Burkholderia cepacia aisladas de variedades de ñame con actividad antimicrobiana contra Colletotrichum gloeosporioides

Autores:
Doncel M, Pedro
Pérez-Cordero, Alexander
Tipo de recurso:
Article of journal
Fecha de publicación:
2017
Institución:
Universidad de Sucre
Repositorio:
Repositorio Unisucre
Idioma:
spa
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oai:repositorio.unisucre.edu.co:001/1505
Acceso en línea:
https://repositorio.unisucre.edu.co/handle/001/1505
https://doi.org/10.24188/recia.v9.nS.2017.518
Palabra clave:
Microorganisms endophytes
fungal phytopathogens
metabolite
inhibition
Microorganismos endófito
hongos fitopatógenos
metabolito
inhibición.
Rights
openAccess
License
https://creativecommons.org/licenses/by-nc-sa/4.0/
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dc.title.spa.fl_str_mv Burkholderia cepacia aisladas de variedades de ñame con actividad antimicrobiana contra Colletotrichum gloeosporioides
dc.title.translated.eng.fl_str_mv Burkholderia cepacia isolated from varieties of Yam with antimicrobial activity against Colletotrichum gloeosporioides
title Burkholderia cepacia aisladas de variedades de ñame con actividad antimicrobiana contra Colletotrichum gloeosporioides
spellingShingle Burkholderia cepacia aisladas de variedades de ñame con actividad antimicrobiana contra Colletotrichum gloeosporioides
Microorganisms endophytes
fungal phytopathogens
metabolite
inhibition
Microorganismos endófito
hongos fitopatógenos
metabolito
inhibición.
title_short Burkholderia cepacia aisladas de variedades de ñame con actividad antimicrobiana contra Colletotrichum gloeosporioides
title_full Burkholderia cepacia aisladas de variedades de ñame con actividad antimicrobiana contra Colletotrichum gloeosporioides
title_fullStr Burkholderia cepacia aisladas de variedades de ñame con actividad antimicrobiana contra Colletotrichum gloeosporioides
title_full_unstemmed Burkholderia cepacia aisladas de variedades de ñame con actividad antimicrobiana contra Colletotrichum gloeosporioides
title_sort Burkholderia cepacia aisladas de variedades de ñame con actividad antimicrobiana contra Colletotrichum gloeosporioides
dc.creator.fl_str_mv Doncel M, Pedro
Pérez-Cordero, Alexander
dc.contributor.author.spa.fl_str_mv Doncel M, Pedro
Pérez-Cordero, Alexander
dc.subject.eng.fl_str_mv Microorganisms endophytes
fungal phytopathogens
metabolite
inhibition
topic Microorganisms endophytes
fungal phytopathogens
metabolite
inhibition
Microorganismos endófito
hongos fitopatógenos
metabolito
inhibición.
dc.subject.spa.fl_str_mv Microorganismos endófito
hongos fitopatógenos
metabolito
inhibición.
publishDate 2017
dc.date.accessioned.none.fl_str_mv 2017-05-05 00:00:00
2022-07-01T17:15:50Z
dc.date.available.none.fl_str_mv 2017-05-05 00:00:00
2022-07-01T17:15:50Z
dc.date.issued.none.fl_str_mv 2017-05-05
dc.type.spa.fl_str_mv Artículo de revista
dc.type.eng.fl_str_mv Journal article
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dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/article
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/publishedVersion
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http://purl.org/coar/resource_type/c_6501
dc.type.content.spa.fl_str_mv Text
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dc.identifier.doi.none.fl_str_mv 10.24188/recia.v9.nS.2017.518
dc.identifier.eissn.none.fl_str_mv 2027-4297
dc.identifier.url.none.fl_str_mv https://doi.org/10.24188/recia.v9.nS.2017.518
url https://repositorio.unisucre.edu.co/handle/001/1505
https://doi.org/10.24188/recia.v9.nS.2017.518
identifier_str_mv 10.24188/recia.v9.nS.2017.518
2027-4297
dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.references.spa.fl_str_mv ABANG, M.M.; WINTER, S.; MIGNOUNA, H.D.; GREEN, K.R.; ASIEDU, R. 2003). Molecular taxonomic, epidemiological and population genetic approaches to understanding yam anthracnose disease. Afr J Biotechnol 2, 486–496.
ABE, M.; NAKAZAWA T. 1993. Characterization of hemolytic and antifungal substance, cepalycin, from Pseudomonas cepacia. Microbiol Inmunol. 38: 1-9.
ALEMÁN, I.; SÁNCHEZ, J.; SEALEY, M.; ROJAS, J.; LÓPEZ, G. 2003. Empleo de una cepa de Burkholderia cepacia en el control de la mancha azul en la madera de pino caribe (Pinus caribaea). Ciencia. 11: 39-46. AMUSA, N.; ADEGBITE, A.; MUHAMMED, S; BAIYEWU, R. 2003. Yam disease and its management in Nigeria. African Journals of Biotecnology 2(12):497-502.
ARAQUE, Y.; ALVARADO, L.; CENTENO, S.; RODRÍGUEZ, V.; VITELLI, J. 2007. Actividad antibiótica y antifúngica de B. cepacia provenientes de ambientes nosocomiales. Servicio Autónomo Hospital Universitario “Antonio Patricio Alcalá”. Cumaná, Venezuela. Kasmera. 35: 107-17.
BEVIVINO, A.; DAMALSTRI, C.; TABACCHIONI, S.; CHIARINI, L. 2000. Efficacy of Burkholderia cepacia MCL 7 in disease suppression and growth promotion of maize. Biology and Fertility of Soils, 31 (3-4): 225-231. 
BERG, G.; KRECHEL, A.; DITZ, M.; SIKORA, RA.; ULRICH, A.; HALLMANN, J. 2005. Endophytic and ectophytic potato-associated bacterial communities differ in structure and antagonistic function against plant pathogenic fungi. FEMS Microbiol Ecol 51: 215–229.
CAMPOS, R. 2011. Manejo integrado de la antracnosis en ñame. Informe final. Facultad de Ciencias Agropecuaria. Universidad de Córdoba. p38.
CERÓN, R.L.; HIGUERA, M.; B. L.; SÁNCHEZ N.J.; BUSTAMANTE, S.; BUITRAGO, G. 2006. Crecimiento y desarrollo de Colletotrichum gloeosporioides f. alatae durante su cultivo en medios líquidos. Acta Biológica Colombiana, 11 (1), 99-109. CHEN, C.; BAUSKE, E.M.; MUSSION, G.; RODRIQUEZ-KABANA, R.; KLOEPPER, JW. 1995. Biological control on Fusarium wilt on cotton by use of endophytic bacteria. Biol Control. 5: 83–91.
CHO, S.J.; PARK, S.R.; KIM, M.K; LIM, W.J.; RYU, S.K.; AN, C.L.; HONG, S.Y.; LEE, Y.H.; JEONG, S.G.; CHO, YU.; YUN, H.D. 2002. Endophytic Bacillus sp. isolated from the interior of ballon flower root. Biosci Biotechnol Biochem 66: 1270–1275.
CHO, K. M.; HONG, S. Y.; S. LEE, M.; KIM, Y. H.; KAHNG, G. G.; LIM, Y. P.; KIM, H., YUN, H. D. 2007. Endophytic Bacterial Communities in Ginseng and their Antifungal Activity Against Pathogens. Microbial Ecology. 54:341–351.
EL-BANNA N.; WINKELMANN, G. 1998. Pyrrolnitrin from Burkholderia cepacia: antibiotic activity against fungi and novel activities against Streptomycetes. J Appl Microbiol. 85:69-78.
GRANE´R, G.; PERSSON, P.; MEIJER, J., ALSTRO, M.S. 2003. A study on microbial diversity in different cultivars of Brassica napusin relation to its wilt pathogen, Verticillium longisporum. FEMS Microbiol Lett. 224: 269–276.
GLICK, B.R.; BASHAN, Y. 1997. Genetic manipulation of plant growth promoting bacteria to enhance biocontrol of phytopathogens. Biotechnol. Adv. 15:353–378.
GREEN, K. R.; ABANG, M. M.; ILOBA, C. 2000. A rapid Bioassay for Screening Yam Germplasm for Response to Anthracnose. Tropical Science. 40 (3): 132-138.
HALLMANN, J.; HALLMANN, Q.A.; MAHAFFEE, W.F.; KLOEPPER, J.W. 1997. Bacterial endophytes in agricultural crops. Can J. Microbiol. 43: 895–914.
HERNÁNDEZ, A.; GARCÍA, D.; HERNÁNDEZ, J.; HEYDRICH, M. 1997. Determinación de algunos géneros bacterianos presentes en la rizósfera del cultivo del maíz. Cultivos Tropicales, 18(3):10-14.
ISLAM, MD.; R., Y. T.; JEONG, Y.; LEE, S.; SONG, C. H. 2012. Isolation and Identification of Antifungal Compounds from Bacillus subtilis C9 Inhibiting the Growth of Plant Pathogenic Fungi. Mycobiology. 40(1):59-66.
JAYASWAL, R.; FERNÁNDEZ, M.; SOBROEDER, R. 1990. Isolation and characterization of a Pseudomonas strain that restricts growth of various phytopathogenic fungi. Applied Environmental Microbiology. 56: 1053-8.
KADIR, J.; RAHMAN, M.A.; MAHMUD, T.M.; ABDUL, R.; BEGUM, M.M. 2008. Extraction of antifungal substances from Burkholderia cepacia with antibiotic activity against Colletotrichum gloeosporioides on papaya (Carica papaya L.). Int. J. Agri. Biol., 10: 15-20.
KAI, M.; EFFMERT, U.; BERG, G.; PIECHULLA, B. 2006. Volatiles of bacterial antagonist inhibit mycelial growth of the plant pathogen Rhizoctonia solani. Appl Environ Microbiol. 55: 1053-7.
KOBAYASHI, DY.; PALUMBO, J.D. 2000. Bacterial endophytes and their effects on plants and uses in agriculture. In: Bacon, CW, White, JF, Jr (Eds.) Microbial Endophytes, Marcel Dekker, New York.
LI, X.; QUAN, C.S.; YU, H.Y.; FAN, S.D. 2008. Multiple effects of a novel compound from Burkholderia cepacia against Candida albicans. FEMS Microbiol Lett. 285: 250-6.
MAHENTHIRALINGAM, E.; T.A.; URBAN, Y. J.; GOLDBERG, B. 2005. The Multifarious, Multireplicon Burkholderia Cepacia Complex. Nature Review Microbiol. 3:144-156.
MAHAFFEE, W.F.; KLOEPPER, J.W. 1997. Temporal changes in the bacterial communities of soil, rhizosphere, and endorhiza associated with fieldgrown cucumber (Cucumis sativus L.). Microb Ecol. 34: 210–223.
MÉNDEZ, Y.; PALENCIA, J.; HERNÁNDEZ, K.; HERNÁNDEZ, E.; BELTRÁN, J. 2013. Reacción de genotipos de ñame (Dioscorea spp) a la antracnosis (Colletotrichum gloeosporioides).Temas Agrarios. 18:(1):34 - 40.
MOENNE-LOCCOZ, Y.; TICHY, H.; O’DONNEL, A.; SIMON, R.; O’GARA, F. 2001. Impact of 2, 4-Diacetylphloroglucinolproducing biocontrol strain Pseudomonas fluorescens F113 on intraspecific diversity of resident culturable fluorescent Pseudomonads associated with the roots of field-grown sugar beet seedlings. Appl Environ Microbiol 67, 3418–3425.
MUÑOZ-ROJAS, J.; FUENTES-RAMI´REZ, L.E.; CABALLERO-MELLADO, J. 2005. Antagonism among Gluconacetobacter diazotrophicus strains in culture media and in endophytic association. FEMS Microbiol Ecol. 54: 57–66
ONYEKA, T.J.; PE´TRO, D.; ANO, G.; ETIENNE, S.; RUBENS, S. 2006. Resistance in water yam (Dioscorea alata) cultivars in the French West Indies to anthracnose disease based on tissue culture-derived whole-plant assay. Plant Pathol 55, 671–678.
PALANIYAND, I. S.; YANG, S.H.; CHENG, J.H.; MENG, L.; SUH, J.-W. 2011. Biological control of anthracnose (Colletotrichum gloeosporioides) in yam by Streptomyces sp.MJM5763. Journal of Applied Microbiology 111, 443–455.
PARRA, G. E.; CENTENO, B. S.; ARAQUE, C. Y. 2009. Actividad antifúngica de Burkholderia cepacia aislada de maíz amarillo (Zea mays L.) bajo diferentes condiciones de cultivo. Revista de la Sociedad Venezolana de Microbiología; 29:103-109.
PÉREZ, A.; MARTÍNEZ, D.; BARRAZA, Z.; MARRUGO, J. 2016. Bacterias endófitas asociadas a los géneros Cyperus y Paspalum en suelos contaminados con mercurio. Rev. U.D.C.A Act. & Div. Cient. 19 (1):67-76.
QUAN, C.S.; ZHENG, W.; LIU, Q.; OHTA, Y.; FAN, SD. 2006. Isolation and characterization of a novel Burkholderia cepacia with strong antifungal activity against Rhizoctonia solani. Appl Microbiol Biotech. 72: 1276-84.
REINA, A.Y. Documentos de trabajo sobre economía regional: El cultivo del ñame en el Caribe Colombiano. Banco de la Republica, Centro de Estudios económicos Regionales-CEER. Cartagena, Colombia, p34.
SEGHERS, D.; WITTEBOLLE, L.; TOP, E.M.; VERSTRAETE, W.; SICILIANO, S.D. 2004. Impact of agricultural practice on the Zea mays L. endophytic community. Appl Environ Microbiol. 70: 1475–1482
ZINNIEL, D.K.; LAMBRECHT, P.; HARRIS, N.B.; FENG, Z.; KUCZMARSKI, D.; HIGLEY, P.; ISHMARU, C.A., ARUNAKUMARI, A.; BARLETTA, R.G.; VIDAVER, A.K. 2002. Isolation and characterization of endophytic colonizing bacteria from agronomic crops and prairie plants. Appl Environ Microbiol. 68: 2198–2208.
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dc.relation.ispartofjournal.spa.fl_str_mv Revista Colombiana de Ciencia Animal - RECIA
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spelling Doncel M, Pedro3321d049a60080f85ebd0a601a86116c300Pérez-Cordero, Alexander431437741dde2b2b001a25e6efc1b4365002017-05-05 00:00:002022-07-01T17:15:50Z2017-05-05 00:00:002022-07-01T17:15:50Z2017-05-05https://repositorio.unisucre.edu.co/handle/001/150510.24188/recia.v9.nS.2017.5182027-4297https://doi.org/10.24188/recia.v9.nS.2017.518application/pdfspaUniversidad de Sucrehttps://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2https://revistas.unisucre.edu.co/index.php/recia/article/view/518Microorganisms endophytesfungal phytopathogensmetaboliteinhibitionMicroorganismos endófitohongos fitopatógenosmetabolitoinhibición.Burkholderia cepacia aisladas de variedades de ñame con actividad antimicrobiana contra Colletotrichum gloeosporioidesBurkholderia cepacia isolated from varieties of Yam with antimicrobial activity against Colletotrichum gloeosporioidesArtículo de revistaJournal articleinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Texthttp://purl.org/redcol/resource_type/ARTREFhttp://purl.org/coar/version/c_970fb48d4fbd8a85ABANG, M.M.; WINTER, S.; MIGNOUNA, H.D.; GREEN, K.R.; ASIEDU, R. 2003). Molecular taxonomic, epidemiological and population genetic approaches to understanding yam anthracnose disease. Afr J Biotechnol 2, 486–496.ABE, M.; NAKAZAWA T. 1993. Characterization of hemolytic and antifungal substance, cepalycin, from Pseudomonas cepacia. Microbiol Inmunol. 38: 1-9.ALEMÁN, I.; SÁNCHEZ, J.; SEALEY, M.; ROJAS, J.; LÓPEZ, G. 2003. Empleo de una cepa de Burkholderia cepacia en el control de la mancha azul en la madera de pino caribe (Pinus caribaea). Ciencia. 11: 39-46. AMUSA, N.; ADEGBITE, A.; MUHAMMED, S; BAIYEWU, R. 2003. Yam disease and its management in Nigeria. African Journals of Biotecnology 2(12):497-502.ARAQUE, Y.; ALVARADO, L.; CENTENO, S.; RODRÍGUEZ, V.; VITELLI, J. 2007. Actividad antibiótica y antifúngica de B. cepacia provenientes de ambientes nosocomiales. Servicio Autónomo Hospital Universitario “Antonio Patricio Alcalá”. Cumaná, Venezuela. Kasmera. 35: 107-17.BEVIVINO, A.; DAMALSTRI, C.; TABACCHIONI, S.; CHIARINI, L. 2000. Efficacy of Burkholderia cepacia MCL 7 in disease suppression and growth promotion of maize. Biology and Fertility of Soils, 31 (3-4): 225-231. BERG, G.; KRECHEL, A.; DITZ, M.; SIKORA, RA.; ULRICH, A.; HALLMANN, J. 2005. Endophytic and ectophytic potato-associated bacterial communities differ in structure and antagonistic function against plant pathogenic fungi. FEMS Microbiol Ecol 51: 215–229.CAMPOS, R. 2011. Manejo integrado de la antracnosis en ñame. Informe final. Facultad de Ciencias Agropecuaria. Universidad de Córdoba. p38.CERÓN, R.L.; HIGUERA, M.; B. L.; SÁNCHEZ N.J.; BUSTAMANTE, S.; BUITRAGO, G. 2006. Crecimiento y desarrollo de Colletotrichum gloeosporioides f. alatae durante su cultivo en medios líquidos. Acta Biológica Colombiana, 11 (1), 99-109. CHEN, C.; BAUSKE, E.M.; MUSSION, G.; RODRIQUEZ-KABANA, R.; KLOEPPER, JW. 1995. Biological control on Fusarium wilt on cotton by use of endophytic bacteria. Biol Control. 5: 83–91.CHO, S.J.; PARK, S.R.; KIM, M.K; LIM, W.J.; RYU, S.K.; AN, C.L.; HONG, S.Y.; LEE, Y.H.; JEONG, S.G.; CHO, YU.; YUN, H.D. 2002. Endophytic Bacillus sp. isolated from the interior of ballon flower root. Biosci Biotechnol Biochem 66: 1270–1275.CHO, K. M.; HONG, S. Y.; S. LEE, M.; KIM, Y. H.; KAHNG, G. G.; LIM, Y. P.; KIM, H., YUN, H. D. 2007. Endophytic Bacterial Communities in Ginseng and their Antifungal Activity Against Pathogens. Microbial Ecology. 54:341–351.EL-BANNA N.; WINKELMANN, G. 1998. Pyrrolnitrin from Burkholderia cepacia: antibiotic activity against fungi and novel activities against Streptomycetes. J Appl Microbiol. 85:69-78.GRANE´R, G.; PERSSON, P.; MEIJER, J., ALSTRO, M.S. 2003. A study on microbial diversity in different cultivars of Brassica napusin relation to its wilt pathogen, Verticillium longisporum. FEMS Microbiol Lett. 224: 269–276.GLICK, B.R.; BASHAN, Y. 1997. Genetic manipulation of plant growth promoting bacteria to enhance biocontrol of phytopathogens. Biotechnol. Adv. 15:353–378.GREEN, K. R.; ABANG, M. M.; ILOBA, C. 2000. A rapid Bioassay for Screening Yam Germplasm for Response to Anthracnose. Tropical Science. 40 (3): 132-138.HALLMANN, J.; HALLMANN, Q.A.; MAHAFFEE, W.F.; KLOEPPER, J.W. 1997. Bacterial endophytes in agricultural crops. Can J. Microbiol. 43: 895–914.HERNÁNDEZ, A.; GARCÍA, D.; HERNÁNDEZ, J.; HEYDRICH, M. 1997. Determinación de algunos géneros bacterianos presentes en la rizósfera del cultivo del maíz. Cultivos Tropicales, 18(3):10-14.ISLAM, MD.; R., Y. T.; JEONG, Y.; LEE, S.; SONG, C. H. 2012. Isolation and Identification of Antifungal Compounds from Bacillus subtilis C9 Inhibiting the Growth of Plant Pathogenic Fungi. Mycobiology. 40(1):59-66.JAYASWAL, R.; FERNÁNDEZ, M.; SOBROEDER, R. 1990. Isolation and characterization of a Pseudomonas strain that restricts growth of various phytopathogenic fungi. Applied Environmental Microbiology. 56: 1053-8.KADIR, J.; RAHMAN, M.A.; MAHMUD, T.M.; ABDUL, R.; BEGUM, M.M. 2008. Extraction of antifungal substances from Burkholderia cepacia with antibiotic activity against Colletotrichum gloeosporioides on papaya (Carica papaya L.). Int. J. Agri. Biol., 10: 15-20.KAI, M.; EFFMERT, U.; BERG, G.; PIECHULLA, B. 2006. Volatiles of bacterial antagonist inhibit mycelial growth of the plant pathogen Rhizoctonia solani. Appl Environ Microbiol. 55: 1053-7.KOBAYASHI, DY.; PALUMBO, J.D. 2000. Bacterial endophytes and their effects on plants and uses in agriculture. In: Bacon, CW, White, JF, Jr (Eds.) Microbial Endophytes, Marcel Dekker, New York.LI, X.; QUAN, C.S.; YU, H.Y.; FAN, S.D. 2008. Multiple effects of a novel compound from Burkholderia cepacia against Candida albicans. FEMS Microbiol Lett. 285: 250-6.MAHENTHIRALINGAM, E.; T.A.; URBAN, Y. J.; GOLDBERG, B. 2005. The Multifarious, Multireplicon Burkholderia Cepacia Complex. Nature Review Microbiol. 3:144-156.MAHAFFEE, W.F.; KLOEPPER, J.W. 1997. Temporal changes in the bacterial communities of soil, rhizosphere, and endorhiza associated with fieldgrown cucumber (Cucumis sativus L.). Microb Ecol. 34: 210–223.MÉNDEZ, Y.; PALENCIA, J.; HERNÁNDEZ, K.; HERNÁNDEZ, E.; BELTRÁN, J. 2013. Reacción de genotipos de ñame (Dioscorea spp) a la antracnosis (Colletotrichum gloeosporioides).Temas Agrarios. 18:(1):34 - 40.MOENNE-LOCCOZ, Y.; TICHY, H.; O’DONNEL, A.; SIMON, R.; O’GARA, F. 2001. Impact of 2, 4-Diacetylphloroglucinolproducing biocontrol strain Pseudomonas fluorescens F113 on intraspecific diversity of resident culturable fluorescent Pseudomonads associated with the roots of field-grown sugar beet seedlings. Appl Environ Microbiol 67, 3418–3425.MUÑOZ-ROJAS, J.; FUENTES-RAMI´REZ, L.E.; CABALLERO-MELLADO, J. 2005. Antagonism among Gluconacetobacter diazotrophicus strains in culture media and in endophytic association. FEMS Microbiol Ecol. 54: 57–66ONYEKA, T.J.; PE´TRO, D.; ANO, G.; ETIENNE, S.; RUBENS, S. 2006. Resistance in water yam (Dioscorea alata) cultivars in the French West Indies to anthracnose disease based on tissue culture-derived whole-plant assay. Plant Pathol 55, 671–678.PALANIYAND, I. S.; YANG, S.H.; CHENG, J.H.; MENG, L.; SUH, J.-W. 2011. Biological control of anthracnose (Colletotrichum gloeosporioides) in yam by Streptomyces sp.MJM5763. Journal of Applied Microbiology 111, 443–455.PARRA, G. E.; CENTENO, B. S.; ARAQUE, C. Y. 2009. Actividad antifúngica de Burkholderia cepacia aislada de maíz amarillo (Zea mays L.) bajo diferentes condiciones de cultivo. Revista de la Sociedad Venezolana de Microbiología; 29:103-109.PÉREZ, A.; MARTÍNEZ, D.; BARRAZA, Z.; MARRUGO, J. 2016. Bacterias endófitas asociadas a los géneros Cyperus y Paspalum en suelos contaminados con mercurio. Rev. U.D.C.A Act. & Div. Cient. 19 (1):67-76.QUAN, C.S.; ZHENG, W.; LIU, Q.; OHTA, Y.; FAN, SD. 2006. 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