L-Asparaginase from E. chrysanthemi expressed in glycoswitch: effect of Hist-Tag fusion on the extracellular expression.
Artículo digital.
- Autores:
-
Effer, Brian
Meira Lima, Guilherme
Cabarca, Sindy
Pessoa, Adalberto
Farías, Jorge G.
Monteiro, Gisele
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2019
- Institución:
- Universidad de Sucre
- Repositorio:
- Repositorio Unisucre
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.unisucre.edu.co:001/951
- Acceso en línea:
- https://repositorio.unisucre.edu.co/handle/001/951
https://doi.org/10.1080/10826068.2019.1599396.
- Palabra clave:
- L-Asparaginase
Extracellular expression
Glycoswitch
His-tag
- Rights
- openAccess
- License
- Derechos Reservados - Universidad de Sucre, 2019
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| dc.title.spa.fl_str_mv |
L-Asparaginase from E. chrysanthemi expressed in glycoswitch: effect of Hist-Tag fusion on the extracellular expression. |
| title |
L-Asparaginase from E. chrysanthemi expressed in glycoswitch: effect of Hist-Tag fusion on the extracellular expression. |
| spellingShingle |
L-Asparaginase from E. chrysanthemi expressed in glycoswitch: effect of Hist-Tag fusion on the extracellular expression. L-Asparaginase Extracellular expression Glycoswitch His-tag |
| title_short |
L-Asparaginase from E. chrysanthemi expressed in glycoswitch: effect of Hist-Tag fusion on the extracellular expression. |
| title_full |
L-Asparaginase from E. chrysanthemi expressed in glycoswitch: effect of Hist-Tag fusion on the extracellular expression. |
| title_fullStr |
L-Asparaginase from E. chrysanthemi expressed in glycoswitch: effect of Hist-Tag fusion on the extracellular expression. |
| title_full_unstemmed |
L-Asparaginase from E. chrysanthemi expressed in glycoswitch: effect of Hist-Tag fusion on the extracellular expression. |
| title_sort |
L-Asparaginase from E. chrysanthemi expressed in glycoswitch: effect of Hist-Tag fusion on the extracellular expression. |
| dc.creator.fl_str_mv |
Effer, Brian Meira Lima, Guilherme Cabarca, Sindy Pessoa, Adalberto Farías, Jorge G. Monteiro, Gisele |
| dc.contributor.author.spa.fl_str_mv |
Effer, Brian Meira Lima, Guilherme Cabarca, Sindy Pessoa, Adalberto Farías, Jorge G. Monteiro, Gisele |
| dc.subject.proposal.spa.fl_str_mv |
L-Asparaginase Extracellular expression Glycoswitch His-tag |
| topic |
L-Asparaginase Extracellular expression Glycoswitch His-tag |
| description |
Artículo digital. |
| publishDate |
2019 |
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2019-11-01T05:22:03Z |
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2019-11-01T05:22:03Z |
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2019-10-31 |
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Artículo de revista |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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Effer, B., Meira, G., Cabarca, S., Pessoa, A., Farías, J. G. & Monteiro, G. (2019). L-Asparaginase from E. chrysanthemi expressed in glycoswitch: effect of Hist-Tag fusion on the extracellular expression. Preparative Biochemistry and Biotechnology. 1 – 7. |
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1082-6068. |
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https://repositorio.unisucre.edu.co/handle/001/951 |
| dc.identifier.doi.spa.fl_str_mv |
https://doi.org/10.1080/10826068.2019.1599396. |
| identifier_str_mv |
Effer, B., Meira, G., Cabarca, S., Pessoa, A., Farías, J. G. & Monteiro, G. (2019). L-Asparaginase from E. chrysanthemi expressed in glycoswitch: effect of Hist-Tag fusion on the extracellular expression. Preparative Biochemistry and Biotechnology. 1 – 7. 1082-6068. |
| url |
https://repositorio.unisucre.edu.co/handle/001/951 https://doi.org/10.1080/10826068.2019.1599396. |
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eng |
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eng |
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Articulo de revista |
| dc.relation.references.spa.fl_str_mv |
Kotzia, G. A.; Labrou, N. E. L-Asparaginase from Erwinia chrysanthemi 3937: Cloning, Expression and Characterization. J. Biotechnol. 2007, 127, 657–669. DOI: 10.1016/ j.jbiotec.2006.07.037. Chien, W. W.; Allas, S.; Rachinel, N.; Sahakian, P.; Julien, M.; Le Beux, C.; Lacroix, C. E.; Abribat, T.; Salles, G. Pharmacology, Immunogenicity, and Efficacy of a Novel Pegylated Recombinant Erwinia chrysanthemi-Derived LAsparaginase. Invest. New Drugs 2014, 32, 795–805. DOI: 10.1007/s10637-014-0102-9. Chen, R. Bacterial Expression Systems for Recombinant Protein Production: E. coli and Beyond. Biotechnol. Adv. 2012, 30, 1102–1107. DOI: 10.1016/j.biotechadv.2011.09.013. Terpe, K. Overview of Bacterial Expression Systems for Heterologous Protein Production: From Molecular and Biochemical Fundamentals to Commercial Systems. Appl. Microbiol. Biotechnol. 2006, 72, 211–222. DOI: 10.1007/s00253- 006-0465-8. Khow, O.; Suntrarachun, S. Strategies for Production of Active Eukaryotic Proteins in Bacterial Expression System. Asian Pac. J. Trop. Biomed. 2012, 2, 159–162. DOI: 10.1016/S2221- 1691(11)60213-X. |
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Derechos Reservados - Universidad de Sucre, 2019 |
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info:eu-repo/semantics/openAccess |
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Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) |
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Derechos Reservados - Universidad de Sucre, 2019 https://creativecommons.org/licenses/by-nc/4.0/ Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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application/pdf |
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Colombia |
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Reino Unido: Preparative Biochemistry and Biotechnology, 2019. |
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https://www.ncbi.nlm.nih.gov/pubmed/30990115. |
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Universidad de Sucre |
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Effer, Brianb52ce0aca050eccd1b12bb76bb97be9d-1Meira Lima, Guilherme473d388bba0c9fb0dbefe7b2cd9172ba-1Cabarca, Sindyb1d7850b91ea7fbda06b45ba41dd2b2c-1Pessoa, Adalbertoa1174a6a611baa9c1b96eeec87f69501-1Farías, Jorge G.d15d69bcc7a348c5de18c0a36725c6e9-1Monteiro, Gisele9d205c9ec1307e6a5287f00e9bc4afdf-1Colombia2019-11-01T05:22:03Z2019-11-01T05:22:03Z2019-10-31Effer, B., Meira, G., Cabarca, S., Pessoa, A., Farías, J. G. & Monteiro, G. (2019). L-Asparaginase from E. chrysanthemi expressed in glycoswitch: effect of Hist-Tag fusion on the extracellular expression. Preparative Biochemistry and Biotechnology. 1 – 7.1082-6068.https://repositorio.unisucre.edu.co/handle/001/951https://doi.org/10.1080/10826068.2019.1599396.Artículo digital.L-Asparaginase (L-ASNase) is an important enzyme used to treat acute lymphoblastic leukemia, recombinantly produced in a prokaryotic expression system. Exploration of alternatives production systems like as extracellular expression in microorganisms generally recognized as safe (such as Pichia pastoris GlycoswitchVR ) could be advantageous, in particular, if this system is able to produce homogeneous glycosylation. Here, we evaluated extracellular expression into GlycoswitchVR using two different strains constructions containing the asnB gene coding for Erwinia chrysanthemi L-ASNase (with and without His-tag), in order to find the best system for producing the extracellular and biologically active protein. When the His-tag was absent, both cell expression and protein secretion processes were considerably improved. Three-dimensional modeling of the protein suggests that additional structures (His-tag) could adversely affect native conformation and folding from L-ASNase and therefore the expression and cell secretion of this enzyme.application/pdfengReino Unido: Preparative Biochemistry and Biotechnology, 2019.Articulo de revistaKotzia, G. A.; Labrou, N. E. L-Asparaginase from Erwinia chrysanthemi 3937: Cloning, Expression and Characterization. J. Biotechnol. 2007, 127, 657–669. DOI: 10.1016/ j.jbiotec.2006.07.037.Chien, W. W.; Allas, S.; Rachinel, N.; Sahakian, P.; Julien, M.; Le Beux, C.; Lacroix, C. E.; Abribat, T.; Salles, G. Pharmacology, Immunogenicity, and Efficacy of a Novel Pegylated Recombinant Erwinia chrysanthemi-Derived LAsparaginase. Invest. New Drugs 2014, 32, 795–805. DOI: 10.1007/s10637-014-0102-9.Chen, R. Bacterial Expression Systems for Recombinant Protein Production: E. coli and Beyond. Biotechnol. Adv. 2012, 30, 1102–1107. DOI: 10.1016/j.biotechadv.2011.09.013.Terpe, K. Overview of Bacterial Expression Systems for Heterologous Protein Production: From Molecular and Biochemical Fundamentals to Commercial Systems. Appl. Microbiol. Biotechnol. 2006, 72, 211–222. DOI: 10.1007/s00253- 006-0465-8.Khow, O.; Suntrarachun, S. Strategies for Production of Active Eukaryotic Proteins in Bacterial Expression System. Asian Pac. J. Trop. Biomed. 2012, 2, 159–162. DOI: 10.1016/S2221- 1691(11)60213-X.Derechos Reservados - Universidad de Sucre, 2019https://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)http://purl.org/coar/access_right/c_abf2https://www.ncbi.nlm.nih.gov/pubmed/30990115.L-Asparaginase from E. chrysanthemi expressed in glycoswitch: effect of Hist-Tag fusion on the extracellular expression.Artículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501Texthttp://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85L-AsparaginaseExtracellular expressionGlycoswitchHis-tagPublicationORIGINALL-Asparaginase from E. chrysanthemi expressed in glycoswitch effect of His-Tag fusion on the extracellular expression..pdfL-Asparaginase from E. chrysanthemi expressed in glycoswitch effect of His-Tag fusion on the extracellular expression..pdfapplication/pdf1484259https://repositorio.unisucre.edu.co/bitstreams/96e261a0-cbb7-4306-a5cd-32f1c9e6c66e/downloada79a0ce1408feaad22cac9fa9195f29eMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81366https://repositorio.unisucre.edu.co/bitstreams/85a015fd-cc09-4fbd-9874-d40d63ef490b/download5f839364c91422e4b2a78812717048fbMD52TEXTL-Asparaginase from E. chrysanthemi expressed in glycoswitch effect of His-Tag fusion on the extracellular expression..pdf.txtL-Asparaginase from E. chrysanthemi expressed in glycoswitch effect of His-Tag fusion on the extracellular expression..pdf.txtExtracted texttext/plain30857https://repositorio.unisucre.edu.co/bitstreams/dca9d7a8-42fe-4fc6-80a7-af043d838cc8/downloade7e05bade09addcb03578f45d85c4fb9MD55THUMBNAILL-Asparaginase from E. chrysanthemi expressed in glycoswitch effect of His-Tag fusion on the extracellular expression..pdf.jpgL-Asparaginase from E. chrysanthemi expressed in glycoswitch effect of His-Tag fusion on the extracellular expression..pdf.jpgGenerated Thumbnailimage/jpeg9765https://repositorio.unisucre.edu.co/bitstreams/d49e3136-9c37-496e-a0b2-fa2563d1de35/downloadc9608b2d9fa859e720b30f2c48aae5d2MD56001/951oai:repositorio.unisucre.edu.co:001/9512024-04-17 16:30:59.84https://creativecommons.org/licenses/by-nc/4.0/Derechos Reservados - Universidad de Sucre, 2019open.accesshttps://repositorio.unisucre.edu.coRepositorio Institucional Universidad de Sucrebdigital@metabiblioteca.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 |