Análisis in silico de genes vinculados a la biosíntesis de ácido indolacético (AIA) asociados a aislamientos bacterianos de suelos agrícolas del Eje Cafetero

El ácido indolacético (AIA) es una de las principales fitohormonas responsables de la morfogénesis de las plantas y un metabolito secundario bacteriano de interés en las Bacterias Promotoras de Crecimiento Vegetal (BPCV). En las aplicaciones biotecnológicas que se dan en la agricultura, como la form...

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Autores:: Jaramillo Zárate, María José

Tipo de recurso:

Fecha de publicación:: 2024

Institución:: Universidad Libre

Repositorio:: RIU - Repositorio Institucional UniLibre

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oai_identifier_str	oai:repository.unilibre.edu.co:10901/30432
network_acronym_str	RULIBRE2
network_name_str	RIU - Repositorio Institucional UniLibre
repository_id_str
dc.title.spa.fl_str_mv	Análisis in silico de genes vinculados a la biosíntesis de ácido indolacético (AIA) asociados a aislamientos bacterianos de suelos agrícolas del Eje Cafetero
dc.title.alternative.spa.fl_str_mv	In silico analysis of genes linked to indoleacetic acid (IAA) biosynthesis associated with bacterial isolates from agricultural soils of the Eje Cafetero.
title	Análisis in silico de genes vinculados a la biosíntesis de ácido indolacético (AIA) asociados a aislamientos bacterianos de suelos agrícolas del Eje Cafetero
spellingShingle	Análisis in silico de genes vinculados a la biosíntesis de ácido indolacético (AIA) asociados a aislamientos bacterianos de suelos agrícolas del Eje Cafetero Ácidos indolacéticos Genes Enzimas Metabolismo BPCV (bacterias promotoras de crecimiento vegetal) Indoleacetic Acids Genes Enzymes Metabolism PGPB (Plant Growth Promoting Bacteria)
title_short	Análisis in silico de genes vinculados a la biosíntesis de ácido indolacético (AIA) asociados a aislamientos bacterianos de suelos agrícolas del Eje Cafetero
title_full	Análisis in silico de genes vinculados a la biosíntesis de ácido indolacético (AIA) asociados a aislamientos bacterianos de suelos agrícolas del Eje Cafetero
title_fullStr	Análisis in silico de genes vinculados a la biosíntesis de ácido indolacético (AIA) asociados a aislamientos bacterianos de suelos agrícolas del Eje Cafetero
title_full_unstemmed	Análisis in silico de genes vinculados a la biosíntesis de ácido indolacético (AIA) asociados a aislamientos bacterianos de suelos agrícolas del Eje Cafetero
title_sort	Análisis in silico de genes vinculados a la biosíntesis de ácido indolacético (AIA) asociados a aislamientos bacterianos de suelos agrícolas del Eje Cafetero
dc.creator.fl_str_mv	Jaramillo Zárate, María José
dc.contributor.advisor.none.fl_str_mv	Rivera Rodríguez, Silvia
dc.contributor.author.none.fl_str_mv	Jaramillo Zárate, María José
dc.subject.spa.fl_str_mv	Ácidos indolacéticos Genes Enzimas Metabolismo BPCV (bacterias promotoras de crecimiento vegetal)
topic	Ácidos indolacéticos Genes Enzimas Metabolismo BPCV (bacterias promotoras de crecimiento vegetal) Indoleacetic Acids Genes Enzymes Metabolism PGPB (Plant Growth Promoting Bacteria)
dc.subject.subjectenglish.spa.fl_str_mv	Indoleacetic Acids Genes Enzymes Metabolism PGPB (Plant Growth Promoting Bacteria)
description	El ácido indolacético (AIA) es una de las principales fitohormonas responsables de la morfogénesis de las plantas y un metabolito secundario bacteriano de interés en las Bacterias Promotoras de Crecimiento Vegetal (BPCV). En las aplicaciones biotecnológicas que se dan en la agricultura, como la formulación de bioinsumos, se requiere de una caracterización amplia y detallada de las propiedades y mecanismos con los que el microorganismo puede impactar positivamente la planta y/o el entorno de esta. No obstante, la biosíntesis del AIA en BPCV no cuenta con una exploración amplia, y la bioinformática representa una herramienta valiosa para develar información sobre este metabolismo de forma integral. En esta investigación se realizó el análisis bioinformático de los cinco morfotipos con mayor producción de AIA dentro del proyecto “Biotecnología Agrícola para Producción de Hortalizas en Risaralda” (Bueno-López, 2023) a partir de las secuencias ARNr 16S y sus genomas homólogos, para comprobar las bases genéticas y moleculares de este proceso metabólico, en conjunto con propiedades genómicas que justifican su potencialidad para procesos biotecnológicos. Se demuestra que las especies homólogas son pertenecientes de Pseudomonas, Burkholderia y Bacillus, géneros relevantes en las BPCV. Se determinó la existencia de los genes para dos rutas biosintéticas dependientes de TRP y cómo su filiación a un mismo grupo de genes homólogos, clusters y relaciones filogenéticas se constituyen desde las regiones conservadas en los genomas de Pseudomonas y Burkholderia, y del enfoque de investigación del AIA para estos dos géneros. Se encuentra que la producción de AIA in vitro en BPCV suele estar asociada a otras actividades promotoras de crecimiento vegetal que suman a la respuesta de la planta hacia el estrés ambiental. Este estudio termina sumándole valor agregado a los candidatos para la propuesta de formulación de un bioinsumo, y destaca la importancia de la aplicación de herramientas in silico en pro del desarrollo agrícola y de los contextos de investigación desde la academia.
publishDate	2024
dc.date.created.none.fl_str_mv	2024-06-12
dc.date.accessioned.none.fl_str_mv	2025-01-20T15:11:11Z
dc.date.available.none.fl_str_mv	2025-01-20T15:11:11Z
dc.type.coar.fl_str_mv	http://purl.org/coar/resource_type/c_7a1f
dc.type.local.spa.fl_str_mv	Tesis de Pregrado
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/bachelorThesis
dc.identifier.uri.none.fl_str_mv	https://hdl.handle.net/10901/30432
url	https://hdl.handle.net/10901/30432
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spelling	Rivera Rodríguez, SilviaJaramillo Zárate, María JoséPereira2025-01-20T15:11:11Z2025-01-20T15:11:11Z2024-06-12https://hdl.handle.net/10901/30432El ácido indolacético (AIA) es una de las principales fitohormonas responsables de la morfogénesis de las plantas y un metabolito secundario bacteriano de interés en las Bacterias Promotoras de Crecimiento Vegetal (BPCV). En las aplicaciones biotecnológicas que se dan en la agricultura, como la formulación de bioinsumos, se requiere de una caracterización amplia y detallada de las propiedades y mecanismos con los que el microorganismo puede impactar positivamente la planta y/o el entorno de esta. No obstante, la biosíntesis del AIA en BPCV no cuenta con una exploración amplia, y la bioinformática representa una herramienta valiosa para develar información sobre este metabolismo de forma integral. En esta investigación se realizó el análisis bioinformático de los cinco morfotipos con mayor producción de AIA dentro del proyecto “Biotecnología Agrícola para Producción de Hortalizas en Risaralda” (Bueno-López, 2023) a partir de las secuencias ARNr 16S y sus genomas homólogos, para comprobar las bases genéticas y moleculares de este proceso metabólico, en conjunto con propiedades genómicas que justifican su potencialidad para procesos biotecnológicos. Se demuestra que las especies homólogas son pertenecientes de Pseudomonas, Burkholderia y Bacillus, géneros relevantes en las BPCV. Se determinó la existencia de los genes para dos rutas biosintéticas dependientes de TRP y cómo su filiación a un mismo grupo de genes homólogos, clusters y relaciones filogenéticas se constituyen desde las regiones conservadas en los genomas de Pseudomonas y Burkholderia, y del enfoque de investigación del AIA para estos dos géneros. Se encuentra que la producción de AIA in vitro en BPCV suele estar asociada a otras actividades promotoras de crecimiento vegetal que suman a la respuesta de la planta hacia el estrés ambiental. Este estudio termina sumándole valor agregado a los candidatos para la propuesta de formulación de un bioinsumo, y destaca la importancia de la aplicación de herramientas in silico en pro del desarrollo agrícola y de los contextos de investigación desde la academia.Universidad Libre seccional Pereira -- Facultad de Ciencias de la Salud, Exactas y Naturales -- MicrobiologíaIndole-3-acetic acid (IAA) is one of the main phytohormones responsible for plant morphogenesis and a bacterial secondary metabolite of interest in Plant Growth Promoting Bacteria (PGPB). Biotechnological applications in agriculture, such as the formulation of biofertilizers, require extensive and detailed characterization of the properties and mechanisms by which the microorganism can positively impact the plant and/or its environment. However, the biosynthesis of IAA in PGPB is not widely explored, and bioinformatics represents a valuable tool to unveil information about this metabolism in a comprehensive manner. In this research, the bioinformatic analysis of the five morphotypes with the highest IAA production within the project “Biotecnología Agrícola para Producción de Hortalizas en Risaralda” (Bueno-López, 2023) is carried out based on 16S rRNA sequences and their homologous genomes, to verify the genetic and molecular basis of this metabolic process, together with genomic properties that justify its potential for biotechnological processes. It is demonstrated that the homologous species belong to Pseudomonas, Burkholderia and Bacillus, relevant genera in PGPB. We determine the existence of genes for two TRP-dependent biosynthetic pathways and how their affiliation to the same homologous gene group, clusters and phylogenetic relationships are constituted from conserved regions in the genomes of Pseudomonas and Burkholderia, and from the research focus of IAA for these two genera. It is found that in vitro IAA production in PGPB is often associated with other plant growth-promoting activities that add to the plant's response to environmental stress. This study ends up adding value to the candidates for the proposed formulation of a biofertilizer and highlights the importance of the application of in silico tools for agricultural development and research contexts from academiaPDFhttp://creativecommons.org/licenses/by-nc-nd/2.5/co/Atribución-NoComercial-SinDerivadas 2.5 Colombiainfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Ácidos indolacéticosGenesEnzimasMetabolismoBPCV (bacterias promotoras de crecimiento vegetal)Indoleacetic AcidsGenesEnzymesMetabolismPGPB (Plant Growth Promoting Bacteria)Análisis in silico de genes vinculados a la biosíntesis de ácido indolacético (AIA) asociados a aislamientos bacterianos de suelos agrícolas del Eje CafeteroIn silico analysis of genes linked to indoleacetic acid (IAA) biosynthesis associated with bacterial isolates from agricultural soils of the Eje Cafetero.Tesis de Pregradoinfo:eu-repo/semantics/bachelorThesishttp://purl.org/coar/resource_type/c_7a1fBueno-López, L. 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Molecular Microbiology, 68(1), 152–172. https://doi.org/10.1111/j.1365-2958.2008.06144.xTHUMBNAILTesis AIA - Jaramillo-Zárate.pdf.jpgTesis AIA - Jaramillo-Zárate.pdf.jpgIM Thumbnailimage/jpeg12288http://repository.unilibre.edu.co/bitstream/10901/30432/5/Tesis%20AIA%20-%20Jaramillo-Z%c3%a1rate.pdf.jpgf9ea05254461c9d0b5bde44b745d14e9MD55Formato autorización biblioteca.pdf.jpgFormato autorización biblioteca.pdf.jpgIM Thumbnailimage/jpeg28674http://repository.unilibre.edu.co/bitstream/10901/30432/6/Formato%20autorizaci%c3%b3n%20biblioteca.pdf.jpg8d6e879be3d189b33e2a6909a8ed8f0aMD56LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repository.unilibre.edu.co/bitstream/10901/30432/4/license.txt8a4605be74aa9ea9d79846c1fba20a33MD54ORIGINALTesis AIA - Jaramillo-Zárate.pdfTesis AIA - Jaramillo-Zárate.pdfapplication/pdf7156403http://repository.unilibre.edu.co/bitstream/10901/30432/1/Tesis%20AIA%20-%20Jaramillo-Z%c3%a1rate.pdfbb80112cfd0142cc70ff2da273fb093dMD51Formato autorización biblioteca.pdfFormato autorización biblioteca.pdfAUTORIZACIÓN PARA LA PUBLICACIÓN DIGITAL DE OBRAS EN EL REPOSITORIO INSTITUCIONAL DE LA UNIVERSIDAD LIBREapplication/pdf279973http://repository.unilibre.edu.co/bitstream/10901/30432/3/Formato%20autorizaci%c3%b3n%20biblioteca.pdfc6e758860154d734e506afcc5dae8e19MD5310901/30432oai:repository.unilibre.edu.co:10901/304322025-01-21 06:01:18.541Repositorio Institucional Unilibrerepositorio@unilibrebog.edu.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

Análisis in silico de genes vinculados a la biosíntesis de ácido indolacético (AIA) asociados a aislamientos bacterianos de suelos agrícolas del Eje Cafetero

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