Mejoramiento de un método no comercial para la extracción de ADN de moscas de interés médico-legal
La obtención de ADN de moscas de interés médico-legal es de relevancia para una variedad de aplicaciones. Aunque existen métodos de extracción comerciales de ADN, su uso rutinario es limitado, en algunos escenarios. En este contexto, el uso de métodos no comerciales constituye una alternativa; sin e...
- Autores:
-
Maya Duque, Andrés Felipe
Durango Manrique, Yésica Sidney
Gómez Piñeres, Luz Miryam
Gómez García, Giovan Fernando
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2023
- Institución:
- Tecnológico de Antioquia
- Repositorio:
- Repositorio Tdea
- Idioma:
- spa
- OAI Identifier:
- oai:dspace.tdea.edu.co:tdea/3827
- Acceso en línea:
- https://dspace.tdea.edu.co/handle/tdea/3827
- Palabra clave:
- Entomología forense
Forensic entomology
Entomologie légale
Ácidos nucléicos
Nucleic acids
Acide nucléique
Artrópodos
Arthropods
Arthropodes
Dípteros
Diptera
Extracción de ADN
DNA extraction
Optimización
Optimisation
Optimization
- Rights
- openAccess
- License
- https://creativecommons.org/licenses/by-nc/4.0/
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Mejoramiento de un método no comercial para la extracción de ADN de moscas de interés médico-legal |
| dc.title.translated.none.fl_str_mv |
Improvement of a non-commercial method for DNA extraction from flies of medico-legal interest |
| title |
Mejoramiento de un método no comercial para la extracción de ADN de moscas de interés médico-legal |
| spellingShingle |
Mejoramiento de un método no comercial para la extracción de ADN de moscas de interés médico-legal Entomología forense Forensic entomology Entomologie légale Ácidos nucléicos Nucleic acids Acide nucléique Artrópodos Arthropods Arthropodes Dípteros Diptera Extracción de ADN DNA extraction Optimización Optimisation Optimization |
| title_short |
Mejoramiento de un método no comercial para la extracción de ADN de moscas de interés médico-legal |
| title_full |
Mejoramiento de un método no comercial para la extracción de ADN de moscas de interés médico-legal |
| title_fullStr |
Mejoramiento de un método no comercial para la extracción de ADN de moscas de interés médico-legal |
| title_full_unstemmed |
Mejoramiento de un método no comercial para la extracción de ADN de moscas de interés médico-legal |
| title_sort |
Mejoramiento de un método no comercial para la extracción de ADN de moscas de interés médico-legal |
| dc.creator.fl_str_mv |
Maya Duque, Andrés Felipe Durango Manrique, Yésica Sidney Gómez Piñeres, Luz Miryam Gómez García, Giovan Fernando |
| dc.contributor.author.none.fl_str_mv |
Maya Duque, Andrés Felipe Durango Manrique, Yésica Sidney Gómez Piñeres, Luz Miryam Gómez García, Giovan Fernando |
| dc.subject.agrovoc.none.fl_str_mv |
Entomología forense Forensic entomology Entomologie légale Ácidos nucléicos Nucleic acids Acide nucléique |
| topic |
Entomología forense Forensic entomology Entomologie légale Ácidos nucléicos Nucleic acids Acide nucléique Artrópodos Arthropods Arthropodes Dípteros Diptera Extracción de ADN DNA extraction Optimización Optimisation Optimization |
| dc.subject.decs.none.fl_str_mv |
Artrópodos Arthropods Arthropodes Dípteros Diptera |
| dc.subject.proposal.none.fl_str_mv |
Extracción de ADN DNA extraction |
| dc.subject.unesco.none.fl_str_mv |
Optimización Optimisation Optimization |
| description |
La obtención de ADN de moscas de interés médico-legal es de relevancia para una variedad de aplicaciones. Aunque existen métodos de extracción comerciales de ADN, su uso rutinario es limitado, en algunos escenarios. En este contexto, el uso de métodos no comerciales constituye una alternativa; sin embargo, su optimización es clave para mejorar el flujo de trabajo y los resultados. Este trabajo evaluó el impacto de variaciones a un método de precipitación salina sobre la concentración y la pureza del ADN recuperado. No se encontraron diferencias significativas en la concentración de ADN extraído entre los diferentes tiempos de incubación, probados durante la fase de extracción, mientras que el incremento en el volumen de etanol absoluto, en la fase de precipitación de ADN, mejoró significativamente la concentración de ADN obtenido. Las modificaciones propuestas reducen el tiempo de ejecución y la concentración de ADN obtenido comparado con el protocolo original.Palabras clave: Entomología forense; Ácidos nucleicos; Artrópodos; Extracción de ADN; Optimización. |
| publishDate |
2023 |
| dc.date.accessioned.none.fl_str_mv |
2023-08-31T20:10:21Z |
| dc.date.available.none.fl_str_mv |
2023-08-31T20:10:21Z |
| dc.date.issued.none.fl_str_mv |
2023 |
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Artículo de revista |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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Text |
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0123-4226 |
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https://dspace.tdea.edu.co/handle/tdea/3827 |
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Revista U.D.C.A Actualidad & Divulgación Científica |
| dc.relation.references.spa.fl_str_mv |
AMMAZZALORSO, A.D.; ZOLNIK, C.P.; DANIELS, T.J.;KOLOKOTRONIS, S.-O. 2015. To beat or not to beat a tick: comparison of DNA extraction methods for ticks (Ixodes scapularis). Peer J. 3:e1147.https://doi.org/10.7717/peerj.1147 ARISTIZÁBAL-BOTERO, Á.; GROOT, H.; CAMACHO,G.P.; REALPE, E.; PAREDES, M. 2016. Análisis de lassecuencias citocromo oxidasa I y espaciadores ribosomalestranscritos internos (ITS1 y 2 y 5.8S) para la identificación de especies de interés forense. Revista Entomología Mexicana. 3:695-706 AUSUBEL, F.M.; BRENT, R.; KINGSTON, R.E.; MOORE,D.D.; SEIDMAN, J.G.; SMITH, J.A.; STRUHL, K. 2003.Current protocols in molecular biology. Ed. John Wiley &Sons, Inc. (Estados Unidos). 4648p. BENÍTEZ, H.A. 2013. Sexual dimorphism. In: Moriyama, H.(ed.). Sexual Dimorphism. IntechOpen (Croatia). p.35-50. BOESENBERG-SMITH, K.A.; PESSARAKLI, M.M.; WOLK,D.M. 2012. Assessment of DNA Yield and Purity: AnOverlooked Detail of PCR Troubleshooting. ClinicalMicrobiology Newsletter. 34(1):3-6. https://doi.org/10.1016/j.clinmicnews.2011.12.002 BUENAVENTURA, E.; PAPE, T. 2013. Revision of theNew World genus Peckia Robineau-Desvoidy (Diptera: Sarcophagidae). Zootaxa. 3622(1):1-87. https://doi.org/10.11646/zootaxa.3622.1.1 BYRD, J.H.; TOMBERLIN, J.K. 2020. Forensic entomology:The utility of arthropods in legal investigations. Third edition. CRC Press. 620p. CADAVID, I.C. 2018. Extracción de ADN a partir de insectoscon buffer de macerado. En: Gómez P., L.M.; Gómez G., G.F. (eds). Del campo al laboratorio: Integración de procedimientos para el estudio de moscas. Sello Editorial Publicar-T (Medellin, Colombia). p.87-91. CARVALHO, C.J.B.; MELLO-PATIU, C.A. 2008. Key tothe adults of the most common forensic species of Diptera in South America. Revista Brasileira de Entomologia. 52(3):390-406. https://doi.org/10.1590/S0085-56262008000300012 COLLINS, F.H.; MENDEZ, M.A.; RASMUSSEN, M.O.;MEHAFFEY, P.C.; BESANSKY, N.J.; FINNERTY, V.1987. A ribosomal RNA gene probe differentiates member species of the Anopheles gambiae complex. The American Journal of Tropical Medicine and Hygiene. 37(1):37-41. https://doi.org/10.4269/ajtmh.1987.37.37 CORTÉS-SUAREZ, L.; DURANGO, Y.S.; GÓMEZ, G.F.2021. Dimorfismo sexual en la geometría alar de Muscadomestica (Diptera: Muscidae) de Colombia. Revista de la Sociedad Entomológica Argentina. 80(1):81-88. https://doi.org/10.25085/rsea.800109 DE QUEIROZ, K. 2007. Species concepts and speciesdelimitation. Systematic Biology. 56(6):879-886. https://doi.org/10.1080/10635150701701083 DURANGO, Y.; RAMÍREZ-MORA, M. 2019. FanniaRobineau-Desvoidy (Diptera: Fanniidae) of Colombia: Newspecies, identification key and updated checklist. Zootaxa. 4604(2):301-325. https://doi.org/10.11646/zootaxa.4604.2.4 EL-HELALY, M.; BALKHY, H.H.; VALLENIUS, L. 2017.Carpal tunnel syndrome among laboratory technicians inrelation to personal and ergonomic factors at work. Journal of occupational health. 59(6):513-520. https://doi.org/10.1539/joh.16-0279-OA GÓMEZ, G.F.; BICKERSMITH, S.A.; GONZÁLEZ, R.;CONN, J.E.; CORREA, M.M. 2015. Molecular taxonomyprovides new insights into Anopheles species of the neotropical Arribalzagia series. PLoS One. 10(3):1-17. https://doi.org/10.1371/journal.pone.0119488 GREEN, M.R.; SAMBROOK, J. 2016. Precipitation ofDNA with ethanol. Cold Spring Harbor Protocols.2016(12):1116-1120. https://doi.org/10.1101/pdb.prot093377 GRISALES, D.; DE CARVALHO, C.J.B. 2019. Highlandbiodiversity of Fanniidae (Insecta, Diptera): Fourteen newspecies from the Andes and Central America. Zootaxa. 4551(3):330-360. https://doi.org/10.11646/zootaxa.4551.3.4 GUO, Y.D.; CAI, J.F.; MENG, F.M.; CHANG, Y.F.; GU, Y.;LAN, L.M.; LIANG, L.; WEN, J.F. 2012. Identification of forensically important flesh flies based on a shorter fragment of the cytochrome oxidase subunit I gene in China. Medical and Veterinary Entomology. 26(3):307-313. https://doi.org/10.1111/j.1365-2915.2011.01003.x HAMMER, Ø.; HARPER, D.A.T.; RYAN, P.D. 2001. Past:Paleontological statistics software package for education anddata analysis. Paleontologia Electronica. 4(1):1-9. KUHN, R.; BÖLLMANN, J.; KRAHL, K.; BRYANT, I.M.;MARTIENSSEN, M. 2017. Comparison of ten differentDNA extraction procedures with respect to their suitability for environmental samples. Journal of Microbiological Methods. 143:78-86. https://doi.org/10.1016/j.mimet.2017.10.007 NAKANO, A.; HONDA, J. 2015. Use of DNA sequencesto identify forensically important fly species and theirdistribution in the coastal region of Central California. Forensic Science International. 253:1-13 https://doi.org/10.1016/j.forsciint.2015.05.001 NIU, C.; KEBEDE, H.; AULD, D.L.; WOODWARD, J.E.;BUROW, G.; WRIGHT, R.J. 2008. A safe inexpensivemethod to isolate high quality plant and fungal DNA in an open laboratory environment. African Journal Biotechnology. 7(16):2818-2822. NUÑEZ RODRÍGUEZ, J.; LIRIA, J. 2017. Sexual wingshape dimorphism in Piophila casei (Linnaeus, 1758Diptera: Piophilidae). Indian Journal of Forensic Medicine and Toxicology. 11(2):217-221. https://doi.org/10.5958/0973-9130.2017.00100.1 PACKER, L.; GIBBS, J.; SHEFFIELD, C.; HANNER, R.2009. DNA barcoding and the mediocrity of morphology.Molecular Ecology Resourves. 9(Suppl. 1):42-50. https://doi.org/10.1111/j.1755-0998.2009.02631.x PANIGRAHY, N.; PRIYADARSHINI, A.; SAHOO, M.M.;VERMA, A.K.; DAVEREY, A.; SAHOO, N.K. 2022. Acomprehensive review on eco-toxicity and biodegradation of phenolics: Recent progress and future outlook. Environmental Technology & Innovation. 27:102423. https://doi.org/10.1016/j.eti.2022.102423 PASETO, M.L.; DE FARIA, L.S.; MENDES, J.; LINHARES,A.X. 2019. Diversity of Sarcophagidae (Insecta, Diptera)associated with decomposing carcasses in a rural areaof the State of Minas Gerais, Brazil. EntomoBrasilis.12(3):118-125. https://doi.org/10.12741/ebrasilis.v12i3.842 PRENDINI, L.; HANNER, R.; DESALLE, R.O.B. 2002.Obtaining, storing and archiving specimens and tissuesamples for use in molecular studies. In: DeSalle, R.; Giribet, G.; Wheeler, W. (eds). Techniques in Molecular Systematics and Evolution. Springer. p.176-248. PSIFIDI, A.; DOVAS, C.I.; BRAMIS, G.; LAZOU, T.;RUSSEL, C.L.; ARSENOS, G.; BANOS, G. 2015.Comparison of eleven methods for genomic DNA extraction suitable for large-scale whole-genome genotyping and long-term DNA banking using blood samples. PLoS ONE. 10(1):e0115960. https://doi.org/10.1371/journal.pone.0115960 RATNASINGHAM, S.; HEBERT, P.D.N. 2007. Bold: Thebarcode of life data system. Molecular Ecology Notes.7(3):355-364. https://doi.org/10.1111/j.1471-8286.2007.01678.x SAAVEDRA-MATIZ, C.A.; ISABELLE, J.T.; BISKI, C.K.;DUVA, S.J.; SWEENEY, M.L.; PARKER, A.L.; YOUNG,A.J.; DIANTONIO, L.L.; KREIN, L.M.; NICHOLS,M.J.; CAGGANA, M. 2013. Cost-effective and scalableDNA extraction method from dried blood spots. ClinicalChemistry. 59(7):1045-1051. https://doi.org/10.1373/clinchem.2012.198945 STORK, N.E. 2018. How many species of insects and otherterrestrial arthropods are there on earth? Annual Review ofEntomology. 63:31-45. https://doi.org/10.1146/annurev-ento-020117-043348 XIN, Z.; CHEN, J. 2012. A high throughput DNA extractionmethod with high yield and quality. Plant Methods. 8:26. https://doi.org/10.1186/1746-4811-8-26 UAN J.; LI, M.; LIN, S. 2015. An improved DNAextraction method for efficient and quantitative recovery ofphytoplankton diversity in natural assemblages. PLoS One. 10(7):e0133060. https://doi.org/10.1371/journal.pone.0133060 |
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Maya Duque, Andrés Felipe2d28be7c-ad7b-482a-a6c8-b1993b946ecfDurango Manrique, Yésica Sidney7bc9bedf-5fbe-4769-b08e-f663dde72430Gómez Piñeres, Luz Miryamf96a058d-0c93-424a-83bc-e7068c36a151Gómez García, Giovan Fernandoba1c43f4-0c6e-4605-a4cf-720f028968022023-08-31T20:10:21Z2023-08-31T20:10:21Z20230123-4226https://dspace.tdea.edu.co/handle/tdea/38270123-4226La obtención de ADN de moscas de interés médico-legal es de relevancia para una variedad de aplicaciones. Aunque existen métodos de extracción comerciales de ADN, su uso rutinario es limitado, en algunos escenarios. En este contexto, el uso de métodos no comerciales constituye una alternativa; sin embargo, su optimización es clave para mejorar el flujo de trabajo y los resultados. Este trabajo evaluó el impacto de variaciones a un método de precipitación salina sobre la concentración y la pureza del ADN recuperado. No se encontraron diferencias significativas en la concentración de ADN extraído entre los diferentes tiempos de incubación, probados durante la fase de extracción, mientras que el incremento en el volumen de etanol absoluto, en la fase de precipitación de ADN, mejoró significativamente la concentración de ADN obtenido. Las modificaciones propuestas reducen el tiempo de ejecución y la concentración de ADN obtenido comparado con el protocolo original.Palabras clave: Entomología forense; Ácidos nucleicos; Artrópodos; Extracción de ADN; Optimización.Obtaining DNA from flies of medico-legal interest is relevant for a variety of applications. Although commercial extraction methods offer optimal DNA, their routine use is limited in some settings. In this context, the use of non-commercial methods constitutes an alternative in laboratories with limited resources however, its optimization is key to improving the workflow and the results. This work evaluated the impact of variations to a saline precipitation method on the concentration and purity of the recovered DNA. No significant differences were found in the concentration of extracted DNA between the different incubation times tested during the extraction phase. In contrast, the increased volume of absolute ethanol in the DNA precipitation phase significantly improved the concentration of DNA obtained. The proposed modifications reduce the runtime and DNA concentration obtained compared with the original protocol.Keywords: Forensic entomology; Nucleic acids; Arthropods; DNA extraction; Optimization6 páginasapplication/pdfspaUniversidad de Ciencias Aplicadas y AmbientalesColombiahttps://creativecommons.org/licenses/by-nc/4.0/Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2https://revistas.udca.edu.co/index.php/ruadc/article/view/1946Mejoramiento de un método no comercial para la extracción de ADN de moscas de interés médico-legalImprovement of a non-commercial method for DNA extraction from flies of medico-legal interestArtículo de revistahttp://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a8561126Revista U.D.C.A Actualidad & Divulgación CientíficaAMMAZZALORSO, A.D.; ZOLNIK, C.P.; DANIELS, T.J.;KOLOKOTRONIS, S.-O. 2015. To beat or not to beat a tick: comparison of DNA extraction methods for ticks (Ixodes scapularis). Peer J. 3:e1147.https://doi.org/10.7717/peerj.1147ARISTIZÁBAL-BOTERO, Á.; GROOT, H.; CAMACHO,G.P.; REALPE, E.; PAREDES, M. 2016. Análisis de lassecuencias citocromo oxidasa I y espaciadores ribosomalestranscritos internos (ITS1 y 2 y 5.8S) para la identificación de especies de interés forense. Revista Entomología Mexicana. 3:695-706AUSUBEL, F.M.; BRENT, R.; KINGSTON, R.E.; MOORE,D.D.; SEIDMAN, J.G.; SMITH, J.A.; STRUHL, K. 2003.Current protocols in molecular biology. Ed. John Wiley &Sons, Inc. (Estados Unidos). 4648p.BENÍTEZ, H.A. 2013. Sexual dimorphism. In: Moriyama, H.(ed.). Sexual Dimorphism. IntechOpen (Croatia). p.35-50.BOESENBERG-SMITH, K.A.; PESSARAKLI, M.M.; WOLK,D.M. 2012. Assessment of DNA Yield and Purity: AnOverlooked Detail of PCR Troubleshooting. ClinicalMicrobiology Newsletter. 34(1):3-6. https://doi.org/10.1016/j.clinmicnews.2011.12.002BUENAVENTURA, E.; PAPE, T. 2013. Revision of theNew World genus Peckia Robineau-Desvoidy (Diptera: Sarcophagidae). Zootaxa. 3622(1):1-87. https://doi.org/10.11646/zootaxa.3622.1.1BYRD, J.H.; TOMBERLIN, J.K. 2020. Forensic entomology:The utility of arthropods in legal investigations. Third edition. CRC Press. 620p.CADAVID, I.C. 2018. Extracción de ADN a partir de insectoscon buffer de macerado. En: Gómez P., L.M.; Gómez G., G.F. (eds). Del campo al laboratorio: Integración de procedimientos para el estudio de moscas. Sello Editorial Publicar-T (Medellin, Colombia). p.87-91.CARVALHO, C.J.B.; MELLO-PATIU, C.A. 2008. Key tothe adults of the most common forensic species of Diptera in South America. Revista Brasileira de Entomologia. 52(3):390-406. https://doi.org/10.1590/S0085-56262008000300012COLLINS, F.H.; MENDEZ, M.A.; RASMUSSEN, M.O.;MEHAFFEY, P.C.; BESANSKY, N.J.; FINNERTY, V.1987. A ribosomal RNA gene probe differentiates member species of the Anopheles gambiae complex. 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PLoS One. 10(7):e0133060. https://doi.org/10.1371/journal.pone.0133060Entomología forenseForensic entomologyEntomologie légaleÁcidos nucléicosNucleic acidsAcide nucléiqueArtrópodosArthropodsArthropodesDípterosDipteraExtracción de ADNDNA extractionOptimizaciónOptimisationOptimizationTHUMBNAILMejoramiento de un método no comercial para la extracción de ADN de moscas de interés médico-legal.pdf.jpgMejoramiento de un método no comercial para la extracción de ADN de moscas de interés médico-legal.pdf.jpgGenerated Thumbnailimage/jpeg17225https://dspace.tdea.edu.co/bitstream/tdea/3827/4/Mejoramiento%20de%20un%20m%c3%a9todo%20no%20comercial%20para%20la%20extracci%c3%b3n%20de%20ADN%20de%20moscas%20de%20inter%c3%a9s%20m%c3%a9dico-legal.pdf.jpgc8fd916e4bab29c1f388b0aec5aad6e4MD54open accessTEXTMejoramiento de un método no comercial para la extracción de ADN de moscas de interés médico-legal.pdf.txtMejoramiento de un método no comercial para la extracción de ADN de moscas de interés 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03:01:04.72An error occurred on the license name.|||https://creativecommons.org/licenses/by-nc/4.0/open accessRepositorio Institucional Tecnologico de 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 incorporada en las Obras Colectivas.

b.	Distribuir copias o fonogramas de las Obras, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública, incluyéndolas como incorporadas en Obras Colectivas, según corresponda.

c.	Distribuir copias de las Obras Derivadas que se generen, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública.
Los derechos mencionados anteriormente pueden ser ejercidos en todos los medios y formatos, actualmente conocidos o que se inventen en el futuro. Los derechos antes mencionados incluyen el derecho a realizar dichas modificaciones en la medida que sean técnicamente necesarias para ejercer los derechos en otro medio o formatos, pero de otra manera usted no está autorizado para realizar obras derivadas. Todos los derechos no otorgados expresamente por el Licenciante quedan por este medio reservados, incluyendo pero sin limitarse a aquellos que se mencionan en las secciones 4(d) y 4(e).

4. Restricciones.
La licencia otorgada en la anterior Sección 3 está expresamente sujeta y limitada por las siguientes restricciones:

a.	Usted puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra sólo bajo las condiciones de esta Licencia, y Usted debe incluir una copia de esta licencia o del Identificador Universal de Recursos de la misma con cada copia de la Obra que distribuya, exhiba públicamente, ejecute públicamente o ponga a disposición pública. No es posible ofrecer o imponer ninguna condición sobre la Obra que altere o limite las condiciones de esta Licencia o el ejercicio de los derechos de los destinatarios otorgados en este documento. No es posible sublicenciar la Obra. Usted debe mantener intactos todos los avisos que hagan referencia a esta Licencia y a la cláusula de limitación de garantías. Usted no puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra con alguna medida tecnológica que controle el acceso o la utilización de ella de una forma que sea inconsistente con las condiciones de esta Licencia. Lo anterior se aplica a la Obra incorporada a una Obra Colectiva, pero esto no exige que la Obra Colectiva aparte de la obra misma quede sujeta a las condiciones de esta Licencia. Si Usted crea una Obra Colectiva, previo aviso de cualquier Licenciante debe, en la medida de lo posible, eliminar de la Obra Colectiva cualquier referencia a dicho Licenciante o al Autor Original, según lo solicitado por el Licenciante y conforme lo exige la cláusula 4(c).

b.	Usted no puede ejercer ninguno de los derechos que le han sido otorgados en la Sección 3 precedente de modo que estén principalmente destinados o directamente dirigidos a conseguir un provecho comercial o una compensación monetaria privada. El intercambio de la Obra por otras obras protegidas por derechos de autor, ya sea a través de un sistema para compartir archivos digitales (digital file-sharing) o de cualquier otra manera no será considerado como estar destinado principalmente o dirigido directamente a conseguir un provecho comercial o una compensación monetaria privada, siempre que no se realice un pago mediante una compensación monetaria en relación con el intercambio de obras protegidas por el derecho de autor.

c.	Si usted distribuye, exhibe públicamente, ejecuta públicamente o ejecuta públicamente en forma digital la Obra o cualquier Obra Derivada u Obra Colectiva, Usted debe mantener intacta toda la información de derecho de autor de la Obra y proporcionar, de forma razonable según el medio o manera que Usted esté utilizando: (i) el nombre del Autor Original si está provisto (o seudónimo, si fuere aplicable), y/o (ii) el nombre de la parte o las partes que el Autor Original y/o el Licenciante hubieren designado para la atribución (v.g., un instituto patrocinador, editorial, publicación) en la información de los derechos de autor del Licenciante, términos de servicios o de otras formas razonables; el título de la Obra si está provisto; en la medida de lo razonablemente factible y, si está provisto, el Identificador Uniforme de Recursos (Uniform Resource Identifier) que el Licenciante especifica para ser asociado con la Obra, salvo que tal URI no se refiera a la nota sobre los derechos de autor o a la información sobre el licenciamiento de la Obra; y en el caso de una Obra Derivada, atribuir el crédito identificando el uso de la Obra en la Obra Derivada (v.g., "Traducción Francesa de la Obra del Autor Original," o "Guión Cinematográfico basado en la Obra original del Autor Original"). Tal crédito puede ser implementado de cualquier forma razonable; en el caso, sin embargo, de Obras Derivadas u Obras Colectivas, tal crédito aparecerá, como mínimo, donde aparece el crédito de cualquier otro autor comparable y de una manera, al menos, tan destacada como el crédito de otro autor comparable.

d.	Para evitar toda confusión, el Licenciante aclara que, cuando la obra es una composición musical:

i.	Regalías por interpretación y ejecución bajo licencias generales. El Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública o la ejecución pública digital de la obra y de recolectar, sea individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, SAYCO), las regalías por la ejecución pública o por la ejecución pública digital de la obra (por ejemplo Webcast) licenciada bajo licencias generales, si la interpretación o ejecución de la obra está primordialmente orientada por o dirigida a la obtención de una ventaja comercial o una compensación monetaria privada.

ii.	Regalías por Fonogramas. El Licenciante se reserva el derecho exclusivo de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, los consagrados por la SAYCO), una agencia de derechos musicales o algún agente designado, las regalías por cualquier fonograma que Usted cree a partir de la obra (“versión cover”) y distribuya, en los términos del régimen de derechos de autor, si la creación o distribución de esa versión cover está primordialmente destinada o dirigida a obtener una ventaja comercial o una compensación monetaria privada.

e.	Gestión de Derechos de Autor sobre Interpretaciones y Ejecuciones Digitales (WebCasting). Para evitar toda confusión, el Licenciante aclara que, cuando la obra sea un fonograma, el Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública digital de la obra (por ejemplo, webcast) y de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, ACINPRO), las regalías por la ejecución pública digital de la obra (por ejemplo, webcast), sujeta a las disposiciones aplicables del régimen de Derecho de Autor, si esta ejecución pública digital está primordialmente dirigida a obtener una ventaja comercial o una compensación monetaria privada.

5. Representaciones, Garantías y Limitaciones de Responsabilidad.
A MENOS QUE LAS PARTES LO ACORDARAN DE OTRA FORMA POR ESCRITO, EL LICENCIANTE OFRECE LA OBRA (EN EL ESTADO EN EL QUE SE ENCUENTRA) “TAL CUAL”, SIN BRINDAR GARANTÍAS DE CLASE ALGUNA RESPECTO DE LA OBRA, YA SEA EXPRESA, IMPLÍCITA, LEGAL O CUALQUIERA OTRA, INCLUYENDO, SIN LIMITARSE A ELLAS, GARANTÍAS DE TITULARIDAD, COMERCIABILIDAD, ADAPTABILIDAD O ADECUACIÓN A PROPÓSITO DETERMINADO, AUSENCIA DE INFRACCIÓN, DE AUSENCIA DE DEFECTOS LATENTES O DE OTRO TIPO, O LA PRESENCIA O AUSENCIA DE ERRORES, SEAN O NO DESCUBRIBLES (PUEDAN O NO SER ESTOS DESCUBIERTOS). ALGUNAS JURISDICCIONES NO PERMITEN LA EXCLUSIÓN DE GARANTÍAS IMPLÍCITAS, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.

6. Limitación de responsabilidad.
A MENOS QUE LO EXIJA EXPRESAMENTE LA LEY APLICABLE, EL LICENCIANTE NO SERÁ RESPONSABLE ANTE USTED POR DAÑO ALGUNO, SEA POR RESPONSABILIDAD EXTRACONTRACTUAL, PRECONTRACTUAL O CONTRACTUAL, OBJETIVA O SUBJETIVA, SE TRATE DE DAÑOS MORALES O PATRIMONIALES, DIRECTOS O INDIRECTOS, PREVISTOS O IMPREVISTOS PRODUCIDOS POR EL USO DE ESTA LICENCIA O DE LA OBRA, AUN CUANDO EL LICENCIANTE HAYA SIDO ADVERTIDO DE LA POSIBILIDAD DE DICHOS DAÑOS. ALGUNAS LEYES NO PERMITEN LA EXCLUSIÓN DE CIERTA RESPONSABILIDAD, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.

7. Término.

a.	Esta Licencia y los derechos otorgados en virtud de ella terminarán automáticamente si Usted infringe alguna condición establecida en ella. Sin embargo, los individuos o entidades que han recibido Obras Derivadas o Colectivas de Usted de conformidad con esta Licencia, no verán terminadas sus licencias, siempre que estos individuos o entidades sigan cumpliendo íntegramente las condiciones de estas licencias. Las Secciones 1, 2, 5, 6, 7, y 8 subsistirán a cualquier terminación de esta Licencia.

b.	Sujeta a las condiciones y términos anteriores, la licencia otorgada aquí es perpetua (durante el período de vigencia de los derechos de autor de la obra). No obstante lo anterior, el Licenciante se reserva el derecho a publicar y/o estrenar la Obra bajo condiciones de licencia diferentes o a dejar de distribuirla en los términos de esta Licencia en cualquier momento; en el entendido, sin embargo, que esa elección no servirá para revocar esta licencia o que deba ser otorgada , bajo los términos de esta licencia), y esta licencia continuará en pleno vigor y efecto a menos que sea terminada como se expresa atrás. La Licencia revocada continuará siendo plenamente vigente y efectiva si no se le da término en las condiciones indicadas anteriormente.

8. Varios.

a.	Cada vez que Usted distribuya o ponga a disposición pública la Obra o una Obra Colectiva, el Licenciante ofrecerá al destinatario una licencia en los mismos términos y condiciones que la licencia otorgada a Usted bajo esta Licencia.

b.	Si alguna disposición de esta Licencia resulta invalidada o no exigible, según la legislación vigente, esto no afectará ni la validez ni la aplicabilidad del resto de condiciones de esta Licencia y, sin acción adicional por parte de los sujetos de este acuerdo, aquélla se entenderá reformada lo mínimo necesario para hacer que dicha disposición sea válida y exigible.

c.	Ningún término o disposición de esta Licencia se estimará renunciada y ninguna violación de ella será consentida a menos que esa renuncia o consentimiento sea otorgado por escrito y firmado por la parte que renuncie o consienta.

d.	Esta Licencia refleja el acuerdo pleno entre las partes respecto a la Obra aquí licenciada. No hay arreglos, acuerdos o declaraciones respecto a la Obra que no estén especificados en este documento. El Licenciante no se verá limitado por ninguna disposición adicional que pueda surgir en alguna comunicación emanada de Usted. Esta Licencia no puede ser modificada sin el consentimiento mutuo por escrito del Licenciante y Usted.
 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