Loss of C-5 sterol desaturase activity results in increased resistance to azole and echinocandin antifungals in a clinical isolate of Candida parapsilosis.

Artículo digital.

Autores:: Rybak, Jeffrey M.
Luna Tapia, Arturo
Dickens, C. Michael
Parker, Josie E.
Caudle, Kelly E.

Tipo de recurso:: Article of journal

Fecha de publicación:: 2019

Institución:: Universidad de Sucre

Repositorio:: Repositorio Unisucre

Idioma:: eng

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dc.title.spa.fl_str_mv	Loss of C-5 sterol desaturase activity results in increased resistance to azole and echinocandin antifungals in a clinical isolate of Candida parapsilosis.
title	Loss of C-5 sterol desaturase activity results in increased resistance to azole and echinocandin antifungals in a clinical isolate of Candida parapsilosis.
spellingShingle	Loss of C-5 sterol desaturase activity results in increased resistance to azole and echinocandin antifungals in a clinical isolate of Candida parapsilosis. Candida Antifungal resistance Antimicrobial activity Antimicrobial agents Ergosterol Molecular genetics Mycology
title_short	Loss of C-5 sterol desaturase activity results in increased resistance to azole and echinocandin antifungals in a clinical isolate of Candida parapsilosis.
title_full	Loss of C-5 sterol desaturase activity results in increased resistance to azole and echinocandin antifungals in a clinical isolate of Candida parapsilosis.
title_fullStr	Loss of C-5 sterol desaturase activity results in increased resistance to azole and echinocandin antifungals in a clinical isolate of Candida parapsilosis.
title_full_unstemmed	Loss of C-5 sterol desaturase activity results in increased resistance to azole and echinocandin antifungals in a clinical isolate of Candida parapsilosis.
title_sort	Loss of C-5 sterol desaturase activity results in increased resistance to azole and echinocandin antifungals in a clinical isolate of Candida parapsilosis.
dc.creator.fl_str_mv	Rybak, Jeffrey M. Luna Tapia, Arturo Dickens, C. Michael Parker, Josie E. Caudle, Kelly E.
dc.contributor.author.spa.fl_str_mv	Rybak, Jeffrey M. Luna Tapia, Arturo Dickens, C. Michael Parker, Josie E. Caudle, Kelly E.
dc.contributor.corporatename.spa.fl_str_mv	American Society for Microbiology.
dc.subject.proposal.spa.fl_str_mv	Candida Antifungal resistance Antimicrobial activity Antimicrobial agents Ergosterol Molecular genetics Mycology
topic	Candida Antifungal resistance Antimicrobial activity Antimicrobial agents Ergosterol Molecular genetics Mycology
description	Artículo digital.
publishDate	2019
dc.date.accessioned.spa.fl_str_mv	2019-09-24T20:57:55Z
dc.date.available.spa.fl_str_mv	2019-09-24T20:57:55Z
dc.date.issued.spa.fl_str_mv	2019-09-24
dc.type.spa.fl_str_mv	Artículo de revista
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dc.identifier.citation.spa.fl_str_mv	Luna, A., Rybak, J.M., Dickens, C.M., Parker, J.E. & Caudle, K.E. (2017). Loss of C-5 Sterol Desaturase Activity Results in Increased Resistance to Azole and Echinocandin Antifungals in a Clinical Isolate of Candida parapsilosis. Antimicrobial Agents and Chemotherapy .61 (9), 651 – 657.
dc.identifier.issn.spa.fl_str_mv	1098-6596
dc.identifier.uri.spa.fl_str_mv	https://repositorio.unisucre.edu.co/handle/001/790
dc.identifier.doi.spa.fl_str_mv	https://doi.org/10.1128/AAC .00651-17
identifier_str_mv	Luna, A., Rybak, J.M., Dickens, C.M., Parker, J.E. & Caudle, K.E. (2017). Loss of C-5 Sterol Desaturase Activity Results in Increased Resistance to Azole and Echinocandin Antifungals in a Clinical Isolate of Candida parapsilosis. Antimicrobial Agents and Chemotherapy .61 (9), 651 – 657. 1098-6596
url	https://repositorio.unisucre.edu.co/handle/001/790 https://doi.org/10.1128/AAC .00651-17
dc.language.iso.spa.fl_str_mv	eng
language	eng
dc.relation.ispartof.spa.fl_str_mv	Articulo de revista
dc.relation.references.spa.fl_str_mv	Pfaller MA, Andes DR, Diekema DJ, Horn DL, Reboli AC, Rotstein C, Franks B, Azie NE. 2014. Epidemiology and outcomes of invasive candidiasis due to non-albicans species of Candida in 2,496 patients: data from the Prospective Antifungal Therapy (PATH) registry 2004-2008. PLoS One 9:e101510. https://doi.org/10.1371/journal.pone.0101510. Lockhart SR, Iqbal N, Cleveland AA, Farley MM, Harrison LH, Bolden CB, Baughman W, Stein B, Hollick R, Park BJ, Chiller T. 2012. Species identification and antifungal susceptibility testing of Candida bloodstream isolates from population-based surveillance studies in two U.S. cities from 2008 to 2011. J Clin Microbiol 50:3435–3442. https://doi.org/10 .1128/JCM.01283-12. Cleveland AA, Farley MM, Harrison LH, Stein B, Hollick R, Lockhart SR, Magill SS, Derado G, Park BJ, Chiller TM. 2012. Changes in incidence and antifungal drug resistance in candidemia: results from population-based laboratory surveillance in Atlanta and Baltimore, 2008-2011. Clin Infect Dis 55:1352–1361. https://doi.org/10.1093/cid/cis697. Trofa D, Gacser A, Nosanchuk JD. 2008. Candida parapsilosis, an emerging fungal pathogen. Clin Microbiol Rev 21:606–625. https://doi.org/10.1128/CMR.00013-08. Garcia-Effron G, Katiyar SK, Park S, Edlind TD, Perlin DS. 2008. A naturally occurring proline-to-alanine amino acid change in Fks1p in Candida parapsilosis, Candida orthopsilosis, and Candida metapsilosis accounts for reduced echinocandin susceptibility. Antimicrob Agents Chemother 52:2305–2312. https://doi.org/10.1128/AAC.00262-08.
dc.rights.spa.fl_str_mv	Derechos Reservados - Universidad de Sucre, 2019
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dc.coverage.spatial.spa.fl_str_mv	Colombia
dc.publisher.spa.fl_str_mv	Estados Unidos : Antimicrobial Agents and Chemotherapy, 2017.
dc.source.spa.fl_str_mv	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5571332/
institution	Universidad de Sucre
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spelling	Rybak, Jeffrey M.1271a4cea93c67dc07ac3a9096e56632-1Luna Tapia, Arturo37137097b30746a987e4adcda146f579-1Dickens, C. Michael49f91f6b2823516126023df923a4aeaa-1Parker, Josie E.863c9510f69a108402a5d9e704032ad8-1Caudle, Kelly E.ac73896a8b50c8bffbc441b33624fa29-1American Society for Microbiology.Colombia2019-09-24T20:57:55Z2019-09-24T20:57:55Z2019-09-24Luna, A., Rybak, J.M., Dickens, C.M., Parker, J.E. & Caudle, K.E. (2017). Loss of C-5 Sterol Desaturase Activity Results in Increased Resistance to Azole and Echinocandin Antifungals in a Clinical Isolate of Candida parapsilosis. Antimicrobial Agents and Chemotherapy .61 (9), 651 – 657.1098-6596https://repositorio.unisucre.edu.co/handle/001/790https://doi.org/10.1128/AAC .00651-17Artículo digital.Among emerging non-albicans Candida species, Candida parapsilosis is of particular concern as a cause of nosocomial bloodstream infections in neonatal and intensive care unit patients. While fluconazole and echinocandins are considered effective treatments for such infections, recent reports of fluconazole and echinocandin resistance in C. parapsilosis indicate a growing problem. The present study describes a novel mechanism of antifungal resistance in this organism affecting susceptibility to azole and echinocandin antifungals in a clinical isolate obtained from a patient with prosthetic valve endocarditis. Transcriptome analysis indicated differential expression of several genes in the resistant isolate, including upregulation of ergosterol biosynthesis pathway genes ERG2, ERG5, ERG6, ERG11, ERG24, ERG25, and UPC2.application/pdfengEstados Unidos : Antimicrobial Agents and Chemotherapy, 2017.Articulo de revistaPfaller MA, Andes DR, Diekema DJ, Horn DL, Reboli AC, Rotstein C, Franks B, Azie NE. 2014. Epidemiology and outcomes of invasive candidiasis due to non-albicans species of Candida in 2,496 patients: data from the Prospective Antifungal Therapy (PATH) registry 2004-2008. PLoS One 9:e101510. https://doi.org/10.1371/journal.pone.0101510.Lockhart SR, Iqbal N, Cleveland AA, Farley MM, Harrison LH, Bolden CB, Baughman W, Stein B, Hollick R, Park BJ, Chiller T. 2012. Species identification and antifungal susceptibility testing of Candida bloodstream isolates from population-based surveillance studies in two U.S. cities from 2008 to 2011. J Clin Microbiol 50:3435–3442. https://doi.org/10 .1128/JCM.01283-12.Cleveland AA, Farley MM, Harrison LH, Stein B, Hollick R, Lockhart SR, Magill SS, Derado G, Park BJ, Chiller TM. 2012. Changes in incidence and antifungal drug resistance in candidemia: results from population-based laboratory surveillance in Atlanta and Baltimore, 2008-2011. Clin Infect Dis 55:1352–1361. https://doi.org/10.1093/cid/cis697.Trofa D, Gacser A, Nosanchuk JD. 2008. Candida parapsilosis, an emerging fungal pathogen. Clin Microbiol Rev 21:606–625. https://doi.org/10.1128/CMR.00013-08.Garcia-Effron G, Katiyar SK, Park S, Edlind TD, Perlin DS. 2008. A naturally occurring proline-to-alanine amino acid change in Fks1p in Candida parapsilosis, Candida orthopsilosis, and Candida metapsilosis accounts for reduced echinocandin susceptibility. Antimicrob Agents Chemother 52:2305–2312. https://doi.org/10.1128/AAC.00262-08.Derechos Reservados - Universidad de Sucre, 2019https://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)http://purl.org/coar/access_right/c_abf2https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5571332/Loss of C-5 sterol desaturase activity results in increased resistance to azole and echinocandin antifungals in a clinical isolate of Candida parapsilosis.Artículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501Texthttp://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85CandidaAntifungal resistanceAntimicrobial activityAntimicrobial agentsErgosterolMolecular geneticsMycologyPublicationORIGINALLoss of C-5 sterol desaturase activity results in increased resistance to azole and echinocandin antifungals in a clinical isolate of Candida parapsilosis..pdfLoss of C-5 sterol desaturase activity results in increased resistance to azole and echinocandin antifungals in a clinical isolate of Candida parapsilosis..pdfapplication/pdf1707457https://repositorio.unisucre.edu.co/bitstreams/976c463f-402f-4f02-8a1b-5a1ce3f828ea/downloadd168880d01869b7f6adaac4191edf0eeMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81366https://repositorio.unisucre.edu.co/bitstreams/34938aeb-3cf9-4f1a-8496-a64e71460fa4/download5f839364c91422e4b2a78812717048fbMD52TEXTLoss of C-5 sterol desaturase activity results in increased resistance to azole and echinocandin antifungals in a clinical isolate of Candida parapsilosis..pdf.txtLoss of C-5 sterol desaturase activity results in increased resistance to azole and echinocandin antifungals in a clinical isolate of Candida parapsilosis..pdf.txtExtracted texttext/plain51463https://repositorio.unisucre.edu.co/bitstreams/c3992913-7ed1-46e3-88da-9efe1d0a5e94/downloada89c0bef434d041a8cc7fd30378dbdf9MD55THUMBNAILLoss of C-5 sterol desaturase activity results in increased resistance to azole and echinocandin antifungals in a clinical isolate of Candida parapsilosis..pdf.jpgLoss of C-5 sterol desaturase activity results in increased resistance to azole and echinocandin antifungals in a clinical isolate of Candida parapsilosis..pdf.jpgGenerated Thumbnailimage/jpeg14608https://repositorio.unisucre.edu.co/bitstreams/8bdde73c-737f-4c20-9f41-4658f185a88f/download6256b6cd842ce645a114e6ede8823385MD56001/790oai:repositorio.unisucre.edu.co:001/7902024-04-17 16:30:40.614https://creativecommons.org/licenses/by-nc/4.0/Derechos Reservados - Universidad de Sucre, 2019open.accesshttps://repositorio.unisucre.edu.coRepositorio Institucional Universidad de Sucrebdigital@metabiblioteca.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

Loss of C-5 sterol desaturase activity results in increased resistance to azole and echinocandin antifungals in a clinical isolate of Candida parapsilosis.

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