Different effect of nitrogen sources in autotrophic and mixotrophic culture of Scenedesmus spfor biomass and carotenoids production using acidic coal mine drainage effluents

In the present study, the conditions for an autotrophic and mixotrophic culture of Scenedesmus sp were established. Effluents from acidic coal mine drains were used to study biomass and carotenoid production, both under autotrophic and mixotrophic conditions in relation to the effect of different so...

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Autores:
Urbina-Suarez, Nestor Andres
Tipo de recurso:
Article of journal
Fecha de publicación:
2021
Institución:
Universidad Francisco de Paula Santander
Repositorio:
Repositorio Digital UFPS
Idioma:
eng
OAI Identifier:
oai:repositorio.ufps.edu.co:ufps/6978
Acceso en línea:
https://repositorio.ufps.edu.co/handle/ufps/6978
Palabra clave:
carotenoids
coal mine acid (MAD)
mixotrophy
cenedesmus sp
wastewater
Rights
openAccess
License
https://creativecommons.org/licenses/by-nc-sa/4.0/
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repository_id_str
dc.title.eng.fl_str_mv Different effect of nitrogen sources in autotrophic and mixotrophic culture of Scenedesmus spfor biomass and carotenoids production using acidic coal mine drainage effluents
dc.title.spa.fl_str_mv Efecto de diferentes fuentes de nitrógeno en el cultivo autotrófico y mixotrófico de Scenedesmussp para la producción de biomasa y carotenoides utilizando efluentes ácidos de drenaje de minas de carbón
title Different effect of nitrogen sources in autotrophic and mixotrophic culture of Scenedesmus spfor biomass and carotenoids production using acidic coal mine drainage effluents
spellingShingle Different effect of nitrogen sources in autotrophic and mixotrophic culture of Scenedesmus spfor biomass and carotenoids production using acidic coal mine drainage effluents
carotenoids
coal mine acid (MAD)
mixotrophy
cenedesmus sp
wastewater
title_short Different effect of nitrogen sources in autotrophic and mixotrophic culture of Scenedesmus spfor biomass and carotenoids production using acidic coal mine drainage effluents
title_full Different effect of nitrogen sources in autotrophic and mixotrophic culture of Scenedesmus spfor biomass and carotenoids production using acidic coal mine drainage effluents
title_fullStr Different effect of nitrogen sources in autotrophic and mixotrophic culture of Scenedesmus spfor biomass and carotenoids production using acidic coal mine drainage effluents
title_full_unstemmed Different effect of nitrogen sources in autotrophic and mixotrophic culture of Scenedesmus spfor biomass and carotenoids production using acidic coal mine drainage effluents
title_sort Different effect of nitrogen sources in autotrophic and mixotrophic culture of Scenedesmus spfor biomass and carotenoids production using acidic coal mine drainage effluents
dc.creator.fl_str_mv Urbina-Suarez, Nestor Andres
dc.contributor.author.none.fl_str_mv Urbina-Suarez, Nestor Andres
dc.subject.proposal.eng.fl_str_mv carotenoids
coal mine acid (MAD)
mixotrophy
cenedesmus sp
wastewater
topic carotenoids
coal mine acid (MAD)
mixotrophy
cenedesmus sp
wastewater
description In the present study, the conditions for an autotrophic and mixotrophic culture of Scenedesmus sp were established. Effluents from acidic coal mine drains were used to study biomass and carotenoid production, both under autotrophic and mixotrophic conditions in relation to the effect of different sources of nitrogen: sodium nitrate, urea and ammonium phosphate. Pollutant removal was determined, such as COD, TOC, heavy metals, ions and total suspended solids. Batch cultures lasted nine days and biomass production, carotenoids and pollutant removal were compared in each condition. The highest biomass concentration reached was 1.2 g/L in the mixotrophic culture with urea, followed by the culture with sodium nitrate of 0.9 g/L in a C/N ratio of 6:1. The highest carotenoid specific concentration was reached in the mixotrophic culture with sodium nitrate, 19.43 mg carotenoids /g biomass. The culture with ammonium phosphate was found to be inhibitory for the growth of the microalgae with the lowest results of all the parameters except for the autotrophic culture where the concentration of carotenoids was very similar to those reached with sodium nitrate and urea. Finally, the mixotrophic culture with urea presented the highest removal percentages, being 86.97% for total iron, 58.29% for chlorides and 92.5% for COD.
publishDate 2021
dc.date.issued.none.fl_str_mv 2021-10-30
dc.date.accessioned.none.fl_str_mv 2024-04-19T15:28:16Z
dc.date.available.none.fl_str_mv 2024-04-19T15:28:16Z
dc.type.spa.fl_str_mv Artículo de revista
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dc.identifier.uri.none.fl_str_mv https://repositorio.ufps.edu.co/handle/ufps/6978
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dc.relation.ispartof.none.fl_str_mv Ingeniería Y Competitividad ISSN: 2027-8284, 2021 vol:24 fasc: 1 págs: 1 - 14, DOI:10.25100/iyc.24i1.11284
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dc.relation.cites.none.fl_str_mv N. A. Urbina-Suárez, «Efecto de diferentes fuentes de nitrógeno en el cultivo autotrófico y mixotrófico de Scenedesmus sp para la producción de biomasa y carotenoides utilizando efluentes ácidos de drenaje de minas de carbón», inycomp, vol. 24, n.º 1, oct. 2021.
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spelling Urbina-Suarez, Nestor Andres3e8ce1897f5d9382bb88755e9ec9cb966002024-04-19T15:28:16Z2024-04-19T15:28:16Z2021-10-30https://repositorio.ufps.edu.co/handle/ufps/697810.25100/iyc.v24i1.11284In the present study, the conditions for an autotrophic and mixotrophic culture of Scenedesmus sp were established. Effluents from acidic coal mine drains were used to study biomass and carotenoid production, both under autotrophic and mixotrophic conditions in relation to the effect of different sources of nitrogen: sodium nitrate, urea and ammonium phosphate. Pollutant removal was determined, such as COD, TOC, heavy metals, ions and total suspended solids. Batch cultures lasted nine days and biomass production, carotenoids and pollutant removal were compared in each condition. The highest biomass concentration reached was 1.2 g/L in the mixotrophic culture with urea, followed by the culture with sodium nitrate of 0.9 g/L in a C/N ratio of 6:1. The highest carotenoid specific concentration was reached in the mixotrophic culture with sodium nitrate, 19.43 mg carotenoids /g biomass. The culture with ammonium phosphate was found to be inhibitory for the growth of the microalgae with the lowest results of all the parameters except for the autotrophic culture where the concentration of carotenoids was very similar to those reached with sodium nitrate and urea. Finally, the mixotrophic culture with urea presented the highest removal percentages, being 86.97% for total iron, 58.29% for chlorides and 92.5% for COD.13 Páginasapplication/pdfengIngeniería Y CompetitividadCali, ColombiaIngeniería Y Competitividad ISSN: 2027-8284, 2021 vol:24 fasc: 1 págs: 1 - 14, DOI:10.25100/iyc.24i1.11284Vol.24 No.1 (2022)131 (2022)124N. A. Urbina-Suárez, «Efecto de diferentes fuentes de nitrógeno en el cultivo autotrófico y mixotrófico de Scenedesmus sp para la producción de biomasa y carotenoides utilizando efluentes ácidos de drenaje de minas de carbón», inycomp, vol. 24, n.º 1, oct. 2021.Esta obra está bajo una licencia internacional Creative Commons Atribución-NoComercial-CompartirIgual 4.0.https://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccessAtribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)http://purl.org/coar/access_right/c_abf2https://revistaingenieria.univalle.edu.co/index.php/ingenieria_y_competitividad/article/view/11284Different effect of nitrogen sources in autotrophic and mixotrophic culture of Scenedesmus spfor biomass and carotenoids production using acidic coal mine drainage effluentsEfecto de diferentes fuentes de nitrógeno en el cultivo autotrófico y mixotrófico de Scenedesmussp para la producción de biomasa y carotenoides utilizando efluentes ácidos de drenaje de minas de carbónArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85carotenoidscoal mine acid (MAD)mixotrophycenedesmus spwastewaterJaimes-Duarte D-L, Soler-Mendoza W, Velasco-Mendoza J, Muñoz-Peñaloza Y, Urbina-Suárez N-A. Characterization chlorophytas microalgae with potential in the production of lipids for biofuels. CTyF - Ciencia, Tecnología y Futuro. 2012;5(1):93-102. https://doi.org/10.29047/01225383.210Hussain F, Shah SZ, Ahmad H, Abubshait SA, Abubshait HA, Laref A, et al. Microalgae an ecofriendly and sustainable wastewater treatment option: Biomass application in biofuel and bio-fertilizer production A review. Renew Sustain Energy Rev. 2021;137:110603. https://doi.org/10.1016/j.rser.2020.110603Solovchenko A, Verschoor AM, Jablonowski ND, Nedbal L. Phosphorus from wastewater to crops: An alternative path involving microalgae. Biotechnol Adv. 2016;34(5):550–64. http://dx.doi.org/10.1016/j.biotechadv.2016.01.002Manhaeghe D, Blomme T, Van Hulle SWH, Rousseau DPL. Experimental assessment and mathematical modelling of the growth of Chlorella vulgaris under photoautotrophic, heterotrophic and mixotrophic conditions. Water Res. 2020;184:116152. https://doi.org/10.1016/j.watres.2020.116152Vidotti ADS, Riaño-Pachón DM, Mattiello L, Giraldi LA, Winck F V., Franco TT. Analysis of autotrophic, mixotrophic and heterotrophic phenotypes in the microalgae Chlorella vulgaris using time-resolved proteomics and transcriptomics approaches. Algal Res. 2020;51:102060. https://doi.org/10.1016/j.algal.2020.102060Poddar N, Sen R, Martin GJO. Glycerol and nitrate utilisation by marine microalgae Nannochloropsis salina and Chlorella sp. and associated bacteria during mixotrophic and heterotrophic growth. Algal Res. 2018;33:298–309. https://doi.org/10.1016/j.algal.2018.06.002Kwon G, Le LT, Jeon J, Noh J, Jang Y, Kang D, et al. Effects of light and mass ratio of microalgae and nitrifiers on the rates of ammonia oxidation and nitrate production. Biochem Eng J. 2020;161:107656. https://doi.org/10.1016/j.bej.2020.107656Scarponi P, Volpi Ghirardini AM, Bravi M, Cavinato C. Evaluation of Chlorella vulgaris and Scenedesmus obliquus growth on pretreated organic solid waste digestate. Waste Manag. 2021;119:235–41. https://doi.org/10.1016/j.wasman.2020.09.047Song M, Pei H. The growth and lipid accumulation of Scenedesmus quadricauda during batch mixotrophic/heterotrophic cultivation using xylose as a carbon source. Bioresour Technol. 2018;263:525–31. https://doi.org/10.1016/j.biortech.2018.05.020Wang X, Zhang MM, Sun Z, Liu SF, Qin ZH, Mou JH, et al. Sustainable lipid and lutein production from Chlorella mixotrophic fermentation by food waste hydrolysate. J Hazard Mater. 2020;400(June):123258. https://doi.org/10.1016/j.jhazmat.2020.123258Ayre JM, Moheimani NR, Borowitzka MA. Growth of microalgae on undiluted anaerobic digestate of piggery effluent with high ammonium concentrations. Algal Res. 2017;24(Part A):218–26. https://doi.org/10.1016/j.algal.2017.03.023Moheimani NR, Webb JP, Borowitzka MA. Bioremediation and other potential applications of coccolithophorid algae: A review. Algal Res. 2012;1(2):120–33. https://doi.org/10.1016/j.algal.2012.06.002Cai T, Park SY, Li Y. Nutrient recovery from wastewater streams by microalgae: Status and prospects. Renew Sustain Energy Rev. 2013;19:360–9. https://doi.org/10.1016/j.rser.2012.11.030Goswami RK, Mehariya S, Verma P, Lavecchia R, Zuorro A. Microalgae-based biorefineries for sustainable resource recovery from wastewater. J Water Process Eng. 2021;40:101747. https://doi.org/10.1016/j.jwpe.2020.101747Feng X, Chen Y, Lv J, Han S, Tu R, Zhou X, et al. Enhanced lipid production by Chlorella pyrenoidosa through magnetic field pretreatment of wastewater and treatment of microalgae-wastewater culture solution: Magnetic field treatment modes and conditions. Bioresour Technol. 2020;306(March):123102. https://doi.org/10.1016/j.biortech.2020.123102Sánchez S, Martínez ME, Espejo MT, Pacheco R, Espinola F, Hodaifa G. Mixotrophic culture of Chlorella pyrenoidosa with olive-mill wastewater as the nutrient medium. J Appl Phycol. 2001;13(5):443–9. https://doi.org/10.1023/A:1011929723586Fu W, Wichuk K, Brynjólfsson S. Developing diatoms for value-added products: Challenges and opportunities. N Biotechnol. 2015;32(6):547–51. https://doi.org/10.1016/j.nbt.2015.03.016Kalra R, Gaur S, Goel M. Microalgae bioremediation: A perspective towards wastewater treatment along with industrial carotenoids production. J Water Process Eng. 2021;40:101794. https://doi.org/10.1016/j.jwpe.2020.101794Dickinson KE, Whitney CG, McGinn PJ. Nutrient remediation rates in municipal wastewater and their effect on biochemical composition of the microalga Scenedesmus sp. AMDD. Algal Res. 2013;2(2):127–34. http://dx.doi.org/10.1016/j.algal.2013.01.009Santana H, Cereijo CR, Teles VC, Nascimento RC, Fernandes MS, Brunale P, et al. Microalgae cultivation in sugarcane vinasse: Selection, growth and biochemical characterization. Bioresour Technol. 2017;228:133–40. http://dx.doi.org/10.1016/j.biortech.2016.12.075Caprio F Di, Altimari P, Iaquaniello G, Toro L, Pagnanelli F. T.obliquus mixotrophic cultivation in treated and untreated olive mill wastewater. Chem Eng Trans. 2018;64(May):625–30. https://doi.org/10.3303/CET1864105Torres DD, Cáceres Sepúlveda S, Roa AL, Suárez Gelvez JH, Urbina Suárez NA. Utilización de microalgas de la división Chlorophyta en el tratamiento biológico de drenajes ácidos de minas de carbón. Rev Colomb Biotecnol. 2017;19(2):95–104. https://doi.org/10.15446/rev.colomb.biote.v19n2.70429Cuéllar-García DJ, Rangel-Basto YA, Urbina-Suarez NA, Barajas-Solano AF, Muñoz-Peñaloza YA. Lipids production from Scenedesmus obliquus through carbon/nitrogen ratio optimization. Journal of Physics: Conference Series. 2019;1388:012043. https://doi.org/10.1088/1742-6596/1388/1/012043Wellburn AR. The Spectral Determination of Chlorophylls a and b, as well as Total Carotenoids, Using Various Solvents with Spectrophotometers of Different Resolution. J Plant Physiol. 1994;144(3):307–13. http://dx.doi.org/10.1016/S0176-1617(11)81192-2Wang J, Zhang S, He C, She Z, Pan X, Li Y, et al. Source identification and component characterization of dissolved organic matter in an acid mine drainage reservoir. Sci Total Environ. 2020; 739:139732. https://doi.org/10.1016/j.scitotenv.2020.139732Song C, Han X, Qiu Y, Liu Z, Li S, Kitamura Y. Microalgae carbon fixation integrated with organic matters recycling from soybean wastewater: Effect of pH on the performance of hybrid system. Chemosphere. 2020;248:126094. https://doi.org/10.1016/j.chemosphere.2020.126094Gallardo Martínez D, Bruguera Amarán N, Díaz Duque JA, Cabrera Díaz I. Drenaje ácido de minas y su influencia en ecosistemas asociados al yacimiento Santa Lucía, Cuba. Rev Iberoam Ambient Sustentabilidad. 2020;3(2):67–81. https://doi.org/10.46380/rias.v3i2.79Arumugam M, Agarwal A, Arya MC, Ahmed Z. Influence of nitrogen sources on biomass productivity of microalgae Scenedesmus bijugatus. Bioresour Technol. 2013;131:246–9. http://dx.doi.org/10.1016/j.biortech.2012.12.159Su Y. Revisiting carbon, nitrogen, and phosphorus metabolisms in microalgae for wastewater treatment. Sci Total Environ. 2021; 762:144590. https://doi.org/10.1016/j.scitotenv.2020.144590Qin L, Liu L, Wang Z, Chen W, Wei D. Efficient resource recycling from liquid digestate by microalgae-yeast mixed culture and the assessment of key gene transcription related to nitrogen assimilation in microalgae. Bioresour Technol. 2018;264(March):90–7. https://doi.org/10.1016/j.biortech.2018.05.061Lafarga T, Clemente I, Garcia-Vaquero M. Carotenoids from microalgae. In: Galanakis CM, editor. Carotenoids: Properties, Processing and Applications. Elsevier Inc.; 2020:149–187. Available from: https://doi.org/10.1016/B978-0-12-817067-0.00005-1Gong M, Bassi A. Carotenoids from microalgae: A review of recent developments. Biotechnol Adv. 2016;34(8):1396–412. http://dx.doi.org/10.1016/j.biotechadv.2016.10.005Park YT, Lee H, Yun HS, Song KG, Yeom SH, Choi J. Removal of metal from acid mine drainage using a hybrid system including a pipes inserted microalgae reactor. Bioresour Technol. 2013;150:242–8. http://dx.doi.org/10.1016/j.biortech.2013.09.136Krishna Samal DP, Sukla LB, Pattanaik A, Pradhan D. Role of microalgae in treatment of acid mine drainage and recovery of valuable metals. Mater Today Proc. 2020;30(Part 2):346–50. https://doi.org/10.1016/j.matpr.2020.02.165ORIGINALEfecto de diferentes fuentes de nitrógeno en el cultivo autotrófico y mixotrófico de Scenedesmussp para la producción de biomasa y carotenoides utilizando efluentes ácidos de drenaje de minas de carbón.pdfEfecto de diferentes fuentes de nitrógeno en el cultivo autotrófico y mixotrófico de Scenedesmussp para la producción de biomasa y carotenoides utilizando efluentes ácidos de drenaje de minas de carbón.pdfapplication/pdf774827https://repositorio.ufps.edu.co/bitstream/ufps/6978/1/Efecto%20de%20diferentes%20fuentes%20de%20nitr%c3%b3geno%20en%20el%20cultivo%20autotr%c3%b3fico%20y%20mixotr%c3%b3fico%20de%20Scenedesmussp%20para%20la%20producci%c3%b3n%20de%20biomasa%20y%20carotenoides%20utilizando%20efluentes%20%c3%a1cidos%20de%20drenaje%20de%20minas%20de%20carb%c3%b3n.pdf6b9bdc4fbe7f16d045cadeff1b4627b7MD51open accessLICENSElicense.txtlicense.txttext/plain; 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diferentes fuentes de nitrógeno en el cultivo autotrófico y mixotrófico de Scenedesmussp para la producción de biomasa y carotenoides utilizando efluentes ácidos de drenaje de minas de carbón.pdf.jpgEfecto de diferentes fuentes de nitrógeno en el cultivo autotrófico y mixotrófico de Scenedesmussp para la producción de biomasa y carotenoides utilizando efluentes ácidos de drenaje de minas de carbón.pdf.jpgGenerated Thumbnailimage/jpeg15013https://repositorio.ufps.edu.co/bitstream/ufps/6978/4/Efecto%20de%20diferentes%20fuentes%20de%20nitr%c3%b3geno%20en%20el%20cultivo%20autotr%c3%b3fico%20y%20mixotr%c3%b3fico%20de%20Scenedesmussp%20para%20la%20producci%c3%b3n%20de%20biomasa%20y%20carotenoides%20utilizando%20efluentes%20%c3%a1cidos%20de%20drenaje%20de%20minas%20de%20carb%c3%b3n.pdf.jpg9a38bbd0a5c64ec1c2e91aa4e2aa6b8aMD54open accessufps/6978oai:repositorio.ufps.edu.co:ufps/69782024-04-20 03:00:30.103An error occurred on the license name.|||https://creativecommons.org/licenses/by-nc-sa/4.0/open accessRepositorio Universidad Francisco de Paula 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 incorporada en las Obras Colectivas.

b.	Distribuir copias o fonogramas de las Obras, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública, incluyéndolas como incorporadas en Obras Colectivas, según corresponda.

c.	Distribuir copias de las Obras Derivadas que se generen, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública.
Los derechos mencionados anteriormente pueden ser ejercidos en todos los medios y formatos, actualmente conocidos o que se inventen en el futuro. Los derechos antes mencionados incluyen el derecho a realizar dichas modificaciones en la medida que sean técnicamente necesarias para ejercer los derechos en otro medio o formatos, pero de otra manera usted no está autorizado para realizar obras derivadas. Todos los derechos no otorgados expresamente por el Licenciante quedan por este medio reservados, incluyendo pero sin limitarse a aquellos que se mencionan en las secciones 4(d) y 4(e).

4. Restricciones.
La licencia otorgada en la anterior Sección 3 está expresamente sujeta y limitada por las siguientes restricciones:

a.	Usted puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra sólo bajo las condiciones de esta Licencia, y Usted debe incluir una copia de esta licencia o del Identificador Universal de Recursos de la misma con cada copia de la Obra que distribuya, exhiba públicamente, ejecute públicamente o ponga a disposición pública. No es posible ofrecer o imponer ninguna condición sobre la Obra que altere o limite las condiciones de esta Licencia o el ejercicio de los derechos de los destinatarios otorgados en este documento. No es posible sublicenciar la Obra. Usted debe mantener intactos todos los avisos que hagan referencia a esta Licencia y a la cláusula de limitación de garantías. Usted no puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra con alguna medida tecnológica que controle el acceso o la utilización de ella de una forma que sea inconsistente con las condiciones de esta Licencia. Lo anterior se aplica a la Obra incorporada a una Obra Colectiva, pero esto no exige que la Obra Colectiva aparte de la obra misma quede sujeta a las condiciones de esta Licencia. Si Usted crea una Obra Colectiva, previo aviso de cualquier Licenciante debe, en la medida de lo posible, eliminar de la Obra Colectiva cualquier referencia a dicho Licenciante o al Autor Original, según lo solicitado por el Licenciante y conforme lo exige la cláusula 4(c).

b.	Usted no puede ejercer ninguno de los derechos que le han sido otorgados en la Sección 3 precedente de modo que estén principalmente destinados o directamente dirigidos a conseguir un provecho comercial o una compensación monetaria privada. El intercambio de la Obra por otras obras protegidas por derechos de autor, ya sea a través de un sistema para compartir archivos digitales (digital file-sharing) o de cualquier otra manera no será considerado como estar destinado principalmente o dirigido directamente a conseguir un provecho comercial o una compensación monetaria privada, siempre que no se realice un pago mediante una compensación monetaria en relación con el intercambio de obras protegidas por el derecho de autor.

c.	Si usted distribuye, exhibe públicamente, ejecuta públicamente o ejecuta públicamente en forma digital la Obra o cualquier Obra Derivada u Obra Colectiva, Usted debe mantener intacta toda la información de derecho de autor de la Obra y proporcionar, de forma razonable según el medio o manera que Usted esté utilizando: (i) el nombre del Autor Original si está provisto (o seudónimo, si fuere aplicable), y/o (ii) el nombre de la parte o las partes que el Autor Original y/o el Licenciante hubieren designado para la atribución (v.g., un instituto patrocinador, editorial, publicación) en la información de los derechos de autor del Licenciante, términos de servicios o de otras formas razonables; el título de la Obra si está provisto; en la medida de lo razonablemente factible y, si está provisto, el Identificador Uniforme de Recursos (Uniform Resource Identifier) que el Licenciante especifica para ser asociado con la Obra, salvo que tal URI no se refiera a la nota sobre los derechos de autor o a la información sobre el licenciamiento de la Obra; y en el caso de una Obra Derivada, atribuir el crédito identificando el uso de la Obra en la Obra Derivada (v.g., "Traducción Francesa de la Obra del Autor Original," o "Guión Cinematográfico basado en la Obra original del Autor Original"). Tal crédito puede ser implementado de cualquier forma razonable; en el caso, sin embargo, de Obras Derivadas u Obras Colectivas, tal crédito aparecerá, como mínimo, donde aparece el crédito de cualquier otro autor comparable y de una manera, al menos, tan destacada como el crédito de otro autor comparable.

d.	Para evitar toda confusión, el Licenciante aclara que, cuando la obra es una composición musical:

i.	Regalías por interpretación y ejecución bajo licencias generales. El Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública o la ejecución pública digital de la obra y de recolectar, sea individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, SAYCO), las regalías por la ejecución pública o por la ejecución pública digital de la obra (por ejemplo Webcast) licenciada bajo licencias generales, si la interpretación o ejecución de la obra está primordialmente orientada por o dirigida a la obtención de una ventaja comercial o una compensación monetaria privada.

ii.	Regalías por Fonogramas. El Licenciante se reserva el derecho exclusivo de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, los consagrados por la SAYCO), una agencia de derechos musicales o algún agente designado, las regalías por cualquier fonograma que Usted cree a partir de la obra (“versión cover”) y distribuya, en los términos del régimen de derechos de autor, si la creación o distribución de esa versión cover está primordialmente destinada o dirigida a obtener una ventaja comercial o una compensación monetaria privada.

e.	Gestión de Derechos de Autor sobre Interpretaciones y Ejecuciones Digitales (WebCasting). Para evitar toda confusión, el Licenciante aclara que, cuando la obra sea un fonograma, el Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública digital de la obra (por ejemplo, webcast) y de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, ACINPRO), las regalías por la ejecución pública digital de la obra (por ejemplo, webcast), sujeta a las disposiciones aplicables del régimen de Derecho de Autor, si esta ejecución pública digital está primordialmente dirigida a obtener una ventaja comercial o una compensación monetaria privada.

5. Representaciones, Garantías y Limitaciones de Responsabilidad.
A MENOS QUE LAS PARTES LO ACORDARAN DE OTRA FORMA POR ESCRITO, EL LICENCIANTE OFRECE LA OBRA (EN EL ESTADO EN EL QUE SE ENCUENTRA) “TAL CUAL”, SIN BRINDAR GARANTÍAS DE CLASE ALGUNA RESPECTO DE LA OBRA, YA SEA EXPRESA, IMPLÍCITA, LEGAL O CUALQUIERA OTRA, INCLUYENDO, SIN LIMITARSE A ELLAS, GARANTÍAS DE TITULARIDAD, COMERCIABILIDAD, ADAPTABILIDAD O ADECUACIÓN A PROPÓSITO DETERMINADO, AUSENCIA DE INFRACCIÓN, DE AUSENCIA DE DEFECTOS LATENTES O DE OTRO TIPO, O LA PRESENCIA O AUSENCIA DE ERRORES, SEAN O NO DESCUBRIBLES (PUEDAN O NO SER ESTOS DESCUBIERTOS). ALGUNAS JURISDICCIONES NO PERMITEN LA EXCLUSIÓN DE GARANTÍAS IMPLÍCITAS, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.

6. Limitación de responsabilidad.
A MENOS QUE LO EXIJA EXPRESAMENTE LA LEY APLICABLE, EL LICENCIANTE NO SERÁ RESPONSABLE ANTE USTED POR DAÑO ALGUNO, SEA POR RESPONSABILIDAD EXTRACONTRACTUAL, PRECONTRACTUAL O CONTRACTUAL, OBJETIVA O SUBJETIVA, SE TRATE DE DAÑOS MORALES O PATRIMONIALES, DIRECTOS O INDIRECTOS, PREVISTOS O IMPREVISTOS PRODUCIDOS POR EL USO DE ESTA LICENCIA O DE LA OBRA, AUN CUANDO EL LICENCIANTE HAYA SIDO ADVERTIDO DE LA POSIBILIDAD DE DICHOS DAÑOS. ALGUNAS LEYES NO PERMITEN LA EXCLUSIÓN DE CIERTA RESPONSABILIDAD, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.

7. Término.

a.	Esta Licencia y los derechos otorgados en virtud de ella terminarán automáticamente si Usted infringe alguna condición establecida en ella. Sin embargo, los individuos o entidades que han recibido Obras Derivadas o Colectivas de Usted de conformidad con esta Licencia, no verán terminadas sus licencias, siempre que estos individuos o entidades sigan cumpliendo íntegramente las condiciones de estas licencias. Las Secciones 1, 2, 5, 6, 7, y 8 subsistirán a cualquier terminación de esta Licencia.

b.	Sujeta a las condiciones y términos anteriores, la licencia otorgada aquí es perpetua (durante el período de vigencia de los derechos de autor de la obra). No obstante lo anterior, el Licenciante se reserva el derecho a publicar y/o estrenar la Obra bajo condiciones de licencia diferentes o a dejar de distribuirla en los términos de esta Licencia en cualquier momento; en el entendido, sin embargo, que esa elección no servirá para revocar esta licencia o que deba ser otorgada , bajo los términos de esta licencia), y esta licencia continuará en pleno vigor y efecto a menos que sea terminada como se expresa atrás. La Licencia revocada continuará siendo plenamente vigente y efectiva si no se le da término en las condiciones indicadas anteriormente.

8. Varios.

a.	Cada vez que Usted distribuya o ponga a disposición pública la Obra o una Obra Colectiva, el Licenciante ofrecerá al destinatario una licencia en los mismos términos y condiciones que la licencia otorgada a Usted bajo esta Licencia.

b.	Si alguna disposición de esta Licencia resulta invalidada o no exigible, según la legislación vigente, esto no afectará ni la validez ni la aplicabilidad del resto de condiciones de esta Licencia y, sin acción adicional por parte de los sujetos de este acuerdo, aquélla se entenderá reformada lo mínimo necesario para hacer que dicha disposición sea válida y exigible.

c.	Ningún término o disposición de esta Licencia se estimará renunciada y ninguna violación de ella será consentida a menos que esa renuncia o consentimiento sea otorgado por escrito y firmado por la parte que renuncie o consienta.

d.	Esta Licencia refleja el acuerdo pleno entre las partes respecto a la Obra aquí licenciada. No hay arreglos, acuerdos o declaraciones respecto a la Obra que no estén especificados en este documento. El Licenciante no se verá limitado por ninguna disposición adicional que pueda surgir en alguna comunicación emanada de Usted. Esta Licencia no puede ser modificada sin el consentimiento mutuo por escrito del Licenciante y Usted.
0000-0002-6954-508X3e8ce1897f5d9382bb88755e9ec9cb96600