Design and analytical validation of a duplex PCR for Ehrlichia and Rickettsia detection in ticks.
Artículo digital
- Autores:
-
Pérez Pérez, Juan C.
Montoya Ruiz, Carolina
Arroyave Sierra, Esteban
Paternina, Luis I.
Rodas, Juan D.
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2019
- Institución:
- Universidad de Sucre
- Repositorio:
- Repositorio Unisucre
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.unisucre.edu.co:001/784
- Acceso en línea:
- https://repositorio.unisucre.edu.co/handle/001/784
- Palabra clave:
- Exclusiveness
GroEL
Inclusiveness
Robustness
rRNA 23S
- Rights
- openAccess
- License
- Derechos Reservados - Universidad de Sucre, 2019
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| dc.title.spa.fl_str_mv |
Design and analytical validation of a duplex PCR for Ehrlichia and Rickettsia detection in ticks. |
| dc.title.alternative.spa.fl_str_mv |
Diseño y validación analítica de una PCR dúplex para la detección de Ehrlichia y Rickettsia en garrapatas |
| title |
Design and analytical validation of a duplex PCR for Ehrlichia and Rickettsia detection in ticks. |
| spellingShingle |
Design and analytical validation of a duplex PCR for Ehrlichia and Rickettsia detection in ticks. Exclusiveness GroEL Inclusiveness Robustness rRNA 23S |
| title_short |
Design and analytical validation of a duplex PCR for Ehrlichia and Rickettsia detection in ticks. |
| title_full |
Design and analytical validation of a duplex PCR for Ehrlichia and Rickettsia detection in ticks. |
| title_fullStr |
Design and analytical validation of a duplex PCR for Ehrlichia and Rickettsia detection in ticks. |
| title_full_unstemmed |
Design and analytical validation of a duplex PCR for Ehrlichia and Rickettsia detection in ticks. |
| title_sort |
Design and analytical validation of a duplex PCR for Ehrlichia and Rickettsia detection in ticks. |
| dc.creator.fl_str_mv |
Pérez Pérez, Juan C. Montoya Ruiz, Carolina Arroyave Sierra, Esteban Paternina, Luis I. Rodas, Juan D. |
| dc.contributor.author.spa.fl_str_mv |
Pérez Pérez, Juan C. Montoya Ruiz, Carolina Arroyave Sierra, Esteban Paternina, Luis I. Rodas, Juan D. |
| dc.subject.proposal.spa.fl_str_mv |
Exclusiveness GroEL Inclusiveness Robustness rRNA 23S |
| topic |
Exclusiveness GroEL Inclusiveness Robustness rRNA 23S |
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Artículo digital |
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2019 |
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2019-09-24T14:51:30Z |
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2019-09-24T14:51:30Z |
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2019-09-24 |
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Artículo de revista |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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Pérez, J.C., Montoya, C., Arroyave, E., Paternina, L.E. & Rodas, J.D. (2018). Design and analytical validation of a duplex PCR for Ehrlichia and Rickettsia detection in ticks. Revista Colombiana de Ciencias Pecuarias. 31(4), 285-294. |
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0120-0690 |
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https://repositorio.unisucre.edu.co/handle/001/784 |
| dc.identifier.doi.spa.fl_str_mv |
10.17533/udea.rccp.v31n4a05 |
| identifier_str_mv |
Pérez, J.C., Montoya, C., Arroyave, E., Paternina, L.E. & Rodas, J.D. (2018). Design and analytical validation of a duplex PCR for Ehrlichia and Rickettsia detection in ticks. Revista Colombiana de Ciencias Pecuarias. 31(4), 285-294. 0120-0690 10.17533/udea.rccp.v31n4a05 |
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https://repositorio.unisucre.edu.co/handle/001/784 |
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eng |
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eng |
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Articulo de revista |
| dc.relation.references.spa.fl_str_mv |
Bowman DD. Introduction to the alpha-proteobacteria: Wolbachia and Bartonella, Rickettsia, Brucella, Ehrlichia, and Anaplasma. Top Companion Anim Med 2011; 26:173–7. doi:10.1053/j. tcam.2011.09.002. Cabezas-Cruz A, Zweygarth E, Vancova M, Broniszewska M, Grubhoffer L, Maria L, Passos F, Ribeiro M, Alberdi P, De la fuente J. Ehrlichia minasensis sp. nov., isolated from the tick Rhipicephalus microplus. Int J Syst Evol Microbiol 2016. 66:1426–1430. doi:10.1099/ijsem.0.000895. Corales JMI, Viloria VV, Venturina VM, Mingala CN. The prevalence of Ehrlichia canis, Anaplasma platys and Babesia spp. in dogs in Nueva Ecija, Philippines based on multiplex polymerase chain reaction (mPCR) assay. Ann Parasitol 2014; 60:267–272. Dantas-Torres F, Chomel BB, Otranto D. Ticks and tick-borne diseases: a One Health perspective. Trends Parasitol 2012; 28:437–46. doi:10.1016/j.pt.2012.07.003. Doyle CK, Labruna MB, Breitschwerdt EB, Tang YW, Corstvet RE, Hegarty BC, Bloch KC, Li P, Walker DH, McBride JW. Detection of medically important Ehrlichia by quantitative multicolor TaqMan real-time polymerase chain reaction of the dsb gene. J Mol Diagn 2005; 7:504–510. doi:10.1016/S1525- 1578(10)60581-8. |
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Derechos Reservados - Universidad de Sucre, 2019 |
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Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) |
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Derechos Reservados - Universidad de Sucre, 2019 https://creativecommons.org/licenses/by-nc/4.0/ Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) http://purl.org/coar/access_right/c_abf2 |
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application/pdf |
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Colombia |
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Medellín, Colombia : Revista Colombiana de Ciencias Pecuarias, 2018. |
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https://aprendeenlinea.udea.edu.co/revistas/index.php |
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Pérez Pérez, Juan C.3fecef347d24dec5d1f93a6d4ccbcc26-1Montoya Ruiz, Carolina7ad3f2103ab3a372a7272a32af83b27c-1Arroyave Sierra, Esteban2d423100bb00553af0f8ac0f32b49da7-1Paternina, Luis I.d2368ce1f17ee54906176562837d62bf-1Rodas, Juan D.7fd77d298f0779fd3cb1409f32ee3170-1Colombia2019-09-24T14:51:30Z2019-09-24T14:51:30Z2019-09-24Pérez, J.C., Montoya, C., Arroyave, E., Paternina, L.E. & Rodas, J.D. (2018). Design and analytical validation of a duplex PCR for Ehrlichia and Rickettsia detection in ticks. Revista Colombiana de Ciencias Pecuarias. 31(4), 285-294.0120-0690https://repositorio.unisucre.edu.co/handle/001/78410.17533/udea.rccp.v31n4a05Artículo digitalBackground: Ehrlichia and Rickettsia are two major rickettsial genera transmitted by ticks that affect a number of wild and domestic animal species and human populations around the world.Antecedentes: Ehrlichia e Rickettsia são dois dos principais gêneros de rickettsias transmitidos por carrapatos que infectam tanto animais selvagens quanto animais domésticos e até homens em todo o mundo.Antecedentes: Ehrlichia spp. y Rickettsia spp. son dos de los principales géneros rickettsiales transmitidos por garrapatas que afectan a animales silvestres, domésticos y humanos alrededor del mundo.application/pdfengMedellín, Colombia : Revista Colombiana de Ciencias Pecuarias, 2018.Articulo de revistaBowman DD. Introduction to the alpha-proteobacteria: Wolbachia and Bartonella, Rickettsia, Brucella, Ehrlichia, and Anaplasma. Top Companion Anim Med 2011; 26:173–7. doi:10.1053/j. tcam.2011.09.002.Cabezas-Cruz A, Zweygarth E, Vancova M, Broniszewska M, Grubhoffer L, Maria L, Passos F, Ribeiro M, Alberdi P, De la fuente J. Ehrlichia minasensis sp. nov., isolated from the tick Rhipicephalus microplus. Int J Syst Evol Microbiol 2016. 66:1426–1430. doi:10.1099/ijsem.0.000895.Corales JMI, Viloria VV, Venturina VM, Mingala CN. The prevalence of Ehrlichia canis, Anaplasma platys and Babesia spp. in dogs in Nueva Ecija, Philippines based on multiplex polymerase chain reaction (mPCR) assay. Ann Parasitol 2014; 60:267–272.Dantas-Torres F, Chomel BB, Otranto D. Ticks and tick-borne diseases: a One Health perspective. Trends Parasitol 2012; 28:437–46. doi:10.1016/j.pt.2012.07.003.Doyle CK, Labruna MB, Breitschwerdt EB, Tang YW, Corstvet RE, Hegarty BC, Bloch KC, Li P, Walker DH, McBride JW. Detection of medically important Ehrlichia by quantitative multicolor TaqMan real-time polymerase chain reaction of the dsb gene. J Mol Diagn 2005; 7:504–510. doi:10.1016/S1525- 1578(10)60581-8.Derechos Reservados - Universidad de Sucre, 2019https://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)http://purl.org/coar/access_right/c_abf2https://aprendeenlinea.udea.edu.co/revistas/index.phpDesign and analytical validation of a duplex PCR for Ehrlichia and Rickettsia detection in ticks.Diseño y validación analítica de una PCR dúplex para la detección de Ehrlichia y Rickettsia en garrapatasArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501Texthttp://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85ExclusivenessGroELInclusivenessRobustnessrRNA 23SPublicationORIGINALDesign and analytical validation of a duplex PCR for Ehrlichia and Rickettsia detection in ticks..pdfDesign and analytical validation of a duplex PCR for Ehrlichia and Rickettsia detection in ticks..pdfapplication/pdf733610https://repositorio.unisucre.edu.co/bitstreams/d1e1e883-4613-4496-bc47-95954d0a4b60/downloada2406e860ec1262eb34658d5c160a6cdMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81366https://repositorio.unisucre.edu.co/bitstreams/8bdffbaa-d812-4cd2-b798-67b93b98e0da/download5f839364c91422e4b2a78812717048fbMD52TEXTDesign and analytical validation of a duplex PCR for Ehrlichia and Rickettsia detection in ticks..pdf.txtDesign and analytical validation of a duplex PCR for Ehrlichia and Rickettsia detection in ticks..pdf.txtExtracted texttext/plain33200https://repositorio.unisucre.edu.co/bitstreams/ee6a37f3-2809-403a-b114-418199d20873/download5cb5e4d87387c9b7a8671caac8a34834MD55THUMBNAILDesign and analytical validation of a duplex PCR for Ehrlichia and Rickettsia detection in ticks..pdf.jpgDesign and analytical validation of a duplex PCR for Ehrlichia and Rickettsia detection in ticks..pdf.jpgGenerated Thumbnailimage/jpeg10024https://repositorio.unisucre.edu.co/bitstreams/020821e0-91cf-4478-a641-08782f1696e6/downloaddb27370d989ffc9feecdbef5b74b4ee5MD56001/784oai:repositorio.unisucre.edu.co:001/7842024-04-17 16:30:23.742https://creativecommons.org/licenses/by-nc/4.0/Derechos Reservados - Universidad de Sucre, 2019open.accesshttps://repositorio.unisucre.edu.coRepositorio Institucional Universidad de Sucrebdigital@metabiblioteca.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 |