Efficacy of an ultrasound technique in the recovery of spermatozoa during the investigation of sexual offenses

Objective: To evaluate the efficiency for the recovery of spermatozoa using the standard technique and a technique based on ultrasound. Materials and methods: An experimental study was carried out to compare two techniques for spermatozoa recovery; (i) the standard technique, based on elution of the evidence in distilled water with a buffer and, (ii) a modified technique that included concentration by centrifugation and ultrasound. The efficiency was based on manual counting of spermatozoa using dilutions 1:1 to 1:500. Results: The modified technique substantially improved the recovery of spermatozoa. While the standard technique recovered 3.1%, the modified one resulted in a recovery of 17.5% using the 1:10 dilution, which represents a six-fold increase. The modified technique was the only one able to recover spermatozoa in dilutions higher than 1:250. Conclusion: The implementation of the ultrasonic technique improves the recovery of spermatozoa.

• Autores: Lezcano Martínez, Ana Nataly; Giraldo Vásquez, Luz Eliana; Vanegas Londoño, Estefanía; Espinosa Montoya, Tatiana; Zuluaga Barrios, José Daniel; Molina Castaño, Carlos Federico
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2019
• Institución: Tecnológico de Antioquia
• Repositorio: Repositorio Tdea
• Idioma: eng
• OAI Identifier: oai:dspace.tdea.edu.co:tdea/2863
• Acceso en línea: https://dspace.tdea.edu.co/handle/tdea/2863
• Palabra clave: Ultrasound; Ultra-som; Ultrasonido; Spermatozoa; Espermatozoides; Spermatozoïdes; Forensic Genetics; Genética Forense; Génétique légale; Sex offences; Delitos sexuales
• Rights: closedAccess
• License: http://purl.org/coar/access_right/c_14cb