Endosomal Trafficking Defects Can Induce Calcium-Dependent Azole Tolerance in Candida albicans.
Artículo digital
- Autores:
-
Luna Tapia, Arturo
Tournu, Héléne
Peters, Tracy L.
Palmer, Glen E.
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2019
- Institución:
- Universidad de Sucre
- Repositorio:
- Repositorio Unisucre
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.unisucre.edu.co:001/800
- Acceso en línea:
- https://repositorio.unisucre.edu.co/handle/001/800
- Palabra clave:
- Candida albicans
Azoles
Endosomal
- Rights
- openAccess
- License
- Derechos Reservados - Universidad de Sucre, 2019
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| dc.title.spa.fl_str_mv |
Endosomal Trafficking Defects Can Induce Calcium-Dependent Azole Tolerance in Candida albicans. |
| title |
Endosomal Trafficking Defects Can Induce Calcium-Dependent Azole Tolerance in Candida albicans. |
| spellingShingle |
Endosomal Trafficking Defects Can Induce Calcium-Dependent Azole Tolerance in Candida albicans. Candida albicans Azoles Endosomal |
| title_short |
Endosomal Trafficking Defects Can Induce Calcium-Dependent Azole Tolerance in Candida albicans. |
| title_full |
Endosomal Trafficking Defects Can Induce Calcium-Dependent Azole Tolerance in Candida albicans. |
| title_fullStr |
Endosomal Trafficking Defects Can Induce Calcium-Dependent Azole Tolerance in Candida albicans. |
| title_full_unstemmed |
Endosomal Trafficking Defects Can Induce Calcium-Dependent Azole Tolerance in Candida albicans. |
| title_sort |
Endosomal Trafficking Defects Can Induce Calcium-Dependent Azole Tolerance in Candida albicans. |
| dc.creator.fl_str_mv |
Luna Tapia, Arturo Tournu, Héléne Peters, Tracy L. Palmer, Glen E. |
| dc.contributor.author.spa.fl_str_mv |
Luna Tapia, Arturo Tournu, Héléne Peters, Tracy L. Palmer, Glen E. |
| dc.contributor.corporatename.spa.fl_str_mv |
American society for microbiology. |
| dc.subject.proposal.spa.fl_str_mv |
Candida albicans Azoles Endosomal |
| topic |
Candida albicans Azoles Endosomal |
| description |
Artículo digital |
| publishDate |
2019 |
| dc.date.accessioned.spa.fl_str_mv |
2019-09-26T22:15:59Z |
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2019-09-26T22:15:59Z |
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2019-09-26 |
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Artículo de revista |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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Luna, A., Tournu, H., Peters, T.L. & Palmer, G.E. ( 2016 ). Endosomal Trafficking Defects Can Induce Calcium-Dependent Azole Tolerance in Candida albicans. Antimicrobial Agents and Chemotherapy . 60(12), 7170 – 7177. |
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0066-4804 |
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https://repositorio.unisucre.edu.co/handle/001/800 |
| dc.identifier.doi.spa.fl_str_mv |
10.1128/AAC.01034-16 |
| identifier_str_mv |
Luna, A., Tournu, H., Peters, T.L. & Palmer, G.E. ( 2016 ). Endosomal Trafficking Defects Can Induce Calcium-Dependent Azole Tolerance in Candida albicans. Antimicrobial Agents and Chemotherapy . 60(12), 7170 – 7177. 0066-4804 10.1128/AAC.01034-16 |
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https://repositorio.unisucre.edu.co/handle/001/800 |
| dc.language.iso.spa.fl_str_mv |
eng |
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eng |
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Revista |
| dc.relation.references.spa.fl_str_mv |
Zhang YQ, Gamarra S, Garcia-Effron G, Park S, Perlin DS, Rao R. 2010. Requirement for ergosterol in V-ATPase function underlies antifungal activity of azole drugs. PLoS Pathog 6:e1000939. http://dx.doi.org/10 .1371/journal.ppat.1000939. Kelly SL, Lamb DC, Kelly DE, Manning NJ, Loeffler J, Hebart H, Schumacher U, Einsele H. 1997. Resistance to fluconazole and crossresistance to amphotericin B in Candida albicans from AIDS patients caused by defective sterol 5,6-desaturation. FEBS Lett 400:80–82. http: //dx.doi.org/10.1016/S0014-5793(96)01360-9. Vandeputte P, Ferrari S, Coste AT. 2012. Antifungal resistance and new strategies to control fungal infections. Int J Microbiol 2012:713687. http: //dx.doi.org/10.1155/2012/713687. Marichal P, Koymans L, Willemsens S, Bellens D, Verhasselt P, Luyten WH, Borgers M, Ramaekers F, Odds FC, Bossche HV. 1999. Contribution of mutations in the cytochrome P450 14alpha-demethylase (Erg11p, Cyp51p) to azole resistance in Candida albicans. Microbiology 145:2701– 2713. http://dx.doi.org/10.1099/00221287-145-10-2701. Perea S, Lopez-Ribot JL, Kirkpatrick WR, McAtee RK, Santillan RA, Martinez M, Calabrese D, Sanglard D, Patterson TF. 2001. Prevalence of molecular mechanisms of resistance to azole antifungal agents in Candida albicans strains displaying high-level fluconazole resistance isolated from human immunodeficiency virus-infected patients. Antimicrob Agents Chemother 45:2676–2684. http://dx.doi.org/10.1128/AAC.45.10.2676 -2684.2001. |
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Derechos Reservados - Universidad de Sucre, 2019 |
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info:eu-repo/semantics/openAccess |
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Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) |
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Derechos Reservados - Universidad de Sucre, 2019 https://creativecommons.org/licenses/by-nc/4.0/ Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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application/pdf |
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Colombia |
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Estados Unidos: Antimicrobial Agents and Chemotherapy , 2016. |
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https://www.ncbi.nlm.nih.gov › pubmed |
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Universidad de Sucre |
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Luna Tapia, Arturo37137097b30746a987e4adcda146f579-1Tournu, Héléne1e6baf5d7fd2c313312b39ad139ae9d3-1Peters, Tracy L.bad9d9e6b655cb0efdaecbf6a0383cc4-1Palmer, Glen E.5f5dd8dc79907c8395ffb4851dee7394-1American society for microbiology.Colombia2019-09-26T22:15:59Z2019-09-26T22:15:59Z2019-09-26Luna, A., Tournu, H., Peters, T.L. & Palmer, G.E. ( 2016 ). Endosomal Trafficking Defects Can Induce Calcium-Dependent Azole Tolerance in Candida albicans. Antimicrobial Agents and Chemotherapy . 60(12), 7170 – 7177.0066-4804https://repositorio.unisucre.edu.co/handle/001/80010.1128/AAC.01034-16Artículo digitalThe azole antifungals arrest fungal growth through inhibition of ergosterol biosynthesis. We recently reported that a Candida albicans vps21 / mutant, deficient in membrane trafficking through the late endosome/prevacuolar compartment (PVC), continues to grow in the presence of the azoles despite the depletion of cellular ergosterol. Here, we report that the vps21 / mutant exhibits less plasma membrane damage upon azole treatment than the wild type, as measured by the release of a cytoplasmic luciferase reporter into the culture supernatant. Our results also reveal that the vps21 / mutant has abnormal levels of intracellular Ca2 and, in the presence of fluconazole, enhanced expression of a calcineurin-responsive RTA2-GFP reporter. Furthermore, the azole tolerance phenotype of the vps21 / mutant is dependent upon both extracellular calcium levels and calcineurin activity. These findings underscore the importance of endosomal trafficking in determining the cellular consequences of azole treatment and indicate that this may occur through modulation of calcium- and calcineurin-dependent responses.application/pdfengEstados Unidos: Antimicrobial Agents and Chemotherapy , 2016.RevistaZhang YQ, Gamarra S, Garcia-Effron G, Park S, Perlin DS, Rao R. 2010. Requirement for ergosterol in V-ATPase function underlies antifungal activity of azole drugs. PLoS Pathog 6:e1000939. http://dx.doi.org/10 .1371/journal.ppat.1000939.Kelly SL, Lamb DC, Kelly DE, Manning NJ, Loeffler J, Hebart H, Schumacher U, Einsele H. 1997. Resistance to fluconazole and crossresistance to amphotericin B in Candida albicans from AIDS patients caused by defective sterol 5,6-desaturation. FEBS Lett 400:80–82. http: //dx.doi.org/10.1016/S0014-5793(96)01360-9.Vandeputte P, Ferrari S, Coste AT. 2012. Antifungal resistance and new strategies to control fungal infections. Int J Microbiol 2012:713687. http: //dx.doi.org/10.1155/2012/713687.Marichal P, Koymans L, Willemsens S, Bellens D, Verhasselt P, Luyten WH, Borgers M, Ramaekers F, Odds FC, Bossche HV. 1999. Contribution of mutations in the cytochrome P450 14alpha-demethylase (Erg11p, Cyp51p) to azole resistance in Candida albicans. Microbiology 145:2701– 2713. http://dx.doi.org/10.1099/00221287-145-10-2701.Perea S, Lopez-Ribot JL, Kirkpatrick WR, McAtee RK, Santillan RA, Martinez M, Calabrese D, Sanglard D, Patterson TF. 2001. Prevalence of molecular mechanisms of resistance to azole antifungal agents in Candida albicans strains displaying high-level fluconazole resistance isolated from human immunodeficiency virus-infected patients. Antimicrob Agents Chemother 45:2676–2684. http://dx.doi.org/10.1128/AAC.45.10.2676 -2684.2001.Derechos Reservados - Universidad de Sucre, 2019https://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)http://purl.org/coar/access_right/c_abf2https://www.ncbi.nlm.nih.gov › pubmedEndosomal Trafficking Defects Can Induce Calcium-Dependent Azole Tolerance in Candida albicans.Artículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501Texthttp://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Candida albicansAzolesEndosomalPublicationORIGINALAntimicrobial Agents and Chemotherapy-2016-Luna-Tapia-7170.full.pdfAntimicrobial Agents and Chemotherapy-2016-Luna-Tapia-7170.full.pdfapplication/pdf1327507https://repositorio.unisucre.edu.co/bitstreams/395acec7-d894-4a81-a5ce-3c35cb456b90/download5d32e76644e0208cce4b74798659b050MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81366https://repositorio.unisucre.edu.co/bitstreams/3586be68-0fde-4379-bf35-55bfed80c5fa/download5f839364c91422e4b2a78812717048fbMD52TEXTAntimicrobial Agents and Chemotherapy-2016-Luna-Tapia-7170.full.pdf.txtAntimicrobial Agents and Chemotherapy-2016-Luna-Tapia-7170.full.pdf.txtExtracted texttext/plain44278https://repositorio.unisucre.edu.co/bitstreams/7838eb6b-a5f1-48ff-9e10-02dc09e52b00/downloadbf0eb82c5fb08d89f15bfa16c01ed511MD55THUMBNAILAntimicrobial Agents and Chemotherapy-2016-Luna-Tapia-7170.full.pdf.jpgAntimicrobial Agents and Chemotherapy-2016-Luna-Tapia-7170.full.pdf.jpgGenerated Thumbnailimage/jpeg17129https://repositorio.unisucre.edu.co/bitstreams/fabf7608-e54c-434a-b8fe-6d8575cd2b81/download3b50aa85e30650cb8d849c0207de9769MD56001/800oai:repositorio.unisucre.edu.co:001/8002024-12-05 09:51:50.09https://creativecommons.org/licenses/by-nc/4.0/Derechos Reservados - Universidad de Sucre, 2019open.accesshttps://repositorio.unisucre.edu.coRepositorio Institucional Universidad de Sucrebdigital@metabiblioteca.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 |