Evidence for anthropophily in five species of phlebotomine sand flies (Diptera: Psychodidae) from northern Colombia, revealed by molecular identification of bloodmeals.

Artículo digital.

Autores:: Paternina, Luís E.
Verbel Vergara, Daniel
Romero Ricardo, Luís
Pérez Doria, Alveiro
Paternina Gómez, Margaret
Martínez, Lily
Bejarano, Eduar E.

Tipo de recurso:: Article of journal

Fecha de publicación:: 2019

Institución:: Universidad de Sucre

Repositorio:: Repositorio Unisucre

Idioma:: eng

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dc.title.spa.fl_str_mv	Evidence for anthropophily in five species of phlebotomine sand flies (Diptera: Psychodidae) from northern Colombia, revealed by molecular identification of bloodmeals.
title	Evidence for anthropophily in five species of phlebotomine sand flies (Diptera: Psychodidae) from northern Colombia, revealed by molecular identification of bloodmeals.
spellingShingle	Evidence for anthropophily in five species of phlebotomine sand flies (Diptera: Psychodidae) from northern Colombia, revealed by molecular identification of bloodmeals. Lutzomyia Sandflies Bloodmeals Molecular analysis Leishmaniasis Colombia
title_short	Evidence for anthropophily in five species of phlebotomine sand flies (Diptera: Psychodidae) from northern Colombia, revealed by molecular identification of bloodmeals.
title_full	Evidence for anthropophily in five species of phlebotomine sand flies (Diptera: Psychodidae) from northern Colombia, revealed by molecular identification of bloodmeals.
title_fullStr	Evidence for anthropophily in five species of phlebotomine sand flies (Diptera: Psychodidae) from northern Colombia, revealed by molecular identification of bloodmeals.
title_full_unstemmed	Evidence for anthropophily in five species of phlebotomine sand flies (Diptera: Psychodidae) from northern Colombia, revealed by molecular identification of bloodmeals.
title_sort	Evidence for anthropophily in five species of phlebotomine sand flies (Diptera: Psychodidae) from northern Colombia, revealed by molecular identification of bloodmeals.
dc.creator.fl_str_mv	Paternina, Luís E. Verbel Vergara, Daniel Romero Ricardo, Luís Pérez Doria, Alveiro Paternina Gómez, Margaret Martínez, Lily Bejarano, Eduar E.
dc.contributor.author.spa.fl_str_mv	Paternina, Luís E. Verbel Vergara, Daniel Romero Ricardo, Luís Pérez Doria, Alveiro Paternina Gómez, Margaret Martínez, Lily Bejarano, Eduar E.
dc.subject.proposal.spa.fl_str_mv	Lutzomyia Sandflies Bloodmeals Molecular analysis Leishmaniasis Colombia
topic	Lutzomyia Sandflies Bloodmeals Molecular analysis Leishmaniasis Colombia
description	Artículo digital.
publishDate	2019
dc.date.accessioned.spa.fl_str_mv	2019-11-06T16:08:15Z
dc.date.available.spa.fl_str_mv	2019-11-06T16:08:15Z
dc.date.issued.spa.fl_str_mv	2019-11-06
dc.type.spa.fl_str_mv	Artículo de revista
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dc.identifier.citation.spa.fl_str_mv	Paternina, L.E., Verbel, D., Romero, L., Pérez, A., Paternina, M., Martínez, L. & Bejarano, E.E. (2015). Evidence for anthropophily in five species of phlebotomine sand flies (Diptera: Psychodidae) from northern Colombia, revealed by molecular identification of bloodmeals. Acta Tropica. 153: 86 - 92.
dc.identifier.issn.spa.fl_str_mv	0001-706X.
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dc.identifier.doi.spa.fl_str_mv	http://dx.doi.org/doi:10.1016/j.actatropica.2015.10.005.
identifier_str_mv	Paternina, L.E., Verbel, D., Romero, L., Pérez, A., Paternina, M., Martínez, L. & Bejarano, E.E. (2015). Evidence for anthropophily in five species of phlebotomine sand flies (Diptera: Psychodidae) from northern Colombia, revealed by molecular identification of bloodmeals. Acta Tropica. 153: 86 - 92. 0001-706X.
url	https://repositorio.unisucre.edu.co/handle/001/956 http://dx.doi.org/doi:10.1016/j.actatropica.2015.10.005.
dc.language.iso.spa.fl_str_mv	eng
language	eng
dc.relation.ispartof.spa.fl_str_mv	Revista
dc.relation.references.spa.fl_str_mv	Alexander, B., 2000. Sampling methods for phlebotomine sand flies (Diptera: Psychodidae). Med. Vet. Entomol. 14, 1–13. Alexander, B., de Carvalho, R.L., McCallum, H., Pereira, M.H., 2002. Role of the domestic chicken (Gallus gallus) in the epidemiology of urban visceral leishmaniasis in Brazil. Emerg. Infect. Dis. 8, 1480–1485. Alexander, B., Lozano, C., Barker, D.C., McCann, S.H., Adler, G.H., 1998. Detection of Leishmania (Viannia) braziliensis complex in wild mammals from Colombian coffee plantations by PCR and DNA hybridization. Acta Trop. 69, 41–50. Alvar, J., Vélez, I.D., Bern, C., Herrero, M., Desjeux, P., Cano, J., Jannin, J., den Boer, M., 2012. Leishmaniasis worldwide and global estimates of its incidence. PLoS ONE 7, e35671. doi:10.1371/journal.pone.0035671. Bejarano E.E., Estrada L.G., 2015. Family Psychodidae, in: Wolff, M., Nihei, S.S., Pérez, S.P., & de Carvalho, C.J.B. (Eds.), Catalogue of Diptera of Colombia. Zootaxa, In Press.
dc.rights.spa.fl_str_mv	Derechos Reservados - Universidad de Sucre, 2019
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eu_rights_str_mv	openAccess
dc.format.mimetype.spa.fl_str_mv	application/pdf
dc.coverage.spatial.spa.fl_str_mv	Colombia
dc.publisher.spa.fl_str_mv	Reino Unido: Acta Tropica, 2015.
dc.source.spa.fl_str_mv	https://www.ncbi.nlm.nih.gov/pubmed/26464046.
institution	Universidad de Sucre
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spelling	Paternina, Luís E.0c4d2f0bc773b6185454e4490f0cfa5d-1Verbel Vergara, Danielb793e51b20f977353a51a0a8bdc63198-1Romero Ricardo, Luís085e3d48eb7050a96e9241711133c146-1Pérez Doria, Alveiro0d3ce4ee44272be00e95735466d41519-1Paternina Gómez, Margaret2830a4b985eab7ae3ded5b5482d16e22-1Martínez, Lilybe0a5878b8573ad2744724779212561c-1Bejarano, Eduar E.90dee105e4e0b5c5061b5bdadbd691bd-1Colombia2019-11-06T16:08:15Z2019-11-06T16:08:15Z2019-11-06Paternina, L.E., Verbel, D., Romero, L., Pérez, A., Paternina, M., Martínez, L. & Bejarano, E.E. (2015). Evidence for anthropophily in five species of phlebotomine sand flies (Diptera: Psychodidae) from northern Colombia, revealed by molecular identification of bloodmeals. Acta Tropica. 153: 86 - 92.0001-706X.https://repositorio.unisucre.edu.co/handle/001/956http://dx.doi.org/doi:10.1016/j.actatropica.2015.10.005.Artículo digital.Identification of the bloodmeal sources of phlebotomine sand flies is fundamental to determining which species are anthropophilic and understanding the transmission of Leishmania parasites in natural epidemiological settings. The objective of this study was to identify sand fly bloodmeals in the mixed leishmaniasis focus of the department of Sucre, northern Colombia. In all 141 engorged female sand flies were analyzed, after being captured in intradomiciliary, peridomiciliary and extradomiciliary habitats with Shannon and CDC traps and by active searching in diurnal resting sites. Bloodmeals were identified by sequencing and analysis of a 358 bp fragment of the mitochondrial gene Cytochrome b (CYB) and a 330 bp fragment of the nuclear gene prepronociceptin (PNOC). Using both genes 105 vertebrate bloodmeals were identified, with an efficiency of 72% for CYB but only 7% for PNOC. Ten species of vertebrates were identified as providing bloodmeal sources for eight sand fly species: Homo sapiens (Lutzomyia evansi, Lu. panamensis, Lu. micropyga, Lu. shannoni and Lu. atroclavata), Equus caballus (Lu. evansi, Lu. panamensis and Lu. cayennensis cayennensis), Eq. asinus (Lu. evansi and Lu. panamensis), Bos taurus 4 (Lu. evansi, Lu. panamensis and Lu. c. cayennensis), Tamandua mexicana (Lu. shannoni and Lu. trinidadensis), Proechimys guyanensis (Lu. evansi, Lu. panamensis and Lu. c. cayennensis), Mabuya sp. (Lu. micropyga), Sus scrofa (Lu. evansi and Lu. gomezi) and Gallus gallus (Lu. evansi). Cattle, donkeys, humans and pigs were significantly more important than other animals (P = 0.0001) as hosts of Lu. evansi, this being the most abundant sand fly species. The five Lutzomyia species in which blood samples of human origin were detected included Lu. micropyga and Lu. atroclavata, constituting the first evidence of anthropophily in both species.application/pdfengReino Unido: Acta Tropica, 2015.RevistaAlexander, B., 2000. Sampling methods for phlebotomine sand flies (Diptera: Psychodidae). Med. Vet. Entomol. 14, 1–13.Alexander, B., de Carvalho, R.L., McCallum, H., Pereira, M.H., 2002. Role of the domestic chicken (Gallus gallus) in the epidemiology of urban visceral leishmaniasis in Brazil. Emerg. Infect. Dis. 8, 1480–1485.Alexander, B., Lozano, C., Barker, D.C., McCann, S.H., Adler, G.H., 1998. Detection of Leishmania (Viannia) braziliensis complex in wild mammals from Colombian coffee plantations by PCR and DNA hybridization. Acta Trop. 69, 41–50.Alvar, J., Vélez, I.D., Bern, C., Herrero, M., Desjeux, P., Cano, J., Jannin, J., den Boer, M., 2012. Leishmaniasis worldwide and global estimates of its incidence. PLoS ONE 7, e35671. doi:10.1371/journal.pone.0035671.Bejarano E.E., Estrada L.G., 2015. Family Psychodidae, in: Wolff, M., Nihei, S.S., Pérez, S.P., & de Carvalho, C.J.B. (Eds.), Catalogue of Diptera of Colombia. Zootaxa, In Press.Derechos Reservados - Universidad de Sucre, 2019https://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)http://purl.org/coar/access_right/c_abf2https://www.ncbi.nlm.nih.gov/pubmed/26464046.Evidence for anthropophily in five species of phlebotomine sand flies (Diptera: Psychodidae) from northern Colombia, revealed by molecular identification of bloodmeals.Artículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501Texthttp://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85LutzomyiaSandfliesBloodmealsMolecular analysisLeishmaniasisColombiaPublicationORIGINALEvidence for anthropophily in five species of phlebotomine sand flies (Diptera Psychodidae) from northern Colombia, revealed by molecular identification of bloodmeals..pdfEvidence for anthropophily in five species of phlebotomine sand flies (Diptera Psychodidae) from northern Colombia, revealed by molecular identification of bloodmeals..pdfapplication/pdf637579https://repositorio.unisucre.edu.co/bitstreams/45291af0-074d-4ab6-a90a-e31bba4fe1e7/download2a6e83fcc180612b59af75e440a4dff4MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81366https://repositorio.unisucre.edu.co/bitstreams/70d4cf3d-9389-4276-8244-6727c5772f89/download5f839364c91422e4b2a78812717048fbMD52TEXTEvidence for anthropophily in five species of phlebotomine sand flies (Diptera Psychodidae) from northern Colombia, revealed by molecular identification of bloodmeals..pdf.txtEvidence for anthropophily in five species of phlebotomine sand flies (Diptera Psychodidae) from northern Colombia, revealed by molecular identification of bloodmeals..pdf.txtExtracted texttext/plain43149https://repositorio.unisucre.edu.co/bitstreams/59d1ff2e-1cf6-4b35-a858-b0210db224b9/download72d18e248735470f1582e43549089301MD55THUMBNAILEvidence for anthropophily in five species of phlebotomine sand flies (Diptera Psychodidae) from northern Colombia, revealed by molecular identification of bloodmeals..pdf.jpgEvidence for anthropophily in five species of phlebotomine sand flies (Diptera Psychodidae) from northern Colombia, revealed by molecular identification of bloodmeals..pdf.jpgGenerated Thumbnailimage/jpeg9329https://repositorio.unisucre.edu.co/bitstreams/ab1bf8ab-2b47-4703-bcf8-078a7821d372/downloada398a9edce7a515d20374c657c0ee359MD56001/956oai:repositorio.unisucre.edu.co:001/9562024-04-17 16:31:22.382https://creativecommons.org/licenses/by-nc/4.0/Derechos Reservados - Universidad de Sucre, 2019open.accesshttps://repositorio.unisucre.edu.coRepositorio Institucional Universidad de Sucrebdigital@metabiblioteca.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

Evidence for anthropophily in five species of phlebotomine sand flies (Diptera: Psychodidae) from northern Colombia, revealed by molecular identification of bloodmeals.

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