Optimization of Phycobiliprotein Solubilization from a Thermotolerant Oscillatoria sp

The present study evaluated the effect of multiple variables (drying time, drying temperature, biomass/solvent ratio, glass beads/biomass ratio, extraction time, and extraction speed) in the solubilization of three different phycobiliproteins (C-PC, APC, and PE) from a thermotolerant Oscillatoria sp. The strain was grown in BG11 media (28 ◦C, light: dark cycle of 12:12 h at 100 µmol·m−2 ·s −1 , 20 days) and the experiments were conducted according to a two-level randomized factorial design with six center points (38 runs). Results show that biomass/solvent ratio, glass beads/biomass ratio, and extraction time, are the most significant variables in the extraction of all three proteins, whereas the glass beads/biomass ratio and extraction time significantly affect their purity. The optimized conditions allow a statistical increase in the concentration of C-PC, APC, and PE extracted from the biomass; however, the purity was lower in comparison with the expected value. The latter occurs due to a larger biomass/solvent ratio and longer extraction times, which enhanced the solubility of other hydrophilic metabolites (proteins and carbohydrates, etc.).

• Autores: Barajas Solano, andres F
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2022
• Institución: Universidad Francisco de Paula Santander
• Repositorio: Repositorio Digital UFPS
• Idioma: eng
• OAI Identifier: oai:repositorio.ufps.edu.co:ufps/6535
• Acceso en línea: https://repositorio.ufps.edu.co/handle/ufps/6535; https://doi.org/10.3390/pr10050836
• Palabra clave: surface response methodology; allophycocyanin; phycocyanin; phycoerythrin; drying; biomass/solvent ratio; cell lysis
• Rights: openAccess
• License: © 2022 by the author