DNA barcoding of Lutzomyia longipalpis species complex (Diptera: Psychodidae), suggests the existence of 8 candidate species
The sand fly Lutzomyia (L.) longipalpis has been implicated as the primary vector of Leishmania infantum, the causative agent of visceral leishmaniasis VL. In addition, it has been associated with atypical cutaneous leishmaniasis transmission in the Neotropic and Central America, respectively. The e...
- Autores:
-
Contreras Gutiérrez, María Angélica
Hoyos López, Richard Onalbi
Ramos Herrera, Ana Teresa
Vélez Bernal, Iván Darío
Vivero Gómez, Rafael José
Arrivillaga Henríquez, Jazzmín
Uribe Soto, Sandra Inés
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2021
- Institución:
- Tecnológico de Antioquia
- Repositorio:
- Repositorio Tdea
- Idioma:
- eng
- OAI Identifier:
- oai:dspace.tdea.edu.co:tdea/3034
- Acceso en línea:
- https://dspace.tdea.edu.co/handle/tdea/3034
- Palabra clave:
- DNA barcoding
Codage à barres de l’ADN
Código de barras de ADN
Leishmania
Leishmania infantum
ABGD
K2P distances
Distancias K2P
Lutzomyia longipalpis s.l
Lutzomyia pseudolongipalpis
jMOTU
- Rights
- closedAccess
- License
- http://purl.org/coar/access_right/c_14cb
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DNA barcoding of Lutzomyia longipalpis species complex (Diptera: Psychodidae), suggests the existence of 8 candidate species |
| title |
DNA barcoding of Lutzomyia longipalpis species complex (Diptera: Psychodidae), suggests the existence of 8 candidate species |
| spellingShingle |
DNA barcoding of Lutzomyia longipalpis species complex (Diptera: Psychodidae), suggests the existence of 8 candidate species DNA barcoding Codage à barres de l’ADN Código de barras de ADN Leishmania Leishmania infantum ABGD K2P distances Distancias K2P Lutzomyia longipalpis s.l Lutzomyia pseudolongipalpis jMOTU |
| title_short |
DNA barcoding of Lutzomyia longipalpis species complex (Diptera: Psychodidae), suggests the existence of 8 candidate species |
| title_full |
DNA barcoding of Lutzomyia longipalpis species complex (Diptera: Psychodidae), suggests the existence of 8 candidate species |
| title_fullStr |
DNA barcoding of Lutzomyia longipalpis species complex (Diptera: Psychodidae), suggests the existence of 8 candidate species |
| title_full_unstemmed |
DNA barcoding of Lutzomyia longipalpis species complex (Diptera: Psychodidae), suggests the existence of 8 candidate species |
| title_sort |
DNA barcoding of Lutzomyia longipalpis species complex (Diptera: Psychodidae), suggests the existence of 8 candidate species |
| dc.creator.fl_str_mv |
Contreras Gutiérrez, María Angélica Hoyos López, Richard Onalbi Ramos Herrera, Ana Teresa Vélez Bernal, Iván Darío Vivero Gómez, Rafael José Arrivillaga Henríquez, Jazzmín Uribe Soto, Sandra Inés |
| dc.contributor.author.none.fl_str_mv |
Contreras Gutiérrez, María Angélica Hoyos López, Richard Onalbi Ramos Herrera, Ana Teresa Vélez Bernal, Iván Darío Vivero Gómez, Rafael José Arrivillaga Henríquez, Jazzmín Uribe Soto, Sandra Inés |
| dc.subject.agrovoc.none.fl_str_mv |
DNA barcoding Codage à barres de l’ADN Código de barras de ADN Leishmania Leishmania infantum |
| topic |
DNA barcoding Codage à barres de l’ADN Código de barras de ADN Leishmania Leishmania infantum ABGD K2P distances Distancias K2P Lutzomyia longipalpis s.l Lutzomyia pseudolongipalpis jMOTU |
| dc.subject.proposal.none.fl_str_mv |
ABGD K2P distances Distancias K2P Lutzomyia longipalpis s.l Lutzomyia pseudolongipalpis jMOTU |
| description |
The sand fly Lutzomyia (L.) longipalpis has been implicated as the primary vector of Leishmania infantum, the causative agent of visceral leishmaniasis VL. In addition, it has been associated with atypical cutaneous leishmaniasis transmission in the Neotropic and Central America, respectively. The existence of a L. longipalpis complex species has been suggested with important implications for leishmaniasis epidemiology; however, the delimitation of species conforming it remains a topic of controversy. The DNA Barcoding Initiative based on cox1 sequence variation was used to identify the MOTUs in L. longipalpis including previously described L. pseudolongipalpis. The genetic variation was analyzed based on tree and distance methods. Fifty-five haplotypes were obtained from 103 sequences which were assigned to MOTUs, with a clear separation and a high correspondence of individuals to the groups. Maximum likelihood and Bayesian phylogenetic analysis showed eight MOTUs (100% bootstrap) with high genetic divergence (12.6%). Data obtained in the present study suggest that L. longipalpis complex consists of at least 8 lineages that may represent species. It would be desirable perform additional morphological and molecular analysis of L. longipalpis from Colosó (Caribbean ecoregion) considering that specimens from that area were grouped with L. pseudolongipalpis one of the complex species previously described from Venezuela, which has not been registered in Colombia. |
| publishDate |
2021 |
| dc.date.issued.none.fl_str_mv |
2021 |
| dc.date.accessioned.none.fl_str_mv |
2023-05-24T22:18:13Z |
| dc.date.available.none.fl_str_mv |
2023-05-24T22:18:13Z |
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Artículo de revista |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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eng |
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eng |
| dc.relation.citationvolume.spa.fl_str_mv |
221 |
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Acta Tropica |
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J. Arrivillaga et al. Filogeografía del flebótomo neotropical Lutzomyia longipalpis inferida a partir de secuencias de ADN mitocondrial. Infección Genética Evol. (2002) H Noyes et al. Leishmania chagasi : parásitos genotípicamente similares de Honduras causan leishmaniasis visceral y cutánea en humanos Exp. Parasitol. (1997) CO Nzelu et al. Código de barras de ADN para la identificación de especies de flebótomos (Diptera: Psychodidae) de áreas endémicas de leishmaniasis en Perú Acta Trop. (2015) L. Romero-Ricardo et al. Código de barras de ADN para identificar especies de flebótomos (Diptera: Psychodidae) en el foco mixto de leishmaniasis del Caribe colombiano Acta Trop. (2016) SI Soto et al. Especiación y estructura poblacional en la morfoespecie Lutzomyia longipalpis (Lutz & Neiva) derivada del gen mitocondrial ND4 mol. Filogeneta Evol. (2001) FM Vigoder et al. Los cantos de amor y los polimorfismos de los genes del período indican que Lutzomyia cruzi (Mangabeira, 1938) es una especie hermana del complejo Lutzomyia longipalpis (Lutz y Neiva, 1912). Infección Genética Evol. (2010) AA Adeniran et al. Identificación de flebótomos (Diptera: Psychodidae) de áreas endémicas de leishmaniasis en el sureste de México usando código de barras de ADN Ecol. Evolución (2019) J. Alvar et al. Leishmaniasis en el mundo y estimaciones mundiales de su incidencia Más uno (2012) J Alexandre et al. Infecciones experimentales y coinfecciones con Leishmania braziliensis y Leishmania infantum en dos especies de flebotomos, Lutzomyia migonei y Lutzomyia longipalpis ciencia Reps. (2020) J. Arrivillaga et al. Lutzomyia pseudolongipalpis : la primera nueva especie dentro del complejo longipalpis (Diptera: Psychodidae: Phlebotominae) de La Rinconada, Curarigua, Estado Lara, Venezuela J.Med. Entomol. (2001) J. Arrivillaga et al. Two new populations of Lutzomyia pseudolongipalpis Arrivillaga and Feliciangeli (Diptera: Phlebotominae) vector of visceral leishmaniasis in Venezuela Neotropical Entomol. (2009) J. Arrivillaga et al. The taxonomic status of genetically divergent populations of Lutzomyia longipalpis (Diptera: Psychodidae) based on the distribution of mitochondrial and isozyme variation J. Med. Entomol. (2003) M. Ashfaq et al. Analyzing Mosquito (Diptera: Culicidae) Diversity in Pakistan by DNA Barcoding PLoS One (2014) F. Austerlitz et al. DNA barcode analysis: a comparison of phylogenetic and statistical classification methods BMC Bioinformatics (2009) J. Azpurua et al. Lutzomyia Sand Fly Diversity and Rates of Infection by Wolbachia and an Exotic Leishmania Species on Barro Colorado Island, Panama PLoS Negl.Trop. Dis. (2010) L.G. Bauzer et al. Lutzomyia longipalpis in Brazil: a complex or a single species? A mini-review Memórias do Instituto Oswaldo Cruz (2007) L.G. Bauzer et al. The period gene and genetic differentiation between three Brazilian populations of Lutzomyia longipalpis Insect Mol. Biol. (2002) V.Q. Balbino et al. Genetic structure of natural populations of the sand fly Lutzomyia longipalpis (Diptera: Psychodidae) from the Brazilian northeastern region Acta Trop. (2006) M. Blaxter et al. Defining operational taxonomic units using DNA barcode data Philos. Trans. R. Soc. Lond. Ser. B Biol. Sci. (2005) M; Bottecchia et al. Genetic divergence in the cacophony IVS6 intron among five Brazilian populations of Lutzomyia longipalpis J. Mol. Evol. (2004) R.P. Brazil The dispersion of Lutzomyia longipalpis in urban areas Rev. Soc. Bras. Med. Trop. (2013) R.T. Brumfield et al. Historical diversification of birds in northwestern South America a molecular perspective on the role of vicariant events Evolution (1996) BC Carstens et al. How to fail at species delimitation Mol. Ecol. (2013) AE. Casaril et al. Macrogeographic genetic structure of Lutzomyia longipalpis complex populations using Next-Generation Sequencing PLoS One (2019) P Cecílio et al. J. Infect. Dis. (2020) J.E. Conn et al. The biogeography and population genetics of neotropical vector species Heredity (2007) L. Cohnstaedt et al. Phylogenetics of the Phlebotominae Sand Fly Group Verrucarum (Diptera: Psychodidae: Lutzomyia) Am. J. Trop. Med. Hyg. (2011) M.A. Contreras-Gutiérrez et al. DNA barcode: A tool for identification of Lutzomyia spp. From larvae Boletín del museo entomológico Francisco Luis Gallego (2013) M.A. Contreras-Gutiérrez et al. DNA Barcoding for the Identification of Sand Fly Species (Diptera, Psychodidae, Phlebotominae) in Colombia PLoS One (2014) C.R. Davies et al. The epidemiology and control of leishmaniasis in Andean countries Cadernos de Saúde Pública (2000) A.J. Drummond et al. BEAST: Bayesian evolutionary analysis by sampling trees BMC Evol. Biol. (2007) O. Folmer et al. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates Mol. Mar. Biol. Biotechnol. (1994) D.A. Florin et al. Divergence of Lutzomyia (Psathyromyia) shannoni (Diptera: Psychodidae: Phlebotominae) is indicated by morphometric and molecular analyses when examined between taxa from the southeastern United States and southern Mexico J. Med. Entomol. (2013) E.A.B. Galati Morfologia e Taxonomia, 2.1. Classificação de Phlebotominae B.F. Giordani et al. The role of wing geometric morphometrics in the identification of sand flies within the subgenus Lutzomyia Med. Vet. Entomol. (2017) T. Hall BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT Nucleic Acids Symp. Ser. (1999) J.G. Hamilton et al. Analysis of the sex pheromone extract of individual male Lutzomyia longipalpis sand flies from six regions in Brazil Med. Vet. Entomol. (2005) P. Hebert et al. Barcoding animal life: cytochrome c oxidase subunit 1 divergence, among closely related species Proc. R. Soc. Lond. Series B (2003) B Herwaldt Leishmaniosis D.M. Hillis et al. An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis Syst. Biol. (1993) V.H. Hodgkinson et al. Mitochondrial cytochrome B variation in populations of the visceral Leishmaniasis vector Lutzomyia longipalpis across eastern Brazil Am. J. Trop. Med. Hyg. (2003) C. Hoor et al. Andean tectonics as a cause for changing drainage patterns in Miocene northern South America Geology (1995) |
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Contreras Gutiérrez, María Angélica7c9504de-e558-4dcd-86c1-81dbc9b08615Hoyos López, Richard Onalbi74e0c336-a83f-43d6-be47-e47cc07d595fRamos Herrera, Ana Teresaa34fa98f-04d3-4728-9da8-0a137f61e318Vélez Bernal, Iván Darío77ca7f43-cad5-4610-9237-a083ad9686ceVivero Gómez, Rafael Josée5a855fb-abe9-4767-a278-155d2b1484c4Arrivillaga Henríquez, Jazzmín36ee95f6-1556-4c64-a481-1e09abf9e837Uribe Soto, Sandra Inése17ba732-17e7-4923-8482-53b031aa68502023-05-24T22:18:13Z2023-05-24T22:18:13Z20210001-706Xhttps://dspace.tdea.edu.co/handle/tdea/3034The sand fly Lutzomyia (L.) longipalpis has been implicated as the primary vector of Leishmania infantum, the causative agent of visceral leishmaniasis VL. In addition, it has been associated with atypical cutaneous leishmaniasis transmission in the Neotropic and Central America, respectively. The existence of a L. longipalpis complex species has been suggested with important implications for leishmaniasis epidemiology; however, the delimitation of species conforming it remains a topic of controversy. The DNA Barcoding Initiative based on cox1 sequence variation was used to identify the MOTUs in L. longipalpis including previously described L. pseudolongipalpis. The genetic variation was analyzed based on tree and distance methods. Fifty-five haplotypes were obtained from 103 sequences which were assigned to MOTUs, with a clear separation and a high correspondence of individuals to the groups. Maximum likelihood and Bayesian phylogenetic analysis showed eight MOTUs (100% bootstrap) with high genetic divergence (12.6%). Data obtained in the present study suggest that L. longipalpis complex consists of at least 8 lineages that may represent species. It would be desirable perform additional morphological and molecular analysis of L. longipalpis from Colosó (Caribbean ecoregion) considering that specimens from that area were grouped with L. pseudolongipalpis one of the complex species previously described from Venezuela, which has not been registered in Colombia.image/jpegengElsevier BVPaíses Bajoshttps://www.sciencedirect.com/science/article/abs/pii/S0001706X21001625DNA barcoding of Lutzomyia longipalpis species complex (Diptera: Psychodidae), suggests the existence of 8 candidate speciesArtículo de revistahttp://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85Colombia221Acta TropicaJ. Arrivillaga et al. Filogeografía del flebótomo neotropical Lutzomyia longipalpis inferida a partir de secuencias de ADN mitocondrial. Infección Genética Evol. (2002)H Noyes et al. Leishmania chagasi : parásitos genotípicamente similares de Honduras causan leishmaniasis visceral y cutánea en humanos Exp. Parasitol. (1997)CO Nzelu et al. Código de barras de ADN para la identificación de especies de flebótomos (Diptera: Psychodidae) de áreas endémicas de leishmaniasis en Perú Acta Trop. (2015)L. Romero-Ricardo et al. Código de barras de ADN para identificar especies de flebótomos (Diptera: Psychodidae) en el foco mixto de leishmaniasis del Caribe colombiano Acta Trop. (2016)SI Soto et al. Especiación y estructura poblacional en la morfoespecie Lutzomyia longipalpis (Lutz & Neiva) derivada del gen mitocondrial ND4 mol. Filogeneta Evol. (2001)FM Vigoder et al. Los cantos de amor y los polimorfismos de los genes del período indican que Lutzomyia cruzi (Mangabeira, 1938) es una especie hermana del complejo Lutzomyia longipalpis (Lutz y Neiva, 1912). Infección Genética Evol. (2010)AA Adeniran et al. Identificación de flebótomos (Diptera: Psychodidae) de áreas endémicas de leishmaniasis en el sureste de México usando código de barras de ADN Ecol. Evolución (2019)J. Alvar et al. Leishmaniasis en el mundo y estimaciones mundiales de su incidencia Más uno (2012)J Alexandre et al. Infecciones experimentales y coinfecciones con Leishmania braziliensis y Leishmania infantum en dos especies de flebotomos, Lutzomyia migonei y Lutzomyia longipalpis ciencia Reps. (2020)J. Arrivillaga et al. Lutzomyia pseudolongipalpis : la primera nueva especie dentro del complejo longipalpis (Diptera: Psychodidae: Phlebotominae) de La Rinconada, Curarigua, Estado Lara, Venezuela J.Med. Entomol. (2001)J. Arrivillaga et al. Two new populations of Lutzomyia pseudolongipalpis Arrivillaga and Feliciangeli (Diptera: Phlebotominae) vector of visceral leishmaniasis in Venezuela Neotropical Entomol. (2009)J. Arrivillaga et al. The taxonomic status of genetically divergent populations of Lutzomyia longipalpis (Diptera: Psychodidae) based on the distribution of mitochondrial and isozyme variation J. Med. Entomol. (2003)M. Ashfaq et al. Analyzing Mosquito (Diptera: Culicidae) Diversity in Pakistan by DNA Barcoding PLoS One (2014)F. Austerlitz et al. DNA barcode analysis: a comparison of phylogenetic and statistical classification methods BMC Bioinformatics (2009)J. Azpurua et al. Lutzomyia Sand Fly Diversity and Rates of Infection by Wolbachia and an Exotic Leishmania Species on Barro Colorado Island, Panama PLoS Negl.Trop. Dis. (2010)L.G. Bauzer et al. Lutzomyia longipalpis in Brazil: a complex or a single species? A mini-review Memórias do Instituto Oswaldo Cruz (2007)L.G. Bauzer et al. The period gene and genetic differentiation between three Brazilian populations of Lutzomyia longipalpis Insect Mol. Biol. (2002)V.Q. Balbino et al. Genetic structure of natural populations of the sand fly Lutzomyia longipalpis (Diptera: Psychodidae) from the Brazilian northeastern region Acta Trop. (2006)M. Blaxter et al. Defining operational taxonomic units using DNA barcode data Philos. Trans. R. Soc. Lond. Ser. B Biol. Sci. (2005)M; Bottecchia et al. Genetic divergence in the cacophony IVS6 intron among five Brazilian populations of Lutzomyia longipalpis J. Mol. Evol. (2004)R.P. Brazil The dispersion of Lutzomyia longipalpis in urban areas Rev. Soc. Bras. Med. Trop. (2013)R.T. Brumfield et al. Historical diversification of birds in northwestern South America a molecular perspective on the role of vicariant events Evolution (1996)BC Carstens et al. How to fail at species delimitation Mol. Ecol. (2013)AE. Casaril et al. Macrogeographic genetic structure of Lutzomyia longipalpis complex populations using Next-Generation Sequencing PLoS One (2019)P Cecílio et al. J. Infect. Dis. (2020)J.E. Conn et al. The biogeography and population genetics of neotropical vector species Heredity (2007)L. Cohnstaedt et al. Phylogenetics of the Phlebotominae Sand Fly Group Verrucarum (Diptera: Psychodidae: Lutzomyia) Am. J. Trop. Med. Hyg. (2011)M.A. Contreras-Gutiérrez et al. DNA barcode: A tool for identification of Lutzomyia spp. From larvae Boletín del museo entomológico Francisco Luis Gallego (2013)M.A. Contreras-Gutiérrez et al. DNA Barcoding for the Identification of Sand Fly Species (Diptera, Psychodidae, Phlebotominae) in Colombia PLoS One (2014)C.R. Davies et al. The epidemiology and control of leishmaniasis in Andean countries Cadernos de Saúde Pública (2000)A.J. Drummond et al. BEAST: Bayesian evolutionary analysis by sampling trees BMC Evol. Biol. (2007)O. Folmer et al. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates Mol. Mar. Biol. Biotechnol. (1994)D.A. Florin et al. Divergence of Lutzomyia (Psathyromyia) shannoni (Diptera: Psychodidae: Phlebotominae) is indicated by morphometric and molecular analyses when examined between taxa from the southeastern United States and southern Mexico J. Med. Entomol. (2013)E.A.B. Galati Morfologia e Taxonomia, 2.1. Classificação de PhlebotominaeB.F. Giordani et al. The role of wing geometric morphometrics in the identification of sand flies within the subgenus Lutzomyia Med. Vet. Entomol. (2017)T. Hall BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT Nucleic Acids Symp. Ser. (1999)J.G. Hamilton et al. Analysis of the sex pheromone extract of individual male Lutzomyia longipalpis sand flies from six regions in Brazil Med. Vet. Entomol. (2005)P. Hebert et al. Barcoding animal life: cytochrome c oxidase subunit 1 divergence, among closely related species Proc. R. Soc. Lond. Series B (2003)B Herwaldt LeishmaniosisD.M. Hillis et al. An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis Syst. Biol. (1993)V.H. Hodgkinson et al. Mitochondrial cytochrome B variation in populations of the visceral Leishmaniasis vector Lutzomyia longipalpis across eastern Brazil Am. J. Trop. Med. Hyg. (2003)C. Hoor et al. Andean tectonics as a cause for changing drainage patterns in Miocene northern South America Geology (1995)info:eu-repo/semantics/closedAccesshttp://purl.org/coar/access_right/c_14cbDNA barcodingCodage à barres de l’ADNCódigo de barras de ADNLeishmaniaLeishmania infantumABGDK2P distancesDistancias K2PLutzomyia longipalpis s.lLutzomyia pseudolongipalpisjMOTUTHUMBNAILDNA barcoding of Lutzomyia longipalpis species complex _Diptera- Psychodidae_ , suggests the existence of 8 candidate species.jpg.jpgDNA barcoding of Lutzomyia longipalpis species complex _Diptera- Psychodidae_ , suggests the existence of 8 candidate species.jpg.jpgGenerated Thumbnailimage/jpeg12342https://dspace.tdea.edu.co/bitstream/tdea/3034/3/DNA%20barcoding%20of%c2%a0Lutzomyia%20longipalpis%c2%a0species%20complex%20_Diptera-%20Psychodidae_%20%2c%20suggests%20the%20existence%20of%208%20candidate%20species.jpg.jpg0b54c7341d32244196f3b64eabac2efdMD53open accessLICENSElicense.txtlicense.txttext/plain; charset=utf-814828https://dspace.tdea.edu.co/bitstream/tdea/3034/2/license.txt2f9959eaf5b71fae44bbf9ec84150c7aMD52open accessORIGINALDNA barcoding of Lutzomyia longipalpis species complex _Diptera- Psychodidae_ , suggests the existence of 8 candidate species.jpgDNA barcoding of Lutzomyia longipalpis species complex _Diptera- Psychodidae_ , suggests the existence of 8 candidate species.jpgDatos del documentoimage/jpeg320285https://dspace.tdea.edu.co/bitstream/tdea/3034/1/DNA%20barcoding%20of%c2%a0Lutzomyia%20longipalpis%c2%a0species%20complex%20_Diptera-%20Psychodidae_%20%2c%20suggests%20the%20existence%20of%208%20candidate%20species.jpg73190826253cd0675ebd5d2a4e484a0dMD51open accesstdea/3034oai:dspace.tdea.edu.co:tdea/30342023-05-25 03:00:16.32open accessRepositorio Institucional Tecnologico de 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 incorporada en las Obras Colectivas.

b.	Distribuir copias o fonogramas de las Obras, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública, incluyéndolas como incorporadas en Obras Colectivas, según corresponda.

c.	Distribuir copias de las Obras Derivadas que se generen, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública.
Los derechos mencionados anteriormente pueden ser ejercidos en todos los medios y formatos, actualmente conocidos o que se inventen en el futuro. Los derechos antes mencionados incluyen el derecho a realizar dichas modificaciones en la medida que sean técnicamente necesarias para ejercer los derechos en otro medio o formatos, pero de otra manera usted no está autorizado para realizar obras derivadas. Todos los derechos no otorgados expresamente por el Licenciante quedan por este medio reservados, incluyendo pero sin limitarse a aquellos que se mencionan en las secciones 4(d) y 4(e).

4. Restricciones.
La licencia otorgada en la anterior Sección 3 está expresamente sujeta y limitada por las siguientes restricciones:

a.	Usted puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra sólo bajo las condiciones de esta Licencia, y Usted debe incluir una copia de esta licencia o del Identificador Universal de Recursos de la misma con cada copia de la Obra que distribuya, exhiba públicamente, ejecute públicamente o ponga a disposición pública. No es posible ofrecer o imponer ninguna condición sobre la Obra que altere o limite las condiciones de esta Licencia o el ejercicio de los derechos de los destinatarios otorgados en este documento. No es posible sublicenciar la Obra. Usted debe mantener intactos todos los avisos que hagan referencia a esta Licencia y a la cláusula de limitación de garantías. Usted no puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra con alguna medida tecnológica que controle el acceso o la utilización de ella de una forma que sea inconsistente con las condiciones de esta Licencia. Lo anterior se aplica a la Obra incorporada a una Obra Colectiva, pero esto no exige que la Obra Colectiva aparte de la obra misma quede sujeta a las condiciones de esta Licencia. Si Usted crea una Obra Colectiva, previo aviso de cualquier Licenciante debe, en la medida de lo posible, eliminar de la Obra Colectiva cualquier referencia a dicho Licenciante o al Autor Original, según lo solicitado por el Licenciante y conforme lo exige la cláusula 4(c).

b.	Usted no puede ejercer ninguno de los derechos que le han sido otorgados en la Sección 3 precedente de modo que estén principalmente destinados o directamente dirigidos a conseguir un provecho comercial o una compensación monetaria privada. El intercambio de la Obra por otras obras protegidas por derechos de autor, ya sea a través de un sistema para compartir archivos digitales (digital file-sharing) o de cualquier otra manera no será considerado como estar destinado principalmente o dirigido directamente a conseguir un provecho comercial o una compensación monetaria privada, siempre que no se realice un pago mediante una compensación monetaria en relación con el intercambio de obras protegidas por el derecho de autor.

c.	Si usted distribuye, exhibe públicamente, ejecuta públicamente o ejecuta públicamente en forma digital la Obra o cualquier Obra Derivada u Obra Colectiva, Usted debe mantener intacta toda la información de derecho de autor de la Obra y proporcionar, de forma razonable según el medio o manera que Usted esté utilizando: (i) el nombre del Autor Original si está provisto (o seudónimo, si fuere aplicable), y/o (ii) el nombre de la parte o las partes que el Autor Original y/o el Licenciante hubieren designado para la atribución (v.g., un instituto patrocinador, editorial, publicación) en la información de los derechos de autor del Licenciante, términos de servicios o de otras formas razonables; el título de la Obra si está provisto; en la medida de lo razonablemente factible y, si está provisto, el Identificador Uniforme de Recursos (Uniform Resource Identifier) que el Licenciante especifica para ser asociado con la Obra, salvo que tal URI no se refiera a la nota sobre los derechos de autor o a la información sobre el licenciamiento de la Obra; y en el caso de una Obra Derivada, atribuir el crédito identificando el uso de la Obra en la Obra Derivada (v.g., "Traducción Francesa de la Obra del Autor Original," o "Guión Cinematográfico basado en la Obra original del Autor Original"). Tal crédito puede ser implementado de cualquier forma razonable; en el caso, sin embargo, de Obras Derivadas u Obras Colectivas, tal crédito aparecerá, como mínimo, donde aparece el crédito de cualquier otro autor comparable y de una manera, al menos, tan destacada como el crédito de otro autor comparable.

d.	Para evitar toda confusión, el Licenciante aclara que, cuando la obra es una composición musical:

i.	Regalías por interpretación y ejecución bajo licencias generales. El Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública o la ejecución pública digital de la obra y de recolectar, sea individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, SAYCO), las regalías por la ejecución pública o por la ejecución pública digital de la obra (por ejemplo Webcast) licenciada bajo licencias generales, si la interpretación o ejecución de la obra está primordialmente orientada por o dirigida a la obtención de una ventaja comercial o una compensación monetaria privada.

ii.	Regalías por Fonogramas. El Licenciante se reserva el derecho exclusivo de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, los consagrados por la SAYCO), una agencia de derechos musicales o algún agente designado, las regalías por cualquier fonograma que Usted cree a partir de la obra (“versión cover”) y distribuya, en los términos del régimen de derechos de autor, si la creación o distribución de esa versión cover está primordialmente destinada o dirigida a obtener una ventaja comercial o una compensación monetaria privada.

e.	Gestión de Derechos de Autor sobre Interpretaciones y Ejecuciones Digitales (WebCasting). Para evitar toda confusión, el Licenciante aclara que, cuando la obra sea un fonograma, el Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública digital de la obra (por ejemplo, webcast) y de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, ACINPRO), las regalías por la ejecución pública digital de la obra (por ejemplo, webcast), sujeta a las disposiciones aplicables del régimen de Derecho de Autor, si esta ejecución pública digital está primordialmente dirigida a obtener una ventaja comercial o una compensación monetaria privada.

5. Representaciones, Garantías y Limitaciones de Responsabilidad.
A MENOS QUE LAS PARTES LO ACORDARAN DE OTRA FORMA POR ESCRITO, EL LICENCIANTE OFRECE LA OBRA (EN EL ESTADO EN EL QUE SE ENCUENTRA) “TAL CUAL”, SIN BRINDAR GARANTÍAS DE CLASE ALGUNA RESPECTO DE LA OBRA, YA SEA EXPRESA, IMPLÍCITA, LEGAL O CUALQUIERA OTRA, INCLUYENDO, SIN LIMITARSE A ELLAS, GARANTÍAS DE TITULARIDAD, COMERCIABILIDAD, ADAPTABILIDAD O ADECUACIÓN A PROPÓSITO DETERMINADO, AUSENCIA DE INFRACCIÓN, DE AUSENCIA DE DEFECTOS LATENTES O DE OTRO TIPO, O LA PRESENCIA O AUSENCIA DE ERRORES, SEAN O NO DESCUBRIBLES (PUEDAN O NO SER ESTOS DESCUBIERTOS). ALGUNAS JURISDICCIONES NO PERMITEN LA EXCLUSIÓN DE GARANTÍAS IMPLÍCITAS, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.

6. Limitación de responsabilidad.
A MENOS QUE LO EXIJA EXPRESAMENTE LA LEY APLICABLE, EL LICENCIANTE NO SERÁ RESPONSABLE ANTE USTED POR DAÑO ALGUNO, SEA POR RESPONSABILIDAD EXTRACONTRACTUAL, PRECONTRACTUAL O CONTRACTUAL, OBJETIVA O SUBJETIVA, SE TRATE DE DAÑOS MORALES O PATRIMONIALES, DIRECTOS O INDIRECTOS, PREVISTOS O IMPREVISTOS PRODUCIDOS POR EL USO DE ESTA LICENCIA O DE LA OBRA, AUN CUANDO EL LICENCIANTE HAYA SIDO ADVERTIDO DE LA POSIBILIDAD DE DICHOS DAÑOS. ALGUNAS LEYES NO PERMITEN LA EXCLUSIÓN DE CIERTA RESPONSABILIDAD, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.

7. Término.

a.	Esta Licencia y los derechos otorgados en virtud de ella terminarán automáticamente si Usted infringe alguna condición establecida en ella. Sin embargo, los individuos o entidades que han recibido Obras Derivadas o Colectivas de Usted de conformidad con esta Licencia, no verán terminadas sus licencias, siempre que estos individuos o entidades sigan cumpliendo íntegramente las condiciones de estas licencias. Las Secciones 1, 2, 5, 6, 7, y 8 subsistirán a cualquier terminación de esta Licencia.

b.	Sujeta a las condiciones y términos anteriores, la licencia otorgada aquí es perpetua (durante el período de vigencia de los derechos de autor de la obra). No obstante lo anterior, el Licenciante se reserva el derecho a publicar y/o estrenar la Obra bajo condiciones de licencia diferentes o a dejar de distribuirla en los términos de esta Licencia en cualquier momento; en el entendido, sin embargo, que esa elección no servirá para revocar esta licencia o que deba ser otorgada , bajo los términos de esta licencia), y esta licencia continuará en pleno vigor y efecto a menos que sea terminada como se expresa atrás. La Licencia revocada continuará siendo plenamente vigente y efectiva si no se le da término en las condiciones indicadas anteriormente.

8. Varios.

a.	Cada vez que Usted distribuya o ponga a disposición pública la Obra o una Obra Colectiva, el Licenciante ofrecerá al destinatario una licencia en los mismos términos y condiciones que la licencia otorgada a Usted bajo esta Licencia.

b.	Si alguna disposición de esta Licencia resulta invalidada o no exigible, según la legislación vigente, esto no afectará ni la validez ni la aplicabilidad del resto de condiciones de esta Licencia y, sin acción adicional por parte de los sujetos de este acuerdo, aquélla se entenderá reformada lo mínimo necesario para hacer que dicha disposición sea válida y exigible.

c.	Ningún término o disposición de esta Licencia se estimará renunciada y ninguna violación de ella será consentida a menos que esa renuncia o consentimiento sea otorgado por escrito y firmado por la parte que renuncie o consienta.

d.	Esta Licencia refleja el acuerdo pleno entre las partes respecto a la Obra aquí licenciada. No hay arreglos, acuerdos o declaraciones respecto a la Obra que no estén especificados en este documento. El Licenciante no se verá limitado por ninguna disposición adicional que pueda surgir en alguna comunicación emanada de Usted. Esta Licencia no puede ser modificada sin el consentimiento mutuo por escrito del Licenciante y Usted.
 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