Bioprecipitación de carbonato de calcio mediada por aislados nativos de Bacillus spp

En el ambiente existen diferentes carbonatos inorgánicos en forma de minerales y rocas importantes, entre ellos están la calcita (CaCO3). En cuanto a este compuesto se ha reportado en la literatura que la mayoría de las bacterias heterotróficas del suelo inducen la precipitación de carbonato cálcico...

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Autores:
Zapata Martínez, Juan Felipe
Cardona Echeverry, Andrés
García Ávila, Carolina
Tipo de recurso:
Part of book
Fecha de publicación:
2021
Institución:
Tecnológico de Antioquia
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Repositorio Tdea
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spa
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oai:dspace.tdea.edu.co:tdea/4860
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https://dspace.tdea.edu.co/handle/tdea/4860
Palabra clave:
Ingeniería y operaciones afines
Carbonato de calcio
Bacillus spp
Bioprecipitación
Biomineralización
Curva de crecimiento
Ciencias ambientales e ingeniería
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openAccess
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http://purl.org/coar/access_right/c_abf2
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oai_identifier_str oai:dspace.tdea.edu.co:tdea/4860
network_acronym_str RepoTdea2
network_name_str Repositorio Tdea
repository_id_str
dc.title.none.fl_str_mv Bioprecipitación de carbonato de calcio mediada por aislados nativos de Bacillus spp
title Bioprecipitación de carbonato de calcio mediada por aislados nativos de Bacillus spp
spellingShingle Bioprecipitación de carbonato de calcio mediada por aislados nativos de Bacillus spp
Ingeniería y operaciones afines
Carbonato de calcio
Bacillus spp
Bioprecipitación
Biomineralización
Curva de crecimiento
Ciencias ambientales e ingeniería
title_short Bioprecipitación de carbonato de calcio mediada por aislados nativos de Bacillus spp
title_full Bioprecipitación de carbonato de calcio mediada por aislados nativos de Bacillus spp
title_fullStr Bioprecipitación de carbonato de calcio mediada por aislados nativos de Bacillus spp
title_full_unstemmed Bioprecipitación de carbonato de calcio mediada por aislados nativos de Bacillus spp
title_sort Bioprecipitación de carbonato de calcio mediada por aislados nativos de Bacillus spp
dc.creator.fl_str_mv Zapata Martínez, Juan Felipe
Cardona Echeverry, Andrés
García Ávila, Carolina
dc.contributor.author.none.fl_str_mv Zapata Martínez, Juan Felipe
Cardona Echeverry, Andrés
García Ávila, Carolina
dc.subject.ddc.spa.fl_str_mv Ingeniería y operaciones afines
topic Ingeniería y operaciones afines
Carbonato de calcio
Bacillus spp
Bioprecipitación
Biomineralización
Curva de crecimiento
Ciencias ambientales e ingeniería
dc.subject.proposal.none.fl_str_mv Carbonato de calcio
Bacillus spp
Bioprecipitación
Biomineralización
Curva de crecimiento
dc.subject.unesco.none.fl_str_mv Ciencias ambientales e ingeniería
description En el ambiente existen diferentes carbonatos inorgánicos en forma de minerales y rocas importantes, entre ellos están la calcita (CaCO3). En cuanto a este compuesto se ha reportado en la literatura que la mayoría de las bacterias heterotróficas del suelo inducen la precipitación de carbonato cálcico. Este tipo de investigaciones pueden aportar solución a múltiples problemas ambientales, como lo son la erosión, ecosíntesis, la estabilización de suelos, la fijación de CO2 atmosférico a través de la formación de sedimento de carbonato y litificación, entre otros. El objetivo de este estudio fue establecer las condiciones óptimas en la curva de crecimiento de aislados nativos de Bacillus spp para la producción de cristales, en el cual se encontró que en la fase exponencial de su ciclo de crecimiento es donde los microorganismos poseen mayor capacidad precipitadora, igualmente se determinó que las condiciones óptimas para la bioprecipitación y la estabilidad de los cristales están directamente relacionadas con un pH ligeramente alcalino.
publishDate 2021
dc.date.issued.none.fl_str_mv 2021-12-22
dc.date.accessioned.none.fl_str_mv 2023-12-12T15:15:51Z
dc.date.available.none.fl_str_mv 2023-12-12T15:15:51Z
dc.type.spa.fl_str_mv Capítulo - Parte de Libro
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dc.type.content.spa.fl_str_mv Text
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/bookPart
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/CAP_LIB
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.isbn.spa.fl_str_mv 978-958-8628-69-1
dc.identifier.uri.none.fl_str_mv https://dspace.tdea.edu.co/handle/tdea/4860
dc.identifier.eisbn.spa.fl_str_mv 978-958-8628-70-7
identifier_str_mv 978-958-8628-69-1
978-958-8628-70-7
url https://dspace.tdea.edu.co/handle/tdea/4860
dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.ispartofseries.none.fl_str_mv Investigación;
dc.relation.citationendpage.spa.fl_str_mv 151
dc.relation.citationstartpage.spa.fl_str_mv 135
dc.relation.ispartofbook.spa.fl_str_mv Prácticas y herramientas de sostenibilidad
dc.relation.references.spa.fl_str_mv S. K. Ramachandran, V. Ramakrishnan, and S. S. Bang, «Remediation of Concrete Using Microorganisms,» Acid Materials Journal, vol. 98, no 1, pp. 3-9, 2001, doi: 10.14359/10154.
B. Krajewska, «Urease-aided calcium carbonate mineralization for engineering applications: A review,» Journal of Advanced Research, vol. 13, pp. 59-67, 2018, doi: 10.1016/j.jare.2017.10.009.
S. Weiner, and L. Addadi, «Design strategies in mineralized biological materials,» Juornal Materials Chemistry, vol. 7, no 5, pp. 689-702, ene. 1997, doi: 10.1039/A604512J.
E. E. Rios-Valenciana, R. Briones-Gallardo, L. F. Cházaro-Ruiz, N. Martínez-Villegas, and L. B. Celis, «Role of indigenous microbiota from heavily contaminated sediments in the bioprecipitation of arsenic,» Journal of Hazardous Materials, vol. 339, pp. 114-121, oct. 2017, doi: 10.1016/j.jhazmat.2017.06.019.
C. Lors, D. Damidot, L. Petit, A. Legrix, N. C. Tran, and B. Masson, «Bioprecipitation of a calcium carbonate – Biofilm composite on the surface of concrete for the maintenance of nuclear reactor enclosures,» Construction and Building Materials, vol. 237, p. 117618, 2020, doi: 10.1016/j.conbuildmat.2019.117618.
L. Jiang, X. Liu, H. Yin, Y. Liang, H. Liu, B. Miao, Q. Peng, D. Meng, S. Wang, J. Yang, and Z. Guo, «The utilization of biomineralization technique based on microbial induced phosphate precipitation in remediation of potentially toxic ions contaminated soil: A mini review,» Ecotoxicology and Environmental Safety, vol. 191, p. 110009, 2020, doi: 10.1016/j. ecoenv.2019.110009.
Z. Basaran Bundur, M. J. Kirisits, and R. D. Ferron, «Biomineralized cement-based materials: Impact of inoculating vegetative bacterial cells on hydration and strength,» Cement and Concrete Research, vol. 67, pp. 237-245, 2015, doi: 10.1016/j.cemconres.2014.10.002.
V. Achal, A. Mukherjee, D. Kumari, and Q. Zhang, «Biomineralization for sustainable construction – A review of processes and applications,» Earth-Science Reviews, vol. 148, pp. 1-17, 2015, doi: 10.1016/j.earscirev.2015.05.008.
V. Pasquale, S. Fiore, D. Hlayem, A. Lettino, F. Huertas, E. Chianese, and S. Duomontet, «Biomineralization of carbonates induced by the fungi Paecilomyces inflatus and Plectosphaerella cucumerina,» International Biodeterioration and Biodegradation, vol. 140, pp. 57-66, may 2019, doi: 10.1016/j.ibiod.2019.03.005.
S. Anne, O. Rozenbaum, P. Andreazza, and J.-L. Rouet, «Evidence of a bacterial carbonate coating on plaster samples subjected to the Calcite Bioconcept biomineralization technique,» Construction and Building Materials, vol. 24, no 6, pp. 1036-1042, 2010, doi: 10.1016/j.conbuildmat.2009.11.014.
N. K. Dhami, M. E. C. Quirin, and A. Mukherjee, «Carbonate biomineralization and heavy metal remediation by calcifying fungi isolated from karstic caves,» Ecological Engineering., vol. 103, pp. 106-117, 2017, doi: 10.1016/j.ecoleng.2017.03.007.
J. Li, K. Benzerara, S. Bernard, and O. Beyssac, «The link between biomineralization and fossilization of bacteria: Insights from field and experimental studies,» Chemical Geology, vol. 359, pp. 49-69, 2013, doi: 10.1016/j.chemgeo.2013.09.013.
X. Qian, C. Fang, M. Huang, and V. Achal, «Characterization of fungal-mediated carbonate precipitation in the biomineralization of chromate and lead from an aqueous solution and soil,» Journal Cleaner Productions, vol. 164, pp. 198-208, 2017, doi: 10.1016/j.jclepro.2017.06.195.
P. P. F. Brasileiro, R. D. C. F. S. D. Silva, F. C. P. D. R. E. Silva, Y. B. Brandao, L. A. Sarubbo, and M. Benachour, «Biomineralization of Calcium Carbonate by Bacillus Cereus for Self-healing Biocement,» Chemical Engineering Transactions, vol. 79, pp. 97-102, 2020, doi: 10.3303/CET2079017.
F. Hammes, and W. Verstraete, «Key roles of pH and calcium metabolism in microbial carbonate precipitation,» Reviews Environmental Science and Bio/Technology, vol. 1, no 1, pp. 3-7, 2002, doi: 10.1023/A:1015135629155.
J. Peckmann, J. Paul, and V. Thiel, «Bacterially mediated formation of diagenetic aragonite and native sulfur in Zechstein carbonates (Upper Permian, Central Germany),» Sedimental Geology, vol. 126, no 1, pp. 205-222, 1999, doi: 10.1016/S0037-0738(99)00041-X.
S. Wei, H. Cui, Z. Jiang, H. Liu, H. He, and N. Fang, «Biomineralization processes of calcite induced by bacteria isolated from marine sediments,» Brazilian Journal Microbiology, vol. 46, no 2, pp. 455-464, 2015, doi: 10.1590/S1517-838246220140533.
D. S. McKay, E. Gibson, K. Thomas, H. Vali, C. Romanek, S. Clemett, X. Chillier, C. Maechiling, and R. Zare, «Search for Past Life on Mars: Possible Relic Biogenic Activity in Martian Meteorite ALH84001,» Science, vol. 273, no 5277, pp. 924-930, 1996, doi: 10.1126/science.273.5277.924.
C. Rodriguez-Navarro, M. Rodriguez-Gallego, K. Ben Chekroun, and M. T. Gonzalez-Muñoz, «Conservation of ornamental stone by Myxococcus xanthus-induced carbonate biomineralization,» Applied and Environmental Microbiology, vol. 69, no 4, pp. 2182-2193, 2003, doi: 10.1128/aem.69.4.2182-2193.2003.
P. Tiano, L. Biagiotti, and G. Mastromei, «Bacterial bio-mediated calcite precipitation for monumental stones conservation: methods of evaluation,» Journal of Microbiological Methods, vol. 36, no 1-2, pp. 139-145, 1999, doi: 10.1016/s0167-7012(99)00019-6.
K. L. Bachmeier, A. E. Williams, J. R. Warmington, and S. S. Bang, «Urease activity in microbiologically-induced calcite precipitation,» Journal of Biotechnology, vol. 93, no 2, pp. 171-181, 2002, doi: 10.1016/s0168-1656(01)00393-5.
M. del R. Rodicio, y M. del C. Mendoza, «Identificación bacteriana mediante secuenciación del ARNr 16S: fundamento, metodología y aplicaciones en microbiología clínica,» Enfermedades Infecciosas y Microbiología Clínica, vol. 22, no 4, pp. 238-245, 2004.
E. Boquet, A. Boronat, and A. Ramos-Cormenzana, «Production of Calcite (Calcium Carbonate) Crystals by Soil Bacteria is a General Phenomenon,» Nature, vol. 246, n.o 5434, Art. no 5434, 1973, doi: 10.1038/246527a0.
M. Marvasi, K. Gallagher, L. Martinez, W. Molina, R. Rodríguez, and G. Vega, «Importance of B4 Medium in Determining Organomineralization Potential of Bacterial Environmental Isolates,» Geomicrobiology, vol. 29, no 10, pp. 916-924, 2012, doi: 10.1080/01490451.2011.636145.
N. Ben Omar, J. M. Arias, and M. T. González-Muñoz, «Extracellular bacterial mineralization within the context of geomicrobiology,» Microbiology Madrid Spain, vol. 13, no 2, pp. 161-172, 1997.
C. Barabesi, A. Galizzi, G. Mastromei, M. Rossi, E. Tamburini, and B. Perito, «Bacillus subtilis Gene Cluster Involved in Calcium Carbonate Biomineralization,» Journal of Bacteriology, vol. 189, no 1, pp. 228-235, 2007, doi: 10.1128/JB.01450-06.
D. A. Rasko, J. Ravel, O. Ostad, E. Helgason, R. Cer, L. Jiang, K. Shores, D. Fouts, N. Tourasse, S. Angiouli, J. Kolonay, W. Nelson, A. Kolsto, C. Fraser, and T. Read, «The genome sequence of Bacillus cereus ATCC 10987 reveals metabolic adaptations and a large plasmid related to Bacillus anthracis pXO1,» Nucleic Acids Research, vol. 32, no 3, pp. 977-988, 2004, doi: 10.1093/nar/gkh258.
E. Castañeda, and L. C. Sánchez, «Evaluation of growth of four species of the genus Bacillus sp., the first step to understand their biocontrol effect on Fusarium sp.,» Nova, vol. 14, no 26, pp. 53-62, dic. 2016.
S. Castanier, G. Le Métayer-Levrel, G. Orial, J.-F. Loubière, and J.-P. Perthuisot, «Bacterial Carbonatogenesis and Applications to Preservation and Restoration of Historic Property,» In Of Microbes and Art: The Role of Microbial Communities in the Degradation and Protection of Cultural Heritage, O. Ciferri, P. Tiano, y G. Mastromei, Eds. Boston, MA, Springer US, pp. 203-218, 2000.
M. R. Ferrer, J. Quevedo-Sarmiento, M. A. Rivadeneyra, V. Bejar, R. Delgado, and A. Ramos-Cormenzana, «Calcium carbonate precipitation by two groups of moderately halophilic microorganisms at different temperatures and salt concentrations,» Curr. Microbiology., vol. 17, no4, pp. 221-227, 1988, doi: 10.1007/BF01589456.
B. D. Eyre, A. J. Andersson, and T. Cyronak, «Benthic coral reef calcium carbonate dissolution in an acidifying ocean,» Nature Climate Change, vol. 4, n.o 11, Art. no 11, 2014, doi: 10.1038/nclimate2380.
B. B. Williams, J. L. Gidley, J. A. Guin, and R. S. Schechter, «Characterization of Liquid-Solid Reactions. Hydrochloric Acid Calcium Carbonate Reaction,» Chemistry Industrial and Engineering Chemistry Fundamentals, vol. 9, no 4, pp. 589-596, 1970, doi: 10.1021/i160036a011
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spelling Zapata Martínez, Juan Felipee468b1d6-bfd8-4d55-b462-78f4e93ea37aCardona Echeverry, Andrés094591ff-d8d6-4846-a8ba-251d2d0f3571García Ávila, Carolina456799f2-12e4-4bfa-897c-535672ce94fb2023-12-12T15:15:51Z2023-12-12T15:15:51Z2021-12-22978-958-8628-69-1https://dspace.tdea.edu.co/handle/tdea/4860978-958-8628-70-7En el ambiente existen diferentes carbonatos inorgánicos en forma de minerales y rocas importantes, entre ellos están la calcita (CaCO3). En cuanto a este compuesto se ha reportado en la literatura que la mayoría de las bacterias heterotróficas del suelo inducen la precipitación de carbonato cálcico. Este tipo de investigaciones pueden aportar solución a múltiples problemas ambientales, como lo son la erosión, ecosíntesis, la estabilización de suelos, la fijación de CO2 atmosférico a través de la formación de sedimento de carbonato y litificación, entre otros. El objetivo de este estudio fue establecer las condiciones óptimas en la curva de crecimiento de aislados nativos de Bacillus spp para la producción de cristales, en el cual se encontró que en la fase exponencial de su ciclo de crecimiento es donde los microorganismos poseen mayor capacidad precipitadora, igualmente se determinó que las condiciones óptimas para la bioprecipitación y la estabilidad de los cristales están directamente relacionadas con un pH ligeramente alcalino.1.era ed.18application/pdfspaSello Editorial Tecnológico de AntioquiaMedellínInvestigación;151135Prácticas y herramientas de sostenibilidadS. K. Ramachandran, V. Ramakrishnan, and S. S. Bang, «Remediation of Concrete Using Microorganisms,» Acid Materials Journal, vol. 98, no 1, pp. 3-9, 2001, doi: 10.14359/10154.B. Krajewska, «Urease-aided calcium carbonate mineralization for engineering applications: A review,» Journal of Advanced Research, vol. 13, pp. 59-67, 2018, doi: 10.1016/j.jare.2017.10.009.S. Weiner, and L. Addadi, «Design strategies in mineralized biological materials,» Juornal Materials Chemistry, vol. 7, no 5, pp. 689-702, ene. 1997, doi: 10.1039/A604512J.E. E. Rios-Valenciana, R. Briones-Gallardo, L. F. Cházaro-Ruiz, N. Martínez-Villegas, and L. B. Celis, «Role of indigenous microbiota from heavily contaminated sediments in the bioprecipitation of arsenic,» Journal of Hazardous Materials, vol. 339, pp. 114-121, oct. 2017, doi: 10.1016/j.jhazmat.2017.06.019.C. Lors, D. Damidot, L. Petit, A. Legrix, N. C. Tran, and B. Masson, «Bioprecipitation of a calcium carbonate – Biofilm composite on the surface of concrete for the maintenance of nuclear reactor enclosures,» Construction and Building Materials, vol. 237, p. 117618, 2020, doi: 10.1016/j.conbuildmat.2019.117618.L. Jiang, X. Liu, H. Yin, Y. Liang, H. Liu, B. Miao, Q. Peng, D. Meng, S. Wang, J. Yang, and Z. Guo, «The utilization of biomineralization technique based on microbial induced phosphate precipitation in remediation of potentially toxic ions contaminated soil: A mini review,» Ecotoxicology and Environmental Safety, vol. 191, p. 110009, 2020, doi: 10.1016/j. ecoenv.2019.110009.Z. Basaran Bundur, M. J. Kirisits, and R. D. Ferron, «Biomineralized cement-based materials: Impact of inoculating vegetative bacterial cells on hydration and strength,» Cement and Concrete Research, vol. 67, pp. 237-245, 2015, doi: 10.1016/j.cemconres.2014.10.002.V. Achal, A. Mukherjee, D. Kumari, and Q. Zhang, «Biomineralization for sustainable construction – A review of processes and applications,» Earth-Science Reviews, vol. 148, pp. 1-17, 2015, doi: 10.1016/j.earscirev.2015.05.008.V. Pasquale, S. Fiore, D. Hlayem, A. Lettino, F. Huertas, E. Chianese, and S. Duomontet, «Biomineralization of carbonates induced by the fungi Paecilomyces inflatus and Plectosphaerella cucumerina,» International Biodeterioration and Biodegradation, vol. 140, pp. 57-66, may 2019, doi: 10.1016/j.ibiod.2019.03.005.S. Anne, O. Rozenbaum, P. Andreazza, and J.-L. Rouet, «Evidence of a bacterial carbonate coating on plaster samples subjected to the Calcite Bioconcept biomineralization technique,» Construction and Building Materials, vol. 24, no 6, pp. 1036-1042, 2010, doi: 10.1016/j.conbuildmat.2009.11.014.N. K. Dhami, M. E. C. Quirin, and A. Mukherjee, «Carbonate biomineralization and heavy metal remediation by calcifying fungi isolated from karstic caves,» Ecological Engineering., vol. 103, pp. 106-117, 2017, doi: 10.1016/j.ecoleng.2017.03.007.J. Li, K. Benzerara, S. Bernard, and O. Beyssac, «The link between biomineralization and fossilization of bacteria: Insights from field and experimental studies,» Chemical Geology, vol. 359, pp. 49-69, 2013, doi: 10.1016/j.chemgeo.2013.09.013.X. Qian, C. Fang, M. Huang, and V. Achal, «Characterization of fungal-mediated carbonate precipitation in the biomineralization of chromate and lead from an aqueous solution and soil,» Journal Cleaner Productions, vol. 164, pp. 198-208, 2017, doi: 10.1016/j.jclepro.2017.06.195.P. P. F. Brasileiro, R. D. C. F. S. D. Silva, F. C. P. D. R. E. Silva, Y. B. Brandao, L. A. Sarubbo, and M. Benachour, «Biomineralization of Calcium Carbonate by Bacillus Cereus for Self-healing Biocement,» Chemical Engineering Transactions, vol. 79, pp. 97-102, 2020, doi: 10.3303/CET2079017.F. Hammes, and W. Verstraete, «Key roles of pH and calcium metabolism in microbial carbonate precipitation,» Reviews Environmental Science and Bio/Technology, vol. 1, no 1, pp. 3-7, 2002, doi: 10.1023/A:1015135629155.J. Peckmann, J. Paul, and V. Thiel, «Bacterially mediated formation of diagenetic aragonite and native sulfur in Zechstein carbonates (Upper Permian, Central Germany),» Sedimental Geology, vol. 126, no 1, pp. 205-222, 1999, doi: 10.1016/S0037-0738(99)00041-X.S. Wei, H. Cui, Z. Jiang, H. Liu, H. He, and N. Fang, «Biomineralization processes of calcite induced by bacteria isolated from marine sediments,» Brazilian Journal Microbiology, vol. 46, no 2, pp. 455-464, 2015, doi: 10.1590/S1517-838246220140533.D. S. McKay, E. Gibson, K. Thomas, H. Vali, C. Romanek, S. Clemett, X. Chillier, C. Maechiling, and R. Zare, «Search for Past Life on Mars: Possible Relic Biogenic Activity in Martian Meteorite ALH84001,» Science, vol. 273, no 5277, pp. 924-930, 1996, doi: 10.1126/science.273.5277.924.C. Rodriguez-Navarro, M. Rodriguez-Gallego, K. Ben Chekroun, and M. T. Gonzalez-Muñoz, «Conservation of ornamental stone by Myxococcus xanthus-induced carbonate biomineralization,» Applied and Environmental Microbiology, vol. 69, no 4, pp. 2182-2193, 2003, doi: 10.1128/aem.69.4.2182-2193.2003.P. Tiano, L. Biagiotti, and G. Mastromei, «Bacterial bio-mediated calcite precipitation for monumental stones conservation: methods of evaluation,» Journal of Microbiological Methods, vol. 36, no 1-2, pp. 139-145, 1999, doi: 10.1016/s0167-7012(99)00019-6.K. L. Bachmeier, A. E. Williams, J. R. Warmington, and S. S. Bang, «Urease activity in microbiologically-induced calcite precipitation,» Journal of Biotechnology, vol. 93, no 2, pp. 171-181, 2002, doi: 10.1016/s0168-1656(01)00393-5.M. del R. Rodicio, y M. del C. Mendoza, «Identificación bacteriana mediante secuenciación del ARNr 16S: fundamento, metodología y aplicaciones en microbiología clínica,» Enfermedades Infecciosas y Microbiología Clínica, vol. 22, no 4, pp. 238-245, 2004.E. Boquet, A. Boronat, and A. 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Hydrochloric Acid Calcium Carbonate Reaction,» Chemistry Industrial and Engineering Chemistry Fundamentals, vol. 9, no 4, pp. 589-596, 1970, doi: 10.1021/i160036a011Ingeniería y operaciones afinesCarbonato de calcioBacillus sppBioprecipitaciónBiomineralizaciónCurva de crecimientoCiencias ambientales e ingenieríaBioprecipitación de carbonato de calcio mediada por aislados nativos de Bacillus sppCapítulo - Parte de Librohttp://purl.org/coar/resource_type/c_3248Textinfo:eu-repo/semantics/bookParthttp://purl.org/redcol/resource_type/CAP_LIBinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85Colombiainfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Comunidad científicaEnseñanza universitaria o superiorTHUMBNAILBioprecipitación de carbonato de calcio mediada por aislados nativos de Bacillus spp.pdf.jpgBioprecipitación de carbonato de calcio mediada por aislados nativos de Bacillus spp.pdf.jpgGenerated 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de Bacillus spp.pdfapplication/pdf1277013https://dspace.tdea.edu.co/bitstream/tdea/4860/1/Bioprecipitaci%c3%b3n%20de%20carbonato%20de%20calcio%20mediada%20por%20aislados%20nativos%20de%20Bacillus%20spp.pdf577407f1f16f3c2ec32f526020832c59MD51open accesstdea/4860oai:dspace.tdea.edu.co:tdea/48602023-12-13 03:05:10.787open accessRepositorio Institucional Tecnologico de 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 incorporada en las Obras Colectivas.

b.	Distribuir copias o fonogramas de las Obras, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública, incluyéndolas como incorporadas en Obras Colectivas, según corresponda.

c.	Distribuir copias de las Obras Derivadas que se generen, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública.
Los derechos mencionados anteriormente pueden ser ejercidos en todos los medios y formatos, actualmente conocidos o que se inventen en el futuro. Los derechos antes mencionados incluyen el derecho a realizar dichas modificaciones en la medida que sean técnicamente necesarias para ejercer los derechos en otro medio o formatos, pero de otra manera usted no está autorizado para realizar obras derivadas. Todos los derechos no otorgados expresamente por el Licenciante quedan por este medio reservados, incluyendo pero sin limitarse a aquellos que se mencionan en las secciones 4(d) y 4(e).

4. Restricciones.
La licencia otorgada en la anterior Sección 3 está expresamente sujeta y limitada por las siguientes restricciones:

a.	Usted puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra sólo bajo las condiciones de esta Licencia, y Usted debe incluir una copia de esta licencia o del Identificador Universal de Recursos de la misma con cada copia de la Obra que distribuya, exhiba públicamente, ejecute públicamente o ponga a disposición pública. No es posible ofrecer o imponer ninguna condición sobre la Obra que altere o limite las condiciones de esta Licencia o el ejercicio de los derechos de los destinatarios otorgados en este documento. No es posible sublicenciar la Obra. Usted debe mantener intactos todos los avisos que hagan referencia a esta Licencia y a la cláusula de limitación de garantías. Usted no puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra con alguna medida tecnológica que controle el acceso o la utilización de ella de una forma que sea inconsistente con las condiciones de esta Licencia. Lo anterior se aplica a la Obra incorporada a una Obra Colectiva, pero esto no exige que la Obra Colectiva aparte de la obra misma quede sujeta a las condiciones de esta Licencia. Si Usted crea una Obra Colectiva, previo aviso de cualquier Licenciante debe, en la medida de lo posible, eliminar de la Obra Colectiva cualquier referencia a dicho Licenciante o al Autor Original, según lo solicitado por el Licenciante y conforme lo exige la cláusula 4(c).

b.	Usted no puede ejercer ninguno de los derechos que le han sido otorgados en la Sección 3 precedente de modo que estén principalmente destinados o directamente dirigidos a conseguir un provecho comercial o una compensación monetaria privada. El intercambio de la Obra por otras obras protegidas por derechos de autor, ya sea a través de un sistema para compartir archivos digitales (digital file-sharing) o de cualquier otra manera no será considerado como estar destinado principalmente o dirigido directamente a conseguir un provecho comercial o una compensación monetaria privada, siempre que no se realice un pago mediante una compensación monetaria en relación con el intercambio de obras protegidas por el derecho de autor.

c.	Si usted distribuye, exhibe públicamente, ejecuta públicamente o ejecuta públicamente en forma digital la Obra o cualquier Obra Derivada u Obra Colectiva, Usted debe mantener intacta toda la información de derecho de autor de la Obra y proporcionar, de forma razonable según el medio o manera que Usted esté utilizando: (i) el nombre del Autor Original si está provisto (o seudónimo, si fuere aplicable), y/o (ii) el nombre de la parte o las partes que el Autor Original y/o el Licenciante hubieren designado para la atribución (v.g., un instituto patrocinador, editorial, publicación) en la información de los derechos de autor del Licenciante, términos de servicios o de otras formas razonables; el título de la Obra si está provisto; en la medida de lo razonablemente factible y, si está provisto, el Identificador Uniforme de Recursos (Uniform Resource Identifier) que el Licenciante especifica para ser asociado con la Obra, salvo que tal URI no se refiera a la nota sobre los derechos de autor o a la información sobre el licenciamiento de la Obra; y en el caso de una Obra Derivada, atribuir el crédito identificando el uso de la Obra en la Obra Derivada (v.g., "Traducción Francesa de la Obra del Autor Original," o "Guión Cinematográfico basado en la Obra original del Autor Original"). Tal crédito puede ser implementado de cualquier forma razonable; en el caso, sin embargo, de Obras Derivadas u Obras Colectivas, tal crédito aparecerá, como mínimo, donde aparece el crédito de cualquier otro autor comparable y de una manera, al menos, tan destacada como el crédito de otro autor comparable.

d.	Para evitar toda confusión, el Licenciante aclara que, cuando la obra es una composición musical:

i.	Regalías por interpretación y ejecución bajo licencias generales. El Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública o la ejecución pública digital de la obra y de recolectar, sea individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, SAYCO), las regalías por la ejecución pública o por la ejecución pública digital de la obra (por ejemplo Webcast) licenciada bajo licencias generales, si la interpretación o ejecución de la obra está primordialmente orientada por o dirigida a la obtención de una ventaja comercial o una compensación monetaria privada.

ii.	Regalías por Fonogramas. El Licenciante se reserva el derecho exclusivo de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, los consagrados por la SAYCO), una agencia de derechos musicales o algún agente designado, las regalías por cualquier fonograma que Usted cree a partir de la obra (“versión cover”) y distribuya, en los términos del régimen de derechos de autor, si la creación o distribución de esa versión cover está primordialmente destinada o dirigida a obtener una ventaja comercial o una compensación monetaria privada.

e.	Gestión de Derechos de Autor sobre Interpretaciones y Ejecuciones Digitales (WebCasting). Para evitar toda confusión, el Licenciante aclara que, cuando la obra sea un fonograma, el Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública digital de la obra (por ejemplo, webcast) y de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, ACINPRO), las regalías por la ejecución pública digital de la obra (por ejemplo, webcast), sujeta a las disposiciones aplicables del régimen de Derecho de Autor, si esta ejecución pública digital está primordialmente dirigida a obtener una ventaja comercial o una compensación monetaria privada.

5. Representaciones, Garantías y Limitaciones de Responsabilidad.
A MENOS QUE LAS PARTES LO ACORDARAN DE OTRA FORMA POR ESCRITO, EL LICENCIANTE OFRECE LA OBRA (EN EL ESTADO EN EL QUE SE ENCUENTRA) “TAL CUAL”, SIN BRINDAR GARANTÍAS DE CLASE ALGUNA RESPECTO DE LA OBRA, YA SEA EXPRESA, IMPLÍCITA, LEGAL O CUALQUIERA OTRA, INCLUYENDO, SIN LIMITARSE A ELLAS, GARANTÍAS DE TITULARIDAD, COMERCIABILIDAD, ADAPTABILIDAD O ADECUACIÓN A PROPÓSITO DETERMINADO, AUSENCIA DE INFRACCIÓN, DE AUSENCIA DE DEFECTOS LATENTES O DE OTRO TIPO, O LA PRESENCIA O AUSENCIA DE ERRORES, SEAN O NO DESCUBRIBLES (PUEDAN O NO SER ESTOS DESCUBIERTOS). ALGUNAS JURISDICCIONES NO PERMITEN LA EXCLUSIÓN DE GARANTÍAS IMPLÍCITAS, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.

6. Limitación de responsabilidad.
A MENOS QUE LO EXIJA EXPRESAMENTE LA LEY APLICABLE, EL LICENCIANTE NO SERÁ RESPONSABLE ANTE USTED POR DAÑO ALGUNO, SEA POR RESPONSABILIDAD EXTRACONTRACTUAL, PRECONTRACTUAL O CONTRACTUAL, OBJETIVA O SUBJETIVA, SE TRATE DE DAÑOS MORALES O PATRIMONIALES, DIRECTOS O INDIRECTOS, PREVISTOS O IMPREVISTOS PRODUCIDOS POR EL USO DE ESTA LICENCIA O DE LA OBRA, AUN CUANDO EL LICENCIANTE HAYA SIDO ADVERTIDO DE LA POSIBILIDAD DE DICHOS DAÑOS. ALGUNAS LEYES NO PERMITEN LA EXCLUSIÓN DE CIERTA RESPONSABILIDAD, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.

7. Término.

a.	Esta Licencia y los derechos otorgados en virtud de ella terminarán automáticamente si Usted infringe alguna condición establecida en ella. Sin embargo, los individuos o entidades que han recibido Obras Derivadas o Colectivas de Usted de conformidad con esta Licencia, no verán terminadas sus licencias, siempre que estos individuos o entidades sigan cumpliendo íntegramente las condiciones de estas licencias. Las Secciones 1, 2, 5, 6, 7, y 8 subsistirán a cualquier terminación de esta Licencia.

b.	Sujeta a las condiciones y términos anteriores, la licencia otorgada aquí es perpetua (durante el período de vigencia de los derechos de autor de la obra). No obstante lo anterior, el Licenciante se reserva el derecho a publicar y/o estrenar la Obra bajo condiciones de licencia diferentes o a dejar de distribuirla en los términos de esta Licencia en cualquier momento; en el entendido, sin embargo, que esa elección no servirá para revocar esta licencia o que deba ser otorgada , bajo los términos de esta licencia), y esta licencia continuará en pleno vigor y efecto a menos que sea terminada como se expresa atrás. La Licencia revocada continuará siendo plenamente vigente y efectiva si no se le da término en las condiciones indicadas anteriormente.

8. Varios.

a.	Cada vez que Usted distribuya o ponga a disposición pública la Obra o una Obra Colectiva, el Licenciante ofrecerá al destinatario una licencia en los mismos términos y condiciones que la licencia otorgada a Usted bajo esta Licencia.

b.	Si alguna disposición de esta Licencia resulta invalidada o no exigible, según la legislación vigente, esto no afectará ni la validez ni la aplicabilidad del resto de condiciones de esta Licencia y, sin acción adicional por parte de los sujetos de este acuerdo, aquélla se entenderá reformada lo mínimo necesario para hacer que dicha disposición sea válida y exigible.

c.	Ningún término o disposición de esta Licencia se estimará renunciada y ninguna violación de ella será consentida a menos que esa renuncia o consentimiento sea otorgado por escrito y firmado por la parte que renuncie o consienta.

d.	Esta Licencia refleja el acuerdo pleno entre las partes respecto a la Obra aquí licenciada. No hay arreglos, acuerdos o declaraciones respecto a la Obra que no estén especificados en este documento. El Licenciante no se verá limitado por ninguna disposición adicional que pueda surgir en alguna comunicación emanada de Usted. Esta Licencia no puede ser modificada sin el consentimiento mutuo por escrito del Licenciante y Usted.
