Identification of rickettsia spp infection in patients with clinical suspicion of leptospirosis application of molecular techniques in a case series.
Artículo digital.
- Autores:
-
Gómez Carmago, Doris E.
Blanco, Pedro
Mora García, Gustavo
Paternina, Margareth
Caraballo, Libardo
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2019
- Institución:
- Universidad de Sucre
- Repositorio:
- Repositorio Unisucre
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.unisucre.edu.co:001/946
- Acceso en línea:
- https://repositorio.unisucre.edu.co/handle/001/946
- Palabra clave:
- Leptospira spp
Riskettsia spp
Infection
DNA
- Rights
- openAccess
- License
- Derechos Reservados - Universidad de Sucre, 2019
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Identification of rickettsia spp infection in patients with clinical suspicion of leptospirosis application of molecular techniques in a case series. |
| title |
Identification of rickettsia spp infection in patients with clinical suspicion of leptospirosis application of molecular techniques in a case series. |
| spellingShingle |
Identification of rickettsia spp infection in patients with clinical suspicion of leptospirosis application of molecular techniques in a case series. Leptospira spp Riskettsia spp Infection DNA |
| title_short |
Identification of rickettsia spp infection in patients with clinical suspicion of leptospirosis application of molecular techniques in a case series. |
| title_full |
Identification of rickettsia spp infection in patients with clinical suspicion of leptospirosis application of molecular techniques in a case series. |
| title_fullStr |
Identification of rickettsia spp infection in patients with clinical suspicion of leptospirosis application of molecular techniques in a case series. |
| title_full_unstemmed |
Identification of rickettsia spp infection in patients with clinical suspicion of leptospirosis application of molecular techniques in a case series. |
| title_sort |
Identification of rickettsia spp infection in patients with clinical suspicion of leptospirosis application of molecular techniques in a case series. |
| dc.creator.fl_str_mv |
Gómez Carmago, Doris E. Blanco, Pedro Mora García, Gustavo Paternina, Margareth Caraballo, Libardo |
| dc.contributor.author.spa.fl_str_mv |
Gómez Carmago, Doris E. Blanco, Pedro Mora García, Gustavo Paternina, Margareth Caraballo, Libardo |
| dc.contributor.corporatename.spa.fl_str_mv |
American Society of Tropical Medicine and Hygiene. |
| dc.subject.proposal.spa.fl_str_mv |
Leptospira spp Riskettsia spp Infection DNA |
| topic |
Leptospira spp Riskettsia spp Infection DNA |
| description |
Artículo digital. |
| publishDate |
2019 |
| dc.date.accessioned.spa.fl_str_mv |
2019-10-30T16:41:30Z |
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2019-10-30T16:41:30Z |
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2019-10-30 |
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Artículo de revista |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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Gómez, D.E., Blanco, P., Mora, G., Paternina, M. & Caraballo, L. (2015). Identification of rickettsia spp infection in patients with clinical suspicion of leptospirosis application of molecular techniques in a case series. American Journal of Tropical Medicine and Hygiene. 93: 322 – 322. |
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0002-9637. |
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https://repositorio.unisucre.edu.co/handle/001/946 |
| identifier_str_mv |
Gómez, D.E., Blanco, P., Mora, G., Paternina, M. & Caraballo, L. (2015). Identification of rickettsia spp infection in patients with clinical suspicion of leptospirosis application of molecular techniques in a case series. American Journal of Tropical Medicine and Hygiene. 93: 322 – 322. 0002-9637. |
| url |
https://repositorio.unisucre.edu.co/handle/001/946 |
| dc.language.iso.spa.fl_str_mv |
eng |
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eng |
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Revista |
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Derechos Reservados - Universidad de Sucre, 2019 |
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https://creativecommons.org/licenses/by-nc/4.0/ |
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info:eu-repo/semantics/openAccess |
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Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) |
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Derechos Reservados - Universidad de Sucre, 2019 https://creativecommons.org/licenses/by-nc/4.0/ Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) http://purl.org/coar/access_right/c_abf2 |
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application/pdf |
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Colombia |
| dc.publisher.spa.fl_str_mv |
Estados Unidos, American Journal of Tropical Medicine and Hygiene , 2015. |
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https://www.researchgate.net/publication/318277842. |
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Universidad de Sucre |
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Gómez Carmago, Doris E.1a7b2831285685abcac0009465bfaea8-1Blanco, Pedro53fe6e7b5af988630794acf9982e0ed5-1Mora García, Gustavo280fc03e682c84b4c529b515f52d39ff-1Paternina, Margareth9cf0d3107a2bf34a9ed0d8204d1fd611-1Caraballo, Libardo8b4812fe34331e02bfe58ef98cdabe33-1American Society of Tropical Medicine and Hygiene.Colombia2019-10-30T16:41:30Z2019-10-30T16:41:30Z2019-10-30Gómez, D.E., Blanco, P., Mora, G., Paternina, M. & Caraballo, L. (2015). Identification of rickettsia spp infection in patients with clinical suspicion of leptospirosis application of molecular techniques in a case series. American Journal of Tropical Medicine and Hygiene. 93: 322 – 322.0002-9637.https://repositorio.unisucre.edu.co/handle/001/946Artículo digital.Leptospirosis is a public health problem on the Colombian Caribbean Coast. In Cartagena, a major city in this region, this disease is endemic with high annual lethality. In clinical practice, differential diagnosis of leptospirosis with others icteric febrile syndromes is crucial to an appropriated therapeutic approach. Nevertheless, high complexity hospitals employ only serological test to confirm suspicious cases, with an elevated frequency of undetermined results. Therefore, the aim of this study was to apply molecular techniques in patients under leptospirosis suspicion. It was carried out a case series study with adults in 2013 last trimester. Patients with clinical manifestations and positive/undetermined results in serological test for leptospirosis were included. We used Polymerase Chain Reaction to detect Leptospira spp from blood samples with specific primers that amplify a DNA segment from the lipl32 gen. A total of 9 patients were included (3 women, 6 men). IgM test for leptospirosis was positive in 5 subjects and undetermined in 4 others. A 423pb product was expected, however only 3 samples showed an amplicon of 358pb. When those DNA fragments were sequenced, none of them were part of Leptospira spp. genome. Thereafter, samples were amplified for Riskettsia spp. by nested PCR for the OmpB gene. Seven samples were positive (420pb amplicon). These samples were also positive for Rickettsia spp 17KDa gene (230pb amplicon).application/pdfengEstados Unidos, American Journal of Tropical Medicine and Hygiene , 2015.RevistaDerechos Reservados - Universidad de Sucre, 2019https://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)http://purl.org/coar/access_right/c_abf2https://www.researchgate.net/publication/318277842.Identification of rickettsia spp infection in patients with clinical suspicion of leptospirosis application of molecular techniques in a case series.Artículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501Texthttp://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Leptospira sppRiskettsia sppInfectionDNAPublicationLICENSElicense.txtlicense.txttext/plain; charset=utf-81366https://repositorio.unisucre.edu.co/bitstreams/c0a9bc8a-0deb-4ddc-81fc-7f12ec947bb9/download5f839364c91422e4b2a78812717048fbMD52TEXTIdentification of rickettsia spp infection in patients with clinical suspicion of leptospirosis application of molecular techniques in a case series..pdf.txtIdentification of rickettsia spp infection in patients with clinical suspicion of leptospirosis application of molecular techniques in a case series..pdf.txtExtracted texttext/plain4194https://repositorio.unisucre.edu.co/bitstreams/4fc751e3-0242-4723-91be-53bd6342cec2/downloadf0287f7460cc29776d66d717014a7200MD55THUMBNAILIdentification of rickettsia spp infection in patients with clinical suspicion of leptospirosis application of molecular techniques in a case series..pdf.jpgIdentification of rickettsia spp infection in patients with clinical suspicion of leptospirosis application of molecular techniques in a case series..pdf.jpgGenerated Thumbnailimage/jpeg6403https://repositorio.unisucre.edu.co/bitstreams/d6ba6469-1e9f-4bb6-9972-b0fa6979eb2f/downloadf9a0cef28532f0371371a8faed381e34MD56ORIGINALIdentification of rickettsia spp infection in patients with clinical suspicion of leptospirosis application of molecular techniques in a case series..pdfIdentification of rickettsia spp infection in patients with clinical suspicion of leptospirosis application of molecular techniques in a case series..pdfapplication/pdf344279https://repositorio.unisucre.edu.co/bitstreams/d9149a40-051c-450f-b0fd-ba61301d5128/download6b3a3c8f1a4e49c79c553793ca13dd5fMD51001/946oai:repositorio.unisucre.edu.co:001/9462024-04-17 16:30:43.428https://creativecommons.org/licenses/by-nc/4.0/Derechos Reservados - Universidad de Sucre, 2019open.accesshttps://repositorio.unisucre.edu.coRepositorio Institucional Universidad de Sucrebdigital@metabiblioteca.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 |