Molecular Evidence Confirms the Taxonomic Separation of Lutzomyia tihuiliensis from Lutzomyia pia (Diptera: Psychodidae) and the Usefulness of Pleural Pigmentation Patterns in Species Identification.

Artículo digital.

Autores:: Pérez Doria, Alveiro
Bejarano, Eduar Elías
Sierra, Diana
Vélez, Iván Darío

Tipo de recurso:: Article of journal

Fecha de publicación:: 2019

Institución:: Universidad de Sucre

Repositorio:: Repositorio Unisucre

Idioma:: eng

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dc.title.spa.fl_str_mv	Molecular Evidence Confirms the Taxonomic Separation of Lutzomyia tihuiliensis from Lutzomyia pia (Diptera: Psychodidae) and the Usefulness of Pleural Pigmentation Patterns in Species Identification.
title	Molecular Evidence Confirms the Taxonomic Separation of Lutzomyia tihuiliensis from Lutzomyia pia (Diptera: Psychodidae) and the Usefulness of Pleural Pigmentation Patterns in Species Identification.
spellingShingle	Molecular Evidence Confirms the Taxonomic Separation of Lutzomyia tihuiliensis from Lutzomyia pia (Diptera: Psychodidae) and the Usefulness of Pleural Pigmentation Patterns in Species Identification. sand flies Lutzomyia verrucarum group Lutzomyia pia Lutzomyia tihuiliensis Cytochrome b
title_short	Molecular Evidence Confirms the Taxonomic Separation of Lutzomyia tihuiliensis from Lutzomyia pia (Diptera: Psychodidae) and the Usefulness of Pleural Pigmentation Patterns in Species Identification.
title_full	Molecular Evidence Confirms the Taxonomic Separation of Lutzomyia tihuiliensis from Lutzomyia pia (Diptera: Psychodidae) and the Usefulness of Pleural Pigmentation Patterns in Species Identification.
title_fullStr	Molecular Evidence Confirms the Taxonomic Separation of Lutzomyia tihuiliensis from Lutzomyia pia (Diptera: Psychodidae) and the Usefulness of Pleural Pigmentation Patterns in Species Identification.
title_full_unstemmed	Molecular Evidence Confirms the Taxonomic Separation of Lutzomyia tihuiliensis from Lutzomyia pia (Diptera: Psychodidae) and the Usefulness of Pleural Pigmentation Patterns in Species Identification.
title_sort	Molecular Evidence Confirms the Taxonomic Separation of Lutzomyia tihuiliensis from Lutzomyia pia (Diptera: Psychodidae) and the Usefulness of Pleural Pigmentation Patterns in Species Identification.
dc.creator.fl_str_mv	Pérez Doria, Alveiro Bejarano, Eduar Elías Sierra, Diana Vélez, Iván Darío
dc.contributor.author.spa.fl_str_mv	Pérez Doria, Alveiro Bejarano, Eduar Elías Sierra, Diana Vélez, Iván Darío
dc.contributor.corporatename.spa.fl_str_mv	Entomological Society of America.
dc.subject.proposal.spa.fl_str_mv	sand flies Lutzomyia verrucarum group Lutzomyia pia Lutzomyia tihuiliensis Cytochrome b
topic	sand flies Lutzomyia verrucarum group Lutzomyia pia Lutzomyia tihuiliensis Cytochrome b
description	Artículo digital.
publishDate	2019
dc.date.accessioned.spa.fl_str_mv	2019-11-08T18:31:28Z
dc.date.available.spa.fl_str_mv	2019-11-08T18:31:28Z
dc.date.issued.spa.fl_str_mv	2019-11-08
dc.type.spa.fl_str_mv	Artículo de revista
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dc.identifier.citation.spa.fl_str_mv	Pérez, A. J., Bejarano, E.E., Sierra, D. & Vélez, I.D. (2008). Molecular Evidence Confirms the Taxonomic Separation of Lutzomyia tihuiliensis from Lutzomyia pia (Diptera: Psychodidae) and the Usefulness of Pleural Pigmentation Patterns in Species Identification. Journal of Medical Entomology. 45(4): 653-659.
dc.identifier.issn.spa.fl_str_mv	0022-2585.
dc.identifier.uri.spa.fl_str_mv	https://repositorio.unisucre.edu.co/handle/001/965
identifier_str_mv	Pérez, A. J., Bejarano, E.E., Sierra, D. & Vélez, I.D. (2008). Molecular Evidence Confirms the Taxonomic Separation of Lutzomyia tihuiliensis from Lutzomyia pia (Diptera: Psychodidae) and the Usefulness of Pleural Pigmentation Patterns in Species Identification. Journal of Medical Entomology. 45(4): 653-659. 0022-2585.
url	https://repositorio.unisucre.edu.co/handle/001/965
dc.language.iso.spa.fl_str_mv	eng
language	eng
dc.relation.ispartof.spa.fl_str_mv	Articulo de revista
dc.relation.references.spa.fl_str_mv	Agudelo, L. A., J. Uribe, D. Sierra, F. Ruiz, and I. D. Ve´lez. 2002. Presence of American cutaneous leishmaniasis vectors surrounding the city of Medellõ´n, Colombia. Mem. Inst. Oswaldo Cruz 97: 641Ð 642. Alexander, B., C. Ferro, D. G. Young, A. Morales, and R. B. Tesh. 1992. Ecology of phlebotomine sand ßies (Diptera: Psychodidae) in a focus of Leishmania (Viannia) braziliensis in northeastern Colombia. Mem. Inst. Oswaldo Cruz 87: 387Ð395. Altschul, S. F., W. Gish, W. Miller, E. W. Myers, and D. J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215: 403Ð 410. Arrivillaga, J. C., D. E. Norris, M. D. Feliciangeli, and G. C. Lanzaro. 2002. Phylogeography of the Neotropical sand ßy Lutzomyia longipalpis inferred from mitochondrial DNA sequences. Infect. Genet. Evol. 2: 83Ð95.Arrivillaga, J. C., D. E. Norris, M. D. Feliciangeli, and G. C. Lanzaro. 2002. Phylogeography of the Neotropical sand ßy Lutzomyia longipalpis inferred from mitochondrial DNA sequences. Infect. Genet. Evol. 2: 83Ð95. Beard, C. B., D. M. Hamm, and F. H. Collins. 1993. The mitochondrial genome of the mosquito Anopheles gambiae: DNA sequence, genome organization, and comparisons with mitochondrial sequences of other insects. Insect Mol. Biol. 2: 103Ð124.
dc.rights.spa.fl_str_mv	Derechos Reservados - Universidad de Sucre, 2019
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dc.rights.creativecommons.spa.fl_str_mv	Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)
rights_invalid_str_mv	Derechos Reservados - Universidad de Sucre, 2019 https://creativecommons.org/licenses/by-nc/4.0/ Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv	openAccess
dc.format.mimetype.spa.fl_str_mv	application/pdf
dc.coverage.spatial.spa.fl_str_mv	Colombia
dc.publisher.spa.fl_str_mv	Estados Unidos: Journal of Medical Entomology, 2008.
dc.source.spa.fl_str_mv	https://www.researchgate.net/publication/23182085.
institution	Universidad de Sucre
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spelling	Pérez Doria, Alveiro0d3ce4ee44272be00e95735466d41519-1Bejarano, Eduar Elíasbdf57c514088820492acdc61e67d7db9-1Sierra, Diana073eb4ebd764f3fd54392ceebf300ed3-1Vélez, Iván Darío7b74562987acbb80c0b85b4e7a416d42-1Entomological Society of America.Colombia2019-11-08T18:31:28Z2019-11-08T18:31:28Z2019-11-08Pérez, A. J., Bejarano, E.E., Sierra, D. & Vélez, I.D. (2008). Molecular Evidence Confirms the Taxonomic Separation of Lutzomyia tihuiliensis from Lutzomyia pia (Diptera: Psychodidae) and the Usefulness of Pleural Pigmentation Patterns in Species Identification. Journal of Medical Entomology. 45(4): 653-659.0022-2585.https://repositorio.unisucre.edu.co/handle/001/965Artículo digital.The phlebotomine sand flies Lutzomyia pia (Fairchild & Hertig 1961) and Lutzomyia tihuiliensis Le Pont, Torrez-Espejo & Dujardin 1997 (Diptera: Psychodidae) belong to the pia series of the Lu. verrucarum species group, which includes several species that bite humans in Andean foci of leishmaniasis. The females of these two species exhibit isometry and isomorphism in anatomical structures of the head and terminalia commonly used in taxonomic identipcation of sand bies. They can only be differentiated based on subtle differences in the pigmentation of the pleura. In Lu. tihuiliensis, this is restricted to the basal portions of the katepimeron and katepisternum, whereas in Lu. piaboth structures are totally pigmented. Takinginto account the subtle morphological differences between these species, the objective of the current study was to evaluate the specipc taxonomic status of Lu. tihuiliensis with respect to Lu. pia. A 475-bp portion of the mitochondrial genome was sequenced, composed of the 3 end of the cytochrome b gene, intergenic spacer 1, the transfer RNA gene for serine, intergenic spacer 2, and the 3 end of the gene NAD dehydrogenase 1. Genetic analysis conÞrms that Lu. tihuiliensis and Lu. pia constitute two distinct species and this is supported by four strong lines of evidence, i.e., the paired genetic distances, size differences and amino acid composition of the cytochrome b protein, presence and absence of intergenic spacer one and divergence observed in the sequence of the transfer RNA gene for serine. It also conÞrms the validity of the pleural pigmentation pattern as a species diagnostic character and the importance of performing a detailed examination of this character during morphological determination of phlebotomine sand flies in the series pia.application/pdfengEstados Unidos: Journal of Medical Entomology, 2008.Articulo de revistaAgudelo, L. A., J. Uribe, D. Sierra, F. Ruiz, and I. D. Ve´lez. 2002. Presence of American cutaneous leishmaniasis vectors surrounding the city of Medellõ´n, Colombia. Mem. Inst. Oswaldo Cruz 97: 641Ð 642.Alexander, B., C. Ferro, D. G. Young, A. Morales, and R. B. Tesh. 1992. Ecology of phlebotomine sand ßies (Diptera: Psychodidae) in a focus of Leishmania (Viannia) braziliensis in northeastern Colombia. Mem. Inst. Oswaldo Cruz 87: 387Ð395.Altschul, S. F., W. Gish, W. Miller, E. W. Myers, and D. J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215: 403Ð 410.Arrivillaga, J. C., D. E. Norris, M. D. Feliciangeli, and G. C. Lanzaro. 2002. Phylogeography of the Neotropical sand ßy Lutzomyia longipalpis inferred from mitochondrial DNA sequences. Infect. Genet. Evol. 2: 83Ð95.Arrivillaga, J. C., D. E. Norris, M. D. Feliciangeli, and G. C. Lanzaro. 2002. Phylogeography of the Neotropical sand ßy Lutzomyia longipalpis inferred from mitochondrial DNA sequences. Infect. Genet. Evol. 2: 83Ð95.Beard, C. B., D. M. Hamm, and F. H. Collins. 1993. The mitochondrial genome of the mosquito Anopheles gambiae: DNA sequence, genome organization, and comparisons with mitochondrial sequences of other insects. Insect Mol. Biol. 2: 103Ð124.Derechos Reservados - Universidad de Sucre, 2019https://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)http://purl.org/coar/access_right/c_abf2https://www.researchgate.net/publication/23182085.Molecular Evidence Confirms the Taxonomic Separation of Lutzomyia tihuiliensis from Lutzomyia pia (Diptera: Psychodidae) and the Usefulness of Pleural Pigmentation Patterns in Species Identification.Artículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501Texthttp://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85sand fliesLutzomyia verrucarum groupLutzomyia piaLutzomyia tihuiliensisCytochrome bPublicationORIGINALMolecular Evidence Confirms the Taxonomic Separation of Lutzomyia tihuiliensis from Lutzomyia pia (Diptera Psychodidae) and the Usefulness of Pleural Pigmentation Patterns in Species Identification..pdfMolecular Evidence Confirms the Taxonomic Separation of Lutzomyia tihuiliensis from Lutzomyia pia (Diptera Psychodidae) and the Usefulness of Pleural Pigmentation Patterns in Species Identification..pdfapplication/pdf1004753https://repositorio.unisucre.edu.co/bitstreams/60544209-22fb-4d03-a415-c15566f3421d/downloade9f8c306799093793d165c7be91b284bMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81366https://repositorio.unisucre.edu.co/bitstreams/ee85e4f6-5f8e-4d06-a259-6fcd9412bc50/download5f839364c91422e4b2a78812717048fbMD52TEXTMolecular Evidence Confirms the Taxonomic Separation of Lutzomyia tihuiliensis from Lutzomyia pia (Diptera Psychodidae) and the Usefulness of Pleural Pigmentation Patterns in Species Identification..pdf.txtMolecular Evidence Confirms the Taxonomic Separation of Lutzomyia tihuiliensis from Lutzomyia pia (Diptera Psychodidae) and the Usefulness of Pleural Pigmentation Patterns in Species Identification..pdf.txtExtracted texttext/plain29082https://repositorio.unisucre.edu.co/bitstreams/23b52275-98e3-4bd6-b883-44af9aeada89/download688daffa39fd8bb94652513d3a2b3849MD55THUMBNAILMolecular Evidence Confirms the Taxonomic Separation of Lutzomyia tihuiliensis from Lutzomyia pia (Diptera Psychodidae) and the Usefulness of Pleural Pigmentation Patterns in Species Identification..pdf.jpgMolecular Evidence Confirms the Taxonomic Separation of Lutzomyia tihuiliensis from Lutzomyia pia (Diptera Psychodidae) and the Usefulness of Pleural Pigmentation Patterns in Species Identification..pdf.jpgGenerated Thumbnailimage/jpeg14938https://repositorio.unisucre.edu.co/bitstreams/1fca7be7-4c4d-4099-bcc6-615ee5e579b3/downloadf3098417ddc30d9fab8f521008f685e1MD56001/965oai:repositorio.unisucre.edu.co:001/9652024-04-17 16:30:12.273https://creativecommons.org/licenses/by-nc/4.0/Derechos Reservados - Universidad de Sucre, 2019open.accesshttps://repositorio.unisucre.edu.coRepositorio Institucional Universidad de Sucrebdigital@metabiblioteca.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

Molecular Evidence Confirms the Taxonomic Separation of Lutzomyia tihuiliensis from Lutzomyia pia (Diptera: Psychodidae) and the Usefulness of Pleural Pigmentation Patterns in Species Identification.

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