DNA barcode for identification of immature stages of sand flies (Diptera: Psychodidae) collected from natural breeding sites.
Artículo digital
- Autores:
-
Vivero, Rafael José
Bejarano, Eduar Elías
Estrada, Luis Gregorio
Flórez, Fernando
Uribe Soto, Sandra
Yamileth Edgar
Estrada, Luis Gregorio
Ortega Gómez, Edgar
Torres Gutiérrez, Carolina
Muskus López, Carlos
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2019
- Institución:
- Universidad de Sucre
- Repositorio:
- Repositorio Unisucre
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.unisucre.edu.co:001/796
- Acceso en línea:
- https://repositorio.unisucre.edu.co/handle/001/796
https://doi.org/10.11646/zootaxa.4277.2.3
- Palabra clave:
- Brumptomyia
Lutzomyia
Colombia
Cytochrome Oxidasel I
Mitochondrial DNA
- Rights
- openAccess
- License
- Derechos Reservados - Universidad de Sucre, 2019
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| dc.title.spa.fl_str_mv |
DNA barcode for identification of immature stages of sand flies (Diptera: Psychodidae) collected from natural breeding sites. |
| title |
DNA barcode for identification of immature stages of sand flies (Diptera: Psychodidae) collected from natural breeding sites. |
| spellingShingle |
DNA barcode for identification of immature stages of sand flies (Diptera: Psychodidae) collected from natural breeding sites. Brumptomyia Lutzomyia Colombia Cytochrome Oxidasel I Mitochondrial DNA |
| title_short |
DNA barcode for identification of immature stages of sand flies (Diptera: Psychodidae) collected from natural breeding sites. |
| title_full |
DNA barcode for identification of immature stages of sand flies (Diptera: Psychodidae) collected from natural breeding sites. |
| title_fullStr |
DNA barcode for identification of immature stages of sand flies (Diptera: Psychodidae) collected from natural breeding sites. |
| title_full_unstemmed |
DNA barcode for identification of immature stages of sand flies (Diptera: Psychodidae) collected from natural breeding sites. |
| title_sort |
DNA barcode for identification of immature stages of sand flies (Diptera: Psychodidae) collected from natural breeding sites. |
| dc.creator.fl_str_mv |
Vivero, Rafael José Bejarano, Eduar Elías Estrada, Luis Gregorio Flórez, Fernando Uribe Soto, Sandra Yamileth Edgar Estrada, Luis Gregorio Ortega Gómez, Edgar Torres Gutiérrez, Carolina Muskus López, Carlos |
| dc.contributor.author.spa.fl_str_mv |
Vivero, Rafael José Bejarano, Eduar Elías Estrada, Luis Gregorio Flórez, Fernando Uribe Soto, Sandra Yamileth Edgar Estrada, Luis Gregorio Ortega Gómez, Edgar Torres Gutiérrez, Carolina Muskus López, Carlos |
| dc.subject.proposal.spa.fl_str_mv |
Brumptomyia Lutzomyia Colombia Cytochrome Oxidasel I Mitochondrial DNA |
| topic |
Brumptomyia Lutzomyia Colombia Cytochrome Oxidasel I Mitochondrial DNA |
| description |
Artículo digital |
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2019 |
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2019-09-25T16:28:41Z |
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2019-09-25T16:28:41Z |
| dc.date.issued.spa.fl_str_mv |
2019-09-25 |
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Artículo de revista |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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Vivero, R.J., Bejarano, E.E., Estrada, L.G., Flórez, F., Ortega, E., Aparicio, Y., Torres, C., Uribe, S. & Muskus, C. (2017). DNA barcode for identification of immature stages of sand flies (Diptera: Psychodidae) collected from natural breeding sites. Revista zootaxa. 4277(2), 228-236. |
| dc.identifier.issn.spa.fl_str_mv |
1175-5334 |
| dc.identifier.uri.spa.fl_str_mv |
https://repositorio.unisucre.edu.co/handle/001/796 |
| dc.identifier.doi.spa.fl_str_mv |
https://doi.org/10.11646/zootaxa.4277.2.3 |
| identifier_str_mv |
Vivero, R.J., Bejarano, E.E., Estrada, L.G., Flórez, F., Ortega, E., Aparicio, Y., Torres, C., Uribe, S. & Muskus, C. (2017). DNA barcode for identification of immature stages of sand flies (Diptera: Psychodidae) collected from natural breeding sites. Revista zootaxa. 4277(2), 228-236. 1175-5334 |
| url |
https://repositorio.unisucre.edu.co/handle/001/796 https://doi.org/10.11646/zootaxa.4277.2.3 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
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Articulo de revista |
| dc.relation.references.spa.fl_str_mv |
Altschul, S.F., Madden, T.L., Schaffer, A.A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D.J. (1997) Gapped BLAST and PSIBLAST: A new generation of protein database search programs. Nucleic Acids Research, 25, 3389–3402. https://doi.org/10.1093/nar/25.17.3389. Arrivillaga, J.C., Navarro, J.C. & Feliciangeli, D. (1999) Morfología y quetotaxia del tagma cefálico larval de Lutzomyia França, 1924 (Diptera: Psychodidae): Proposición de un sistema de nomenclatura. Boletín de Entomología Venezolana, 14, 1–13. Azpurua, J., De La Cruz, D., Valderrama, A. & Windsor, D. (2010) Lutzomyia sand fly diversity and rates of infection by Wolbachia and an exotic Leishmania species on Barro Colorado Island, Panamá. Plos Neglected Tropical Diseases, 4, e627. https://doi.org/10.1371/journal.pntd.0000627. Bates, P.A., Depaquit, J., Galati, E.A., Kamhawi, S., Maroli, M., McDowell, M.A., Picado, A., Ready, P.D., Salomón, O.D., Shaw, J.J., Traub-Cseko, Y. & Warburg, A. (2015) Recent advances in phlebotomine sand fly research related to leishmaniasis control. Parasites & Vectors, 8, 131. https://doi.org/10.1186/s13071-015-0712-x. Blouin, M.S., Yowell, C.A., Courtney, C.H. & Dame, J. (1998) Substitution bias, rapid saturation, and use of mtDNA for nematode systematics. Molecular Biology and Evolution, 15, 1719–1727. https://doi.org/10.1093/oxfordjournals.molbev.a025898. |
| dc.rights.spa.fl_str_mv |
Derechos Reservados - Universidad de Sucre, 2019 |
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http://purl.org/coar/access_right/c_abf2 |
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https://creativecommons.org/licenses/by-nc/4.0/ |
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info:eu-repo/semantics/openAccess |
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Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) |
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openAccess |
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application/pdf |
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Colombia |
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Auckland, Nueva Zelanda: Revista Zootaxa, 2017. |
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https://read.qxmd.com/keyword/208412/6 |
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Universidad de Sucre |
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Vivero, Rafael Joséea89ca0def3997b4f4e08198419558c2-1Bejarano, Eduar Elíasbdf57c514088820492acdc61e67d7db9-1Estrada, Luis Gregorio726c51a10081242380693fd9c3d72564-1Flórez, Fernandoc16c9b3d9d0399d1ded83a5ab42fd2ea-1Uribe Soto, Sandra6044e8c5415ed465b16f2ae0026125dc-1Yamileth Edgar13cdcd47c07e3c2f90685ec61b8ebe2b-1Estrada, Luis Gregorio726c51a10081242380693fd9c3d72564-1Ortega Gómez, Edgara0f5b4961e82d8c129e967dfef1115b5-1Torres Gutiérrez, Carolinab7e9934477b95c101ad4528d53974920-1Muskus López, Carlos57b7074bbcf7e40281c5dcd25e24f4d0-1Colombia2019-09-25T16:28:41Z2019-09-25T16:28:41Z2019-09-25Vivero, R.J., Bejarano, E.E., Estrada, L.G., Flórez, F., Ortega, E., Aparicio, Y., Torres, C., Uribe, S. & Muskus, C. (2017). DNA barcode for identification of immature stages of sand flies (Diptera: Psychodidae) collected from natural breeding sites. Revista zootaxa. 4277(2), 228-236.1175-5334https://repositorio.unisucre.edu.co/handle/001/796https://doi.org/10.11646/zootaxa.4277.2.3Artículo digitalAlthough phlebotomine sand flies breeding sites have been identified and recorded by several studies, the microhabitats exploited by these insects remain little-known and hard to find. In this context, the difficulty of finding immature stages, and the limited number of taxonomic studies to identify immature stages of phlebotomine sand flies, are considered the major obstacles when attempting a complete inventory of Lutzomyia species. The objective of this study is to validate Cytochrome Oxidase I (Barcode region) as a marker for the identification of immature stages of Lutzomyia species recovered from natural breeding sites in Colombia. Among 142 collected sand flies, 18 immature individuals that did not complete their life cycle were identified to species level through sequencing of the COI gene. Values of K2P genetic distance between 0.002–0.031 allowed the identification of larvae at species level. The bootstrap support values (96%) in the Neighbor-Joining dendrogram were consistent for the majority of the established MOTUS of Lutzomyia atroclavata, Lutzomyia micropyga, Lutzomyia serrana, Lutzomyia cayennensis, Lutzomyia rangeliana, Lutzomyia shannoni and some species of the genus Brumptomyia. The COI gene is validated as a marker for the identification of immature stages of the genus Lutzomyia.application/pdfengAuckland, Nueva Zelanda: Revista Zootaxa, 2017.Articulo de revistaAltschul, S.F., Madden, T.L., Schaffer, A.A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D.J. (1997) Gapped BLAST and PSIBLAST: A new generation of protein database search programs. Nucleic Acids Research, 25, 3389–3402. https://doi.org/10.1093/nar/25.17.3389.Arrivillaga, J.C., Navarro, J.C. & Feliciangeli, D. (1999) Morfología y quetotaxia del tagma cefálico larval de Lutzomyia França, 1924 (Diptera: Psychodidae): Proposición de un sistema de nomenclatura. Boletín de Entomología Venezolana, 14, 1–13.Azpurua, J., De La Cruz, D., Valderrama, A. & Windsor, D. (2010) Lutzomyia sand fly diversity and rates of infection by Wolbachia and an exotic Leishmania species on Barro Colorado Island, Panamá. Plos Neglected Tropical Diseases, 4, e627. https://doi.org/10.1371/journal.pntd.0000627.Bates, P.A., Depaquit, J., Galati, E.A., Kamhawi, S., Maroli, M., McDowell, M.A., Picado, A., Ready, P.D., Salomón, O.D., Shaw, J.J., Traub-Cseko, Y. & Warburg, A. (2015) Recent advances in phlebotomine sand fly research related to leishmaniasis control. Parasites & Vectors, 8, 131. https://doi.org/10.1186/s13071-015-0712-x.Blouin, M.S., Yowell, C.A., Courtney, C.H. & Dame, J. (1998) Substitution bias, rapid saturation, and use of mtDNA for nematode systematics. Molecular Biology and Evolution, 15, 1719–1727. https://doi.org/10.1093/oxfordjournals.molbev.a025898.Derechos Reservados - Universidad de Sucre, 2019https://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)http://purl.org/coar/access_right/c_abf2https://read.qxmd.com/keyword/208412/6DNA barcode for identification of immature stages of sand flies (Diptera: Psychodidae) collected from natural breeding sites.Artículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501Texthttp://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85BrumptomyiaLutzomyiaColombiaCytochrome Oxidasel IMitochondrial DNAPublicationLICENSElicense.txtlicense.txttext/plain; charset=utf-81366https://repositorio.unisucre.edu.co/bitstreams/0171c048-c3ac-41f8-ac67-1388d721bf4a/download5f839364c91422e4b2a78812717048fbMD52TEXTDNA barcode for identification of immature stages of sand flies (Diptera Psychodidae) collected from natural breeding sites.pdf.txtDNA barcode for identification of immature stages of sand flies (Diptera Psychodidae) collected from natural breeding sites.pdf.txtExtracted texttext/plain34835https://repositorio.unisucre.edu.co/bitstreams/1880be2f-8614-4f80-8e29-64bceb2ccebc/download4b247b3cc5ccc23a5c326366d92b14e9MD55THUMBNAILDNA barcode for identification of immature stages of sand flies (Diptera Psychodidae) collected from natural breeding sites.pdf.jpgDNA barcode for identification of immature stages of sand flies (Diptera Psychodidae) collected from natural breeding sites.pdf.jpgGenerated Thumbnailimage/jpeg6333https://repositorio.unisucre.edu.co/bitstreams/bb36d3c4-edbd-4760-b0fb-192096e1136c/download27befa7e645a921f31fc8e7169291f2cMD56ORIGINALDNA barcode for identification of immature stages of sand flies (Diptera Psychodidae) collected from natural breeding sites.pdfDNA barcode for identification of immature stages of sand flies (Diptera Psychodidae) collected from natural breeding sites.pdfapplication/pdf737113https://repositorio.unisucre.edu.co/bitstreams/50023ed8-627a-4e37-ac42-4fb4fe762029/download36b7a75af8a217b61b30ce2c4d375b12MD51001/796oai:repositorio.unisucre.edu.co:001/7962024-04-17 16:30:10.944https://creativecommons.org/licenses/by-nc/4.0/Derechos Reservados - Universidad de Sucre, 2019open.accesshttps://repositorio.unisucre.edu.coRepositorio Institucional Universidad de Sucrebdigital@metabiblioteca.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 |