In Vivo Indicators of Cytoplasmic, Vacuolar, and Extracellular pH Using pHluorin2 in Candida albicans.
Artículo digital.
- Autores:
-
Tournu, Heléne
Luna Tapia, Arturo
Peters, Brian M.
Palmer, Glen E.
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2019
- Institución:
- Universidad de Sucre
- Repositorio:
- Repositorio Unisucre
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.unisucre.edu.co:001/791
- Acceso en línea:
- https://repositorio.unisucre.edu.co/handle/001/791
- Palabra clave:
- Candida albicans
Chemical screening
PH dynamics
Vacuoles
- Rights
- openAccess
- License
- Derechos Reservados - Universidad de Sucre, 2019
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| dc.title.spa.fl_str_mv |
In Vivo Indicators of Cytoplasmic, Vacuolar, and Extracellular pH Using pHluorin2 in Candida albicans. |
| title |
In Vivo Indicators of Cytoplasmic, Vacuolar, and Extracellular pH Using pHluorin2 in Candida albicans. |
| spellingShingle |
In Vivo Indicators of Cytoplasmic, Vacuolar, and Extracellular pH Using pHluorin2 in Candida albicans. Candida albicans Chemical screening PH dynamics Vacuoles |
| title_short |
In Vivo Indicators of Cytoplasmic, Vacuolar, and Extracellular pH Using pHluorin2 in Candida albicans. |
| title_full |
In Vivo Indicators of Cytoplasmic, Vacuolar, and Extracellular pH Using pHluorin2 in Candida albicans. |
| title_fullStr |
In Vivo Indicators of Cytoplasmic, Vacuolar, and Extracellular pH Using pHluorin2 in Candida albicans. |
| title_full_unstemmed |
In Vivo Indicators of Cytoplasmic, Vacuolar, and Extracellular pH Using pHluorin2 in Candida albicans. |
| title_sort |
In Vivo Indicators of Cytoplasmic, Vacuolar, and Extracellular pH Using pHluorin2 in Candida albicans. |
| dc.creator.fl_str_mv |
Tournu, Heléne Luna Tapia, Arturo Peters, Brian M. Palmer, Glen E. |
| dc.contributor.author.spa.fl_str_mv |
Tournu, Heléne Luna Tapia, Arturo Peters, Brian M. Palmer, Glen E. |
| dc.contributor.corporatename.spa.fl_str_mv |
American Society for Microbiology. |
| dc.subject.proposal.spa.fl_str_mv |
Candida albicans Chemical screening PH dynamics Vacuoles |
| topic |
Candida albicans Chemical screening PH dynamics Vacuoles |
| description |
Artículo digital. |
| publishDate |
2019 |
| dc.date.accessioned.spa.fl_str_mv |
2019-09-24T21:38:38Z |
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2019-09-24T21:38:38Z |
| dc.date.issued.spa.fl_str_mv |
2019-09-24 |
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Artículo de revista |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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Tournu, H., Luna, A., Peters, B.M. & Palmer, G.E. (2017). In Vivo Indicators of Cytoplasmic, Vacuolar, and Extracellular pH Using pHluorin2 in Candida albicans. Revista mSphere . 2(4). |
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1098-6596 |
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https://repositorio.unisucre.edu.co/handle/001/791 |
| dc.identifier.doi.spa.fl_str_mv |
10.1128/mSphere.00276-17 |
| identifier_str_mv |
Tournu, H., Luna, A., Peters, B.M. & Palmer, G.E. (2017). In Vivo Indicators of Cytoplasmic, Vacuolar, and Extracellular pH Using pHluorin2 in Candida albicans. Revista mSphere . 2(4). 1098-6596 10.1128/mSphere.00276-17 |
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https://repositorio.unisucre.edu.co/handle/001/791 |
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eng |
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eng |
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Articulo de revista |
| dc.relation.references.spa.fl_str_mv |
Miesenböck G, De Angelis DA, Rothman JE. 1998. Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins. Nature 394:192–195. https://doi.org/10.1038/28190. Bencina M. 2013. Illumination of the spatial order of intracellular pH by genetically encoded pH-sensitive sensors. Sensors 13:16736 –16758. https://doi.org/10.3390/s131216736. Liu NN, Köhler JR. 2016. Antagonism of fluconazole and a proton pump inhibitor against Candida albicans. Antimicrob Agents Chemother 60: 1145–1147. https://doi.org/10.1128/AAC.02043-15. Prosser DC, Whitworth K, Wendland B. 2010. Quantitative analysis of endocytosis with cytoplasmic pHluorin chimeras. Traffic 11:1141–1150. https://doi.org/10.1111/j.1600-0854.2010.01088.x. Ho CY, Choy CH, Wattson CA, Johnson DE, Botelho RJ. 2015. The Fab1/PIKfyve phosphoinositide phosphate kinase is not necessary to maintain the pH of lysosomes and of the yeast vacuole. J Biol Chem 290:9919 –9928. https://doi.org/10.1074/jbc.M114.613984. |
| dc.rights.spa.fl_str_mv |
Derechos Reservados - Universidad de Sucre, 2019 |
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Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) |
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Derechos Reservados - Universidad de Sucre, 2019 https://creativecommons.org/licenses/by-nc/4.0/ Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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application/pdf |
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Colombia |
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Estados Unidos: mSphere, 2017. |
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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5497024/ |
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Universidad de Sucre |
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Tournu, Heléne3d93c0788043d1422bd25dfa3bf1bd87-1Luna Tapia, Arturo37137097b30746a987e4adcda146f579-1Peters, Brian M.6950b411f4c62c3a9b335f472f53acab-1Palmer, Glen E.5f5dd8dc79907c8395ffb4851dee7394-1American Society for Microbiology.Colombia2019-09-24T21:38:38Z2019-09-24T21:38:38Z2019-09-24Tournu, H., Luna, A., Peters, B.M. & Palmer, G.E. (2017). In Vivo Indicators of Cytoplasmic, Vacuolar, and Extracellular pH Using pHluorin2 in Candida albicans. Revista mSphere . 2(4).1098-6596https://repositorio.unisucre.edu.co/handle/001/79110.1128/mSphere.00276-17Artículo digital.Environmental or chemically induced stresses often trigger physiological responses that regulate intracellular pH. As such, the capacity to detect pH changes in real time and within live cells is of fundamental importance to essentially all aspects of biology. In this respect, pHluorin, a pH-sensitive variant of green fluorescent protein, has provided an invaluable tool to detect such responses. Here, we report the adaptation of pHluorin2 (PHL2), a substantially brighter variant of pHluorin, for use with the human fungal pathogen Candida albicans. As well as a cytoplasmic PHL2 indicator, we describe a version that specifically localizes within the fungal vacuole, an acidified subcellular compartment with important functions in nutrient storage and pH homeostasis. In addition, by means of a glycophosphatidylinositolanchored PHL2-fusion protein, we generated a cell surface pH sensor.application/pdfengEstados Unidos: mSphere, 2017.Articulo de revistaMiesenböck G, De Angelis DA, Rothman JE. 1998. Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins. Nature 394:192–195. https://doi.org/10.1038/28190.Bencina M. 2013. Illumination of the spatial order of intracellular pH by genetically encoded pH-sensitive sensors. Sensors 13:16736 –16758. https://doi.org/10.3390/s131216736.Liu NN, Köhler JR. 2016. Antagonism of fluconazole and a proton pump inhibitor against Candida albicans. Antimicrob Agents Chemother 60: 1145–1147. https://doi.org/10.1128/AAC.02043-15.Prosser DC, Whitworth K, Wendland B. 2010. Quantitative analysis of endocytosis with cytoplasmic pHluorin chimeras. Traffic 11:1141–1150. https://doi.org/10.1111/j.1600-0854.2010.01088.x.Ho CY, Choy CH, Wattson CA, Johnson DE, Botelho RJ. 2015. The Fab1/PIKfyve phosphoinositide phosphate kinase is not necessary to maintain the pH of lysosomes and of the yeast vacuole. J Biol Chem 290:9919 –9928. https://doi.org/10.1074/jbc.M114.613984.Derechos Reservados - Universidad de Sucre, 2019https://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)http://purl.org/coar/access_right/c_abf2https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5497024/In Vivo Indicators of Cytoplasmic, Vacuolar, and Extracellular pH Using pHluorin2 in Candida albicans.Artículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501Texthttp://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Candida albicansChemical screeningPH dynamicsVacuolesPublicationORIGINALIn Vivo Indicators of Cytoplasmic, Vacuolar, and Extracellular pH Using pHluorin2 in Candida albicans.pdfIn Vivo Indicators of Cytoplasmic, Vacuolar, and Extracellular pH Using pHluorin2 in Candida albicans.pdfapplication/pdf1474277https://repositorio.unisucre.edu.co/bitstreams/bb82020d-00b1-4815-92a0-be94fb3237d9/download5f878730ca15eda231f92d31dbdb8f99MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81366https://repositorio.unisucre.edu.co/bitstreams/40957407-1f51-4ddd-b8c1-a45e4a6f9555/download5f839364c91422e4b2a78812717048fbMD52TEXTIn Vivo Indicators of Cytoplasmic, Vacuolar, and Extracellular pH Using pHluorin2 in Candida albicans.pdf.txtIn Vivo Indicators of Cytoplasmic, Vacuolar, and Extracellular pH Using pHluorin2 in Candida albicans.pdf.txtExtracted texttext/plain53138https://repositorio.unisucre.edu.co/bitstreams/5b025ebc-bcc9-4ed4-95b7-a1e87eee5182/downloadb8e67ccd6fb515a8197460e312768736MD55THUMBNAILIn Vivo Indicators of Cytoplasmic, Vacuolar, and Extracellular pH Using pHluorin2 in Candida albicans.pdf.jpgIn Vivo Indicators of Cytoplasmic, Vacuolar, and Extracellular pH Using pHluorin2 in Candida albicans.pdf.jpgGenerated Thumbnailimage/jpeg14410https://repositorio.unisucre.edu.co/bitstreams/fe44cec0-5969-4215-8591-deddb2375840/downloada2056c9843bd4476f87ddb809e25e929MD56001/791oai:repositorio.unisucre.edu.co:001/7912024-04-17 16:31:16.689https://creativecommons.org/licenses/by-nc/4.0/Derechos Reservados - Universidad de Sucre, 2019open.accesshttps://repositorio.unisucre.edu.coRepositorio Institucional Universidad de Sucrebdigital@metabiblioteca.comYXV0b3Jpem8gYSBsYSBVTklWRVJTSURBRCBERSBTVUNSRSwgcGFyYToKCkkuCVF1ZSBlbiBsb3MgdMOpcm1pbm9zIGVzdGFibGVjaWRvcyBlbiBsYSBMZXkgMjMgZGUgMTk4MiwgTGV5IDQ0IGRlIDE5OTMsIERlY2lzacOzbiBBbmRpbmEgMzUxIGRlIDE5OTMsIERlY3JldG8gNDYwIGRlIDE5OTUgeSBkZW3DoXMgbm9ybWFzIGdlbmVyYWxlcyBzb2JyZSBsYSBtYXRlcmlhLCB1dGlsaWNlIHkgdXNlIGVuIHRvZGFzIHN1cyBmb3JtYXMsIGxvcyBkZXJlY2hvcyBwYXRyaW1vbmlhbGVzIGRlIGVkaWNpw7NuLCByZXByb2R1Y2Npw7NuLCBjb211bmljYWNpw7NuIHDDumJsaWNhIHkgZGlzdHJpYnVjacOzbiAoYWxxdWlsZXIsIHByw6lzdGFtbyBww7pibGljbyBlIGltcG9ydGFjacOzbikgcXVlIG1lIGNvcnJlc3BvbmRlbiBjb21vIGNyZWFkb3IgZGUgbGEgb2JyYSBvYmpldG8gZGVsIHByZXNlbnRlIGRvY3VtZW50by4gIAoKSUkuCUxhIHByZXNlbnRlIGF1dG9yaXphY2nDs24gc2UgaGFjZSBleHRlbnNpdmEgbm8gc8OzbG8gYSBsYXMgZmFjdWx0YWRlcyB5IGRlcmVjaG9zIGRlIHVzbyBzb2JyZSBsYSBvYnJhIGVuIGZvcm1hdG8gbyBzb3BvcnRlIG1hdGVyaWFsLCBzaW5vIHRhbWJpw6luIHBhcmEgZm9ybWF0byB2aXJ0dWFsLCBlbGVjdHLDs25pY28sIGRpZ2l0YWwsIMOzcHRpY28sIHVzb3MgZW4gcmVkLCBJbnRlcm5ldCwgaW50cmFuZXQsIGV0Yy4sIHkgZW4gZ2VuZXJhbCBwYXJhIGN1YWxxdWllciBmb3JtYXRvIGNvbm9jaWRvIG8gcG9yIGNvbm9jZXIuCgpJSUkuCUVMIEFVVE9SIOKAkyBBVVRPUkVTLCBtYW5pZmllc3RhbiBxdWUgbGEgb2JyYSBvYmpldG8gZGUgbGEgcHJlc2VudGUgYXV0b3JpemFjacOzbiBlcyBvcmlnaW5hbCB5IGxhIHJlYWxpesOzIHNpbiB2aW9sYXIgbyB1c3VycGFyIGRlcmVjaG9zIGRlIGF1dG9yIGRlIHRlcmNlcm9zLCBwb3IgbG8gdGFudG8gbGEgb2JyYSBlcyBkZSBzdSBleGNsdXNpdmEgYXV0b3LDrWEgeSBkZXRlbnRhIGxhIHRpdHVsYXJpZGFkIHNvYnJlIGxhIG1pc21hLiAgCgpJVi4JRW4gY2FzbyBkZSBwcmVzZW50YXJzZSBjdWFscXVpZXIgcmVjbGFtYWNpw7NuIG8gYWNjacOzbiBwb3IgcGFydGUgZGUgdW4gdGVyY2VybyBlbiBjdWFudG8gYSBsb3MgZGVyZWNob3MgZGUgYXV0b3Igc29icmUgbGEgb2JyYSBlbiBjdWVzdGnDs24sIEVMIEVTVFVESUFOVEUg4oCTIEFVVE9SLCBhc3VtaXLDoSB0b2RhICBsYSByZXNwb25zYWJpbGlkYWQsIHkgc2FsZHLDoSBlbiBkZWZlbnNhICBkZSBsb3MgZGVyZWNob3MgYXF1w60gYXV0b3JpemFkb3M7ICBwYXJhIHRvZG9zIGxvcyBlZmVjdG9zIGxhIFVuaXZlcnNpZGFkIGFjdMO6YSBjb21vIHVuIHRlcmNlcm8gZGUgYnVlbmEgZmUuCg== |