uptake by dark septate fungi in fourwing saltbush, Atriplex canescens (Pursh) Nutt. Journal of Arid Environments 51: 449–459. https:// doi.org/10.1006/jare.2001.0925Becker P., Castillo A. (1990) Root architecture of shrubs and saplings in the understory of a tropical moist forest in lowland Panama. Biotropica 22: 242–249. https://doi.org/10.2307/2388534Bleish L., Xie J. (1998) Selected nutrient analysis of plants in the diet of the Guizhou snub-nosed monkey (Rhinopithecus brelkichi). In: Jablonski N (ed.) The natural history of the doucs and snub-nosed monkeys: 252. London, World ScientificBlinn C., Bucker E. (1989) Normal foliar nutrient levels in North American forest trees Station Bulletin 590-1989 (Item No. ADSB- 3762). St Paul, University of Minnesota.Bruijnzeel L.A. (1991) Nutrient input-output budgets of tropical forest ecosystems: a review. Journal of Tropical Ecology 7: 1–24. https://doi.org/10.1017/S0266467400005010Cámara-Leret R., Tuomisto H., Ruokolainen K., Balslev H., Munch Kristiansen S. (2017) Modelling responses of western Amazonian palms to soil nutrients. Journal of Ecology 105: 367–381. https://doi.org/10.1111/1365-2745.12708Cano M.A. (2011) Interacción de microorganismos benéficos en plantas: Micorrizas, Trichoderma spp. y Pseudomonas spp. Una revisión. Revista U.D.C.A. Actualidad & Divulgación Científica 14: 15–31.Castro F. (1996) Base técnica para el conocimiento y manejo del suelo del Valle del Alto Magdalena. Espinal, CORPOICA.Chen H., Gurmesa G.A., Liu L., Zhang T., Fu S., Liu Z., Zhanfeng L., Shaofeng D., Chuan M., Jianming M. (2014) Effects of litter manipulation on litter decomposition in a successional gradients of tropical forests in southern China. PLOS ONE 9: e99018. https://doi.org/10.1371/journal.pone.0099018Clark D. (2002) Los factores edáficos y la distribución de las plantas. In: Guariguatta M., Kattan G. (eds) Ecología y conservación de bosques neotropicales: 193–222. Cartago, Ediciones LUR.CORANTIOQUIA (2011) Avances en la estrategia para la conservación de la familia Magnoliaceae en jurisdicción de CORANTIOQUIA. Boletín Técnico Biodiversidad 6: 100–101.Dalpé Y., Séguin S.M. (2013) Microwave-assisted technology for the clearing and staining of arbuscular mycorrhizal fungi in roots. Mycorrhiza 23: 333–340. https://doi.org/10.1007/s00572- 012-0472-9de Oliveira Freitas R., Buscardo E., Nagy L., dos Santos Maciel A.B., Carrenho R. Luizão R.C.C. (2014) Arbuscular mycorrhizal fungal communities along a pedo-hydrological gradient in a Central Amazonian terra firme forest. Mycorrhiza 24: 21–32. https://doi.org/10.1007/s00572-013-0507-xde Souza R., Ambrosini A., Passaglia L.M.P. (2015) Plant growthpromoting bacteria as inoculants in agricultural soils. Genetics and Molecular Biology 38: 401–419. https://doi.org/10.1590/ S1415-475738420150053Espinoza L., Slaton N., Mozaffari M. (2000) Como interpretar los resultados de los análisis de suelos. Agricultura y Recursos Naturales FSA2118SP. Little Rock, University of Arkansas.Etter A., McAlpine C., Wilson K., Phinn S., Possingham H. (2006) Regional patterns of agricultural land use and deforestation in Colombia. Agriculture, Ecosystems & Environment 114: 369– 386. https://doi.org/10.1016/j.agee.2005.11.013Fitter A.H., Helgason T., Hodge A. (2011) Nutritional exchanges in the arbuscular mycorrhizal symbiosis: implications for sustainable agriculture. Fungal Biology Reviews 25: 68–72. https:// doi.org/10.1016/j.fbr.2011.01.002Garcés de Granada E., Martha O.D.A., Bautista G.R., Valencia H. (2001) Fusarium oxysporum el hongo que nos falta conocer. Acta Biológica Colombiana 6: 7–26.Giovannetti M., Mosse B. (1980) An evaluation of techniques for measuring vesicular arbuscular mycorrhizal infection in roots. New Phytologist 84: 489–500. https://doi. org/10.1111/j.1469-8137.1980.tb04556.xGirmé G., Grau E., Calvo M.A., Leonardo E. (2014) Clave dicotómica para la identificación de hongos aislados sistemáticamente en ambientes mediterráneos. Revista de La Sociedad Española de Microbiología 57: 69–71.Grilli G., Urcelay C., Galetto L. (2013) Linking mycorrhizal fungi and soil nutrients to vegetative and reproductive ruderal plant development in a fragmented forest at central Argentina. Forest Ecology and Management 310: 442–449. https://doi. org/10.1016/j.foreco.2013.08.052Gutierrez L., Vovides A.P. (1997) An in situ study of Magnolia dealbata Zucc. in Veracruz State: an endangered endemic tree of Mexico. Biodiversity & Conservation 6: 89–97. http://doi. org/10.1023/A%3A1018327700030Heineman K.D., Caballero P., Morris A., Velasquez C., Serrano K., Ramos N., Gonzalez J., Mayorga L., Corre M.D., Dalling J.W. (2015) Variation in canopy litterfall along a precipitation and soil fertility gradient in a Panamanian lower montane forest. Biotropica 47: 300–309. https://doi.org/10.1111/btp.12214Holdridge L.R. (1978) Ecología basada en zonas de vida. San José, IICA.Hoshino D., Nishimura N., Yamamoto S. (2001) Age, size structure and spatial pattern of major tree species in an old-growth Chamaecyparis obtusa forest, Central Japan. Forest Ecology and Management 152: 31–43. https://doi.org/10.1016/S0378- 1127(00)00614-9IUCN (2017) The IUCN Red List of Threatened Species. Version 2017-1. Available from http://www.iucnredlist.org [accessed 12 Dec. 2016].Jaramillo D. (2002) Introducción a la ciencia del suelo. Medellín, Leo DigitalJordan C.F. (1985) Nutrient cycling in tropical forest ecosystems. Chichester, WileyKrings M., Taylor T.N., Dotzler N. (2013) Fossil evidence of the zygomycetous fungi. Persoonia 30: 1–10. https://doi. org/10.3767/003158513X664819Kwon J.-H., Kang S.-W., Kim J.-S., Park C.-S. (2001) Rhizopus soft rot on cherry tomato caused by Rhizopus stolonifer in Korea. Mycobiology 29: 176–178. https://doi.org/10.1080/122980 93.2001.12015783López D.M., Bock B.C., Bedoya G. (2008) Genetic structure in remnant populations of an endangered Andean Magnolia. Biotropica 40: 375–379.Mandyam K., Jumpponen A. (2005) Seeking the elusive function of the root-colonising dark septate endophytic fungi. Studies in Mycology 53: 173–189. https://doi.org/10.3114/sim.53.1.173Newsham K.K., Fitter A.H., Watkinson A.R. (1995) Multi-functionality and biodiversity in arbuscular mycorrhizas. Trends in Ecology & Evolution 10: 407–411. https://doi.org/10.1016/ S0169-5347(00)89157-0Oehl F., Sieverding E., Palenzuela J., Ineichen K., Alves da Silva G. (2011) Advances in Glomeromycota taxonomy and classification. IMA Fungus 2: 191–199. https://doi.org/10.5598/imafungus. 2011.02.02.10Osorio N.W. (2014) Manejo de nutrientes en suelos del trópico. Medellín, Vieco SASOsorio W., Ruiz O. (2013) Guía para la toma de muestras. Laboratorio de Suelos. Medellín, Universidad Nacional de Colombia sede Medellín.Otero J.T., Flanagan N.S., Allen Herre E., Ackerman J.D., Bayman P. (2007) Mycorrhizal function in the neotropical, epiphytic orchid Ionopsis utricularioides (Orchidaceae). American Journal of Botany 94: 1944–1950. https://doi.org/10.3732/ ajb.94.12.1944Pellissier L., Pinto-Figueroa E., Niculita-Hirzel H., Moora M., Villard L., Goudet J., Guex N., Pagni M., Xenarios I., Sanders I.,Guisan A. (2013) Plant species distributions along environmental gradients: do belowground interactions with fungi matter? Frontiers in Plant Science 4: 1–9. https://doi.org/10.3389/ fpls.2013.00500Perry E., Hickman G. (1999) A survey to determine the baseline nitrogen leaf concentration of twenty-five landscape tree species. Slosson Project Report 1999–2001. Available from http://slosson. ucdavis.edu/newsletters/Perry_200029041.pdf [accessed 14 Dec. 2018].Peterson R.L., Wagg C., Pautler M. (2008) Associations between microfungal endophytes and roots: do structural features indicate function? Botany 86: 445–456. https://doi.org/10.1139/ B08-016Pitman N.C.A., Jørgensen P.M. (2002) Estimating the size of the world’s threatened flora. Science 298: 989. https://doi. org/10.1126/science.298.5595.989Primack R., Rozzi R., Feinsinger P., Dirzo R., Massardo F. (2001) Fundamentos de conservación biológica: perspectivas latinoamericanas. México D.F., Fondo de Cultura EconómicaReinoso Y., Vaillant D., Casadesús L., García E., Pazos V. (2007) Cepas de Brevibacillus laterosporus y Brevibacillus brevis antagonistas de bacterias y hongos fitopatógenos del Cultivo de la Papa (Solanum Tuberosum L.). Fitosanidad 11: 79–80.Rivers M., Beech E., Murphy L., Oldfield S. (2016) The Red List of Magnoliaceae revised and extended. Richmond, Botanic Gardens Conservation International. Available from https://www. bgci.org/files/Global_Trees_Campaign/Magnolia/Magnoliaceae_ RedList2016_LowRes.pdf [accessed 14 Dec. 2018].Rodriguez R.J., Henson J., Van Volkenburgh E., Hoy M., Wright L., Beckwith F., Kim Y.-O., Redman R.S. (2008) Stress tolerance in plants via habitat-adapted symbiosis. The ISME Journal 2: 404–416. https://doi.org/10.1038/ismej.2007.106Rodriguez R., Redman R. (2008) More than 400 million years of evolution and some plants still can’t make it on their own: plant stress tolerance via fungal symbiosis. Journal of experimental botany 59: 1109–1114. https://doi.org/10.1093/jxb/erm342Rodriguez R.J., Freeman D.C., Durant Mcarthur E., Kim Y.O., Redman R.S. (2009) Symbiotic regulation of plant growth, development and reproduction. Communicative & Integrative Biology 2: 141–143.R Studio Team (2015) RStudio: integrated development for R. Boston, RStudio, Inc. Available from http://www.rstudio.com/ [accessed 10 Dec. 2015].Ruiu L. (2013) Brevibacillus laterosporus, a pathogen of invertebrates and a broad-spectrum antimicrobial species. Insects 4: 476–492. https://doi.org/10.3390/insects4030476Sánchez de Prager M., Posada R., Velásquez D. Narváez M. (2010) Metodologías básicas para el trabajo con micorriza arbuscular y hongos formadores de micorriza arbuscular. Palmira, Universidad Nacional de Colombia sede Palmira.Sánchez-Velásquez L.R., Pineda-López M.D.R. (2009) Comparative demographic analysis in contrasting environments of Magnolia dealbata: an endangered species from Mexico. Population Ecology 52: 203–210. https://doi.org/10.1007/s10144- 009-0161-5Serna M., Velásquez C. (2003) Implementación de una estrategia de conservación de las especies de Magnoliaceae en la jurisdicción de CORANTIOQUIA Fase II. Medellín, CORANTIOQUIA– Jardín Botánico de Medellín.Serna M., Velásquez C., Cogollo A. (2009) Novedades taxonómicas y un nuevo registro de Magnoliaceae para Colombia. Brittonia 61: 35–40. https://doi.org/10.1007/s12228-008-9055-7Siqueira J.O., Carneiro M.A.C., Curi N., Rosado S.C.S., Davide A.C. (1998) Mycorrhizal colonization and mycotrophic growth of native woody species as related to successional groups in Southeastern Brazil. Forest Ecology and Management 107: 241–252. https://doi.org/10.1016/S0378-1127(97)00336-8Smith S.E., Read D. (2008) Mycorrhizal symbiosis. 3rd Ed. London, Academic Press. https://doi.org/10.1016/B978-0-12- 370526-6.X5001-6Soil Science Division Staff (2017) Examination and description of soil profiles In: Ditzler C., Scheffe K., Monger H.C. (eds) Soil survey manual. USDA Handbook 18. Washington, Government Printing Office. Available from https://www.nrcs.usda.gov/wps/ portal/nrcs/detail/soils/ref/?cid=nrcs142p2_054262 [accessed 23 May 2017].Swarts N.D., Dixon K.W. (2009) Terrestrial orchid conservation in the age of extinction. Annals of Botany 104: 543–556. https:// doi.org/10.1093/aob/mcp025ter Braak C.J.F., Šmilauer P. (2009) CANOCO for Windows. Version 4.56. Wageningen, Biometrics-Plant Research International.Vierheilig H., Piché Y. (1998) A modified procedure for staining arbuscular mycorrhizal fungi in roots. Zeitschrift für Pflanzenernährung und Bodenkunde 161: 601–602. https://doi. org/10.1002/jpln.1998.3581610515Vinogradov A.E. (2003) Selfish DNA is maladaptive: evidence from the plant Red List. Trends in Genetics 19: 609–614. https:// doi.org/10.1016/j.tig.2003.09.010Whipps J., Lumsden R. (1989) Biotechnology of fungi for improving plant growth: symposium of the British Mycological Society. Cambridge, NY, Cambridge University PressWu D., Chen Z. (2000) Effects of human disturbance on the population dynamics of Manglietia fordiana Oliv. In: Liu Y.H., Fan H.M., Chen Z.Y., Wu Q.G., Zen Q.W. (eds) Proceedings of the International Symposium on the family Magnoliaceae, May 18–22, 1998, Guangzhou, China: 65–74. Beijing, Science Press.Yang A., Lu L., Wu C., Xia M. (2011) Arbuscular mycorrhizal fungi associated with Huangshan Magnolia (Magnolia cylindrica). Journal of Medicinal Plants Research 5: 4542–4548.Yepes M. (2007) Evaluación de las poblaciones del guanábano de monte (Magnolia silvioi) en el área de reserva del distrito de manejo integrado del cañón del río Alicante. Medellín, CORANTIOQUIA.Yepes-Quintero A., Duque-Montoya A.J., Navarrete-Encinales D., Phillips-Bernal J., Cabrera-Montenegro E., Corrales-Osorio A., Álvarez-Dávila E., Galindo-García G., García-Dávila M.C., Idárraga A., Vargas-Galvis D. (2011) Estimación de las reservas y pérdidas de carbono por deforestación en los bosques del departamento de Antioquia, Colombia. Actualidades Biológicas 33(95): 193–208.Zangaro W., Nisizaki S., Domingos J., Nakano E. (2003) Mycorrhizal response and successional status in 80 woody species from south Brazil. Journal of Tropical Ecology 19: 315–324. https:// doi.org/10.1017/S0266467403003341ORIGINALMycorrhizae A key interaction for conservation of two endangered magnolias from Andean forests.pdfMycorrhizae A key interaction for conservation of two endangered magnolias from Andean forests.pdfapplication/pdf1903989https://dspace.tdea.edu.co/bitstream/tdea/2825/1/Mycorrhizae%20A%20key%20interaction%20for%20conservation%20of%20two%20endangered%20magnolias%20from%20Andean%20forests.pdf05f136e11555a3f7ecb581deb3bb7125MD51open accessLICENSElicense.txtlicense.txttext/plain; charset=utf-814828https://dspace.tdea.edu.co/bitstream/tdea/2825/2/license.txt2f9959eaf5b71fae44bbf9ec84150c7aMD52open accessTEXTMycorrhizae A key interaction for conservation of two endangered magnolias from Andean forests.pdf.txtMycorrhizae A key interaction for conservation of two endangered magnolias from Andean forests.pdf.txtExtracted 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 incorporada en las Obras Colectivas.

b.	Distribuir copias o fonogramas de las Obras, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública, incluyéndolas como incorporadas en Obras Colectivas, según corresponda.

c.	Distribuir copias de las Obras Derivadas que se generen, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública.
Los derechos mencionados anteriormente pueden ser ejercidos en todos los medios y formatos, actualmente conocidos o que se inventen en el futuro. Los derechos antes mencionados incluyen el derecho a realizar dichas modificaciones en la medida que sean técnicamente necesarias para ejercer los derechos en otro medio o formatos, pero de otra manera usted no está autorizado para realizar obras derivadas. Todos los derechos no otorgados expresamente por el Licenciante quedan por este medio reservados, incluyendo pero sin limitarse a aquellos que se mencionan en las secciones 4(d) y 4(e).

4. Restricciones.
La licencia otorgada en la anterior Sección 3 está expresamente sujeta y limitada por las siguientes restricciones:

a.	Usted puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra sólo bajo las condiciones de esta Licencia, y Usted debe incluir una copia de esta licencia o del Identificador Universal de Recursos de la misma con cada copia de la Obra que distribuya, exhiba públicamente, ejecute públicamente o ponga a disposición pública. No es posible ofrecer o imponer ninguna condición sobre la Obra que altere o limite las condiciones de esta Licencia o el ejercicio de los derechos de los destinatarios otorgados en este documento. No es posible sublicenciar la Obra. Usted debe mantener intactos todos los avisos que hagan referencia a esta Licencia y a la cláusula de limitación de garantías. Usted no puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra con alguna medida tecnológica que controle el acceso o la utilización de ella de una forma que sea inconsistente con las condiciones de esta Licencia. Lo anterior se aplica a la Obra incorporada a una Obra Colectiva, pero esto no exige que la Obra Colectiva aparte de la obra misma quede sujeta a las condiciones de esta Licencia. Si Usted crea una Obra Colectiva, previo aviso de cualquier Licenciante debe, en la medida de lo posible, eliminar de la Obra Colectiva cualquier referencia a dicho Licenciante o al Autor Original, según lo solicitado por el Licenciante y conforme lo exige la cláusula 4(c).

b.	Usted no puede ejercer ninguno de los derechos que le han sido otorgados en la Sección 3 precedente de modo que estén principalmente destinados o directamente dirigidos a conseguir un provecho comercial o una compensación monetaria privada. El intercambio de la Obra por otras obras protegidas por derechos de autor, ya sea a través de un sistema para compartir archivos digitales (digital file-sharing) o de cualquier otra manera no será considerado como estar destinado principalmente o dirigido directamente a conseguir un provecho comercial o una compensación monetaria privada, siempre que no se realice un pago mediante una compensación monetaria en relación con el intercambio de obras protegidas por el derecho de autor.

c.	Si usted distribuye, exhibe públicamente, ejecuta públicamente o ejecuta públicamente en forma digital la Obra o cualquier Obra Derivada u Obra Colectiva, Usted debe mantener intacta toda la información de derecho de autor de la Obra y proporcionar, de forma razonable según el medio o manera que Usted esté utilizando: (i) el nombre del Autor Original si está provisto (o seudónimo, si fuere aplicable), y/o (ii) el nombre de la parte o las partes que el Autor Original y/o el Licenciante hubieren designado para la atribución (v.g., un instituto patrocinador, editorial, publicación) en la información de los derechos de autor del Licenciante, términos de servicios o de otras formas razonables; el título de la Obra si está provisto; en la medida de lo razonablemente factible y, si está provisto, el Identificador Uniforme de Recursos (Uniform Resource Identifier) que el Licenciante especifica para ser asociado con la Obra, salvo que tal URI no se refiera a la nota sobre los derechos de autor o a la información sobre el licenciamiento de la Obra; y en el caso de una Obra Derivada, atribuir el crédito identificando el uso de la Obra en la Obra Derivada (v.g., "Traducción Francesa de la Obra del Autor Original," o "Guión Cinematográfico basado en la Obra original del Autor Original"). Tal crédito puede ser implementado de cualquier forma razonable; en el caso, sin embargo, de Obras Derivadas u Obras Colectivas, tal crédito aparecerá, como mínimo, donde aparece el crédito de cualquier otro autor comparable y de una manera, al menos, tan destacada como el crédito de otro autor comparable.

d.	Para evitar toda confusión, el Licenciante aclara que, cuando la obra es una composición musical:

i.	Regalías por interpretación y ejecución bajo licencias generales. El Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública o la ejecución pública digital de la obra y de recolectar, sea individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, SAYCO), las regalías por la ejecución pública o por la ejecución pública digital de la obra (por ejemplo Webcast) licenciada bajo licencias generales, si la interpretación o ejecución de la obra está primordialmente orientada por o dirigida a la obtención de una ventaja comercial o una compensación monetaria privada.

ii.	Regalías por Fonogramas. El Licenciante se reserva el derecho exclusivo de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, los consagrados por la SAYCO), una agencia de derechos musicales o algún agente designado, las regalías por cualquier fonograma que Usted cree a partir de la obra (“versión cover”) y distribuya, en los términos del régimen de derechos de autor, si la creación o distribución de esa versión cover está primordialmente destinada o dirigida a obtener una ventaja comercial o una compensación monetaria privada.

e.	Gestión de Derechos de Autor sobre Interpretaciones y Ejecuciones Digitales (WebCasting). Para evitar toda confusión, el Licenciante aclara que, cuando la obra sea un fonograma, el Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública digital de la obra (por ejemplo, webcast) y de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, ACINPRO), las regalías por la ejecución pública digital de la obra (por ejemplo, webcast), sujeta a las disposiciones aplicables del régimen de Derecho de Autor, si esta ejecución pública digital está primordialmente dirigida a obtener una ventaja comercial o una compensación monetaria privada.

5. Representaciones, Garantías y Limitaciones de Responsabilidad.
A MENOS QUE LAS PARTES LO ACORDARAN DE OTRA FORMA POR ESCRITO, EL LICENCIANTE OFRECE LA OBRA (EN EL ESTADO EN EL QUE SE ENCUENTRA) “TAL CUAL”, SIN BRINDAR GARANTÍAS DE CLASE ALGUNA RESPECTO DE LA OBRA, YA SEA EXPRESA, IMPLÍCITA, LEGAL O CUALQUIERA OTRA, INCLUYENDO, SIN LIMITARSE A ELLAS, GARANTÍAS DE TITULARIDAD, COMERCIABILIDAD, ADAPTABILIDAD O ADECUACIÓN A PROPÓSITO DETERMINADO, AUSENCIA DE INFRACCIÓN, DE AUSENCIA DE DEFECTOS LATENTES O DE OTRO TIPO, O LA PRESENCIA O AUSENCIA DE ERRORES, SEAN O NO DESCUBRIBLES (PUEDAN O NO SER ESTOS DESCUBIERTOS). ALGUNAS JURISDICCIONES NO PERMITEN LA EXCLUSIÓN DE GARANTÍAS IMPLÍCITAS, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.

6. Limitación de responsabilidad.
A MENOS QUE LO EXIJA EXPRESAMENTE LA LEY APLICABLE, EL LICENCIANTE NO SERÁ RESPONSABLE ANTE USTED POR DAÑO ALGUNO, SEA POR RESPONSABILIDAD EXTRACONTRACTUAL, PRECONTRACTUAL O CONTRACTUAL, OBJETIVA O SUBJETIVA, SE TRATE DE DAÑOS MORALES O PATRIMONIALES, DIRECTOS O INDIRECTOS, PREVISTOS O IMPREVISTOS PRODUCIDOS POR EL USO DE ESTA LICENCIA O DE LA OBRA, AUN CUANDO EL LICENCIANTE HAYA SIDO ADVERTIDO DE LA POSIBILIDAD DE DICHOS DAÑOS. ALGUNAS LEYES NO PERMITEN LA EXCLUSIÓN DE CIERTA RESPONSABILIDAD, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.

7. Término.

a.	Esta Licencia y los derechos otorgados en virtud de ella terminarán automáticamente si Usted infringe alguna condición establecida en ella. Sin embargo, los individuos o entidades que han recibido Obras Derivadas o Colectivas de Usted de conformidad con esta Licencia, no verán terminadas sus licencias, siempre que estos individuos o entidades sigan cumpliendo íntegramente las condiciones de estas licencias. Las Secciones 1, 2, 5, 6, 7, y 8 subsistirán a cualquier terminación de esta Licencia.

b.	Sujeta a las condiciones y términos anteriores, la licencia otorgada aquí es perpetua (durante el período de vigencia de los derechos de autor de la obra). No obstante lo anterior, el Licenciante se reserva el derecho a publicar y/o estrenar la Obra bajo condiciones de licencia diferentes o a dejar de distribuirla en los términos de esta Licencia en cualquier momento; en el entendido, sin embargo, que esa elección no servirá para revocar esta licencia o que deba ser otorgada , bajo los términos de esta licencia), y esta licencia continuará en pleno vigor y efecto a menos que sea terminada como se expresa atrás. La Licencia revocada continuará siendo plenamente vigente y efectiva si no se le da término en las condiciones indicadas anteriormente.

8. Varios.

a.	Cada vez que Usted distribuya o ponga a disposición pública la Obra o una Obra Colectiva, el Licenciante ofrecerá al destinatario una licencia en los mismos términos y condiciones que la licencia otorgada a Usted bajo esta Licencia.

b.	Si alguna disposición de esta Licencia resulta invalidada o no exigible, según la legislación vigente, esto no afectará ni la validez ni la aplicabilidad del resto de condiciones de esta Licencia y, sin acción adicional por parte de los sujetos de este acuerdo, aquélla se entenderá reformada lo mínimo necesario para hacer que dicha disposición sea válida y exigible.

c.	Ningún término o disposición de esta Licencia se estimará renunciada y ninguna violación de ella será consentida a menos que esa renuncia o consentimiento sea otorgado por escrito y firmado por la parte que renuncie o consienta.

d.	Esta Licencia refleja el acuerdo pleno entre las partes respecto a la Obra aquí licenciada. No hay arreglos, acuerdos o declaraciones respecto a la Obra que no estén especificados en este documento. El Licenciante no se verá limitado por ninguna disposición adicional que pueda surgir en alguna comunicación emanada de Usted. Esta Licencia no puede ser modificada sin el consentimiento mutuo por escrito del Licenciante y Usted.
 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