Three prevacuolar compartment Rab GTPases impact candida albicans hyphal growth.
Artículo digital.
- Autores:
-
Johnston, Douglas A.
Luna Tapia, Arturo
Eberle, Karen E.
Palmer, Glen E.
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2019
- Institución:
- Universidad de Sucre
- Repositorio:
- Repositorio Unisucre
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.unisucre.edu.co:001/835
- Acceso en línea:
- https://repositorio.unisucre.edu.co/handle/001/835
- Palabra clave:
- Candida albicans
Neoformans
vacuolar
- Rights
- openAccess
- License
- Derechos Reservados - Universidad de Sucre, 2019
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| dc.title.spa.fl_str_mv |
Three prevacuolar compartment Rab GTPases impact candida albicans hyphal growth. |
| title |
Three prevacuolar compartment Rab GTPases impact candida albicans hyphal growth. |
| spellingShingle |
Three prevacuolar compartment Rab GTPases impact candida albicans hyphal growth. Candida albicans Neoformans vacuolar |
| title_short |
Three prevacuolar compartment Rab GTPases impact candida albicans hyphal growth. |
| title_full |
Three prevacuolar compartment Rab GTPases impact candida albicans hyphal growth. |
| title_fullStr |
Three prevacuolar compartment Rab GTPases impact candida albicans hyphal growth. |
| title_full_unstemmed |
Three prevacuolar compartment Rab GTPases impact candida albicans hyphal growth. |
| title_sort |
Three prevacuolar compartment Rab GTPases impact candida albicans hyphal growth. |
| dc.creator.fl_str_mv |
Johnston, Douglas A. Luna Tapia, Arturo Eberle, Karen E. Palmer, Glen E. |
| dc.contributor.author.spa.fl_str_mv |
Johnston, Douglas A. Luna Tapia, Arturo Eberle, Karen E. Palmer, Glen E. |
| dc.contributor.corporatename.spa.fl_str_mv |
American society for microbiology. |
| dc.subject.proposal.spa.fl_str_mv |
Candida albicans Neoformans vacuolar |
| topic |
Candida albicans Neoformans vacuolar |
| description |
Artículo digital. |
| publishDate |
2019 |
| dc.date.accessioned.spa.fl_str_mv |
2019-10-03T21:59:59Z |
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2019-10-03T21:59:59Z |
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2019-10-03 |
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Artículo de revista |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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Johnton, D.A., Luna, A., Eberle, K.E. & Palmer, G.E. (2013). Three prevacuolar compartment Rab GTPases impact candida albicans hyphal growth. Eukaryotic Cell. 12 (7), 1039 –1050. |
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1535-9786 |
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https://repositorio.unisucre.edu.co/handle/001/835 |
| dc.identifier.doi.spa.fl_str_mv |
10.1128/EC.00359-12 |
| identifier_str_mv |
Johnton, D.A., Luna, A., Eberle, K.E. & Palmer, G.E. (2013). Three prevacuolar compartment Rab GTPases impact candida albicans hyphal growth. Eukaryotic Cell. 12 (7), 1039 –1050. 1535-9786 10.1128/EC.00359-12 |
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https://repositorio.unisucre.edu.co/handle/001/835 |
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eng |
| language |
eng |
| dc.relation.ispartof.spa.fl_str_mv |
Revista |
| dc.relation.references.spa.fl_str_mv |
Hu G, Hacham M, Waterman SR, Panepinto J, Shin S, Liu X, Gibbons J, Valyi-Nagy T, Obara K, Jaffe HA, Ohsumi Y, Williamson PR. 2008. PI3K signaling of autophagy is required for starvation tolerance and virulence of Cryptococcus neoformans. J. Clin. Invest. 118:1186 –1197. Liu X, Hu G, Panepinto J, Williamson PR. 2006. Role of a VPS41 homologue in starvation response, intracellular survival and virulence of Cryptococcus neoformans. Mol. Microbiol. 61:1132–1146. Palmer GE. 2010. Endosomal and AP-3-dependent vacuolar trafficking routes make additive contributions to Candida albicans hyphal growth and pathogenesis. Eukaryot. Cell 9:1755–1765. 4. Hilty J, Smulian AG, Newman SL. 2008. The Histoplasma capsulatum vacuolar ATPase is required for iron homeostasis, intracellular replication in macrophages and virulence in a murine model of histoplasmosis. Mol. Microbiol. 70:127–139. Roetzer A, Gratz N, Kovarik P, Schuller C. 2010. Autophagy supports Candida glabrata survival during phagocytosis. Cell. Microbiol. 12:199 – 216. |
| dc.rights.spa.fl_str_mv |
Derechos Reservados - Universidad de Sucre, 2019 |
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http://purl.org/coar/access_right/c_abf2 |
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info:eu-repo/semantics/openAccess |
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Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) |
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openAccess |
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application/pdf |
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Colombia |
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Estados Unidos: Eukaryotic Cell , 2013. |
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https://ec.asm.org/content/12/7/1039. |
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Universidad de Sucre |
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Johnston, Douglas A.f549071fd2c8899a0d5621a68b0ee495-1Luna Tapia, Arturo37137097b30746a987e4adcda146f579-1Eberle, Karen E.81a867ef93ce49f01c8b04446c8a8a05-1Palmer, Glen E.5f5dd8dc79907c8395ffb4851dee7394-1American society for microbiology.Colombia2019-10-03T21:59:59Z2019-10-03T21:59:59Z2019-10-03Johnton, D.A., Luna, A., Eberle, K.E. & Palmer, G.E. (2013). Three prevacuolar compartment Rab GTPases impact candida albicans hyphal growth. Eukaryotic Cell. 12 (7), 1039 –1050.1535-9786https://repositorio.unisucre.edu.co/handle/001/83510.1128/EC.00359-12Artículo digital.Disruption of vacuolar biogenesis in the pathogenic yeast Candida albicans causes profound defects in polarized hyphal growth. However, the precise vacuolar pathways involved in yeast-hypha differentiation have not been determined. Previously we focused on Vps21p, a Rab GTPase involved in directing vacuolar trafficking through the late endosomal prevacuolar compartment (PVC). Herein, we identify two additional Vps21p-related GTPases, Ypt52p and Ypt53p, that colocalize with Vps21p and can suppress the hyphal defects of the vps21 / mutant. Phenotypic analysis of gene deletion strains revealed that loss of both VPS21 and YPT52 causes synthetic defects in endocytic trafficking to the vacuole, as well as delivery of the virulence-associated vacuolar membrane protein Mlt1p from the Golgi compartment. Transcription of all three GTPase-encoding genes is increased under hyphal growth conditions, and overexpression of the transcription factor Ume6p is sufficient to increase the transcription of these genes. While only the vps21 / single mutant has hyphal growth defects, these were greatly exacerbated in a vps21 / ypt52 / double mutant. On the basis of relative expression levels and phenotypic analysis of gene deletion strains, Vps21p is the most important of the three GTPases, followed by Ypt52p, while Ypt53p has an only marginal impact on C. albicans physiology. Finally, disruption of a nonendosomal AP-3-dependent vacuolar trafficking pathway in the vps21 / ypt52 / mutant, further exacerbated the stress and hyphal growth defects. These findings underscore the importance of membrane trafficking through the PVC in sustaining the invasive hyphal growth form of C. albicans.application/pdfengEstados Unidos: Eukaryotic Cell , 2013.RevistaHu G, Hacham M, Waterman SR, Panepinto J, Shin S, Liu X, Gibbons J, Valyi-Nagy T, Obara K, Jaffe HA, Ohsumi Y, Williamson PR. 2008. PI3K signaling of autophagy is required for starvation tolerance and virulence of Cryptococcus neoformans. J. Clin. Invest. 118:1186 –1197.Liu X, Hu G, Panepinto J, Williamson PR. 2006. Role of a VPS41 homologue in starvation response, intracellular survival and virulence of Cryptococcus neoformans. Mol. Microbiol. 61:1132–1146.Palmer GE. 2010. Endosomal and AP-3-dependent vacuolar trafficking routes make additive contributions to Candida albicans hyphal growth and pathogenesis. Eukaryot. Cell 9:1755–1765.4. Hilty J, Smulian AG, Newman SL. 2008. The Histoplasma capsulatum vacuolar ATPase is required for iron homeostasis, intracellular replication in macrophages and virulence in a murine model of histoplasmosis. Mol. Microbiol. 70:127–139.Roetzer A, Gratz N, Kovarik P, Schuller C. 2010. Autophagy supports Candida glabrata survival during phagocytosis. Cell. Microbiol. 12:199 – 216.Derechos Reservados - Universidad de Sucre, 2019https://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)http://purl.org/coar/access_right/c_abf2https://ec.asm.org/content/12/7/1039.Three prevacuolar compartment Rab GTPases impact candida albicans hyphal growth.Artículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501Texthttp://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Candida albicansNeoformansvacuolarPublicationLICENSElicense.txtlicense.txttext/plain; charset=utf-81366https://repositorio.unisucre.edu.co/bitstreams/6e1e2bfe-0a2b-4505-a751-955d2fcc48ba/download5f839364c91422e4b2a78812717048fbMD52ORIGINALThree prevacuolar compartment Rab GTPases impact Candida albicans hyphal growth..pdfThree prevacuolar compartment Rab GTPases impact Candida albicans hyphal growth..pdfapplication/pdf2307988https://repositorio.unisucre.edu.co/bitstreams/4c8f2367-b1c5-4e17-8eac-bc2e1ebbd559/downloadfb0bb3aeb110ea9a472826d0c8555b0dMD51TEXTThree prevacuolar compartment Rab GTPases impact Candida albicans hyphal growth..pdf.txtThree prevacuolar compartment Rab GTPases impact Candida albicans hyphal growth..pdf.txtExtracted texttext/plain59989https://repositorio.unisucre.edu.co/bitstreams/21ad7103-e83b-4476-8ccd-036df0e036bb/download7cf43b5c7e79bbc7afd87aa93e05156fMD55THUMBNAILThree prevacuolar compartment Rab GTPases impact Candida albicans hyphal growth..pdf.jpgThree prevacuolar compartment Rab GTPases impact Candida albicans hyphal growth..pdf.jpgGenerated Thumbnailimage/jpeg17243https://repositorio.unisucre.edu.co/bitstreams/18dacb73-647b-4162-a64d-25a3e2b4e363/downloaded9d785188cff217c8a618d902be968eMD56001/835oai:repositorio.unisucre.edu.co:001/8352024-04-17 16:30:12.056https://creativecommons.org/licenses/by-nc/4.0/Derechos Reservados - Universidad de Sucre, 2019open.accesshttps://repositorio.unisucre.edu.coRepositorio Institucional Universidad de Sucrebdigital@metabiblioteca.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 |