ERG2 and ERG24 are required for normal vacuolar physiology as well as candida albicans pathogenicity in a murine model of disseminated but not vaginal candidiasis.

Artículo digital.

Autores:: Luna Tapia, Arturo
Peters, Brian M.
Eberle, Karen E.
Kerns, Morgan E.
Palmer, Glen E.
Foster, Timothy P.
Marrero, Luis
Noverr, Mairi C.
Fidel , Paul L.

Tipo de recurso:: Article of journal

Fecha de publicación:: 2019

Institución:: Universidad de Sucre

Repositorio:: Repositorio Unisucre

Idioma:: eng

id	RUNISUCRE2_9b634a4a5c07726f09623c16ed87c3a9
oai_identifier_str	oai:repositorio.unisucre.edu.co:001/813
network_acronym_str	RUNISUCRE2
network_name_str	Repositorio Unisucre
repository_id_str
dc.title.spa.fl_str_mv	ERG2 and ERG24 are required for normal vacuolar physiology as well as candida albicans pathogenicity in a murine model of disseminated but not vaginal candidiasis.
title	ERG2 and ERG24 are required for normal vacuolar physiology as well as candida albicans pathogenicity in a murine model of disseminated but not vaginal candidiasis.
spellingShingle	ERG2 and ERG24 are required for normal vacuolar physiology as well as candida albicans pathogenicity in a murine model of disseminated but not vaginal candidiasis. Candida albicans ERG2 ERG24 Pathogenicity
title_short	ERG2 and ERG24 are required for normal vacuolar physiology as well as candida albicans pathogenicity in a murine model of disseminated but not vaginal candidiasis.
title_full	ERG2 and ERG24 are required for normal vacuolar physiology as well as candida albicans pathogenicity in a murine model of disseminated but not vaginal candidiasis.
title_fullStr	ERG2 and ERG24 are required for normal vacuolar physiology as well as candida albicans pathogenicity in a murine model of disseminated but not vaginal candidiasis.
title_full_unstemmed	ERG2 and ERG24 are required for normal vacuolar physiology as well as candida albicans pathogenicity in a murine model of disseminated but not vaginal candidiasis.
title_sort	ERG2 and ERG24 are required for normal vacuolar physiology as well as candida albicans pathogenicity in a murine model of disseminated but not vaginal candidiasis.
dc.creator.fl_str_mv	Luna Tapia, Arturo Peters, Brian M. Eberle, Karen E. Kerns, Morgan E. Palmer, Glen E. Foster, Timothy P. Marrero, Luis Noverr, Mairi C. Fidel , Paul L.
dc.contributor.author.spa.fl_str_mv	Luna Tapia, Arturo Peters, Brian M. Eberle, Karen E. Kerns, Morgan E. Palmer, Glen E. Foster, Timothy P. Marrero, Luis Noverr, Mairi C. Fidel , Paul L.
dc.contributor.corporatename.spa.fl_str_mv	American Society for Microbiology.
dc.subject.proposal.spa.fl_str_mv	Candida albicans ERG2 ERG24 Pathogenicity
topic	Candida albicans ERG2 ERG24 Pathogenicity
description	Artículo digital.
publishDate	2019
dc.date.accessioned.spa.fl_str_mv	2019-10-01T21:23:15Z
dc.date.available.spa.fl_str_mv	2019-10-01T21:23:15Z
dc.date.issued.spa.fl_str_mv	2019-10-01
dc.type.spa.fl_str_mv	Artículo de revista
dc.type.coarversion.fl_str_mv	http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/article
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.coar.spa.fl_str_mv	http://purl.org/coar/resource_type/c_6501
dc.type.content.spa.fl_str_mv	Text
dc.type.redcol.spa.fl_str_mv	http://purl.org/coar/resource_type/c_2df8fbb1
format	http://purl.org/coar/resource_type/c_6501
status_str	publishedVersion
dc.identifier.citation.spa.fl_str_mv	Luna, A., Peters, B.M., Eberle, K.E., Kerns, M.E., Foster, T.P., Marrero, L., Noverr, M.C., Fidel, P.L. & Palmer, G.E. (2015). ERG2 and ERG24 are required for normal vacuolar physiology as well as candida albicans pathogenicity in a murine model of disseminated but not vaginal candidiasis. Eukaryotic Cell. 14(10), 1006 – 1016.
dc.identifier.issn.spa.fl_str_mv	1535-9786
dc.identifier.uri.spa.fl_str_mv	https://repositorio.unisucre.edu.co/handle/001/813
dc.identifier.doi.spa.fl_str_mv	10.1128/EC.00116-15
identifier_str_mv	Luna, A., Peters, B.M., Eberle, K.E., Kerns, M.E., Foster, T.P., Marrero, L., Noverr, M.C., Fidel, P.L. & Palmer, G.E. (2015). ERG2 and ERG24 are required for normal vacuolar physiology as well as candida albicans pathogenicity in a murine model of disseminated but not vaginal candidiasis. Eukaryotic Cell. 14(10), 1006 – 1016. 1535-9786 10.1128/EC.00116-15
url	https://repositorio.unisucre.edu.co/handle/001/813
dc.language.iso.spa.fl_str_mv	eng
language	eng
dc.relation.ispartof.spa.fl_str_mv	Articulo de revista
dc.relation.references.spa.fl_str_mv	Odds FC, Brown AJ, Gow NA. 2003. Antifungal agents: mechanisms of action. Trends Microbiol 11:272–279. http://dx.doi.org/10.1016/S0966-842X(03)00117-3. Kelly SL, Lamb DC, Corran AJ, Baldwin BC, Kelly DE. 1995. Mode of action and resistance to azole antifungals associated with the formation of 14 alpha-methylergosta-8,24(28)-dien-3beta,6alpha-diol. Biochem Biophys Res Commun 207:910–915. http://dx.doi.org/10.1006/bbrc.1995.1272. Luna-Tapia A, Kerns ME, Eberle KE, Jursic BS, Palmer GE. 2015. Trafficking through the late endosome significantly impacts Candida albicans tolerance of the azole antifungals. Antimicrob Agents Chemother 59:2410–2420. http://dx.doi.org/10.1128/AAC.04239-14. Heese-Peck A, Pichler H, Zanolari B, Watanabe R, Daum G, Riezman H. 2002. Multiple functions of sterols in yeast endocytosis. Mol Biol Cell 13:2664–2680. http://dx.doi.org/10.1091/mbc.E02-04-0186. Kato M, Wickner WT. 2001. Ergosterol is required for the Sec18/ATPdependent priming step of homotypic vacuole fusion. EMBO J 20:4035– 4040. http://dx.doi.org/10.1093/emboj/20.15.4035.
dc.rights.spa.fl_str_mv	Derechos Reservados - Universidad de Sucre, 2019
dc.rights.coar.fl_str_mv	http://purl.org/coar/access_right/c_abf2
dc.rights.uri.spa.fl_str_mv	https://creativecommons.org/licenses/by-nc/4.0/
dc.rights.accessrights.spa.fl_str_mv	info:eu-repo/semantics/openAccess
dc.rights.creativecommons.spa.fl_str_mv	Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)
rights_invalid_str_mv	Derechos Reservados - Universidad de Sucre, 2019 https://creativecommons.org/licenses/by-nc/4.0/ Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv	openAccess
dc.format.mimetype.spa.fl_str_mv	application/pdf
dc.coverage.spatial.spa.fl_str_mv	Colombia
dc.publisher.spa.fl_str_mv	Estados Unidos: Eukaryotic Cell, 2015.
dc.source.spa.fl_str_mv	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588627
institution	Universidad de Sucre
bitstream.url.fl_str_mv	https://repositorio.unisucre.edu.co/bitstreams/bf83d47b-5a56-4a41-b097-b79f796f1e40/download https://repositorio.unisucre.edu.co/bitstreams/4667eb62-4b66-4a87-9adb-341f37b0ba9d/download https://repositorio.unisucre.edu.co/bitstreams/a55c5707-51e6-4761-8dc0-08a4edc65783/download https://repositorio.unisucre.edu.co/bitstreams/5f0a5a33-9b68-4b5a-99da-b555b12f46af/download
bitstream.checksum.fl_str_mv	f2444ae5243d6b6b77380f70bf64323a 2b9e74cf2d384296aaf78d54c188ec32 bb632769ec79924dbc6382b52ea897f3 5f839364c91422e4b2a78812717048fb
bitstream.checksumAlgorithm.fl_str_mv	MD5 MD5 MD5 MD5
repository.name.fl_str_mv	Repositorio Institucional Universidad de Sucre
repository.mail.fl_str_mv	bdigital@metabiblioteca.com
_version_	1818102611474120704
spelling	Luna Tapia, Arturo37137097b30746a987e4adcda146f579-1Peters, Brian M.6950b411f4c62c3a9b335f472f53acab-1Eberle, Karen E.81a867ef93ce49f01c8b04446c8a8a05-1Kerns, Morgan E.f0f22b99ef79a8e4c884a6bf345626e8-1Palmer, Glen E.5f5dd8dc79907c8395ffb4851dee7394-1Foster, Timothy P.10ebda7de3da8bc828a78621fd81d389-1Marrero, Luise83afad538d00b2c24c4105dce91ba56-1Noverr, Mairi C.5cd357df7ecce61e9c66b2af08851d84-1Fidel , Paul L.216603284c7ab022864eb94079b98d79-1American Society for Microbiology.Colombia2019-10-01T21:23:15Z2019-10-01T21:23:15Z2019-10-01Luna, A., Peters, B.M., Eberle, K.E., Kerns, M.E., Foster, T.P., Marrero, L., Noverr, M.C., Fidel, P.L. & Palmer, G.E. (2015). ERG2 and ERG24 are required for normal vacuolar physiology as well as candida albicans pathogenicity in a murine model of disseminated but not vaginal candidiasis. Eukaryotic Cell. 14(10), 1006 – 1016.1535-9786https://repositorio.unisucre.edu.co/handle/001/81310.1128/EC.00116-15Artículo digital.Several important classes of antifungal agents, including the azoles, act by blocking ergosterol biosynthesis. It was recently reported that the azoles cause massive disruption of the fungal vacuole in the prevalent human pathogen Candida albicans. This is significant because normal vacuolar function is required to support C. albicans pathogenicity. This study examined the impact of the morpholine antifungals, which inhibit later steps of ergosterol biosynthesis, on C. albicans vacuolar integrity. It was found that overexpression of either the ERG2 or ERG24 gene, encoding C-8 sterol isomerase or C-14 sterol reductase, respectively, suppressed C. albicans sensitivity to the morpholines. In addition, both erg2 / and erg24 / mutants were hypersensitive to the morpholines. These data are consistent with the antifungal activity of the morpholines depending upon the simultaneous inhibition of both Erg2p and Erg24p. The vacuoles within both erg2 / and erg24 / C. albicans strains exhibited an aberrant morphology and accumulated large quantities of the weak base quinacrine, indicating enhanced vacuolar acidification compared with that of control strains. Both erg mutants exhibited significant defects in polarized hyphal growth and were avirulent in a mouse model of disseminated candidiasis. Surprisingly, in a mouse model of vaginal candidiasis, both mutants colonized mice at high levels and induced a pathogenic response similar to that with the controls. Thus, while targeting Erg2p or Erg24p alone could provide a potentially efficacious therapy for disseminated candidiasis, it may not be an effective strategy to treat vaginal infections. The potential value of drugs targeting these enzymes as adjunctive therapies is discussed.application/pdfengEstados Unidos: Eukaryotic Cell, 2015.Articulo de revistaOdds FC, Brown AJ, Gow NA. 2003. Antifungal agents: mechanisms of action. Trends Microbiol 11:272–279. http://dx.doi.org/10.1016/S0966-842X(03)00117-3.Kelly SL, Lamb DC, Corran AJ, Baldwin BC, Kelly DE. 1995. Mode of action and resistance to azole antifungals associated with the formation of 14 alpha-methylergosta-8,24(28)-dien-3beta,6alpha-diol. Biochem Biophys Res Commun 207:910–915. http://dx.doi.org/10.1006/bbrc.1995.1272.Luna-Tapia A, Kerns ME, Eberle KE, Jursic BS, Palmer GE. 2015. Trafficking through the late endosome significantly impacts Candida albicans tolerance of the azole antifungals. Antimicrob Agents Chemother 59:2410–2420. http://dx.doi.org/10.1128/AAC.04239-14.Heese-Peck A, Pichler H, Zanolari B, Watanabe R, Daum G, Riezman H. 2002. Multiple functions of sterols in yeast endocytosis. Mol Biol Cell 13:2664–2680. http://dx.doi.org/10.1091/mbc.E02-04-0186.Kato M, Wickner WT. 2001. Ergosterol is required for the Sec18/ATPdependent priming step of homotypic vacuole fusion. EMBO J 20:4035– 4040. http://dx.doi.org/10.1093/emboj/20.15.4035.Derechos Reservados - Universidad de Sucre, 2019https://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)http://purl.org/coar/access_right/c_abf2https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588627ERG2 and ERG24 are required for normal vacuolar physiology as well as candida albicans pathogenicity in a murine model of disseminated but not vaginal candidiasis.Artículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501Texthttp://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Candida albicansERG2ERG24PathogenicityPublicationTEXTERG2 and ERG24 Are Required for Normal Vacuolar Physiology as Well as Candida albicans Pathogenicity in a Murine Model of Disseminated but Not Vaginal Candidiasis..pdf.txtERG2 and ERG24 Are Required for Normal Vacuolar Physiology as Well as Candida albicans Pathogenicity in a Murine Model of Disseminated but Not Vaginal Candidiasis..pdf.txtExtracted texttext/plain68408https://repositorio.unisucre.edu.co/bitstreams/bf83d47b-5a56-4a41-b097-b79f796f1e40/downloadf2444ae5243d6b6b77380f70bf64323aMD55THUMBNAILERG2 and ERG24 Are Required for Normal Vacuolar Physiology as Well as Candida albicans Pathogenicity in a Murine Model of Disseminated but Not Vaginal Candidiasis..pdf.jpgERG2 and ERG24 Are Required for Normal Vacuolar Physiology as Well as Candida albicans Pathogenicity in a Murine Model of Disseminated but Not Vaginal Candidiasis..pdf.jpgGenerated Thumbnailimage/jpeg16724https://repositorio.unisucre.edu.co/bitstreams/4667eb62-4b66-4a87-9adb-341f37b0ba9d/download2b9e74cf2d384296aaf78d54c188ec32MD56ORIGINALERG2 and ERG24 Are Required for Normal Vacuolar Physiology as Well as Candida albicans Pathogenicity in a Murine Model of Disseminated but Not Vaginal Candidiasis..pdfERG2 and ERG24 Are Required for Normal Vacuolar Physiology as Well as Candida albicans Pathogenicity in a Murine Model of Disseminated but Not Vaginal Candidiasis..pdfapplication/pdf1362096https://repositorio.unisucre.edu.co/bitstreams/a55c5707-51e6-4761-8dc0-08a4edc65783/downloadbb632769ec79924dbc6382b52ea897f3MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81366https://repositorio.unisucre.edu.co/bitstreams/5f0a5a33-9b68-4b5a-99da-b555b12f46af/download5f839364c91422e4b2a78812717048fbMD52001/813oai:repositorio.unisucre.edu.co:001/8132024-12-05 09:51:50.436https://creativecommons.org/licenses/by-nc/4.0/Derechos Reservados - Universidad de Sucre, 2019open.accesshttps://repositorio.unisucre.edu.coRepositorio Institucional Universidad de Sucrebdigital@metabiblioteca.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

ERG2 and ERG24 are required for normal vacuolar physiology as well as candida albicans pathogenicity in a murine model of disseminated but not vaginal candidiasis.

Publicaciones similares