Single-Nucleotide Polymorphisms in NOD1, RIPK2, MICB, PLCE1, TNF, and IKBKE Genes Associated with Symptomatic Dengue in Children from Colombia

Functional immunological evidence supports the impact that the host genetic variability has on the susceptibility to develop asymptomatic or symptomatic dengue infection. Children are more prone to develop severe dengue. Thus, we have evaluated possible associations between single-nucleotide polymorphisms (SNPs) located in immune genes and the development of symptomatic dengue in children from two Colombian populations with differences in genetic backgrounds and geographical features. We genotyped 15 SNPs (in 12 genes) in 298 symptomatic children and 648 healthy controls. Ancestry proportions (APs) were inferred by genotyping 29 ancestry informative markers. We observed four SNPs associated with susceptibility to develop dengue in NOD1, RIPK2, MICB, or PLCE1 genes. Conversely, we found one SNP in TNF gene and two haplotypes in the IKBKE gene associated with resistance to develop dengue. These associations were adjusted by gender, APs, and the population of origin because the association of polymorphisms may be different in admixed populations like Colombian. To our knowledge, this is the first reported association study with dengue in IKBKE, RIPK2, and NOD1 genes. We have also confirmed previously reported associations in MICB and PLCE1 genes with dengue. Overall, our results contribute to the understanding of the genetic susceptibility/resistance to develop symptomatic dengue. Nevertheless, these associations must be validated through functional analysis.

• Autores: Useche Salvador, Yerly Magnolia; Ribeiro Alves, Marcelo; Restrepo Jaramillo, Berta Nelly; Salgado García, Doris Martha Cecilia; Narváez Rojas, Carlos Fernando; Campo Nieto, Omer; Avendaño Tamayo, Efrén De Jesús; Martínez Jaramillo, Catalina; Chacón Duque, Juan Camilo; Bedoya Barrio, Gabriel de Jesús
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2018
• Institución: Tecnológico de Antioquia
• Repositorio: Repositorio Tdea
• Idioma: eng
• OAI Identifier: oai:dspace.tdea.edu.co:tdea/2925
• Acceso en línea: https://dspace.tdea.edu.co/handle/tdea/2925
• Palabra clave: Dengue; SNPs; Polymorphism, Single Nucleotide; PSNS; PSN; PNS; Polimorfismo de Nucleótido Simple; Polimorfismo de Nucleotídeo Único; Polymorphisme de nucléotide simple; IKBKE; NOD1; MICB; RIPK2
• Rights: closedAccess
• License: http://purl.org/coar/access_right/c_14cb