Diagnostic accuracy of rKLO8 versus rK26 ELISAs for screening of canine visceral leishmaniasis.
Artículo digital.
- Autores:
-
Martínez Abad, Lily P.
Almeida, Caroline S.
M. Mattos, Ana Márcia
Mendonça, Ana Carolina
M. Alves, Márcio J.
Pinheiro, Aimara C.
Porrozzi, Renato
Abass, Elfadil
Ulrich, Steinhoff
Teixeira, Henrique C.
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2019
- Institución:
- Universidad de Sucre
- Repositorio:
- Repositorio Unisucre
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.unisucre.edu.co:001/955
- Acceso en línea:
- https://repositorio.unisucre.edu.co/handle/001/955
http://dx.doi.org/doi:10.1016/j.actatropica.2016.11.021.
- Palabra clave:
- Canine visceral leishmaniasis
Serodiagnosis
rKLO8
rK26
- Rights
- openAccess
- License
- Derechos Reservados - Universidad de Sucre, 2019
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| dc.title.spa.fl_str_mv |
Diagnostic accuracy of rKLO8 versus rK26 ELISAs for screening of canine visceral leishmaniasis. |
| title |
Diagnostic accuracy of rKLO8 versus rK26 ELISAs for screening of canine visceral leishmaniasis. |
| spellingShingle |
Diagnostic accuracy of rKLO8 versus rK26 ELISAs for screening of canine visceral leishmaniasis. Canine visceral leishmaniasis Serodiagnosis rKLO8 rK26 |
| title_short |
Diagnostic accuracy of rKLO8 versus rK26 ELISAs for screening of canine visceral leishmaniasis. |
| title_full |
Diagnostic accuracy of rKLO8 versus rK26 ELISAs for screening of canine visceral leishmaniasis. |
| title_fullStr |
Diagnostic accuracy of rKLO8 versus rK26 ELISAs for screening of canine visceral leishmaniasis. |
| title_full_unstemmed |
Diagnostic accuracy of rKLO8 versus rK26 ELISAs for screening of canine visceral leishmaniasis. |
| title_sort |
Diagnostic accuracy of rKLO8 versus rK26 ELISAs for screening of canine visceral leishmaniasis. |
| dc.creator.fl_str_mv |
Martínez Abad, Lily P. Almeida, Caroline S. M. Mattos, Ana Márcia Mendonça, Ana Carolina M. Alves, Márcio J. Pinheiro, Aimara C. Porrozzi, Renato Abass, Elfadil Ulrich, Steinhoff Teixeira, Henrique C. |
| dc.contributor.author.spa.fl_str_mv |
Martínez Abad, Lily P. Almeida, Caroline S. M. Mattos, Ana Márcia Mendonça, Ana Carolina M. Alves, Márcio J. Pinheiro, Aimara C. Porrozzi, Renato Abass, Elfadil Ulrich, Steinhoff Teixeira, Henrique C. |
| dc.subject.proposal.spa.fl_str_mv |
Canine visceral leishmaniasis Serodiagnosis rKLO8 rK26 |
| topic |
Canine visceral leishmaniasis Serodiagnosis rKLO8 rK26 |
| description |
Artículo digital. |
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2019 |
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2019-11-06T14:31:19Z |
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2019-11-06T14:31:19Z |
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2019-11-05 |
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Artículo de revista |
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Martínez, L. P., Almeida, C. S., M., A. M., Mendonca, A. C., Alves, M. J., Pinheiro, A. C., Porozzi, R., Abass, E., Steinhoff, U. & Teixeira, H. C. (2016). Diagnostic accuracy of rklo8 versus rk26 elisas for screening of canine visceral leishmaniasis. Acta Tropica. 1 – 23. |
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0001-706X. |
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https://repositorio.unisucre.edu.co/handle/001/955 |
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http://dx.doi.org/doi:10.1016/j.actatropica.2016.11.021. |
| identifier_str_mv |
Martínez, L. P., Almeida, C. S., M., A. M., Mendonca, A. C., Alves, M. J., Pinheiro, A. C., Porozzi, R., Abass, E., Steinhoff, U. & Teixeira, H. C. (2016). Diagnostic accuracy of rklo8 versus rk26 elisas for screening of canine visceral leishmaniasis. Acta Tropica. 1 – 23. 0001-706X. |
| url |
https://repositorio.unisucre.edu.co/handle/001/955 http://dx.doi.org/doi:10.1016/j.actatropica.2016.11.021. |
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eng |
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eng |
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Articulo de revista |
| dc.relation.references.spa.fl_str_mv |
Abass, E., Bollig, N., Reinhard, K., Camara, B., Mansour, D., Visekruna, A., Lohoff, M., Steinhoff, U., 2013. rKLO8, a Novel Leishmania donovani - derived recombinant immunodominant protein for sensitive detection of visceral leishmaniasis in Sudan. PLoS Neglected Trop. Dis. 7. doi: 10.1371/journal.pntd.0002322. Abass, E., Kang, C., Martinkovic, F., Semião-Santos, S., Sundar, S., Walden, P., Piarroux, R., el Harith, A., Lohoff, M., Steinhoff, U., 2015. Heterogeneity of Leishmania donovani parasites complicates diagnosis of visceral leishmaniasis: comparison of different serological tests in three endemic regions. PLoS One 10, e0116408. doi: 10.1371/journal.pone.0116408. Alves, A.S., Mouta-Confort, E., Figueiredo, F.B., Oliveira, R.V.C., Schubach, A.O., Madeira, M.F., 2012. Evaluation of serological cross-reactivity between canine visceral leishmaniasis and natural infection by Trypanosoma caninum. Res Vet Sci, v. 93, n. 3, p. 1329-33. doi: 10.1016/j.rvsc.2012.07.006. Asl, A.S., Sharifi, M., Mohebali, M., Zarei, Z., Babaloo, Z., Akhoundi, B., 2013. Evaluation of canine anti-Leishmania IgG subclasses and their relation with skin signs in naturally infected dogs in the northwest of iran. Turk. J. Vet. Anim. Sci. 37, 512-515. doi:10.3906/vet-1112-24. Barata, R.A., Peixoto, J.C., Tanure, A., Gomes, M.E., Apolinário, E.C., Bodevan, E.C., De Araújo, H.S., Dias, E.S., Pinheiro, A.D.C., 2013. Epidemiology of visceral leishmaniasis in a reemerging focus of intense transmission in Minas Gerais State, Brazil. BioMed Res. Int. 2013. doi: 10.1155/2013/405083. |
| dc.rights.spa.fl_str_mv |
Derechos Reservados - Universidad de Sucre, 2019 |
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Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) |
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Inglaterra: Acta trópica, 2016. |
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https://www.researchgate.net/publication/310593359. |
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Martínez Abad, Lily P.09aa118f4ed78bdbea6a51af6d80213f-1Almeida, Caroline S.9e1ae2db7017376d300dfbc0112d6e8d-1M. Mattos, Ana Márciae9a09d4b82ebd328a12b6b5775fac277-1Mendonça, Ana Carolina3ce7c77b75adbd9dd6b06e73df1e621c-1M. Alves, Márcio J.5ca345c65c89b67a9e359bc145fbaf7a-1Pinheiro, Aimara C.0a8d8e394e200bd90b58e96a126df01c-1Porrozzi, Renato714f57bfefd531f8730389c5615fb571-1Abass, Elfadil2cb5f3a7c379b834e90f07c82ae26877-1Ulrich, Steinhoffa66d7201598a846d2a66e1b6f6448c55-1Teixeira, Henrique C.5559f22dcd54d9bb823fb2bfa05709ef-1Colombia2019-11-06T14:31:19Z2019-11-06T14:31:19Z2019-11-05Martínez, L. P., Almeida, C. S., M., A. M., Mendonca, A. C., Alves, M. J., Pinheiro, A. C., Porozzi, R., Abass, E., Steinhoff, U. & Teixeira, H. C. (2016). Diagnostic accuracy of rklo8 versus rk26 elisas for screening of canine visceral leishmaniasis. Acta Tropica. 1 – 23.0001-706X.https://repositorio.unisucre.edu.co/handle/001/955http://dx.doi.org/doi:10.1016/j.actatropica.2016.11.021.Artículo digital.Canine visceral leishmaniasis (CVL) represents an important public health issue. Despite numerous diagnostic tests available, CVL diagnosis still needs to be improved to achieve a more accurate detection rate. Recently, rKLO8, a new antigenic protein of Sudanese Leishmania donovani, was studied for the first time in diagnosis of human visceral leishmaniasis (HVL) and showed good performance. The present study aimed to evaluate serum reactivity to rKL08 and the reference antigen rK26, and to compare both diagnostic proteins with the combined DPP® CVL rapid test and ELISA (EIE-Bio-Manguinhos) confirmatory test, which are both recommended for the diagnosis of CVL in Brazil. Serum samples of dogs were grouped into: (I) DPP®/EIE negative (n=100) and (II) DPP®/EIE positive sera (n=100). Enhanced levels of IgG, mainly IgG2, to both rKLO8 and rK26 were found in group II. Sensitivity was 68% and 77% and specificity was 92% and 91%, for rKLO8 and rK26 antigens, respectively. Moreover, the combination of rKLO8 and rK26 antigens (rKLO8+rK26) exhibited higher sensitivity (85%) and specificity (93%). Thus, our results show that apart from the improved diagnostic power of rKLO8 in HVL, this new antigen is also suitable for the diagnosis of CVL. Further, the combination of rKLO8 and rK26 antigens increases the diagnostic accuracy of CVL.application/pdfengInglaterra: Acta trópica, 2016.Articulo de revistaAbass, E., Bollig, N., Reinhard, K., Camara, B., Mansour, D., Visekruna, A., Lohoff, M., Steinhoff, U., 2013. rKLO8, a Novel Leishmania donovani - derived recombinant immunodominant protein for sensitive detection of visceral leishmaniasis in Sudan. PLoS Neglected Trop. Dis. 7. doi: 10.1371/journal.pntd.0002322.Abass, E., Kang, C., Martinkovic, F., Semião-Santos, S., Sundar, S., Walden, P., Piarroux, R., el Harith, A., Lohoff, M., Steinhoff, U., 2015. Heterogeneity of Leishmania donovani parasites complicates diagnosis of visceral leishmaniasis: comparison of different serological tests in three endemic regions. PLoS One 10, e0116408. doi: 10.1371/journal.pone.0116408.Alves, A.S., Mouta-Confort, E., Figueiredo, F.B., Oliveira, R.V.C., Schubach, A.O., Madeira, M.F., 2012. Evaluation of serological cross-reactivity between canine visceral leishmaniasis and natural infection by Trypanosoma caninum. Res Vet Sci, v. 93, n. 3, p. 1329-33. doi: 10.1016/j.rvsc.2012.07.006.Asl, A.S., Sharifi, M., Mohebali, M., Zarei, Z., Babaloo, Z., Akhoundi, B., 2013. Evaluation of canine anti-Leishmania IgG subclasses and their relation with skin signs in naturally infected dogs in the northwest of iran. Turk. J. Vet. Anim. Sci. 37, 512-515. doi:10.3906/vet-1112-24.Barata, R.A., Peixoto, J.C., Tanure, A., Gomes, M.E., Apolinário, E.C., Bodevan, E.C., De Araújo, H.S., Dias, E.S., Pinheiro, A.D.C., 2013. Epidemiology of visceral leishmaniasis in a reemerging focus of intense transmission in Minas Gerais State, Brazil. BioMed Res. Int. 2013. doi: 10.1155/2013/405083.Derechos Reservados - Universidad de Sucre, 2019https://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)http://purl.org/coar/access_right/c_abf2https://www.researchgate.net/publication/310593359.Diagnostic accuracy of rKLO8 versus rK26 ELISAs for screening of canine visceral leishmaniasis.Artículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501Texthttp://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Canine visceral leishmaniasisSerodiagnosisrKLO8rK26PublicationORIGINALDiagnostic accuracy of rklo8 versus rk26 Elisas for screening of canine visceral leishmaniasis..pdfDiagnostic accuracy of rklo8 versus rk26 Elisas for screening of canine visceral leishmaniasis..pdfapplication/pdf396343https://repositorio.unisucre.edu.co/bitstreams/a2e3b656-0d73-423d-a78b-5db7fc46fcc5/download255ffe34b3981940e4047050389bc6a3MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81366https://repositorio.unisucre.edu.co/bitstreams/b65b3f51-0be9-4707-aef0-53e7ef9ad39a/download5f839364c91422e4b2a78812717048fbMD52TEXTDiagnostic accuracy of rklo8 versus rk26 Elisas for screening of canine visceral leishmaniasis..pdf.txtDiagnostic accuracy of rklo8 versus rk26 Elisas for screening of canine visceral leishmaniasis..pdf.txtExtracted texttext/plain37916https://repositorio.unisucre.edu.co/bitstreams/9061c52a-f788-4208-8248-fb1d4df4df8c/download184d375433085220aaee9563b475c45bMD55THUMBNAILDiagnostic accuracy of rklo8 versus rk26 Elisas for screening of canine visceral leishmaniasis..pdf.jpgDiagnostic accuracy of rklo8 versus rk26 Elisas for screening of canine visceral leishmaniasis..pdf.jpgGenerated Thumbnailimage/jpeg10357https://repositorio.unisucre.edu.co/bitstreams/8efcae06-e8ed-44e2-866a-df5db4070e62/downloadaaf4a5894b043e28de46ff10995f0a07MD56001/955oai:repositorio.unisucre.edu.co:001/9552024-04-17 16:30:38.848https://creativecommons.org/licenses/by-nc/4.0/Derechos Reservados - Universidad de Sucre, 2019open.accesshttps://repositorio.unisucre.edu.coRepositorio Institucional Universidad de Sucrebdigital@metabiblioteca.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 |